Disclosure of Children's Positive Serostatus to Family and Nonfamily Members: Informal Caregivers in Togo, West Africa

This study examined the structural constraints to disclosure of children's positive serostatus among informal caregivers to family and nonfamily members in Togo. It drew on two data sources, one qualitative and the other quantitative. Qualitative data showed that caregivers cautiously disclosed child's positive serostatus for fear of being stigmatized and discriminated against as well as to protect the children from being stigmatized. Binary regression analyses revealed that different factors influenced reasons for disclosure of a child's serostatus. For instance, while caregivers' serostatus and number of children significantly influenced disclosure for financial support, disclosure of a child's serostatus for spiritual support was strongly affected by education and religion. These results shed light on factors and reasons for disclosure among caregivers. This knowledge is important because different types of programs and advice should be given to caregivers with specific reason(s) for disclosure instead of creating a “one-size-fits all” program for all caregivers

Sialic Acid Mutarotation Is Catalyzed by the Escherichia coli β-Propeller Protein YjhT

The acquisition of host-derived sialic acid is an important virulence factor for some bacterial pathogens, but in vivo this sugar acid is sequestered in sialoconjugates as the {alpha}-anomer. In solution, however, sialic acid is present mainly as the β-anomer, formed by a slow spontaneous mutarotation. We studied the Escherichia coli protein YjhT as a member of a family of uncharacterized proteins present in many sialic acid-utilizing pathogens. This protein is able to accelerate the equilibration of the {alpha}- and β-anomers of the sialic acid N-acetylneuraminic acid, thus describing a novel sialic acid mutarotase activity. The structure of this periplasmic protein, solved to 1.5Å resolution, reveals a dimeric 6-bladed unclosed β-propeller, the first of a bacterial Kelch domain protein. Mutagenesis of conserved residues in YjhT demonstrated an important role for Glu-209 and Arg-215 in mutarotase activity. We also present data suggesting that the ability to utilize {alpha}-N-acetylneuraminic acid released from complex sialoconjugates in vivo provides a physiological advantage to bacteria containing YjhT

Cardiovascular disease biomarkers are associated with declining renal function in type 2 diabetes

Aims/hypothesis: We investigated whether biochemical cardiovascular risk factors and/or markers of subclinical cardiovascular disease were associated with the development of reduced renal function in people with type 2 diabetes. Methods: A cohort of 1066 Scottish men and women aged 60–74 years with type 2 diabetes from the Edinburgh Type 2 Diabetes Study were followed up for a median of 6.7 years. New-onset reduced renal function was defined as two eGFRs <60 ml−1 min−1 (1.73 m)−2 at least 3 months apart with a > 25% decline from baseline eGFR. Ankle brachial pressure index (ABI), N-terminal pro-B-type natriuretic peptide (NT-proBNP) and high-sensitivity troponin T (hsTnT) were measured at baseline. Pulse wave velocity (PWV) and carotid intima media thickness were measured 1 year into follow-up. Data were analysed using Cox proportional hazards models. Results: A total of 119 participants developed reduced renal function during follow-up. ABI, PWV, NT-proBNP and hsTnT were all associated with onset of decline in renal function following adjustment for age and sex. These associations were attenuated after adjustment for additional diabetes renal disease risk factors (systolic BP, baseline eGFR, albumin:creatinine ratio and smoking pack-years), with the exception of hsTnT which remained independently associated (HR 1.51 [95% CI 1.22, 1.87]). Inclusion of hsTnT in a predictive model improved the continuous net reclassification index by 0.165 (0.008, 0.286). Conclusions/interpretation: Our findings demonstrate an association between hsTnT, a marker of subclinical cardiac ischaemia, and subsequent renal function decline. Further research is required to establish the predictive value of hsTnT and response to intervention

Report on Interstate 476 (The Blue Route)

For fifty years Pennsylvanians have been discussing the idea of constructing a new north-south highway in the central part of Delaware County, the suburban county immediately west of the City of Philadelphia. Such a highway originally was conceived as a parkway. The notion never got very far until the mid-1950\u27s. When the federal Interstate Highway System was conceived, a facility in central Delaware County was proposed to connect the Pennsylvania Turnpike (I-276) in Plymouth Meeting, Montgomery County, with the Delaware Expressway (I-95) in Ridley Township, Delaware County. This facility was designated I-476. As conceived, it would constitute the western part of a planned circumferential freeway network around Philadelphia. I-476 is commonly known as the Blue Route and also as the Mid County Expressway. As an interstate highway project, 90% of the cost of I-476 would be covered by federal interstate highway funds allocated to the Pennsylvania Department of Transportation (PennDOT) by the Federal Highway Administration (FHWA). The remaining 10% of the project\u27s cost would be paid for by PennDOT (either construction bonds or revenues from the State Motor Fund). Construction of the Blue Route commenced in 1967 but all con­struction on the main stretch of the road--the 16.9 mile section be­ tween I-95 and the Schuylkill Expressway (I-76)--was halted in 1973. Construction of this portion cannot resume until the project undergoes an environmental impact assessment under the provisions of federal law. The project also must undergo a so-called 4(f) assessment analyzing the facility\u27s impact on parkland and historical resources. The requirements of these federal statutes are summarized in an appen­dix to this report. Because of the uncertainty surrounding completion of the Blue Route, and because the project has aroused considerable controversy in Delaware County, U.S. Representative Robert W. Edgar in March 1977 organized a Transportation Advisory Committee to un­dertake a comprehensive analysis of the project. [...] This report is submitted to the Congressman as the Committee\u27s recommendation

Optimization of inhomogeneous electron correlation factors in periodic solids

A method is presented for the optimization of one-body and inhomogeneous two-body terms in correlated electronic wave functions of Jastrow-Slater type. The most general form of inhomogeneous correlation term which is compatible with crystal symmetry is used and the energy is minimized with respect to all parameters using a rapidly convergent iterative approach, based on Monte Carlo sampling of the energy and fitting energy fluctuations. The energy minimization is performed exactly within statistical sampling error for the energy derivatives and the resulting one- and two-body terms of the wave function are found to be well-determined. The largest calculations performed require the optimization of over 3000 parameters. The inhomogeneous two-electron correlation terms are calculated for diamond and rhombohedral graphite. The optimal terms in diamond are found to be approximately homogeneous and isotropic over all ranges of electron separation, but exhibit some inhomogeneity at short- and intermediate-range, whereas those in graphite are found to be homogeneous at short-range, but inhomogeneous and anisotropic at intermediate- and long-range electron separation.Comment: 23 pages, 15 figures, 1 table, REVTeX4, submitted to PR

Teaching and Assessing Engineering Design Thinking with Virtual Internships and Epistemic Network Analysis

An engineering workforce of sufficient size and quality is essential for addressing significant global challenges such as climate change, world hunger, and energy demand. Future generations of engineers will need to identify challenging issues and design innovative solutions. To prepare young people to solve big and increasingly global problems, researchers and educators need to understand how we can best educate young people to use engineering design thinking. In this paper, we explore virtual internships, online simulations of 21st-century engineering design practice, as one method for teaching engineering design thinking. To assess the engineering design thinking, we use epistemic network analysis (ENA), a tool for measuring complex thinking as it develops over time based on discourse analysis. The combination of virtual internships and ENA provides opportunities for students to engage in authentic engineering design, potentially receive concurrent feedback on their engineering design thinking, and develop the identity, values, and ways of thinking of professional engineers

Multifrequency Observations of Radio Pulse Broadening and Constraints on Interstellar Electron Density Microstructure

We have made observations of 98 low-Galactic-latitude pulsars to measure pulse broadening caused by multipath propagation through the interstellar medium. Data were collected with the 305-m Arecibo telescope at four radio frequencies between 430 and 2380 MHz. We used a CLEAN-based algorithm to deconvolve interstellar pulse broadening from the measured pulse shapes. We employed two distinct pulse broadening functions (PBFs): PBF$_1$ is appropriate for a thin screen of scattering material between the Earth and a pulsar, while PBF$_2$ is appropriate for scattering material uniformly distributed along the line of sight from the Earth to a pulsar. We found that some observations were better fit by PBF$_1$ and some by PBF$_2$. Pulse broadening times ($\tau_d$) are derived from fits of PBFs to the data, and are compared with the predictions of a smoothed model of the Galactic electron distribution. Several lines of sight show excess broadening, which we model as clumps of high density scattering material. A global analysis of all available data finds that the pulse broadening scales with frequency, $\nu$, as \taud \propto\nu^{-\alpha} where $\alpha\sim 3.9\pm 0.2$. This is somewhat shallower than the value $\alpha=4.4$ expected from a Kolmogorov medium, but could arise if the spectrum of turbulence has an inner cutoff at $\sim$300--800 km. A few objects follow particularly shallow scaling laws (the mean scaling index \meanalpha \sim 3.1 \pm 0.1 and $\sim 3.8 \pm 0.2$ respectively for the case of PBF$_1$ and PBF$_2$), which may arise from large scale refraction or from the truncation of scattering screens transverse to the Earth--pulsar line of sight.Comment: Accepted for publication in the Astrophysical Journal; 32 pages, 11 figure

Common Host Responses in Murine Aerosol Models of Infection Caused by Highly Virulent Gram-Negative Bacteria from the Genera Burkholderia, Francisella and Yersinia

This is the final version. Available on open access from MDPI via the DOI in this recordContent includes material subject to © Crown copyright (2019), Dstl. This material is licensed under the terms of the Open Government Licence except where otherwise stated. To view this licence, visit: http://www.nationalarchives.gov.uk/doc/open-government-licence/version/3 or write to the Information Policy Team, The National Archives, Kew, London TW9 4DU, or email: [email protected] virulent bacterial pathogens cause acute infections which are exceptionally difficult to treat with conventional antibiotic therapies alone. Understanding the chain of events that are triggered during an infection of a host has the potential to lead to new therapeutic strategies. For the first time, the transcriptomic responses within the lungs of Balb/C mice have been compared during an acute infection with the intracellular pathogens Burkholderia pseudomallei, Francisella tularensis and Yersinia pestis. Temporal changes were determined using RNAseq and a bioinformatics pipeline; expression of protein was also studied from the same sample. Collectively it was found that early transcriptomic responses within the infected host were associated with the (a) slowing down of critical cellular functions, (b) production of circulatory system components, (c) lung tissue integrity, and (d) intracellular regulatory processes. One common molecule was identified, Errfi1 (ErbB receptor feedback inhibitor 1); upregulated in response to all three pathogens and a potential novel marker of acute infection. Based upon the pro-inflammatory responses observed, we sought to synchronise each infection and report that 24 h p.i. of B. pseudomallei infection closely aligned with 48 h p.i. of infection with F. tularensis and Y. pestis. Post-transcriptional modulation of RANTES expression occurred across all pathogens, suggesting that these infections directly or indirectly modulate cell trafficking through chemokine expression/detection. Collectively, this unbiased NGS approach has provided an in-depth characterisation of the host transcriptome following infection with these highly virulent pathogens ultimately aiding in the development of host-directed therapies as adjuncts or alternatives to antibiotic treatment

A Precise Water Abundance Measurement for the Hot Jupiter WASP-43b

The water abundance in a planetary atmosphere provides a key constraint on the planet's primordial origins because water ice is expected to play an important role in the core accretion model of planet formation. However, the water content of the Solar System giant planets is not well known because water is sequestered in clouds deep in their atmospheres. By contrast, short-period exoplanets have such high temperatures that their atmospheres have water in the gas phase, making it possible to measure the water abundance for these objects. We present a precise determination of the water abundance in the atmosphere of the 2 $M_\mathrm{Jup}$ short-period exoplanet WASP-43b based on thermal emission and transmission spectroscopy measurements obtained with the Hubble Space Telescope. We find the water content is consistent with the value expected in a solar composition gas at planetary temperatures (0.4-3.5x solar at 1 $\sigma$ confidence). The metallicity of WASP-43b's atmosphere suggested by this result extends the trend observed in the Solar System of lower metal enrichment for higher planet masses.Comment: Accepted to ApJL; this version contains three supplemental figures that are not included in the published paper. See also our companion paper "Thermal structure of an exoplanet atmosphere from phase-resolved emission spectroscopy" by Stevenson et a