Long-Term Outcome after Joint Bleeds in Von Willebrand Disease Compared to Haemophilia A: A Post Hoc Analysis

Thrombosis and Hemostasi

Resistance to GSH depletion maps to the interface between 2 protomers.

<p>(<b>A</b>) Surface rendered view of a single pentameric subunit (colored) in a whole capsid of CVB3 strain M (PDB 1COV). The remaining surface of the virion is colored grey. Viral proteins within a single pentameric unit are colored denoting VP1 (yellow), VP2 (green) and VP3 (aquamarine). A pentameric unit showing the location of the different mutations conferring GSH-insensitivity identified from the revertant screen (red) at the interface of 2 protomers. One protomer within the pentameric structure is outlined and an enlarged view on the antiparallel β-sheets in VP1 (colored yellow) and the location of the identified amino acids (colored red) on the interface between two protomers is provided as well. Comparison of pentamers of various GSH-insensitive (<b>B</b>) [echo11 (1h8t) and EV71 (4aed)] and GSH-sensitive (<b>C</b>) [CVB3 (1cov), echo 1 (1ev1), CVA21 (1z7s) and RV14 (1ro8)] enteroviruses. Surface representation of different protomers within a pentamer. Surface exposed methionines are indicated in red. An enlarged view on the location of the identified surface exposed methionines (colored red) on the interface between two protomers is also provided. Molecular graphics and analyses were performed with the UCSF Chimera package. Chimera is developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco (supported by NIGMS P41-GM103311) <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004039#ppat.1004039-Pettersen1" target="_blank">[58]</a>.</p