Arginine deprivation, growth inhibition and tumour cell death: 2. Enzymatic degradation of arginine in normal and malignant cell cultures

Arginase added to culture medium reduced arginine to negligible levels within approximately 6 h, and enzyme activity persisted relatively undiminished for at least 3 days. Human and bovine arginase proved equally effective. The response of normal cells was to enter G1 (G0) arrest, from which most of the cells could be recovered weeks later. In contrast, malignant cell lines treated with unpegylated or pegylated enzyme resulted in cell death on a massive scale within 3 - 5 days, with a very low to negligible percentage of cells (8 days. Arginine decarboxylase caused malignant cell arrest at the same units per millilitre as arginase. Its breakdown product, agmatine, was relatively nontoxic in the presence of arginine, but exacerbated cell death above millimolar concentration in its absence. Although ornithine failed to rescue cells from deprivation, citrulline recovered cells in all cases, although less well in fast-growing tumour cell populations, whereas readdition of arginine failed to work unless a complete medium change was given (because of the persistence of the enzymes in the medium catabolising its destruction). The advantages and disadvantages of these two arginine-catabolising enzymes are discussed, and compared with arginine deiminase

Creating the Future of Microspace Technology

The Advanced Space Technology Program (ASTP) at the Defense Advanced Research Projects Agency (DARPA) has recently initiated a series of technology development efforts as part of its drive to enhance the cost effectiveness and responsiveness of defense space systems. These efforts focus on reducing spacecraft size, cost, weight, and power consumption, while simultaneously improving performance. The technology initiatives which are underway span a broad spectrum of efforts at the satellite system and subsystem levels, as well as some which focus on individual components and materials. The technology initiatives which DARPA is pursuing will enhance large, major satellites via technology insertions of cost effective, leading edge technologies in a timely fashion, and will facilitate a new class of small highly capable satellites. This paper will highlight some of the new efforts which have been initiated by ASTP in the past year

Relationship between the Linear Ringing Frequencies in 3He-A and 3He-B near the Polycritical Point

New measurements of parallel ringing in a quasi-ideal geometry for 3He-B near the temperature and pressure of the polycritical point suggest fB2/fA2∼5/2, where fB and fA are the linear parallel-ringing frequencies at a given temperature near Tc. This result approaches the prediction of theory using the Anderson-Brinkman-Morel and Balian-Werthamer states to describe 3He-A and 3He-B, respectively, and hence the results of Osheroff at melting pressure, but disagrees with earlier observations at pressures near the polycritical point

Relaxation of the Wall-Pinned Magnetization Ringing Mode in Superfluid 3He-B

Observations of the wall-pinned mode in 3He-B allow new magnetic relaxation phenomena to be studied. Excepting the quantitative value of the zero-time ringing frequency, comparison of experiment with theory is satisfactory, including a linear dependence of the square of the ringing period on time and a square-root singularity near Tc in the relaxation parameter

Nonlinear Parallel Ringing of Magnetization in Superfluid 3He

Experiments based on an analogy to the ac Josephson effect have shown in both 3He-A and 3He-B that a pairing theory of the superfluidity of 3He is essentially correct. Additional observations of parallel ringing are not in agreement with the simple pendulum models used to describe nonlinear dynamic magnetic effects

Upper Critical Field in a Spin-Charge Separated Superconductor

It is demonstrated that the spatial decay of the pair propagator in a Luttinger liquid with spin charge separation contains a logarithmic correction relative to the free fermi gas result in a finite interval between the spin and charge thermal lengths. It is argued that similar effects can be expected in higher dimensional systems with spin charge separation and that the temperature dependence of the upper critical field $H_{c2}$ curve is a probe of this effect.Comment: 3 pages, postscript file (compressed and uuencoded

Thermodynamically consistent equilibrium properties of normal-liquid Helium-3

The high-precision data for the specific heat C_{V}(T,V) of normal-liquid Helium-3 obtained by Greywall, taken together with the molar volume V(T_0,P) at one temperature T_0, are shown to contain the complete thermodynamic information about this phase in zero magnetic field. This enables us to calculate the T and P dependence of all equilibrium properties of normal-liquid Helium-3 in a thermodynamically consistent way for a wide range of parameters. The results for the entropy S(T,P), specific heat at constant pressure C_P(T,P), molar volume V(T,P), compressibility kappa(T,P), and thermal expansion coefficient alpha(T,P) are collected in the form of figures and tables. This provides the first complete set of thermodynamically consistent values of the equilibrium quantities of normal-liquid Helium-3. We find, for example, that alpha(T,P) has a surprisingly intricate pressure dependence at low temperatures, and that the curves alpha(T,P) vs T do not cross at one single temperature for all pressures, in contrast to the curves presented in the comprehensive survey of helium by Wilks. Corrected in cond-mat/9906222v3: The sign of the coefficient d_0 was misprinted in Table I of cond-mat/9906222v1 and v2. It now correctly reads d_0=-7.1613436. All results in the paper were obtained with the correct value of d_0. (We would like to thank for E. Collin, H. Godfrin, and Y. Bunkov for finding this misprint.)Comment: 19 pages, 19 figures, 9 tables; published version; note added in proof; v3: misprint correcte

Concentration Dependence of the Effective Mass of He-3 Atoms in He-3/He-4 Mixtures

Recent measurements by Yorozu et al. (S. Yorozu, H. Fukuyama, and H. Ishimoto, Phys. Rev. B 48, 9660 (1993)) as well as by Simons and Mueller (R. Simons and R. M. Mueller, Czhechoslowak Journal of Physics Suppl. 46, 201 (1976)) have determined the effective mass of He-3 atoms in a He-3/He-4 mixture with great accuracy. We here report theoretical calculations for the dependence of that effective mass on the He-3 concentration. Using correlated basis functions perturbation theory to infinite order to compute effective interactions in the appropriate channels, we obtain good agreement between theory and experiment.Comment: 4 pages, 1 figur

Progress in Monte Carlo calculations of Fermi systems: normal liquid 3He

The application of the diffusion Monte Carlo method to a strongly interacting Fermi system as normal liquid $^3$He is explored. We show that the fixed-node method together with the released-node technique and a systematic method to analytically improve the nodal surface constitute an efficient strategy to improve the calculation up to a desired accuracy. This methodology shows unambiguously that backflow correlations, when properly optimized, are enough to generate an equation of state of liquid $^3$He in excellent agreement with experimental data from equilibrium up to freezing.Comment: 14 pages, 3 eps figure