Drug interference in Trinder reaction

Our recent research of drug interference in Trinder reaction – a widely used chromogenic reaction in the field of clinical biochemistry, was focused on comparison of various drugs and the impact on determination of several biochemical parameters with validated diagnostic kits utilizing Trinder reaction. Our results showed significant alteration of results in therapeutic concentrations of ACC®, Dicynone® and Novalgin® in comparison to the control samples. Dobutamine, Tensamin and ascorbic acid showed significant interference only in samples containing 1 mmol/l of drug.V našem posledním výzkumu lékové interference v Trinderově reakci, široce rozšířené chromogenní reakci v oboru klinické biochemie, jsme se zaměřili na porovnání různých leků a jejich dopad na měření několika biochemických paramerů pomocí validovaných diagnostických souprav využívající Trinderovu reakci. Naše výsledky ukázaly významnou změnu výsledků při terapeutických koncentracích ACC, Dicynonu a Novalginu ve srovnání s kontrolními vzorky. Dobutamin, Tensamin a kyselina askorbová projevovaly významnou interferenci až u vzorků obsahujícíc koncentraci léku 1 mmol/l

Interference Dicynone v Trinderově reakci – porovnání produktů dodavatelů IVD

V této části našeho výzkumu jsme se zaměřili na porovnání interference Trinderovy reakce lékem Dicynone, u reagenčních setů různých výrobců in vitro diagnostiky působící v České republice. Trinderova reakce je v klinické biochemii poměrně rozšířená chromogenní reakce a Dicynone je široce užívané hemostatikum. Na výsledcích ukazujeme jednak to, že žádný z výrobců tuto interferenci zatím spolehlivě nevyřešil, ale také to, že se dá do značné míry omezit, jak lze uvést na příkladu fy Erba Lachema.This section of our Dicynone interference in Trinder reaction research was focused on performance of different in vitro diagnostic providers and their reagent sets from located in Czech Republic. Trinder reaction is a widely used chromogenic reaction and Dicynone is a abundantly used hemostatic medication. The results show that the interference is universal in all reagent kits utilizing Trinder reaction and also that the interference effect could be reduced as is shown in Erba Lachema company results

Video-Based Human-Computer Interface

This bachelor's thesis descripts a way of controlling a computer by hand gestures. Picture is taken from web camera, in which search for a position of hand in picture and recognize gesture. The gesture signal controls a computer. I use the OpenCV library for a work with picture. At the end are output pictures

Natural and historic heritage of the Bochnia Salt Mine (South Poland)

The Bochnia Salt Mine, presented in this paper, is situated ca. 40 km E of Cracow, in the southern part of the Neogene Carpathian Foredeep, close to the Carpathian edge. In this region the rock-salt deposits formed as a result of the Late Miocene folding and local tectonic thickening of Badenian evaporites. The Bochnia deposit, situated in the almost vertical N limb of the Bochnia Anticline, stretches ca. 7 km WE, but only 15-200 m NS. Salt mining in Bochnia began in the thirteenth c. and continued until 1990. The historic part of the mine, since 1995 operated by a company supplying health and tourism services, is an officially listed monument of historic heritage. Legal protection also comprises 27 sites of key value for the geology of the deposit. These documentation sites record the whole profile of the evaporite series and the adjacent beds, main and minor tectonic structures, as well as mineralogical curiosities, such as fibrous halite and enterolithic anhydrite. For some years, efforts have been made to enter the Bochnia Salt Mine, in 2008 visited by over 140 000 tourists, on the UNESCO World List of Cultural and Natural Heritage

Evaluation of FUS-2000 urine analyzer: analytical properties and particle recognition

<p>This study evaluates the performance of microscopic part of a hybrid analyzer FUS-2000 (Dirui Industrial Co., Changchun, China), its analytical properties and particle recognition. The evaluation of trueness, repeatability, detection limit, carry-over, linearity range and analytical stability was performed according to Dirui protocol guidelines designed by Dirui Company to guarantee the quality of the instrument. Trueness for low, medium and high-value concentrations was calculated with bias of 15.5, 4.7 and −6.6%, respectively. Detection limit of 5 Ery/μl was confirmed. Coefficient of variation of 11.0, 5.2 and 3.8% was measured for within-run repeatability of low, medium and high concentration. Between-run repeatability for daily quality control had coefficient of variation of 3.0%. Carry-over did not exceed 0.05%. Linearity was confirmed for range of 0–16,000 particles/μl (<i>R</i>² = 0.9997). The analytical stability had coefficient of variation of 4.3%. Out of 1258 analyzed urine samples, 362 positive were subjected to light microscopy urine sediment analysis and compared to the analyzer results. Cohen’s kappa coefficients were calculated to express the concordance. Squared kappa coefficient was 0.927 (red blood cells), 0.888 (white blood cells), 0.908 (squamous epithelia), 0.634 (transitional epithelia), 0.628 (hyaline casts), 0.843 (granular casts) and 0.623 (bacteria). Single kappa coefficients were 0.885 (yeasts) and 0.756 (crystals), respectively. Aforementioned results show good analytical performance of the analyzer and tight agreement with light microscopy of urine sediment.</p