Random and exhaustive generation of permutations and cycles

In 1986 S. Sattolo introduced a simple algorithm for uniform random generation of cyclic permutations on a fixed number of symbols. This algorithm is very similar to the standard method for generating a random permutation, but is less well known. We consider both methods in a unified way, and discuss their relation with exhaustive generation methods. We analyse several random variables associated with the algorithms and find their grand probability generating functions, which gives easy access to moments and limit laws.Comment: 9 page

Pultrusion Die Assembly

This invention relates generally to pultrusion die assemblies, and more particularly, to a pultrusion die assembly which incorporates a plurality of functions in order to produce a continuous, thin composite fiber reinforced thermoplastic material. The invention is useful for making high performance thermoplastic composite materials in sheets which can be coiled on a spool and stored for further processing

TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus

Funding: This work was supported by the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001220), the UK Medical Research Council (FC001220), and the Wellcome Trust (FC001200). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Acknowledgments We are indebted to The Francis Crick Institute Flow Cytometry facility, and in particular Bhavik Patel, Graham Preece, Wayne Turnbull and Phil Hobson. We would also like to thank The Francis Crick Institute Procedural Service Section for production of GA lines and Biological Services, especially Trisha Norton, Keith Williams and Adebambo Adekoya for animal husbandry and technical support; to Riccardo Guidi for constructive discussions and technical assistance. We would like to thank Gitta Stockinger and AhR Immunity Laboratory for providing technical support and reagents throughout this study. We also thank Richard Rance and the Wellcome Trust Sanger Institute’s 454 pyrosequencing team for generating 16S rRNA gene data.Peer reviewedPublisher PD

Early Interferon-γ Production in Human Lymphocyte Subsets in Response to Nontyphoidal Salmonella Demonstrates Inherent Capacity in Innate Cells

Background Nontyphoidal Salmonellae frequently cause life-threatening bacteremia in sub-Saharan Africa. Young children and HIV-infected adults are particularly susceptible. High case-fatality rates and increasing antibiotic resistance require new approaches to the management of this disease. Impaired cellular immunity caused by defects in the T helper 1 pathway lead to intracellular disease with Salmonella that can be countered by IFNγ administration. This report identifies the lymphocyte subsets that produce IFNγ early in Salmonella infection. Methodology Intracellular cytokine staining was used to identify IFNγ production in blood lymphocyte subsets of ten healthy adults with antibodies to Salmonella (as evidence of immunity to Salmonella), in response to stimulation with live and heat-killed preparations of the D23580 invasive African isolate of Salmonella Typhimurium. The absolute number of IFNγ-producing cells in innate, innate-like and adaptive lymphocyte subpopulations was determined. Principal Findings Early IFNγ production was found in the innate/innate-like lymphocyte subsets: γδ-T cells, NK cells and NK-like T cells. Significantly higher percentages of such cells produced IFNγ compared to adaptive αβ-T cells (Student's t test, P<0.001 and ≤0.02 for each innate subset compared, respectively, with CD4+- and CD8+-T cells). The absolute numbers of IFNγ-producing cells showed similar differences. The proportion of IFNγ-producing γδ-T cells, but not other lymphocytes, was significantly higher when stimulated with live compared with heat-killed bacteria (P<0.0001). Conclusion/Significance Our findings indicate an inherent capacity of innate/innate-like lymphocyte subsets to produce IFNγ early in the response to Salmonella infection. This may serve to control intracellular infection and reduce the threat of extracellular spread of disease with bacteremia which becomes life-threatening in the absence of protective antibody. These innate cells may also help mitigate against the effect on IFNγ production of depletion of Salmonella-specific CD4+-T lymphocytes in HIV infection

DPAC 2013: Dixon Learning Center

The University of New Mexico\u27s Design and Planning Assistance Center (DPAC) was engaged to investigate the site of the Embudo Valley Library and explore alternative designs for its development. In an eight-week planning and design effort the architecture and landscape architecture student teams embraced the challenge and devised five distinct possible visions for the site and its buildings.https://digitalrepository.unm.edu/dpac_projects/1012/thumbnail.jp

Abating low-frequency noise using encapsulated gas bubbles

Air bubbles may be used to reduce radiated underwater noise. Two modalities of sound attenuation by air bubbles were shown to provide a reduction in radiated sound: bubble acoustic resonance damping and acoustic impedance mismatching. The bubbles used for acoustic resonance damping were manifested using gas-filled containers coupled to a support, and the acoustic impedance mismatching bubbles were created using a cloud of freely-rising bubbles, which were both used to surround an underwater sound source.Board of Regents, University of Texas Syste

A Spectroscopic Survey of the Fields of 28 Strong Gravitational Lenses: The Group Catalog

With a large, unique spectroscopic survey in the fields of 28 galaxy-scale strong gravitational lenses, we identify groups of galaxies in the 26 adequately-sampled fields. Using a group finding algorithm, we find 210 groups with at least five member galaxies; the median number of members is eight. Our sample spans redshifts of 0.04 $\le z_{grp} \le$ 0.76 with a median of 0.31, including 174 groups with $0.1 < z_{grp} < 0.6$. Groups have radial velocity dispersions of 60 $\le \sigma_{grp} \le$ 1200 km s$^{-1}$ with a median of 350 km s$^{-1}$. We also discover a supergroup in field B0712+472 at $z =$ 0.29 consisting of three main groups. We recover groups similar to $\sim$ 85% of those previously reported in these fields within our redshift range of sensitivity and find 187 new groups with at least five members. The properties of our group catalog, specifically 1) the distribution of $\sigma_{grp}$, 2) the fraction of all sample galaxies that are group members, and 3) the fraction of groups with significant substructure, are consistent with those for other catalogs. The distribution of group virial masses agrees well with theoretical expectations. Of the lens galaxies, 12 of 26 (46%) (B1422+231, B1600+434, B2114+022, FBQS J0951+2635, HE0435-1223, HST J14113+5211, MG0751+2716, MGJ1654+1346, PG 1115+080, Q ER 0047-2808, RXJ1131-1231, and WFI J2033-4723) are members of groups with at least five galaxies, and one more (B0712+472) belongs to an additional, visually identified group candidate. There are groups not associated with the lens that still are likely to affect the lens model; in six of 25 (24%) fields (excluding the supergroup), there is at least one massive ($\sigma_{grp} \ge$ 500 km s$^{-1}$) group or group candidate projected within 2$^{\prime}$ of the lens.Comment: 87 pages, 8 figures, a version of this was published in Ap

The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 angstrom resolution

Maturation of tRNA precursors into functional tRNA molecules requires trimming of the primary transcript at both the 5' and 3' ends. Cleavage of nucleotides from the 3' stem of tRNA precursors, releasing nucleotide diphosphates, is accomplished in Bacillus by a phosphate-dependent exoribonuclease, Rph. The crystal structure of this enzyme from B. anthracis has been solved by molecular replacement to a resolution of 1.7 angstrom and refined to an R factor of 19.3%. There is one molecule in the asymmetric unit; the crystal packing reveals the assembly of the protein into a hexamer arranged as a trimer of dimers. The structure shows two sulfate ions bound in the active-site pocket, probably mimicking the phosphate substrate and the phosphate of the 3'-terminal nucleotide of the tRNA precursor. Three other bound sulfate ions point to likely RNA-binding sites