Genotyping of Giardia in Dutch patients and animals: a phylogenetic analysis of human and animal isolates.

Giardia duodenalis (syn. Giardia lamblia, Giardia intestinalis) is a protozoan organism that can infect the intestinal tract of many animal species including mammals. Genetic heterogeneity of G. duodenalis is well described but the zoonotic potential is still not clear. In this study, we analysed 100 Giardia DNA samples directly isolated from human stool specimens, to get more insight in the different G. duodenalis assemblages present in the Dutch human population. Results showed that these human isolates could be divided into two main Assemblages A and B within the G. duodenalis group on the basis of PCR assays specific for the Assemblages A and B and the DNA sequences of 18S ribosomal RNA and the glutamate dehydrogenase (gdh) genes. Genotyping results showed that G. duodenalis isolates originating from Dutch human patients belonged in 35% of the cases to Assemblage A (34/98) and in 65% of the cases to Assemblage B (64/98) whereas two human cases remained negative in all assays tested. In addition, we compared these human samples with animal samples from the Netherlands and human and animal samples from other countries. A phylogenetic analysis was carried out on the DNA sequences obtained from these Giardia and those available in GenBank. Using gdh DNA sequence analysis, human and animal Assemblage A and B Giardia isolates could be identified. However, phylogenetic analysis revealed different sub-clustering for human and animal isolates where host-species-specific assemblages (C, D, E, F and G) could be identified. The geographic origin of the human and animal samples was not a discriminating factor

Multipartite entangled coherent states

We propose a scheme for generating multipartite entangled coherent states via entanglement swapping, with an example of a physical realization in ion traps. Bipartite entanglement of these multipartite states is quantified by the concurrence. We also use the $N$--tangle to compute multipartite entanglement for certain systems. Finally we establish that these results for entanglement can be applied to more general multipartite entangled nonorthogonal states.Comment: 7 pages, two figures. We added more detail discussions on the generation of multipartite entangled coherent states and multipartite entangelemen

Quantum nonlocality and applications in quantum-information processing of hybrid entangled states

The hybrid entangled states generated, e.g., in a trapped-ion or atom-cavity system, have exactly one ebit of entanglement, but are not maximally entangled. We demonstrate this by showing that they violate, but in general do not maximally violate, Bell's inequality due to Clauser, Horne, Shimony and Holt. These states are interesting in that they exhibit the entanglement between two distinct degrees of freedom (one is discrete and another is continuous). We then demonstrate these entangled states as a valuable resource in quantum information processing including quantum teleportation, entanglement swapping and quantum computation with "parity qubits". Our work establishes an interesting link between quantum information protocols of discrete and continuous variables.Comment: 5 pages, no figur

Nonclassical Fields and the Nonlinear Interferometer

We demonstrate several new results for the nonlinear interferometer, which emerge from a formalism which describes in an elegant way the output field of the nonlinear interferometer as two-mode entangled coherent states. We clarify the relationship between squeezing and entangled coherent states, since a weak nonlinear evolution produces a squeezed output, while a strong nonlinear evolution produces a two-mode, two-state entangled coherent state. In between these two extremes exist superpositions of two-mode coherent states manifesting varying degrees of entanglement for arbitrary values of the nonlinearity. The cardinality of the basis set of the entangled coherent states is finite when the ratio $\chi / \pi$ is rational, where $\chi$ is the nonlinear strength. We also show that entangled coherent states can be produced from product coherent states via a nonlinear medium without the need for the interferometric configuration. This provides an important experimental simplification in the process of creating entangled coherent states.Comment: 21 pages, 2 figure

Chaos and coherence in an optical system subject to photon nondemolition measurement

We consider the effect of quantum-nondemolition monitoring of the photon number on the quantum suppression of classical chaos in a recently proposed quantum optical model, the parametrically kicked nonlinear oscillator [G. J. Milburn, Phys. Rev. A 41, 6567 (1990)]. Classically the effect of the quantum-nondemolition measurement is equivalent to a phase diffusion in the phase plane of the oscillator. A similar result holds in the quantum description, but in addition the measurement rapidly diagonalizes the system density operator in the photon-number basis. This has the effect of causing the evolution of the quantum moments to approach the corresponding classical moments and thus restores the classical dynamics for practical purposes