Rock against Reagan : The punk movement, cultural hegemony, and Reaganism in the eighties

Despite scholars’ growing interest in the cultural movement known as punk, there has been a lack of focus on the movement’s relationship to its historical context. Punk meant rebellion, and this research looks at how the rebellion of the American punk movement during the eighties [1978 to 1992], was aimed at the president Ronald Reagan. Their dissent, however, was not only directed towards Reagan, but the culture that he encompassed. Under this influence, American culture gave way to Reaganism: a culture of individualism, greed, power, and symbols. Adding to the work by historian Dewar MacLeod and others who have focused on punk rock in major urban settings like New York and Los Angeles, this project will look beyond those centers. By using fanzines (underground magazine publications), songs, oral histories, interviews, video recordings, and popular news media sources, this research explores: the influence of the Atlantic littoral in the development and politics of punk; a regional focus on the Midwest and how the youth of the region created a punk community in an unlikely location; the “Rock Against Reagan” tour and political activism within punk that challenged Reagan’s bid for a second presidential term; censorship and the charges of “distributing harmful matter to a minor” against the Dead Kennedys’ 1985 Frankenchrist album; and how the punk movement adapted to a new decade, and what can be seen as a new century in the 1990s. Filling a missing gap for research, this project helps to better understand the punk movement, Reagan, and the culture of the eighties

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

\u3ci\u3eDrosophila\u3c/i\u3e Muller F Elements Maintain a Distinct Set of Genomic Properties Over 40 Million Years of Evolution

The Muller F element (4.2 Mb, ~80 protein-coding genes) is an unusual autosome of Drosophila melanogaster; it is mostly heterochromatic with a low recombination rate. To investigate how these properties impact the evolution of repeats and genes, we manually improved the sequence and annotated the genes on the D. erecta, D. mojavensis, and D. grimshawi F elements and euchromatic domains from the Muller D element. We find that F elements have greater transposon density (25–50%) than euchromatic reference regions (3–11%). Among the F elements, D. grimshawi has the lowest transposon density (particularly DINE-1: 2% vs. 11–27%). F element genes have larger coding spans, more coding exons, larger introns, and lower codon bias. Comparison of the Effective Number of Codons with the Codon Adaptation Index shows that, in contrast to the other species, codon bias in D. grimshawi F element genes can be attributed primarily to selection instead of mutational biases, suggesting that density and types of transposons affect the degree of local heterochromatin formation. F element genes have lower estimated DNA melting temperatures than D element genes, potentially facilitating transcription through heterochromatin. Most F element genes (~90%) have remained on that element, but the F element has smaller syntenic blocks than genome averages (3.4–3.6 vs. 8.4–8.8 genes per block), indicating greater rates of inversion despite lower rates of recombination. Overall, the F element has maintained characteristics that are distinct from other autosomes in the Drosophila lineage, illuminating the constraints imposed by a heterochromatic milieu