Additional file 2: Figure S1. of Endemic carbapenem-nonsusceptible Acinetobacter baumannii-calcoaceticus complex in intensive care units of the national referral hospital in Jakarta, Indonesia

Carbapenem-nonsusceptible Acinetobacter baumannii-calcoaceticus complex carriage of included patients admitted to adult and ER-ICUs of Dr. Cipto Mangunkusomo General Hospital, Jakarta, Indonesia. (TIFF 1522 kb

Additional file 3: Figure S2. of Endemic carbapenem-nonsusceptible Acinetobacter baumannii-calcoaceticus complex in intensive care units of the national referral hospital in Jakarta, Indonesia

Raman spectroscopy-based cluster analysis of Acinetobacter baumannii-calcoaceticus complex isolates from adult and ER-ICUs. Note: Raman spectra correlation matrix of carbapenem-nonsusceptible A. baumannii-calcoaceticus complex isolates. Isolates are shown in a color-scale (red-orange-yellow-grey) based on their similarity of correlation coefficient value. Red clusters (91–100%) indicate isolates that are indistinguishable according to the cut-off value. Grey areas (≤70%) indicate isolates that are not related. The potentially related isolates are shown by yellow areas (lower similarities (71–80%)) and orange areas (higher similarities (81–90%)). (JPEG 761 kb

Стихотворенiя K. P. : op. 20 / мyзыка A. Aлфераки

Comprend : 1 - Повѣяло черемухой (5 p.) - 3 - Лира (5 p.) - 4 - Прощание с Неаполем (3 p.) - 5 - Царь Саул (7 p.

Typing <i>Pseudomonas aeruginosa</i> Isolates with Ultrahigh Resolution MALDI-FTICR Mass Spectrometry

The introduction of standardized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) platforms in the medical microbiological practice has revolutionized the way microbial species identification is performed on a daily basis. To a large extent, this is due to the ease of operation. Acquired spectra are compared to profiles obtained from cultured colonies present in a reference spectra database. It is fast and reliable, and costs are low compared to previous diagnostic approaches. However, the low resolution and dynamic range of the MALDI-TOF profiles have shown limited applicability for the discrimination of different bacterial strains, as achieved with typing based on genetic markers. This is pivotal in cases where certain strains are associated with, e.g., virulence or antibiotic resistance. Ultrahigh resolution MALDI-FTICR MS allows the measurement of small proteins at isotopic resolution and can be used to analyze complex mixtures with increased dynamic range and higher precision than MALDI-TOF MS, while still generating results in a similar time frame. Here, we propose to use ultrahigh resolution 15T MALDI-Fourier transform ion cyclotron resonance (FTICR) MS to discriminate clinically relevant bacterial strains after species identification performed by MALDI-TOF MS. We used a collection of well characterized <i>Pseudomonas aeruginosa</i> strains, featuring distinct antibiotic resistance profiles, and isolates obtained during hospital outbreaks. Following cluster analysis based on amplification fragment length polymorphism (AFLP), these strains were grouped into three different clusters. The same clusters were obtained using protein profiles generated by MALDI-FTICR MS. Subsequent intact protein analysis by electrospray ionization (ESI)-collision-induced dissociation (CID)-FTICR MS was applied to identify protein isoforms that contribute to the separation of the different clusters, illustrating the additional advantage of this analytical platform

Pintavedenoton suhteen kriittisimmät väyläosuudet liikenteen ja väylänpidon kannalta

<i>N</i>-Leucinyl benzenesulfonamides have been discovered as a novel class of potent inhibitors of <i>E. coli</i> leucyl-tRNA synthetase. The binding of inhibitors to the enzyme was measured by using isothermal titration calorimetry. This provided information on enthalpy and entropy contributions to binding, which, together with docking studies, were used for structure–activity relationship analysis. Enzymatic assays revealed that <i>N</i>-leucinyl benzenesulfonamides display remarkable selectivity for <i>E. coli</i> leucyl-tRNA synthetase compared to <i>S. aureus</i> and human orthologues. The simplest analogue of the series, <i>N</i>-leucinyl benzenesulfonamide (R = H), showed the highest affinity against <i>E. coli</i> leucyl-tRNA synthetase and also exhibited antibacterial activity against Gram-negative pathogens (the best MIC = 8 μg/mL, <i>E. coli</i> ATCC 25922), which renders it as a promising template for antibacterial drug discovery