The prototype colliding-wind pinwheel WR 104

Results from the most extensive study of the time-evolving dust structure around the prototype "Pinwheel" nebula WR 104 are presented. Encompassing 11 epochs in three near-infrared filter bandpasses, a homogeneous imaging data set spanning more than 6 years (or 10 orbits) is presented. Data were obtained from the highly successful Keck Aperture Masking Experiment, which can recover high fidelity images at extremely high angular resolutions, revealing the geometry of the plume with unprecedented precision. Inferred properties for the (unresolved) underlying binary and wind system are orbital period 241.5 +/- 0.5 days and angular outflow velocity of 0.28 +/- 0.02 mas/day. An optically thin cavity of angular size 13.3 +/- 1.4 mas was found to lie between the central binary and the onset of the spiral dust plume. Rotational motion of the wind system induced by the binary orbit is found to have important ramifications: entanglement of the winds results in strong shock activity far downstream from the nose of the bowshock. The far greater fraction of the winds participating in the collision may play a key role in gas compression and the nucleation of dust at large radii from the central binary and shock stagnation point. Investigation of the effects of radiative braking pointed towards significant modifications of the simple hydrostatic colliding wind geometry, extending the relevance of this phenomena to wider binary systems than previously considered. Limits placed on the maximum allowed orbital eccentricity of e < 0.06 argue strongly for a prehistory of tidal circularization in this system. Finally we discuss the implications of Earth's polar (i < 16 deg) vantage point onto a system likely to host supernova explosions at future epochs.Comment: 35 pages, 8 figures, Accepted for publication in Astrophysical Journa

Sexual dysfunction among married couples living in Kumasi metropolis, Ghana

<p>Abstract</p> <p>Background</p> <p>Sexuality and its manifestation constitute some of the most complex of human behaviour and its disorders are encountered in community. Sexual dysfunction is more prevalent in women than in men. While studies examining sexual dysfunction among males and females in Ghana exist, there are no studies relating sexual problems in males and females as dyadic units. This study therefore investigated the prevalence and type of sexual disorders among married couples.</p> <p>Method</p> <p>The study participants consisted of married couples between the ages of 19 and 66 living in the province of Kumasi, Ghana. Socio-demographic information and Golombok-Rust Inventory of Sexual Satisfaction (GRISS) questionnaires were administered to 200 couples who consented to take part in the study. All 28 questions of the GRISS are answered on a five-point (Likert type) scale from "always", through "usually', "sometimes", and "hardly ever", to "never". Responses are summed up to give a total raw score ranging from 28-140. The total score and subscale scores are transformed using a standard nine point scale, with high scores indicating greater problems. Scores of five or more are considered to indicate SD. The study was conducted between July and September 2010.</p> <p>Results</p> <p>Out of a total of 200 married couples, 179 completed their questionnaires resulting in a response rate of 89.5%. The mean age of the participating couples as well as the mean duration of marriage was 34.8 ± 8.6 years and 7.8 ± 7.6 years respectively. The husbands (37.1 ± 8.6) were significantly older (p < 0.0001) than their corresponding wives (32.5 ± 7.9). After adjusting for age, 13-18 years of marriage life poses about 10 times significant risk of developing SD compared to 1-6 years of married life among the wives (OR: 10.8; CI: 1.1 - 49.1; p = 0.04). The total scores (6.0) as well as the percentage above the cut-off (59.2) obtained by the husbands compared to the total score (6.2) and the percentage above cut-off (61.5) obtained by the wives, indicates the likely presence of sexual dysfunction. The prevalence of impotence and premature ejaculation were 60.9% and 65.4% respectively from this study and the prevalence of vaginismus and anorgasmia were 69.3% and 74.9% respectively. The highest prevalence of SD subscales among the men was dissatisfaction with sexual act followed by infrequency, whereas the highest among the women was infrequency followed by anorgasmia. Dissatisfaction with sexual intercourse among men correlated positively with anorgasmia and wife's non-sensuality and infrequency of sex.</p> <p>Conclusion</p> <p>The prevalence of sexual dysfunction in married couples is comparable to prevalence rates in the general male and female population and is further worsened by duration of marriage. This could impact significantly on a couple's self-esteem and overall quality of life.</p

Binding of Hyaluronan to the Native Lymphatic Vessel Endothelial Receptor LYVE-1 Is Critically Dependent on Receptor Clustering and Hyaluronan Organization

The lymphatic endothelial receptor LYVE-1 has been implicated in both uptake of hyaluronan (HA) from tissue matrix and in facilitating transit of leukocytes and tumor cells through lymphatic vessels based largely on in vitro studies with recombinant receptor in transfected fibroblasts. Curiously, however, LYVE-1 in lymphatic endothelium displays little if any binding to HA in vitro, and this has led to the conclusion that the native receptor is functionally silenced, a feature that is difficult to reconcile with its proposed in vivo functions. Nonetheless, as we reported recently, LYVE-1 can function as a receptor for HA-encapsulated Group A streptococci and mediate lymphatic dissemination in mice. Here we resolve these paradoxical findings and show that the capacity of LYVE-1 to bind HA is strictly dependent on avidity, demanding appropriate receptor self-association and/or HA multimerization. In particular, we demonstrate the prerequisite of a critical LYVE-1 threshold density and show that HA binding may be elicited in lymphatic endothelium by surface clustering with divalent LYVE-1 mAbs. In addition, we show that cross-linking of biotinylated HA in streptavidin multimers or supramolecular complexes with the inflammation-induced protein TSG-6 enables binding even in the absence of LYVE-1 cross-linking. Finally, we show that endogenous HA on the surface of macrophages can engage LYVE-1, facilitating their adhesion and transit across lymphatic endothelium. These results reveal LYVE-1 as a low affinity receptor tuned to discriminate between different HA configurations through avidity and establish a new mechanistic basis for the functions ascribed to LYVE-1 in matrix HA binding and leukocyte trafficking in vivo

LYVE-1 and hyaluronan - a molecular velcro

The lymphatic system comprises a network of vessels whose primary functions are the maintenance of extracellular fluid balance and the transport of antigen-presenting cells from the periphery to the lymph nodes, thus facilitating immunological surveillance of the tissues and activation of adaptive immunity. In malignant disease, the lymphatics are both a route for dissemination and a reservoir for metastatic cancers such as cutaneous melanoma and breast carcinoma, where lymph node involvement is an early indicator of prognosis. Yet, despite such obvious importance in disease, the fundamental biology of the lymphatics is poorly understood and critical mechanisms such as those underlying trafficking of dendritic and tumour cells have been largely overlooked. The focus of this thesis is lymphatic vessel endothelial hyaluronan receptor LYVE-1, and the regulation of its binding to the extracellular matrix glycosaminoglycan, hyaluronan (HA). Found selectively on the surface of lymphatic vessels, but sharing many of the features of the leukocyte homing receptor CD44, LYVE-1 appears a likely candidate for regulation of lymphatic trafficking during the stage at which cells migrate into the vessel. However, the precise function of LYVE-1 in recent years has remained enigmatic, not least because the native receptor is subject to post-translational modification with sialic acid, with the effect that HA binding is inhibited in lymphatic endothelium. The results of this thesis demonstrate that sialylation of LYVE-1 is not a short term regulatory modification, but rather a longer term mechanism that imposes a requirement for higher order receptor complex formation or HA crosslinking to achieve stable binding. This implies that native LYVE-1 is an active HA binding protein even when sialylated, with implications for our understanding of the role played by the receptor in HA uptake and metabolism, and transmigration of lymphatic endothelium by migratory leukocytes. Like CD44, HA binding to LYVE-1 is dependent on multivalent interactions between receptor and ligand, and may be enhanced by processes that increase the avidity of the interaction. Here for the first time it is shown that the native LYVE-1 molecule on lymphatic endothelium may be activated to bind HA following clustering of the receptor, or presentation of HA in a cross-linked form, such as that resulting from incubation with the HA binding protein TSG-6.</p

Beagle Gulf benthic survey : characterisation of soft substrates

"This project was carried out with the support of Environment Australia, under the Ocean Rescue 2000 program (project G011/93)" -- title page.Made available via the Publications (Legal Deposit) Act 2004 (NT