Exact norm-conserving stochastic time-dependent Hartree-Fock

We derive an exact single-body decomposition of the time-dependent Schroedinger equation for N pairwise-interacting fermions. Each fermion obeys a stochastic time-dependent norm-preserving wave equation. As a first test of the method we calculate the low energy spectrum of Helium. An extension of the method to bosons is outlined.Comment: 21 pages, 3 figures, LaTeX fil

Patient perspectives and experiences of remote consultations in people receiving kidney care: A scoping review

Background: The COVID-19 pandemic resulted in a rapid and sometimes chaotic change in how clinical care was delivered for people living with kidney disease, with increased reliance on digital technologies and the introduction of remote services. Objective: To conduct a scoping review of studies about peoples’ experiences and perspectives in receipt of remote consultations for kidney care. Methods: Using Arksey and O’Malley’s (2005) framework, three databases (CINAHL, MEDLINE, and PsycINFO) were searched simultaneously on EBSCO The search included studies published in English from August 2010 to August 2021. Results: Eight cross-sectional studies met the scoping review criteria (two quantitative, two mixed-method, and four qualitative). Four themes were identified: overall satisfaction with remote services, benefits to patients (convenience, involvement in care, and patient safety), barriers to remote consultations (technical difficulties, digital literacy, loss of interpersonal communication, existing patient-practitioner relationship, and access to technology), and patients’ concerns (need for physical examination, privacy, and confidentiality). Conclusion: Remote consultations confer multiple advantages to patients; therefore, remote consultations should be offered as an option to patients living with kidney disease beyond the COVID-19 pandemic. However, there are several barriers to remote consultation that need to be addressed and understood before implementing remote care long-term. Future research should examine the impact of remote consultations on people living with kidney disease from under-served groups to identify barriers and ensure their suitability and accessibility to the wider population for a more patient-centred approach to kidney care

Studies on the synthesis of ribonucleotide homopolymers

Previous work has shown that cytoplasmic fractions of animal cells are capable of the incorporation of ribonucleoside triphosphates into RNA. The present investigation was carried out to elucidate the mechanism and properties of such enzymes in rat liver cells. Female rats were anaesthetised, the abdomens opened, the livers perfused, and then removed. Enzyme fractions were prepared by differential centrifugation of liver homogenates. The incorporation of radioactive labelled ribonucleoside triphosphates into polyribonucleotides catalysed by such fractions was measured. The microsomal fraction was most active in incorporating (a-32P) UTP into polyribonucleotides. Such activity required a pH of 7.5-8.0 and the addition of Mg++ rather then Mn++ ions. The uptake of (a--32P) UTP was promoted presence of ATP, GTP and CTP, and an ATP generating system. While the addition of RNA stimulated the reaction and ribonuclease completely abolished the reaction, the addition of DNA, deoxy- ribonuclease or actinomycin had no effect on the uptake of (a-32P) UTP. It was concluded that incorporation of ribonucleoside triphosphates was by an RNA-, rather than a DNA- dependent reaction. Alkaline hydrolysis of the reaction products after (a-32P) UTP incorporation, end measurement of the radio-activity in the 2'(3') monophosphates so obtained, indicated that the main product was sequences of uridylic acid. It was not possible, however, to exclude hetero-polymer formation. All four ribonucleoside triphosphates were incorporated into acid insoluble products. In no case was maximum incorporation obtained in the presence of all four ribonucleoside triphosphates, reducing the probability of heteropolymer formation. The microsomes were fractionated into rough surfaced vesicles, smooth surfaced vesicles and free ribosomes. No activity was recovered from the smooth surfaced vesicle fraction. Activity in the rough surfaced vesicle fraction, appeared to be due to contamination by a supernatant fraction. An enzyme fraction with very high specific activity was recovered with the free ribosomes. Washing the ribosomes in dense sucrose solutions and isolation of the free ribosome fraction from media containing high concentrations of EDTA had no effect on the activity. It could be demonstrated unequivocally that the incorporation of UTP represented addition of seven to eight residues of uridylic acid to the 2'(3') ends of pre-existing RNA chains. ATP end CTP, but not GTP, were also incorporated into polyribonucleotides. Following the uptake of 3H-UTP, the nucleic acids were extracted from reaction mixtures, Using a combination of fractionation by sucrose gradient centrifugation and on columns of G-100 Sephadex, it was possible to exclude ribosomal RNA, 5S-RNA and transfer RNA as endogenous primers. This left the interesting possibility that messenger RNA acted as acceptors for the homopolymer sequences. The biological significance and the relationship of the free ribosomal enzyme(s) to other known enzymes of polynucleotide metabolism are discussed in some detail. The uptake of ribonucleoside triphosphates by a supernatant fraction was investigated. The uptake of UTP required. the presence of Mg++ ions and en added RNA primer. The incorporation of UTP could adequately be described as terminal addition of one to two ribonucleotides to the 2'(3') ends of pre-existing RNA primers. The enzyme (s) appeared to require free 2' (3') hydroxyl groups on the primer molecules. The significance of this activity is discussed

Suppression of decoherence via strong intra-environmental coupling

We examine the effects of intra-environmental coupling on decoherence by constructing a low temperature spin--spin-bath model of an atomic impurity in a Debye crystal. The impurity interacts with phonons of the crystal through anti-ferromagnetic spin-spin interactions. The reduced density matrix of the central spin representing the impurity is calculated by dynamically integrating the full Schroedinger equation for the spin--spin-bath model for different thermally weighted eigenstates of the spin-bath. Exact numerical results show that increasing the intra-environmental coupling results in suppression of decoherence. This effect could play an important role in the construction of solid state quantum devices such as quantum computers.Comment: 4 pages, 3 figures, Revtex fil

Six-month outcomes from Living Well with Diabetes: a randomized trial of a telephone-delivered weight loss and physical activity intervention to improve glycemic control

Intensive lifestyle intervention trials in type 2 diabetes contribute evidence on what can be achieved under optimal conditions, but are less informative for translation in applied settings

What can the language of musicians tell us about music interaction design?

It is difficult to create good interaction designs for music software or to substantially improve existing designs. One reason is that music involves diverse and complex concepts, entities, relationships, processes, terminologies, and notations. An open challenge for interaction designers is to find systematic ways of channeling the tacit, specialized knowledge of musicians into designs for intuitive user interfaces that can capably support musically skilled users, without excluding those with less technical musical knowledge. One promising new approach to this challenge involves the application of research from the theory of image schemas and conceptual metaphors This theory has already been applied with some success to analyzing musical concepts, mathematical concepts, general-purpose user-interface design, and sound-generation interaction designs involving simple musical parameters such as tempo, volume, and pitch. We propose that by identifying the conceptual metaphors and image schemas used by musical experts when analyzing an excerpt of music, and then assessing the extent to which these conceptual metaphors are supported by existing music-interaction designs, it is possible to identify areas where the designs do not match musicians’ understanding of the domain concepts. This process provides a principled basis for identifying points at which designs could be improved to better support musicians’ understanding and tasks, and for the provisional identification of possible improvements. We present the results of an analysis of dialogue between three musicians as they discuss an excerpt of music. A methodology for the systematic identification of image schemas and conceptual metaphors is detailed. We report on the use of the results of the dialogue analysis to illuminate the designs of two contrasting examples of music software. Areas in which the designs might be made more intuitive are identified, and corresponding suggestions for improvements are outlined. The present research appears to be the first to investigate the potential of conceptual metaphor theory for investigating and improving music-interaction designs that deal with complex musical concepts, such as harmonic progressions, modulation, and voice leading

Monkey Steering Responses Reveal Rapid Visual-Motor Feedback

The neural mechanisms underlying primate locomotion are largely unknown. While behavioral and theoretical work has provided a number of ideas of how navigation is controlled, progress will require direct physiolgical tests of the underlying mechanisms. In turn, this will require development of appropriate animal models. We trained three monkeys to track a moving visual target in a simple virtual environment, using a joystick to control their direction. The monkeys learned to quickly and accurately turn to the target, and their steering behavior was quite stereotyped and reliable. Monkeys typically responded to abrupt steps of target direction with a biphasic steering movement, exhibiting modest but transient overshoot. Response latencies averaged approximately 300 ms, and monkeys were typically back on target after about 1 s. We also exploited the variability of responses about the mean to explore the time-course of correlation between target direction and steering response. This analysis revealed a broad peak of correlation spanning approximately 400 ms in the recent past, during which steering errors provoke a compensatory response. This suggests a continuous, visual-motor loop controls steering behavior, even during the epoch surrounding transient inputs. Many results from the human literature also suggest that steering is controlled by such a closed loop. The similarity of our results to those in humans suggests the monkey is a very good animal model for human visually guided steering

Biallelic GINS2 variant p.(Arg114Leu) causes Meier-Gorlin syndrome with craniosynostosis

Contains fulltext : 284813.pdf (Publisher’s version ) (Open Access)INTRODUCTION: Replication of the nuclear genome is an essential step for cell division. Pathogenic variants in genes coding for highly conserved components of the DNA replication machinery cause Meier-Gorlin syndrome (MGORS). OBJECTIVE: Identification of novel genes associated with MGORS. METHODS: Exome sequencing was performed to investigate the genotype of an individual presenting with prenatal and postnatal growth restriction, a craniofacial gestalt of MGORS and coronal craniosynostosis. The analysis of the candidate variants employed bioinformatic tools, in silico structural protein analysis and modelling in budding yeast. RESULTS: A novel homozygous missense variant NM_016095.2:c.341G>T, p.(Arg114Leu), in GINS2 was identified. Both non-consanguineous healthy parents carried this variant. Bioinformatic analysis supports its classification as pathogenic. Functional analyses using yeast showed that this variant increases sensitivity to nicotinamide, a compound that interferes with DNA replication processes. The phylogenetically highly conserved residue p.Arg114 localises at the docking site of CDC45 and MCM5 at GINS2. Moreover, the missense change possibly disrupts the effective interaction between the GINS complex and CDC45, which is necessary for the CMG helicase complex (Cdc45/MCM2-7/GINS) to accurately operate. Interestingly, our patient's phenotype is strikingly similar to the phenotype of patients with CDC45-related MGORS, particularly those with craniosynostosis, mild short stature and patellar hypoplasia. CONCLUSION: GINS2 is a new disease-associated gene, expanding the genetic aetiology of MGORS

Nonparametric statistical downscaling for the fusion of data of different spatiotemporal support

Statistical downscaling has been developed for the fusion of data of different spatial support. However, environmental data often have different temporal support, which must also be accounted for. This paper presents a novel method of nonparametric statistical downscaling, which enables the fusion of data of different spatiotemporal support through treating the data at each location as observations of smooth functions over time. This is incorporated within a Bayesian hierarchical model with smoothly spatially varying coefficients, which provides predictions at any location or time, with associated estimates of uncertainty. The method is motivated by an application for the fusion of in situ and satellite remote sensing log(chlorophyll-a) data from Lake Balaton, in order to improve the understanding of water quality patterns over space and time

New Insights into Mutable Collagenous Tissue: Correlations between the Microstructure and Mechanical State of a Sea-Urchin Ligament

The mutable collagenous tissue (MCT) of echinoderms has the ability to undergo rapid and reversible changes in passive mechanical properties that are initiated and modulated by the nervous system. Since the mechanism of MCT mutability is poorly understood, the aim of this work was to provide a detailed morphological analysis of a typical mutable collagenous structure in its different mechanical states. The model studied was the compass depressor ligament (CDL) of a sea urchin (Paracentrotus lividus), which was characterized in different functional states mimicking MCT mutability. Transmission electron microscopy, histochemistry, cryo-scanning electron microscopy, focused ion beam/scanning electron microscopy, and field emission gun-environmental scanning electron microscopy were used to visualize CDLs at the micro- and nano-scales. This investigation has revealed previously unreported differences in both extracellular and cellular constituents, expanding the current knowledge of the relationship between the organization of the CDL and its mechanical state. Scanning electron microscopies in particular provided a three-dimensional overview of CDL architecture at the micro- and nano-scales, and clarified the micro-organization of the ECM components that are involved in mutability. Further evidence that the juxtaligamental cells are the effectors of these changes in mechanical properties was provided by a correlation between their cytology and the tensile state of the CDLs