Magnetic Fields in Star-Forming Molecular Clouds I. The First Polarimetry of OMC-3 in Orion A

The first polarimetric images of the OMC-3 region of the Orion A filamentary molecular cloud are presented. Using the JCMT, we have detected polarized thermal emission at 850 microns from dust along a 6' length of the dense filament. The polarization pattern is highly ordered and is aligned with the filament throughout most of the region. The plane-of-sky magnetic field direction is perpendicular to the measured polarization. The mean percentage polarization is 4.2% with a 1 sigma dispersion of 1%. This region is part of the integral-shaped filament, and active star formation is ongoing along its length. The protostellar outflow directions do not appear to be consistently correlated with the direction of the plane-of-sky field or the filament structure itself. Depolarization toward the filament center, previously detected in many other star-forming cores and protostars, is also evident in our data. (abstract abridged)Comment: 9 pages plus 2 figures (1 colour); accepted for publication in the March 10, 2000 issue (vol. 531 #2) of The Astrophysical Journa

Variable responses of human and non-human primate gut microbiomes to a Western diet

BACKGROUND: The human gut microbiota interacts closely with human diet and physiology. To better understand the mechanisms behind this relationship, gut microbiome research relies on complementing human studies with manipulations of animal models, including non-human primates. However, due to unique aspects of human diet and physiology, it is likely that host-gut microbe interactions operate differently in humans and non-human primates. RESULTS: Here, we show that the human microbiome reacts differently to a high-protein, high-fat Western diet than that of a model primate, the African green monkey, or vervet (Chlorocebus aethiops sabaeus). Specifically, humans exhibit increased relative abundance of Firmicutes and reduced relative abundance of Prevotella on a Western diet while vervets show the opposite pattern. Predictive metagenomics demonstrate an increased relative abundance of genes associated with carbohydrate metabolism in the microbiome of only humans consuming a Western diet. CONCLUSIONS: These results suggest that the human gut microbiota has unique properties that are a result of changes in human diet and physiology across evolution or that may have contributed to the evolution of human physiology. Therefore, the role of animal models for understanding the relationship between the human gut microbiota and host metabolism must be re-focused.P40 OD010965 - NIH HHS; P40 RR019963 - NCRR NIH HHS; P51 OD011132 - NIH HHS; R01 RR016300 - NCRR NIH HHS; 5R01RR016300 - NCRR NIH HH

Wild state secrets: ultra-sensitive measurement of micro-movement can reveal internal processes in animals

Assessment of animal internal "state" - which includes hormonal, disease, nutritional, and emotional states - is normally considered the province of laboratory work, since its determination in animals in the wild is considered more difficult. However, we show that accelerometers attached externally to animals as diverse as elephants, cockroaches, and humans display consistent signal differences in micro-movement that are indicative of internal state. Originally used to elucidate the behavior of wild animals, accelerometers also have great potential for highlighting animal actions, which are considered as responses stemming from the interplay between internal state and external environment. Advances in accelerometry may help wildlife managers understand how internal state is linked to behavior and movement, and thus clarify issues ranging from how animals cope with the presence of newly constructed roads to how diseased animals might change movement patterns and therefore modulate disease spread

Cytochrome P4501A biomarker indication of the timeline of chronic exposure of Barrow’s goldeneyes to residual Exxon Valdez oil

Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Marine Pollution Bulletin 62 (2011): 609-614, doi:10.1016/j.marpolbul.2010.11.015.We examined hepatic EROD activity, as an indicator of CYP1A induction, in Barrow's goldeneyes captured in areas oiled during the 1989 Exxon Valdez spill and those from nearby unoiled areas. We found that average EROD activity differed between areas during 2005, although the magnitude of the difference was reduced relative to a previous study from 1996/97, and we found that areas did not differ by 2009. Similarly, we found that the proportion of individuals captured from oiled areas with elevated EROD activity ( 2 times unoiled average) declined from 41% in winter 1996/97 to 10% in 2005 and 15% in 2009. This work adds to a body of literature describing the timelines over which vertebrates were exposed to residual Exxon Valdez oil and indicates that, for Barrow's goldeneyes in Prince William Sound, exposure persisted for many years with evidence of substantially reduced exposure by 2 decades after the spill.This research was supported primarily by the Exxon Valdez Oil Spill Trustee Council

Reflections on the Cost of Low-Cost Whole Genome Sequencing: Framing the Health Policy Debate

The cost of whole genome sequencing is dropping rapidly. There has been a great deal of enthusiasm about the potential for this technological advance to transform clinical care. Given the interest and significant investment in genomics, this seems an ideal time to consider what the evidence tells us about potential benefits and harms, particularly in the context of health care policy. The scale and pace of adoption of this powerful new technology should be driven by clinical need, clinical evidence, and a commitment to put patients at the centre of health care policy

Identification and Characterization of Trimethylamine-N-oxide Uptake and Efflux Transporters

Trimethylamine-N-oxide (TMAO) is a recently identified predictor of cardiovascular and chronic kidney disease. TMAO is primarily generated through gut-microbiome mediated conversion of dietary choline and carnitine to TMA, which is converted to TMAO by hepatic flavin monooxygenase 3 (FMO3) and subsequently undergoes renal elimination. We investigated the role of uptake and efflux drug transporters in TMAO disposition in vitro and in vivo. After screening a large array of uptake transporters, we show organic cation transporter 2 (OCT2) is the key transporter for TMAO cellular uptake. In Oct1/2 knockout mice, we observed increased plasma TMAO levels with reduced renal retention, suggesting the importance of Oct2 in facilitating the uptake of TMAO into renal tubular cells in vivo. Multiple transporters of the ATP-binding cassette (ABC) family, including ABCG2 (BCRP) and ABCB1 (MDR1), were capable of TMAO efflux. In human subjects, clinical, dietary, and pharmacogenetic covariates were evaluated for contribution to TMAO levels in a cohort of dyslipidemic patients (n = 405). Interestingly, genetic variation in ABCG2, but not other transporters, appeared to play a role in modulating TMAO exposure

Blast from the past : a study of decades-old fungal cultures resolves a long-standing tree disease mystery

DATA AVAILABILITY : The sequences that were generated for this study have been deposited at the NCBI. The accession numbers will be made available upon publication.A root disease in plantations of Pinus radiata and Pinus pinaster, where trees died in distinct patches, was present in the Western Cape province of South Africa during the 1970s and 1980s. Phytophthora cinnamomi was initially believed to be the cause, but the disease was later ascribed to the insect-associated fungus Leptographium serpens, a fungal species residing in the Ophiostomatales. Doubt regarding the cause of the disease was raised in a later study due to the fact that most Leptographium spp., particularly those that colonise ray parenchyma tissues, which is the case for L. serpens, are not typically primary disease agents. In this study, cultures of an unidentified sterile fungus collected from the dying trees were revived and identified using DNA sequencing methods, which were not available when the disease was first studied. These cultures were identified as the pyrophillic pathogen Rhizina undulata, well-known to cause patch death of conifers in South Africa and elsewhere in the world. While the patches of dying trees no longer exist and the disease cannot be newly studied, it is most likely that the tree death originally thought to be caused by L. serpens was due primarily to R. undulata. The study provides a vivid example of the value of preserving cultures of fungi for later study and the power of modern techniques to identify fungal pathogens.The members of the Tree Protection Co-operative Programme (TPCP) and the National Research Foundation (NRF) in South Africa. Open access funding provided by University of Pretoria.https://link.springer.com/journal/42161hj2023BiochemistryForestry and Agricultural Biotechnology Institute (FABI)GeneticsMicrobiology and Plant PathologyPlant Production and Soil Scienc