Fictions of organized crime: introduction

Introduction to the themed issue of New Readings

Introduction of a non-ported peripheral intravenous catheter with multi-use blood control septum offers improvements in the overall efficiency of the procedure and is clinically well accepted

Financial challenges that the National Health Service (NHS), England, faces may jeopardise its future. This study evaluated the direct cost of using two different safety peripheral intravenous cannulae (SPIVC) with and without a blood control septum, including the cost of device and clinician time. Observation of 103 cannulations demonstrated a 54 second (29%) time reduction per cannulation with the non-ported SPIVC with multi-use blood control septum (Introcan Safety® 3 B Braun), compared to the standard ported SPIVC (Vasofix® Safety B Braun) (P<0.05). The direct cost analysis, including clinician time, demonstrated that the introduction of SPIVC with multi-use blood control septum could offer time efficiency savings equivalent to a reduction in average cannulation costs by 25%. Eighty two per cent of users perceived the insertion of SPIVC with multi-use blood control septum to be easy to use; 82% would choose to use it in clinical practice

Love & law: The aura of prison writing in Mexico, from the 1800s to the present

Prison writing has largely been excluded from the literary canon of the Spanish-speaking world, even though it encompasses key names that extend as far back as Cervantes in Spain and Lizardi in Mexico: two of the pioneers of what is now called the “novel”. Building on seminal analyses of Latin American literature by Roberto González Echevarría, Ángel Rama and Doris Sommer, this article addresses the following questions: What is the power of prison writing? How might we interpret its status as a genre, both in historical and contemporary terms? And what do contemporary forms of prison writing share with much older examples? To answer these questions, we analyze prison narratives from the nineteenth, twentieth, and twenty-first centuries, including well-known novels by José Joaquín Fernández de Lizardi and José Revueltas, and narratives by emerging writers from Susuki Lee, Águila del Mar, and Amatista Lee to Julio Grotten. Through an “auratic quality” that, we argue, derives from their ability to develop powerful counter-truths through the experience of confinement, these narratives reveal and resist the subjugation of the subject by the state as the latter intervenes violently in politics and private life. Our contention is that the power of prison writing lies in its ability to turn legal, state-sponsored discourse on its head through the production of alternative stories narrated from within prison and from below; and that these are simultaneously founded on legal discourse and its affective underside; on law and love

The Role of Basin Geometry in Mountain Snowpack Responses to Climate Change

This article was submitted to Water and Hydrocomplexity, a section of the journal Frontiers in WaterCanada First Research Excellence Fund’s Global Water Futures program, the Canada Research Chairs and Canada Excellence Research Chairs programs, the Natural Sciences and Engineering Research Council of Canada (NSERC Discovery Grants to JS and JP) and the Alberta Innovates Water Innovation ProgramPeer ReviewedSnowmelt contributions to streamflow in mid-latitude mountain basins typically dominate other runoff sources on annual and seasonal timescales. Future increases in temperature and changes in precipitation will affect both snow accumulation and seasonal runoff timing and magnitude, but the underlying and fundamental roles of mountain basin geometry and hypsometry on snowmelt sensitivity have received little attention. To investigate the role of basin geometry in snowmelt sensitivity, a linear snow accumulation model and the Cold Regions Hydrological Modeling (CRHM) platform driven are used to estimate how hypsometry affects basin-wide snow volumes and snowmelt runoff. Area-elevation distributions for fifty basins in western Canada were extracted, normalized according to their elevation statistics, and classified into three clusters that represent top-heavy, middle, and bottom-heavy basins. Prescribed changes in air temperature alter both the snow accumulation gradient and the total snowmelt energy, leading to snowpack volume reductions (10–40%), earlier melt onsets (1–4 weeks) and end of melt season (3 weeks), increases in early spring melt rates and reductions in seasonal areal melt rates (up to 50%). Basin hypsometry controls the magnitude of the basin response. The most sensitive basins are bottom-heavy, and have a greater proportion of their area at low elevations. The least sensitive basins are top-heavy, and have a greater proportion of their area at high elevations. Basins with similar proportional areas at high and low elevations fall in between the others in terms of sensitivity and other metrics. This work provides context for anticipating the impacts of ongoing hydrological change due to climate change, and provides guidance for both monitoring networks and distributed modeling efforts

Capsular profiling of the Cronobacter genus and the association of specific Cronobacter sakazakii and C. malonaticus capsule types with neonatal meningitis and necrotizing enterocolitis

Background: Cronobacter sakazakii and C. malonaticus can cause serious diseases especially in infants where they are associated with rare but fatal neonatal infections such as meningitis and necrotising enterocolitis. Methods: This study used 104 whole genome sequenced strains, covering all seven species in the genus, to analyse capsule associated clusters of genes involved in the biosynthesis of the O-antigen, colanic acid, bacterial cellulose, enterobacterial common antigen (ECA), and a previously uncharacterised K-antigen. Results: Phylogeny of the gnd and galF genes flanking the O-antigen region enabled the defining of 38 subgroups which are potential serotypes. Two variants of the colanic acid synthesis gene cluster (CA1 and CA2) were found which differed with the absence of galE in CA2. Cellulose (bcs genes) were present in all species, but were absent in C. sakazakii sequence type (ST) 13 and clonal complex (CC) 100 strains. The ECA locus was found in all strains. The K-antigen capsular polysaccharide Region 1 (kpsEDCS) and Region 3 (kpsMT) genes were found in all Cronobacter strains. The highly variable Region 2 genes were assigned to 2 homology groups (K1 and K2). C. sakazakii and C. malonaticus isolates with capsular type [K2:CA2:Cell+] were associated with neonatal meningitis and necrotizing enterocolitis. Other capsular types were less associated with clinical infections. Conclusion: This study proposes a new capsular typing scheme which identifies a possible important virulence trait associated with severe neonatal infections. The various capsular polysaccharide structures warrant further investigation as they could be relevant to macrophage survival, desiccation resistance, environmental survival, and biofilm formation in the hospital environment, including neonatal enteral feeding tubes

Targeted Metabolomics Analysis of Campylobacter coli VC167 Reveals Legionaminic Acid Derivatives as Novel Flagellar Glycans

Glycosylation of Campylobacter flagellin is required for the biogenesis of a functional flagella filament. Recently, we used a targeted metabolomics approach using mass spectrometry and NMR to identify changes in the metabolic profile of wild type and mutants in the flagellar glycosylation locus, characterize novel metabolites, and assign function to genes to define the pseudaminic acid biosynthetic pathway in Campylobacter jejuni 81-176 (McNally, D. J., Hui, J. P., Aubry, A. J., Mui, K. K., Guerry, P., Brisson, J. R., Logan, S. M., and Soo, E. C. (2006) J. Biol. Chem. 281, 18489-18498). In this study, we use a similar approach to further define the glycome and metabolomic complement of nucleotide-activated sugars in Campylobacter coli VC167. Herein we demonstrate that, in addition to CMP-pseudaminic acid, C. coli VC167 also produces two structurally distinct nucleotide-activated nonulosonate sugars that were observed as negative ions at m/z 637 and m/z 651 (CMP-315 and CMP-329). Hydrophilic interaction liquid chromatography-mass spectrometry yielded suitable amounts of the pure sugar nucleotides for NMR spectroscopy using a cold probe. Structural analysis in conjunction with molecular modeling identified the sugar moieties as acetamidino and N-methylacetimidoyl derivatives of legionaminic acid (Leg5Am7Ac and Leg5AmNMe7Ac). Targeted metabolomic analyses of isogenic mutants established a role for the ptmA-F genes and defined two new ptm genes in this locus as legionaminic acid biosynthetic enzymes. This is the first report of legionaminic acid in Campylobacter sp. and the first report of legionaminic acid derivatives as modifications on a protein

Altered Intrinsic Functional Brain Architecture in Children at Familial Risk of Major Depression

Background Neuroimaging studies of patients with major depression have revealed abnormal intrinsic functional connectivity measured during the resting state in multiple distributed networks. However, it is unclear whether these findings reflect the state of major depression or reflect trait neurobiological underpinnings of risk for major depression. Methods We compared resting-state functional connectivity, measured with functional magnetic resonance imaging, between unaffected children of parents who had documented histories of major depression (at-risk, n = 27; 8–14 years of age) and age-matched children of parents with no lifetime history of depression (control subjects, n = 16). Results At-risk children exhibited hyperconnectivity between the default mode network and subgenual anterior cingulate cortex/orbital frontal cortex, and the magnitude of connectivity positively correlated with individual symptom scores. At-risk children also exhibited 1) hypoconnectivity within the cognitive control network, which also lacked the typical anticorrelation with the default mode network; 2) hypoconnectivity between left dorsolateral prefrontal cortex and subgenual anterior cingulate cortex; and 3) hyperconnectivity between the right amygdala and right inferior frontal gyrus, a key region for top-down modulation of emotion. Classification between at-risk children and control subjects based on resting-state connectivity yielded high accuracy with high sensitivity and specificity that was superior to clinical rating scales. Conclusions Children at familial risk for depression exhibited atypical functional connectivity in the default mode, cognitive control, and affective networks. Such task-independent functional brain measures of risk for depression in children could be used to promote early intervention to reduce the likelihood of developing depression

Pilot Trial on the Use of Etanercept and Methylprednisolone as Primary Treatment for Acute Graft-versus-Host Disease

AbstractClinical and preclinical data indicate that tumor necrosis factor (TNF)–α is an important mediator of acute graft-versus-host disease (aGVHD) after allogeneic bone marrow transplantation. We completed a study using etanercept, a fusion protein capable of neutralizing TNF-α, for the initial treatment of aGVHD. Etanercept (25 mg subcutaneously) was administered twice weekly for 16 doses, along with methylprednisolone (2 mg/kg) and tacrolimus for biopsy-proven aGVHD. Twenty patients with a median age of 47 years (range, 8–63 years) were enrolled. Fourteen patients with grade II aGVHD (11 family donors and 3 unrelated donors) and 6 patients with grade III aGVHD (3 family donors and 3 unrelated donors) were treated. Twelve patients completed 16 doses of therapy, and 8 received 5 to 15 doses. Reasons for not completing all doses of etanercept included progression of aGVHD (n = 4), relapsed leukemia (n = 2), progression of pulmonary and central nervous system lesions (n = 1), and perforated duodenal ulcer (n = 1). Fifteen (75%) of 20 patients had complete resolution of aGVHD within 4 weeks of therapy. Increasing levels of soluble TNF receptor 1 plasma concentration during the first 4 weeks of therapy indicated progression of aGVHD in 5 patients. In contrast, for 15 responding patients, soluble TNF receptor 1 plasma concentration levels returned to baseline. These data demonstrate the feasibility of using cytokine blockade in the early treatment of aGVHD