2,517 research outputs found

    What Science Contributes to Agriculture

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    Image interpolation using Shearlet based iterative refinement

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    This paper proposes an image interpolation algorithm exploiting sparse representation for natural images. It involves three main steps: (a) obtaining an initial estimate of the high resolution image using linear methods like FIR filtering, (b) promoting sparsity in a selected dictionary through iterative thresholding, and (c) extracting high frequency information from the approximation to refine the initial estimate. For the sparse modeling, a shearlet dictionary is chosen to yield a multiscale directional representation. The proposed algorithm is compared to several state-of-the-art methods to assess its objective as well as subjective performance. Compared to the cubic spline interpolation method, an average PSNR gain of around 0.8 dB is observed over a dataset of 200 images

    Transmon in a semi-infinite high-impedance transmission line: Appearance of cavity modes and Rabi oscillations

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    In this paper, we investigate the dynamics of a single superconducting artificial atom capacitively coupled to a transmission line with a characteristic impedance comparable to or larger than the quantum resistance. In this regime, microwaves are reflected from the atom also at frequencies far from the atom\u27s transition frequency. Adding a single mirror in the transmission line then creates cavity modes between the atom and the mirror. Investigating the spontaneous emission from the atom, we then find Rabi oscillations, where the energy oscillates between the atom and one of the cavity modes

    Semiclassical analysis of dark-state transient dynamics in waveguide circuit QED

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    The interaction between superconducting qubits and one-dimensional microwave transmission lines has been studied experimentally and theoretically in the past two decades. In this work, we investigate the spontaneous emission of an initially excited artificial atom which is capacitively coupled to a semi-infinite transmission line, shorted at one end. This configuration can be viewed as an atom in front of a mirror. The distance between the atom and the mirror introduces a time delay in the system, which we take into account fully. When the delay time equals an integer number of atom oscillation periods, the atom converges into a dark state after an initial decay period. The dark state is an effect of destructive interference between the reflected part of the field and the part directly emitted by the atom. Based on circuit quantization, we derive linearized equations of motion for the system and use these for a semiclassical analysis of the transient dynamics. We also make a rigorous connection to the quantum optics system-reservoir approach and compare these two methods to describe the dynamics. We find that both approaches are equivalent for transmission lines with a low characteristic impedance, while they differ when this impedance is higher than the typical impedance of the superconducting artificial atom

    Methods of editing cloud and atmospheric layer affected pixels from satellite data

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    The location and migration of cloud, land and water features were examined in spectral space (reflective VIS vs. emissive IR). Daytime HCMM data showed two distinct types of cloud affected pixels in the south Texas test area. High altitude cirrus and/or cirrostratus and "subvisible cirrus" (SCi) reflected the same or only slightly more than land features. In the emissive band, the digital counts ranged from 1 to over 75 and overlapped land features. Pixels consisting of cumulus clouds, or of mixed cumulus and landscape, clustered in a different area of spectral space than the high altitude cloud pixels. Cumulus affected pixels were more reflective than land and water pixels. In August the high altitude clouds and SCi were more emissive than similar clouds were in July. Four-channel TIROS-N data were examined with the objective of developing a multispectral screening technique for removing SCi contaminated data

    Accumulation of formamide in hydrothermal pores to form prebiotic nucleobases

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    Formamide is one of the important compounds from which prebiotic molecules can be synthesized, provided that its concentration is sufficiently high. For nucleotides and short DNA strands, it has been shown that a high degree of accumulation in hydrothermal pores occurs, so that temperature gradients might play a role in the origin of life [Baaske P, et al. (2007) Proc Natl Acad Sci USA 104(22):9346−9351]. We show that the same combination of thermophoresis and convection in hydrothermal pores leads to accumulation of formamide up to concentrations where nucleobases are formed. The thermophoretic properties of aqueous formamide solutions are studied by means of Infrared Thermal Diffusion Forced Rayleigh Scattering. These data are used in numerical finite element calculations in hydrothermal pores for various initial concentrations, ambient temperatures, and pore sizes. The high degree of formamide accumulation is due to an unusual temperature and concentration dependence of the thermophoretic behavior of formamide. The accumulation fold in part of the pores increases strongly with increasing aspect ratio of the pores, and saturates to highly concentrated aqueous formamide solutions of ∼85 wt% at large aspect ratios. Time-dependent studies show that these high concentrations are reached after 45–90 d, starting with an initial formamide weight fraction of 10−3 wt % that is typical for concentrations in shallow lakes on early Earth

    Methods for expression of recombinant proteins using a Pichia pastoris cell-free system

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    Cell‐free protein synthesis is a powerful tool for engineering biology and has been utilized in many diverse applications, from biosensing and protein prototyping to biomanufacturing and the design of metabolic pathways. By exploiting host cellular machinery decoupled from cellular growth, proteins can be produced in vitro both on demand and rapidly. Eukaryotic cell‐free platforms are often neglected due to perceived complexity and low yields relative to their prokaryotic counterparts, despite providing a number of advantageous properties. The yeast Pichia pastoris (also known as Komagataella phaffii) is a particularly attractive eukaryotic host from which to generate cell‐free extracts, due to its ability to grow to high cell densities with high volumetric productivity, genetic tractability for strain engineering, and ability to perform post‐translational modifications. Here, we describe methods for conducting cell‐free protein synthesis using P. pastoris as the host, from preparing the cell lysates to protocols for both coupled and linked transcription‐translation reactions. By providing these methodologies, we hope to encourage the adoption of the platform by new and experienced users alike

    Identifying seizure onset zone and epileptic networks with the dynamic imaging of coherent sources [Abstract]

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    Purpose: In the presurgical evaluation of children with refractory focalepilepsy the main difficulty is to locate the exact point of seizure onset.The aim of this study was to characterize the areas of seizure onset as wellas the epileptic network involved in seizure propagation usingDynamicimaging of coherent sources (DICS)of ictal EEGs. Method: DICS is an inverse solution in the frequency domain whichdescribes neuronal networks and coherence of oscillatory brain activityby applying a spatial filter (Gross et al. PNAS 2001; 98:694–699). In 15children with refractory focal epilepsy, typical seizures were selectedfrom the EEGs recorded during the presurgical evaluation. For every sei-zure, two data sets of 10 s duration were extracted: one EEG segmentcontained the seizure onset and the other segment included the middlepart of the seizure. For both segments, the frequency range was definedand analyzed with DICS. The brain area with the strongest power in thecorresponding frequency range was defined as a reference region and itscoherence with the entire brain was computed using DICS. The result ofthe reference region was compared with the electroclinical localizationof seizure onset as well as with the postoperative resection site to deter-mine concordance. Results: For the beginning of the seizure, a good concordance betweenresults of the DICS localization and postoperative outcome was achievedin all 15 patients. The analysis of seizure propagation revealed an epilep-tic network which resembled reverberation of epileptic activity betweendifferent brain areas. Conclusion: DICS may be a useful tool to define the seizure onset zoneand study epileptic networks
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