287 research outputs found
What\u27s Right with Gavin? Examining Literacy Practices of a Neurodiverse Youth From an Ethics of Care Perspective
Genome evolution and hostâmicrobiome shifts correspond with intraspecific niche divergence within harmful algal bloomâforming Microcystis aeruginosa
Intraspecific niche divergence is an important driver of species range, population abundance and impacts on ecosystem functions. Genetic changes are the primary focus when studying intraspecific divergence; however, the role of ecological interactions, particularly hostâmicrobiome symbioses, is receiving increased attention. The relative importance of these evolutionary and ecological mechanisms has seen only limited evaluation. To address this question, we used Microcystis aeruginosa, the globally distributed cyanobacterium that dominates freshwater harmful algal blooms. These blooms have been increasing in occurrence and intensity worldwide, causing major economic and ecological damages. We evaluated 46 isolates of M. aeruginosa and their microbiomes, collected from 14 lakes in Michigan, USA, that vary over 20âfold in phosphorus levels, the primary limiting nutrient in freshwater systems. Genomes of M. aeruginosa diverged along this phosphorus gradient in genomic architecture and protein functions. Fitness in lowâphosphorus lakes corresponded with additional shifts within M. aeruginosa including genomeâwide reductions in nitrogen use, an expansion of phosphorus assimilation genes and an alternative life history strategy of nonclonal colony formation. In addition to host shifts, despite culturing in commonâgarden conditions, hostâmicrobiomes diverged along the gradient in taxonomy, but converged in function with evidence of metabolic interdependence between the host and its microbiome. Divergence corresponded with a physiological tradeâoff between fitness in lowâphosphorus environments and growth rate in phosphorusârich conditions. Coâoccurrence of genotypes adapted to different nutrient environments in phosphorusârich lakes may have critical implications for understanding how M. aeruginosa blooms persist after initial nutrient depletion. Ultimately, we demonstrate that the intertwined effects of genome evolution, host life history strategy and ecological interactions between a host and its microbiome correspond with an intraspecific niche shift with important implications for whole ecosystem function.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/151861/1/mec15198_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/151861/2/mec15198.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/151861/3/mec15198-sup-0001-Supinfo.pd
Decreased antiviral immune response within the central nervous system of aged mice is associated with increased lethality of West Nile virus encephalitis
West Nile virus (WNV) is an emerging pathogen that causes disease syndromes ranging from a mild flu-like illness to encephalitis. While the incidence of WNV infection is fairly uniform across age groups, the risk of lethal encephalitis increases with advanced age. Prior studies have demonstrated age-related, functional immune deficits that limit systemic antiviral immunity and increase mortality; however, the effect of age on antiviral immune responses specifically within the central nervous system (CNS) is unknown. Here, we show that aged mice exhibit increased peripheral organ and CNS tissue viral burden, the latter of which is associated with alterations in activation of both myeloid and lymphoid cells compared with similarly infected younger animals. Aged mice exhibit lower MHCII expression by microglia, and higher levels of PD1 and lower levels of IFNÎł expression by WNV-specific CD
Surveys for ticks on wildlife hosts and in the environment at Asian longhorned tick (\u3ci\u3eHaemaphysalis longicornis\u3c/i\u3e)-positive sites in Virginia and New Jersey, 2018
Haemaphysalis longicornis, the Asian longhorned tick (ALT), is native to eastern Asia, but it has become invasive in several countries, including Australia, New Zealand and recently in the eastern United States (US). To identify wild mammal and avian host species in the US, we conducted active wildlife surveillance in two states with known ALT infestations (Virginia and New Jersey). In addition, we conducted environmental surveys in both states. These surveillance efforts resulted in detection of 51 ALTinfested individuals from seven wildlife species, including raccoon (Procyon lotor), Virginia opossum (Didelphis virginiana), red fox (Vulpes vulpes), woodchuck (Marmota monax), eastern cottontail (Sylvilagus floridanus), striped skunk (Mephitis mephitis) and white-tailed deer (Odocoileus virginianus). We found ALT in the environment in both states and also collected three native tick species (Amblyomma americanum, Dermacentor variablis and Ixodes scapularis) that are vectors of pathogens of public health and veterinary importance. This study provides important specific information on the wildlife host range of ALT in the US
Computational Modeling for the Activation Cycle of G-proteins by G-protein-coupled Receptors
In this paper, we survey five different computational modeling methods. For
comparison, we use the activation cycle of G-proteins that regulate cellular
signaling events downstream of G-protein-coupled receptors (GPCRs) as a driving
example. Starting from an existing Ordinary Differential Equations (ODEs)
model, we implement the G-protein cycle in the stochastic Pi-calculus using
SPiM, as Petri-nets using Cell Illustrator, in the Kappa Language using
Cellucidate, and in Bio-PEPA using the Bio-PEPA eclipse plug in. We also
provide a high-level notation to abstract away from communication primitives
that may be unfamiliar to the average biologist, and we show how to translate
high-level programs into stochastic Pi-calculus processes and chemical
reactions.Comment: In Proceedings MeCBIC 2010, arXiv:1011.005
Molecular Characterization of Haemaphysalis Species and a Molecular Genetic Key for the Identification of Haemaphysalis of North America
Haemaphysalis longicornis (Acari: Ixodidae), the Asian longhorned tick, is native to East Asia, but has become established in Australia and New Zealand, and more recently in the United States. In North America, there are other native Haemaphysalis species that share similar morphological characteristics and can be difficult to identify if the specimen is damaged. The goal of this study was to develop a cost-effective and rapid molecular diagnostic assay to differentiate between exotic and native Haemaphysalis species to aid in ongoing surveillance of H. longicornis within the United States and help prevent misidentification. We demonstrated that restriction fragment length polymorphisms (RFLPs) targeting the 16S ribosomal RNA and the cytochrome c oxidase subunit I (COI) can be used to differentiate H. longicornis from the other Haemaphysalis species found in North America. Furthermore, we show that this RFLP assay can be applied to Haemaphysalis species endemic to other regions of the world for the rapid identification of damaged specimens. The work presented in this study can serve as the foundation for region specific PCR-RFLP keys for Haemaphysalis and other tick species and can be further applied to other morphometrically challenging taxa
Period and chemical evolution of SC stars
The SC and CS stars are thermal-pulsing AGB stars with C/O ratio close to
unity. Within this small group, the Mira variable BH Cru recently evolved from
spectral type SC (showing ZrO bands) to CS (showing weak C2). Wavelet analysis
shows that the spectral evolution was accompanied by a dramatic period
increase, from 420 to 540 days, indicating an expanding radius. The pulsation
amplitude also increased. Old photographic plates are used to establish that
the period before 1940 was around 490 days. Chemical models indicate that the
spectral changes were caused by a decrease in stellar temperature, related to
the increasing radius. There is no evidence for a change in C/O ratio. The
evolution in BH Cru is unlikely to be related to an on-going thermal pulse.
Periods of the other SC and CS stars, including nine new periods, are
determined. A second SC star, LX Cyg, also shows evidence for a large increase
in period, and one further star shows a period inconsistent with a previous
determination. Mira periods may be intrinsically unstable for C/O ~ 1; possibly
because of a feedback between the molecular opacities, pulsation amplitude, and
period. LRS spectra of 6 SC stars suggest a feature at wavelength > 15 micron,
which resembles one recently attributed to the iron-sulfide troilite. Chemical
models predict a large abundance of FeS in SC stars, in agreement with the
proposed association.Comment: 14 pages, 20 figures. MNRAS, 2004, accepted for publication. Janet
Mattei, one of the authors, died on 22 March, 2004. This paper is dedicated
to her memor
Optical and Radio Properties of Extragalactic Sources Observed by the FIRST and SDSS Surveys
We discuss the optical and radio properties of 30,000 FIRST sources
positionally associated with an SDSS source in 1230 deg of sky. The
majority (83%) of the FIRST sources identified with an SDSS source brighter
than r=21 are optically resolved. We estimate an upper limit of 5% for the
fraction of quasars with broad-band optical colors indistinguishable from those
of stars. The distribution of quasars in the radio flux -- optical flux plane
supports the existence of the "quasar radio-dichotomy"; 8% of all quasars with
i<18.5 are radio-loud and this fraction seems independent of redshift and
optical luminosity. The radio-loud quasars have a redder median color by 0.08
mag, and a 3 times larger fraction of objects with red colors. FIRST galaxies
represent 5% of all SDSS galaxies with r<17.5, and 1% for r<20, and are
dominated by red galaxies. Magnitude and redshift limited samples show that
radio galaxies have a different optical luminosity distribution than non-radio
galaxies selected by the same criteria; when galaxies are further separated by
their colors, this result remains valid for both blue and red galaxies. The
distributions of radio-to-optical flux ratio are similar for blue and red
galaxies in redshift-limited samples; this similarity implies that the
difference in their luminosity functions, and resulting selection effects, are
the dominant cause for the preponderance of red radio galaxies in flux-limited
samples. We confirm that the AGN-to-starburst galaxy number ratio increases
with radio flux, and find that radio emission from AGNs is more concentrated
than radio emission from starburst galaxies (abridged).Comment: submitted to AJ, color gif figures, PS figures available from
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