On the design and simulation of an airlift loop bioreactor with microbubble generation by fluidic oscillation

Microbubble generation by a novel fluidic oscillator driven approach is analyzed, with a view to identifying the key design elements and their differences from standard approaches to airlift loop bioreactor design. The microbubble generation mechanism has been shown to achieve high mass transfer rates by the decrease of the bubble diameter, by hydrodynamic stabilization that avoids coalescence increasing the bubble diameter, and by longer residence times offsetting slower convection. The fluidic oscillator approach also decreases the friction losses in pipe networks and in nozzles/diffusers due to boundary layer disruption, so there is actually an energetic consumption savings in using this approach over steady flow. These dual advantages make the microbubble generation approach a promising component of a novel airlift loop bioreactor whose design is presented here. The equipment, control system for flow and temperature, and the optimization of the nozzle bank for the gas distribution system are presented. (C) 2009 The Institution of Chemical Engineers. Published by Elsevier B.V All rights reserved

Aerator design for microbubble generation

Fine bubbles are a key component in improving the performance of gas-liquid reactors, particularly in situations where reactions are mass transfer limited. Many aerator types exist for different reactor applications; however conventional aerators are mostly suited to coarse bubble generation. A new aerator suitable for microbubble generation by fluidic oscillation has been designed and tested with the view of getting a uniform bubble distribution across the aerator. Microbubbles generated from various membrane pore sizes and oscillation frequencies were characterized for this aerator to determine the optimum operating parameters. It was evident that the introduction of a flow distributor plate to the plenum chamber improved gas distribution from the inlet to the porous membrane leading to uniform bubble generation across the entire aerator The resultant average bubble size from this new design under oscillatory flow was found to be approximately 2-3 times the membrane pore size. This outcome has a great potential to promote the efficiency of multiphase reactors where mass transfer plays a key role

Microflotation performance for algal separation

The performance of microflotation, dispersed air flotation with microbubble clouds with bubble size about 50 µm, for algae separation using fluidic oscillation for microbubble generation is investigated. This fluidic oscillator converts continuous air supply into oscillatory flow with a regular frequency to generate bubbles of the scale of the exit pore. Bubble characterization results showed that average bubble size generated under oscillatory air flow state was 86 µm, approximately twice the size of the diffuser pore size of 38 µm. In contrast, continuous air flow at the same rate through the same diffusers yielded an average bubble size of 1,059 µm, 28 times larger than the pore size. Following microbubble generation, the separation of algal cells under fluidic oscillator generated microbubbles was investigated by varying metallic coagulant types, concentration and pH. Best performances were recorded at the highest coagulant dose (150 mg/L) applied under acidic conditions (pH 5). Amongst the three metallic coagulants studied, ferric chloride yielded the overall best result of 99.2% under the optimum conditions followed closely by ferric sulfate (98.1%) and aluminum sulfate with 95.2%. This compares well with conventional dissolved air flotation (DAF) benchmarks, but has a highly turbulent flow, whereas microflotation is laminar with several orders of magnitude lower energy density. Biotechnol. Bioeng. 2012; 109:1663–1673. © 2012 Wiley Periodicals, Inc

Evaporation dynamics of microbubbles

Until recently, generating clouds of microbubbles was a relatively expensive proposition, with the smallest bubbles requiring high energy density from either the saturation–nucleation mechanism or Venturi effect. Due to the expense of processing with microbubbles, exploration of the acceleration effects of microbubbles for physico-chemical processes are largely unstudied, particularly those that are combined effects. In this paper, the trade-off between heat transfer and evaporation on the microbubble interface are explored, largely by computational modelling but supported by some experimental evidence. The hypothesis is that both processes are inherently transient, but that during short residence times, vaporization is favoured, while at longer residence times, sensible heat transfer dominates and results in re-condensation of the initially vaporized liquid. The computational model address how thin a layer thickness will result in the maximum absolute vaporization, after which sensible heat transfer condenses the vapour as the bubble cools. This maximum vaporization layer thickness is estimated to be a few hundred microns, on the order of a few microbubble diameters at most. If the maximum vaporization estimate and the contact time necessary to achieve it are accurately estimated, these are engineering design features needed to design a vaporizing system to achieve maximum removal of vapour with minimum heat transfer. The modelling work presented here should be considered in light of the humidification experiments also conducted which showed the exit air at 100% saturation, but increasing gas temperature with decreasing layer height, and decreasing water temperature with decreasing layer height, all of which are consistent with the predictions of the computational model

Experimental investigation of helicity in turbulent swirling jet using dual-plane dye laser PIV technique

This paper reports a new method of generating two light sheets using a dye laser system and the use of this dual-plane dye laser system to analyse average helicity and energy dissipation in a turbulent swirling flow. The dual-plane PIV system that was used in this study consisted of three cameras and a single frequency Nd:YAG laser, which was used to generate two parallel light sheet planes with differing wavelengths(colour). The method of generating two different light sheet wavelengths using a single laser source is an innovative and new technique. Stereoscopic PIV measurements were obtained in one plane with the use of two CCD cameras, and standard PIV measurements were obtained in the other plane with the use of one CCD camera. The light scattered by the particles on two different light sheets were separated using appropriate optical filters. The measurements obtained were used to estimate the components of the velocity gradient tensor. The tensor components were then used to determine the average vorticity components and helicity quantities of the fluid that was investigated. To determine the average turbulent kinetic energy dissipation, the continuity equation was used to infer the out-of-plane gradient of the out-of-plane velocity. From the analysis of the results, it was found that regions with high helicity were correlated with regions of high turbulent kinetic energy dissipation. © 2008 Springer-Verlag

Space Station Freedom automation and robotics: An assessment of the potential for increased productivity

This report presents the results of a study performed in support of the Space Station Freedom Advanced Development Program, under the sponsorship of the Space Station Engineering (Code MT), Office of Space Flight. The study consisted of the collection, compilation, and analysis of lessons learned, crew time requirements, and other factors influencing the application of advanced automation and robotics, with emphasis on potential improvements in productivity. The lessons learned data collected were based primarily on Skylab, Spacelab, and other Space Shuttle experiences, consisting principally of interviews with current and former crew members and other NASA personnel with relevant experience. The objectives of this report are to present a summary of this data and its analysis, and to present conclusions regarding promising areas for the application of advanced automation and robotics technology to the Space Station Freedom and the potential benefits in terms of increased productivity. In this study, primary emphasis was placed on advanced automation technology because of its fairly extensive utilization within private industry including the aerospace sector. In contrast, other than the Remote Manipulator System (RMS), there has been relatively limited experience with advanced robotics technology applicable to the Space Station. This report should be used as a guide and is not intended to be used as a substitute for official Astronaut Office crew positions on specific issues

An Evaluation of The Effectiveness of Adaptive Histogram Equalization for Contrast Enhancement

Adaptive Histogram Equalization (AHE), a method of contrast enhancement which is sensitive to local spatial information in an image, has been proposed as a solution to the problem of the inability of ordinary display devices to depict the full dynamic intensity range in some medical images. This method is automatic, reproducible, and simultaneously displays most of the information contained in the grey-scale contrast of the image. However, it has not been known whether the use of AHE causes the loss of diagnostic information relative to the commonly-used method intensity windowing. In the current work, AHE and intensity windowing are compared using psychophysical observer studies. In studies performed at North Carolina Memorial Hospital, experienced radiologists were shown clinical CT images of the chest. Into some of the images, appropriate artificial lesion were introduced; the physicians were then shown the images processed with both AHE and intensity windowing. They were asked to assess the probability that as given image contained the artificial lesion, and their accurate was measured. The results of these experiments shown that for this particular diagnostic task, there was no significant difference in the ability of the two methods to depict luminance contrast; thus, further evaluation of AHE using controlled clinical trials is indicated

Intensification of yeast production with microbubbles

Yeast requires and consumes a high amount of oxygen rapidly during growth. Maintaining yeast cultures under sufficient aeration, however, is a significant challenge in yeast propagation. Due to their high surface area, microbubbles are more efficient in mass transfer than coarse bubbles. The performance of an airlift loop bioreactor equipped with a fluidic oscillator generated microbubbles in yeast propagation is presented here. The approach is compared with a conventional bubble generation method that produces coarse bubbles. Dosing with microbubbles transferred more oxygen to the cultures, achieving non-zero dissolved O2 levels and consequently, eliminating the starvation state of yeast in contrast to coarse bubble sparging. The average cell growth yield obtained under microbubble sparging reached 0.31 mg/h (±0.02) while 0.22 mg/h (±0.01) was recorded for cells grown with coarse bubbles during the log phase. The percent difference in average growth yield after 6 hours was 18%. Additionally, the use of microbubbles in yeast harvest from growth medium proved effective, yielding >99% cell recovery. The result of this study is crucial for the biofuel industry but also, the food, nutraceutical and pharmaceutical industry for which end product purity is premium

Effects of transport and phase equilibrium on fast, nearly irreversible reactive extraction

Abstract Integration of reaction and separation can be exploited to drive reversible reactions in the direction of the desired product using multiphase flow contacting. In the case of nearly irreversible, fast reactions, however, the dynamics of the product have little influence on the reactor efficiency in say liquid-liquid reactive extraction. A similar intensification in reaction efficiency to reactive separation can be achieved by exploiting phase equilibrium or asymmetry in mass transfer rates of the reactants. Here, a model for two-layer biphasic flow and homogeneous reaction is proposed for co-current reactive extraction, demonstrating that localization and intensification of reaction occurs in the region between the entrance and crossover. Crossover occurs if the reactant in stoichiometric deficit preferentially populates the reacting phase due to sufficient imbalance in either mass transfer coefficients or phase equilibrium. We develop an infinite Peclet number (convection dominates over bulk diffusion) model that indicates that crossover occurs when for fast, irreversible reactions. u 0 and v 0 are initial charges to the tubular reactor, the 's are mass transfer coefficients for each side of the fluid interface and the h's are Henry's Law coefficients for reactants U and V . The interpretation of this formula is that if v 0 > u 0 , then crossover will occur if the overall mass transfer rate of U is faster than the overall mass transfer rate for V . Downstream of the crossover point, the reactant in stoichiometric excess also dominates the reacting phase due to relative exhaustion of the more-mobile component. A finite Peclet number theory for fast, irreversible reaction shows that the above formula is a conservative limit for crossover-if it holds, crossover will occur regardless of the Peclet number. A formula for the larger parametric region for crossover with finite Peclet number is derived. Verification that crossover is achieved is found by finite-element numerical analysis of the full governing equations. Both theory and numerical analysis predict localization and intensification of the reaction due to crossover. Crossover sets the length scale as approximately two and a half crossover lengths for completed reaction for sufficiently high Peclet number with strong kinetic asymmetry. The theory predicts that taking the ratio of inlet concentrations S = u 0 /v 0 to be the critical value at fixed physical parameters for mass transfer and phase equilibrium maximizes localization and reactor efficiency. Similarly, the kinetic asymmetry should be as large as possible to exploit the benefits of crossover.

Design of an airlift loop bioreactor and pilot scales studies with fluidic oscillator induced microbubbles for growth of a microalgae Dunaliella salina

This study was conducted to test the feasibility of growing microalgae on steel plant exhaust gas, generated from the combustion of offgases from steel processing, which has a high CO content. Two field trials of batch algal biomass growth, mediated by microbubble transfer processes in an airlift loop bioreactor showed only steady growth of biomass with 100% survival rate. The gas analysis of CO uptake in the 2200L bioreactor showed a specific uptake rate of 0.1g/L/h, an average 14% of the CO available in the exhaust gas with a 23% composition of CO. This uptake led to a steady production of chlorophyll and total lipid constituency in the bioreactor, and an accelerating exponential growth rate of biomass, with a top doubling time of 1.8days. The gas analysis also showed anti-correlation of CO uptake and O production, which along with the apparent stripping of the O to the equilibrium level by the microbubbles, strongly suggests that the bioreactor is not mass transfer limited, nor O inhibited. Removing O inhibition results in high growth rates and high density of biomass. © 2011 Elsevier Ltd