Expanding the Role of Social Class in Multicultural Counselor Education Curricula

Although professional counselors are called to develop Multicultural and Social Justice Counseling Competencies (MSJCC; Ratts et al., 2016), and social and cultural diversity are highly researched (e.g., Barrio Minton et al., 2014), social class as a multicultural construct has received less attention and is often reduced to being defined as socioeconomic status (SES). Therefore, this article provides a brief historical context of social class, explores the current state of social class research in counseling and counselor education, and suggests methods for increasing inclusion of social class in counselor educators’ multicultural pedagogy

Grief Content Inclusion in CACREP-Accredited Counselor Education Programs

This study investigated how counselor educators (n = 61) integrated grief content in accredited counselor education programs, characteristics of the course in which grief is taught, and professional background of faculty members. Most participants endorsed general content items (e.g., reactions to loss), grief theories (e.g., stages/phases of grief), and practice considerations (e.g., self-care). Additionally, results indicated grief-focused courses were mostly elective (n = 23; 85.19%), facilitated face-to-face (n = 18; 66.67%) and taught by counselor educators influenced by significant personal loss (n = 18, 78.26%). Results demonstrated a lack of professional affiliation with grief-related organizations, professional development through certifications, or continuing education. Discussion of results, implications for the counseling profession, and areas for future research and practice are explored

Exploring Possibilities of Predicting Positive Counselor Qualities in Counseling Students from Personality Domains

In this pilot study, the authors investigated the degree that Big Five personality domains may predict the positive counselor qualities among 160 students enrolled in sections of a combined undergraduate/graduate Counseling Skills course. Positive counselor qualities of focus in this study are empathy, mindfulness or self-awareness, and unconditional positive self-regard in students studying counseling skills. The results do not suggest a significant predictive role for the Big Five personality domains for the Counseling Skills students, except that the Big Five domain of neuroticism predicted enough variation in mindfulness and unconditional positive self-regard to be practically significant. Limitations and potential implications of these findings for counselor educators and for future research are discussed

Microbial activity in the marine deep biosphere: progress and prospects

The vast marine deep biosphere consists of microbial habitats within sediment, pore waters, upper basaltic crust and the fluids that circulate throughout it. A wide range of temperature, pressure, pH, and electron donor and acceptor conditions exists—all of which can combine to affect carbon and nutrient cycling and result in gradients on spatial scales ranging from millimeters to kilometers. Diverse and mostly uncharacterized microorganisms live in these habitats, and potentially play a role in mediating global scale biogeochemical processes. Quantifying the rates at which microbial activity in the subsurface occurs is a challenging endeavor, yet developing an understanding of these rates is essential to determine the impact of subsurface life on Earth\u27s global biogeochemical cycles, and for understanding how microorganisms in these “extreme” environments survive (or even thrive). Here, we synthesize recent advances and discoveries pertaining to microbial activity in the marine deep subsurface, and we highlight topics about which there is still little understanding and suggest potential paths forward to address them. This publication is the result of a workshop held in August 2012 by the NSF-funded Center for Dark Energy Biosphere Investigations (C-DEBI) “theme team” on microbial activity (www.darkenergybiosphere.org)

A modified sequence capture approach allowing standard and methylation analyses of the same enriched genomic DNA sample

Background: Bread wheat has a large complex genome that makes whole genome resequencing costly. Therefore, genome complexity reduction techniques such as sequence capture make re-sequencing cost effective. With a high-quality draft wheat genome now available it is possible to design capture probe sets and to use them to accurately genotype and anchor SNPs to the genome. Furthermore, in addition to genetic variation, epigenetic variation provides a source of natural variation contributing to changes in gene expression and phenotype that can be profiled at the base pair level using sequence capture coupled with bisulphite treatment. Here, we present a new 12 Mbp wheat capture probe set, that allows both the profiling of genotype and methylation from the same DNA sample. Furthermore, we present a method, based on Agilent SureSelect Methyl-Seq, that will use a single capture assay as a starting point to allow both DNA sequencing and methyl-seq. Results: Our method uses a single capture assay that is sequentially split and used for both DNA sequencing and methyl-seq. The resultant genotype and epi-type data is highly comparable in terms of coverage and SNP/methylation site identification to that generated from separate captures for DNA sequencing and methyl-seq. Furthermore, by defining SNP frequencies in a diverse landrace from the Watkins collection we highlight the importance of having genotype data to prevent false positive methylation calls. Finally, we present the design of a new 12 Mbp wheat capture and demonstrate its successful application to re-sequence wheat. Conclusions: We present a cost-effective method for performing both DNA sequencing and methyl-seq from a single capture reaction thus reducing reagent costs, sample preparation time and DNA requirements for these complementary analyses

Microbial activity in the marine deep biosphere: progress and prospects

The vast marine deep biosphere consists of microbial habitats within sediment, pore waters, upper basaltic crust and the fluids that circulate throughout it. A wide range of temperature, pressure, pH, and electron donor and acceptor conditions exists—all of which can combine to affect carbon and nutrient cycling and result in gradients on spatial scales ranging from millimeters to kilometers. Diverse and mostly uncharacterized microorganisms live in these habitats, and potentially play a role in mediating global scale biogeochemical processes. Quantifying the rates at which microbial activity in the subsurface occurs is a challenging endeavor, yet developing an understanding of these rates is essential to determine the impact of subsurface life on Earth's global biogeochemical cycles, and for understanding how microorganisms in these “extreme” environments survive (or even thrive). Here, we synthesize recent advances and discoveries pertaining to microbial activity in the marine deep subsurface, and we highlight topics about which there is still little understanding and suggest potential paths forward to address them.This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by Frontiers Research Foundation. The published article can be found at: http://www.frontiersin.org/Microbiology.Keywords: Subsurface microbiology, Biogeochemistry, C-DEBI, Deep biosphere, IODP, Sediment, Oceanic crustKeywords: Subsurface microbiology, Biogeochemistry, C-DEBI, Deep biosphere, IODP, Sediment, Oceanic crus

A haplotype map of allohexaploid wheat reveals distinct patterns of selection on homoeologous genomes

BACKGROUND: Bread wheat is an allopolyploid species with a large, highly repetitive genome. To investigate the impact of selection on variants distributed among homoeologous wheat genomes and to build a foundation for understanding genotype-phenotype relationships, we performed population-scale re-sequencing of a diverse panel of wheat lines. RESULTS: A sample of 62 diverse lines was re-sequenced using the whole exome capture and genotyping-by-sequencing approaches. We describe the allele frequency, functional significance, and chromosomal distribution of 1.57 million single nucleotide polymorphisms and 161,719 small indels. Our results suggest that duplicated homoeologous genes are under purifying selection. We find contrasting patterns of variation and inter-variant associations among wheat genomes; this, in addition to demographic factors, could be explained by differences in the effect of directional selection on duplicated homoeologs. Only a small fraction of the homoeologous regions harboring selected variants overlapped among the wheat genomes in any given wheat line. These selected regions are enriched for loci associated with agronomic traits detected in genome-wide association studies. CONCLUSIONS: Evidence suggests that directional selection in allopolyploids rarely acted on multiple parallel advantageous mutations across homoeologous regions, likely indicating that a fitness benefit could be obtained by a mutation at any one of the homoeologs. Additional advantageous variants in other homoelogs probably either contributed little benefit, or were unavailable in populations subjected to directional selection. We hypothesize that allopolyploidy may have increased the likelihood of beneficial allele recovery by broadening the set of possible selection targets

Developmental Networks and Interpersonal Support of Beginning Counselors

As beginning counselors complete graduate school and enter the workplace they often experience a turbulent transition (Moss et al., 2014). Interpersonal relationships play a vital role in counselors’ development during this period of change (Skovholt & Trotter-Mathison, 2011). This qualitative study explored beginning counselors’ perspectives regarding the role of interpersonal relationships in their development. Analysis of interviews with 12 beginning counselors (within two years post-graduation) resulted in five themes: Context of Personal-Professional Identity; Adjustment/Transition; Growth Orientation; Network Organization; and Connective Tissue. Implications suggest educators promote effective developmental relationships and encourage established counseling professionals to foster workplace environments that help beginning counselors navigate the transition

Farmers\u27 Concerns: A Qualitative Assessment to Plan Rural Medical Education

Abstract Context: Limited research suggests that translational approaches are needed to decrease the distance, physical and cultural, between farmers and health care. Purpose: This study seeks to identify special concerns of farmers in Alabama and explore the need for a medical education program tailored to prepare physicians to address those concerns. Methods: We conducted 2 focus groups with 20 farmers from diverse communities, backgrounds, and farming operations. The sessions were audio-recorded, transcribed, coded, and analyzed for determined patterns. Findings: The following categories were developed as areas of importance to farmers: the need for physicians to understand the culture of farming, occupational exposures in farming, and recommendations for improving the health of farmers. Conclusion: Findings suggest that to adequately serve farmers, medical students interested in entering practice in rural areas should have or develop a relevant and adequate understanding of farming practices