White Screen/White Noise: Racism on the Internet

The Internet is critical for disseminating information, but it does not discriminate against information it carries. Hate speech and racist representations proliferate in social media, online news comment sections and community forums. What can information workers do to counteract those messages? How can critical race consciousness enhance on- and off-line library services? This article attempts to explore how information in the age of rapid technology innovation contributes to structural racism and what librarians and other information professionals can do about it

White Screen/White Noise: Racism and the Internet

The Internet is critical for disseminating information, but it does not discriminate against information it carries. Hate speech and racist representations proliferate in social media, online news comment sections and community forums. What can information workers do to counteract those messages? How can critical race consciousness enhance on- and off-line library services? This article attempts to explore how information in the age of rapid technology innovation contributes to structural racism and what librarians and other information professionals can do about it

Canyon formation constraints on the discharge of catastrophic outburst floods of Earth and Mars

Catastrophic outburst floods carved amphitheater-headed canyons on Earth and Mars, and the steep headwalls of these canyons suggest that some formed by upstream headwall propagation through waterfall erosion processes. Because topography evolves in concert with water flow during canyon erosion, we suggest that bedrock canyon morphology preserves hydraulic information about canyon-forming floods. In particular, we propose that for a canyon to form with a roughly uniform width by upstream headwall retreat, erosion must occur around the canyon head, but not along the sidewalls, such that canyon width is related to flood discharge. We develop a new theory for bedrock canyon formation by megafloods based on flow convergence of large outburst floods toward a horseshoe-shaped waterfall. The model is developed for waterfall erosion by rock toppling, a candidate erosion mechanism in well fractured rock, like columnar basalt. We apply the model to 14 terrestrial (Channeled Scablands, Washington; Snake River Plain, Idaho; and Ásbyrgi canyon, Iceland) and nine Martian (near Ares Vallis and Echus Chasma) bedrock canyons and show that predicted flood discharges are nearly 3 orders of magnitude less than previously estimated, and predicted flood durations are longer than previously estimated, from less than a day to a few months. Results also show a positive correlation between flood discharge per unit width and canyon width, which supports our hypothesis that canyon width is set in part by flood discharge. Despite lower discharges than previously estimated, the flood volumes remain large enough for individual outburst floods to have perturbed the global hydrology of Mars

Mutational Analysis of the Rotavirus NSP4 Enterotoxic Domain that Binds to Caveolin-1

Background: Rotavirus (RV) nonstructural protein 4 (NSP4) is the first described viral enterotoxin, which induces early secretory diarrhea in neonatal rodents. Our previous data show a direct interaction between RV NSP4 and the structural protein of caveolae, caveolin-1 (cav-1), in yeast and mammalian cells. The binding site of cav-1 mapped to the NSP4 amphipathic helix, and led us to examine which helical face was responsible for the interaction. Methods: A panel of NSP4 mutants were prepared and tested for binding to cav-1 by yeast two hybrid and direct binding assays. The charged residues of the NSP4 amphipathic helix were changed to alanine (NSP446-175-ala6); and three residues in the hydrophobic face were altered to charged amino acids (NSP446-175-HydroMut). In total, twelve mutants of NSP4 were generated to define the cav-1 binding site. Synthetic peptides corresponding to the hydrophobic and charged faces of NSP4 were examined for structural changes by circular dichroism (CD) and diarrhea induction by a neonatal mouse study. Results: Mutations of the hydrophilic face (NSP446-175-Ala6) bound cav-1 akin to wild type NSP4. In contrast, disruption of the hydrophobic face (NSP446-175-HydroMut) failed to bind cav-1. These data suggest NSP4 and cav-1 associate via a hydrophobic interaction. Analyses of mutant synthetic peptides in which the hydrophobic residues in the enterotoxic domain of NSP4 were altered suggested a critical hydrophobic residue. Both NSP4HydroMut112-140, that contains three charged amino acids (aa113, 124, 131) changed from the original hydrophobic residues and NSP4AlaAcidic112-140 that contained three alanine residues substituted for negatively charged (aa114, 125, 132) amino acids failed to induce diarrhea. Whereas peptides NSP4wild type 112 −140 and NSP4AlaBasic112-140 that contained three alanine substituted for positively charged (aa115, 119, 133) amino acids, induced diarrhea. Conclusions: These data show that the cav-1 binding domain is within the hydrophobic face of the NSP4 amphipathic helix. The integrity of the helical structure is important for both cav-1 binding and diarrhea induction implying a connection between NSP4 functional and binding activities

Model for the formation of single-thread rivers in barren landscapes and implications for pre-Silurian and martian fluvial deposits

Flume experiments and field observations show that bank vegetation promotes the formation of narrow and deep single‐thread channels by strengthening riverbanks. Consistent with this idea, the pre‐Silurian fluvial record generally consists of wide monotonous sand bodies often interpreted as deposits of shallow braided rivers, whereas single‐thread rivers with muddy floodplains become more recognizable in Silurian and younger rocks. This shift in the architecture of fluvial deposits has been interpreted as reflecting the rise of single‐thread rivers enabled by plant life. The deposits of some single‐thread rivers, however, have been recognized in pre‐Silurian rocks, and recent field studies have identified meandering rivers in modern unvegetated environments. Furthermore, single‐thread‐river deposits have been identified on Mars, where macroscopic plants most likely never evolved. Here, we seek to understand the formation of those rarely recognized and poorly characterized single‐thread rivers in unvegetated landscapes. Specifically, we quantitatively explore the hypothesis that cohesive muddy banks alone may enable the formation of single‐thread rivers in the absence of plants. We combine open‐channel hydraulics and a physics‐based erosion model applicable to a variety of bank sediments to predict the formation of unvegetated single‐thread rivers. Consistent with recent flume experiments and field observations, results indicate that single‐thread rivers may form readily within muddy banks. Our model has direct implications for the quantification of riverbank strengthening by vegetation, understanding the hydraulic geometry of modern and ancient unvegetated rivers, interpreting pre‐Silurian fluvial deposits, and unraveling the hydrologic and climate history of Mars

Postnatal mental health and mothers' processing of infant emotion: an eye-tracking study

BACKGROUND: Postnatal mental illnesses are associated with less maternal sensitivity. Differences in how mothers with and without mental illness process infant emotions could explain this. People with mental illness in non-perinatal populations show certain processing patterns when viewing emotional faces, but it is not clear whether these patterns are present in mothers. OBJECTIVE: Compared to mothers without affective symptoms (anxiety, depression and post-traumatic stress). Task 1 aimed to identify whether mothers with affective symptoms show an attentional bias towards negative infant faces; and Task 2 aimed to identify whether mothers with affective symptoms look less at infants' eyes compared to eyes and mouth. DESIGN: An experimental design was used in two tasks to answer the research objective. METHODS: Mothers with affective symptoms (n = 23) and without affective symptoms (n = 47) had their eye movements tracked whilst: Task 1, viewing pairs of infant faces; and Task 2, viewing morphed infant faces. RESULTS: In Task 1 mothers with affective symptoms were more likely to fixate first on neutral faces when the choice was between negative and neutral. In Task 2, no differences were found between groups. CONCLUSIONS: The findings from Task 1 are unexpected given previous research. More research is needed to identify potential explanations for this

Formation of sinuous ridges by inversion of river-channel belts in Utah, USA, with implications for Mars

Sinuous ridges are important landforms on the surface of Mars that show promise for quantifying ancient martian surface hydrology. Morphological similarity of these ridges to river channels in planform led to a hypothesis that ridges are topographically inverted river channels, or “inverted channels”, formed due to an erosion-resistant channel-filling material that preserved a snapshot of the channel geometry in inverted relief due to differential erosion. An alternative deposit-inversion hypothesis proposes that ridges represent exhumed river-channel belts, with geometries that reflect the lateral migration and vertical aggradation of rivers over significant geologic time, rather than the original channel geometry. To investigate these hypotheses we studied sinuous ridges within the Cretaceous Cedar Mountain Formation near Green River, Utah, USA. Ridges in Utah extend for hundreds of meters, are up to 120 m wide, and stand up to 39 m above the surrounding plain. Ridges are capped by sandstone bodies 3–10 m thick that contain dune- and bar-scale inclined stratification, which we interpret as eroded remnants of channel belts that record the migration and aggradation of single-thread, sand-bedded rivers, rather than channel fills that can preserve the original channel geometry. Caprocks overlie mudstones and thinner sandstone beds that are interpreted as floodplain deposits, and in cases additional channel-belt sandstones are present lower in the ridge stratigraphy. Apparent networks from branching ridges typically represent discrete sandstone bodies that cross at different stratigraphic levels rather than a coeval river network. Ridge-forming sandstone bodies also have been narrowed during exhumation by cliff retreat and bisected by fluvial erosion. Using a large compilation of channel-belt geometries on Earth and our measurements of ridges in Utah, we propose that caprock thickness is the most reliable indicator of paleo-channel geometry, and can be used to reconstruct river depth and discharge. In contrast, channel lateral migration and caprock erosion during exhumation make ridge breadth an uncertain proxy for channel width. An example in Aeolis Dorsa, Mars, illustrates that river discharge estimates based solely on caprock width may differ significantly from estimates based on caprock thickness. Overall, our study suggests that sinuous ridges are not inverted channel fills, but rather reflect exhumation of a thick stratigraphic package of stacked channel belts and overbank deposits formed from depositional rivers over significant geologic time

Rotavirus NSP4: Cell Type-dependent Transport Kinetics to the Exofacial Plasma Membrane and Release from Intact Infected Cells

Background Rotavirus NSP4 localizes to multiple intracellular sites and is multifunctional, contributing to RV morphogenesis, replication and pathogenesis. One function of NSP4 is the induction of early secretory diarrhea by binding surface receptors to initiate signaling events. The aims of this study were to determine the transport kinetics of NSP4 to the exofacial plasma membrane (PM), the subsequent release from intact infected cells, and rebinding to naïve and/or neighboring cells in two cell types. Methods Transport kinetics was evaluated using surface-specific biotinylation/streptavidin pull-downs and exofacial exposure of NSP4 was confirmed by antibody binding to intact cells, and fluorescent resonant energy transfer. Transfected cells similarly were monitored to discern NSP4 movement in the absence of infection or other viral proteins. Endoglycosidase H digestions, preparation of CY3- or CY5- labeled F(ab)2 fragments, confocal imaging, and determination of preferential polarized transport employed standard laboratory techniques. Mock-infected, mock-biotinylated and non-specific antibodies served as controls. Results Only full-length (FL), endoglycosidase-sensitive NSP4 was detected on the exofacial surface of two cell types, whereas the corresponding cell lysates showed multiple glycosylated forms. The C-terminus of FL NSP4 was detected on exofacial-membrane surfaces at different times in different cell types prior to its release into culture media. Transport to the PM was rapid and distinct yet FL NSP4 was secreted from both cell types at a time similar to the release of virus. NSP4-containing, clarified media from both cells bound surface molecules of naïve cells, and imaging showed secreted NSP4 from one or more infected cells bound neighboring cell membranes in culture. Preferential sorting to apical or basolateral membranes also was distinct in different polarized cells. Conclusions The intracellular transport of NSP4 to the PM, translocation across the PM, exposure of the C-terminus on the cell surface and subsequent secretion occurs via an unusual, complex and likely cell-dependent process. The exofacial exposure of the C-terminus poses several questions and suggests an atypical mechanism by which NSP4 traverses the PM and interacts with membrane lipids. Mechanistic details of the unconventional trafficking of NSP4, interactions with host-cell specific molecules and subsequent release require additional study

Canyon formation constraints on the discharge of catastrophic outburst floods of Earth and Mars

Catastrophic outburst floods carved amphitheater-headed canyons on Earth and Mars, and the steep headwalls of these canyons suggest that some formed by upstream headwall propagation through waterfall erosion processes. Because topography evolves in concert with water flow during canyon erosion, we suggest that bedrock canyon morphology preserves hydraulic information about canyon-forming floods. In particular, we propose that for a canyon to form with a roughly uniform width by upstream headwall retreat, erosion must occur around the canyon head, but not along the sidewalls, such that canyon width is related to flood discharge. We develop a new theory for bedrock canyon formation by megafloods based on flow convergence of large outburst floods toward a horseshoe-shaped waterfall. The model is developed for waterfall erosion by rock toppling, a candidate erosion mechanism in well fractured rock, like columnar basalt. We apply the model to 14 terrestrial (Channeled Scablands, Washington; Snake River Plain, Idaho; and Ásbyrgi canyon, Iceland) and nine Martian (near Ares Vallis and Echus Chasma) bedrock canyons and show that predicted flood discharges are nearly 3 orders of magnitude less than previously estimated, and predicted flood durations are longer than previously estimated, from less than a day to a few months. Results also show a positive correlation between flood discharge per unit width and canyon width, which supports our hypothesis that canyon width is set in part by flood discharge. Despite lower discharges than previously estimated, the flood volumes remain large enough for individual outburst floods to have perturbed the global hydrology of Mars