2,824 research outputs found
Arginine deprivation, growth inhibition and tumour cell death: 2. Enzymatic degradation of arginine in normal and malignant cell cultures
Arginase added to culture medium reduced arginine to negligible levels within approximately 6 h, and enzyme activity persisted relatively undiminished for at least 3 days. Human and bovine arginase proved equally effective. The response of normal cells was to enter G1 (G0) arrest, from which most of the cells could be recovered weeks later. In contrast, malignant cell lines treated with unpegylated or pegylated enzyme resulted in cell death on a massive scale within 3 - 5 days, with a very low to negligible percentage of cells (8 days. Arginine decarboxylase caused malignant cell arrest at the same units per millilitre as arginase. Its breakdown product, agmatine, was relatively nontoxic in the presence of arginine, but exacerbated cell death above millimolar concentration in its absence. Although ornithine failed to rescue cells from deprivation, citrulline recovered cells in all cases, although less well in fast-growing tumour cell populations, whereas readdition of arginine failed to work unless a complete medium change was given (because of the persistence of the enzymes in the medium catabolising its destruction). The advantages and disadvantages of these two arginine-catabolising enzymes are discussed, and compared with arginine deiminase
Creating the Future of Microspace Technology
The Advanced Space Technology Program (ASTP) at the Defense Advanced Research Projects Agency (DARPA) has recently initiated a series of technology development efforts as part of its drive to enhance the cost effectiveness and responsiveness of defense space systems. These efforts focus on reducing spacecraft size, cost, weight, and power consumption, while simultaneously improving performance. The technology initiatives which are underway span a broad spectrum of efforts at the satellite system and subsystem levels, as well as some which focus on individual components and materials. The technology initiatives which DARPA is pursuing will enhance large, major satellites via technology insertions of cost effective, leading edge technologies in a timely fashion, and will facilitate a new class of small highly capable satellites. This paper will highlight some of the new efforts which have been initiated by ASTP in the past year
Relationship between the Linear Ringing Frequencies in 3He-A and 3He-B near the Polycritical Point
New measurements of parallel ringing in a quasi-ideal geometry for 3He-B near the temperature and pressure of the polycritical point suggest fB2/fA2∼5/2, where fB and fA are the linear parallel-ringing frequencies at a given temperature near Tc. This result approaches the prediction of theory using the Anderson-Brinkman-Morel and Balian-Werthamer states to describe 3He-A and 3He-B, respectively, and hence the results of Osheroff at melting pressure, but disagrees with earlier observations at pressures near the polycritical point
Relaxation of the Wall-Pinned Magnetization Ringing Mode in Superfluid 3He-B
Observations of the wall-pinned mode in 3He-B allow new magnetic relaxation phenomena to be studied. Excepting the quantitative value of the zero-time ringing frequency, comparison of experiment with theory is satisfactory, including a linear dependence of the square of the ringing period on time and a square-root singularity near Tc in the relaxation parameter
Nonlinear Parallel Ringing of Magnetization in Superfluid 3He
Experiments based on an analogy to the ac Josephson effect have shown in both 3He-A and 3He-B that a pairing theory of the superfluidity of 3He is essentially correct. Additional observations of parallel ringing are not in agreement with the simple pendulum models used to describe nonlinear dynamic magnetic effects
Upper Critical Field in a Spin-Charge Separated Superconductor
It is demonstrated that the spatial decay of the pair propagator in a
Luttinger liquid with spin charge separation contains a logarithmic correction
relative to the free fermi gas result in a finite interval between the spin and
charge thermal lengths. It is argued that similar effects can be expected in
higher dimensional systems with spin charge separation and that the temperature
dependence of the upper critical field curve is a probe of this
effect.Comment: 3 pages, postscript file (compressed and uuencoded
Thermodynamically consistent equilibrium properties of normal-liquid Helium-3
The high-precision data for the specific heat C_{V}(T,V) of normal-liquid
Helium-3 obtained by Greywall, taken together with the molar volume V(T_0,P) at
one temperature T_0, are shown to contain the complete thermodynamic
information about this phase in zero magnetic field. This enables us to
calculate the T and P dependence of all equilibrium properties of normal-liquid
Helium-3 in a thermodynamically consistent way for a wide range of parameters.
The results for the entropy S(T,P), specific heat at constant pressure
C_P(T,P), molar volume V(T,P), compressibility kappa(T,P), and thermal
expansion coefficient alpha(T,P) are collected in the form of figures and
tables. This provides the first complete set of thermodynamically consistent
values of the equilibrium quantities of normal-liquid Helium-3. We find, for
example, that alpha(T,P) has a surprisingly intricate pressure dependence at
low temperatures, and that the curves alpha(T,P) vs T do not cross at one
single temperature for all pressures, in contrast to the curves presented in
the comprehensive survey of helium by Wilks.
Corrected in cond-mat/9906222v3: The sign of the coefficient d_0 was
misprinted in Table I of cond-mat/9906222v1 and v2. It now correctly reads
d_0=-7.1613436. All results in the paper were obtained with the correct value
of d_0. (We would like to thank for E. Collin, H. Godfrin, and Y. Bunkov for
finding this misprint.)Comment: 19 pages, 19 figures, 9 tables; published version; note added in
proof; v3: misprint correcte
Concentration Dependence of the Effective Mass of He-3 Atoms in He-3/He-4 Mixtures
Recent measurements by Yorozu et al. (S. Yorozu, H. Fukuyama, and H.
Ishimoto, Phys. Rev. B 48, 9660 (1993)) as well as by Simons and Mueller (R.
Simons and R. M. Mueller, Czhechoslowak Journal of Physics Suppl. 46, 201
(1976)) have determined the effective mass of He-3 atoms in a He-3/He-4 mixture
with great accuracy. We here report theoretical calculations for the dependence
of that effective mass on the He-3 concentration. Using correlated basis
functions perturbation theory to infinite order to compute effective
interactions in the appropriate channels, we obtain good agreement between
theory and experiment.Comment: 4 pages, 1 figur
Progress in Monte Carlo calculations of Fermi systems: normal liquid 3He
The application of the diffusion Monte Carlo method to a strongly interacting
Fermi system as normal liquid He is explored. We show that the fixed-node
method together with the released-node technique and a systematic method to
analytically improve the nodal surface constitute an efficient strategy to
improve the calculation up to a desired accuracy. This methodology shows
unambiguously that backflow correlations, when properly optimized, are enough
to generate an equation of state of liquid He in excellent agreement with
experimental data from equilibrium up to freezing.Comment: 14 pages, 3 eps figure
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