The Size Relationship of 12 Days Post-Exodus Larvae with 56 Day Post-Exodus Fingerlings Regarding Growth and Survival in Bluegill (Lempomis macrochirus)

Low survival and quality of early life-stage bluegill is an obstacle to viable production where size may influence weaning efficiency. Herein, we investigate through two trials the effects of fry size 12-d post-exodus on total length, weight, production and survival through 56-d post-exodus. Full sibling broods (trial 1=10, trial 2=6) were reared with feedings of brine shrimp Artemia sp. nauplii (BS) through 12-d post-exodus (PE). Broods of trial 1 were visual sorted 12 d PE into small and large size groups. Fry (n = 25) from each size group were sampled for measured total length (TL) and weight. Starting 14 d PE, a 7-d co-feeding period with a commercial dry feed (#0 crumb) that was continued through 27 d PE with subsequent feedings using a larger version (#1 crumb) of the same diet continuing through 56 d PE (trial end). Broods of trial 2 were split 4 d post-conception into two gorups of pro-larvae (n = 400). Slow growing groups were fed hourly 0800-1700 while fast growing were fed additionally at 0400 and 2200 through 12 d PE. Surviving fish from each size gorup were collected, with random samples (n = 25 fish) measured for TL and weight. Fry (n = 100 where possible) were placed into 151-L aqauriums with slow and fast growing feed regimens maintained through 13-d PE. Culture regimen thereafter was same as trial 1. Fish of both trials were harvested, measured for TL, weight, and survival. The Wilcoxon Signed Rank Test was run one-tailed using SAS. Results of trial 1 indicate size at 12 d was a predictor of TL, weight and survival through 56 d PE (p ≤ 0.156). Size variation at 12 d as a function of genetics is a predictor of growth and survival through 56 d while at least some size variation resulting from early growth rate differences can be overcome by compensatory growth.https://bluetigercommons.lincolnu.edu/lucer-pubs/1007/thumbnail.jp

Daily Increment Formation in Otoliths of the Redspotted Sunfish

To generate reliable age information for fishes, we must assess the robustness of techniques to environmental variation. We quantified daily ring deposition within the sagittal otoliths of known-age larval and juvenile redspotted sunfish Lepomis miniatus reared in ponds. We also determined how daily light : dark cycles mimicking natural conditions, reduced daylight, or constant daylight affected ring deposition in fish housed in aquaria. Additionally, we quantified how constant temperature (26°C), low daily variability in temperature (26 ± 1°C), and high daily variability (26 ± 2°C) affected production of daily rings. In pond-reared fish, the first increment was produced by the evening of hatch, and increment deposition occurred with 24-h periodicity through 119 d. Independent age estimates from two readers closely agreed. Photocycle and temperature variation did not affect periodicity of increment formation or the precision and accuracy of age estimates. Age and growth data can be reliably determined in this species to assess recruitment dynamics and develop management and conservation strategies

Amino Acid Profiles in the Tissues of Juvenile Bluegill (Lepomis Macrochirus) from the Wild and Commercially-fed Fish

Amino acid profiles of fish tissues and fish eggs have been extensively investigated in some fish species. Amino acid profiles are helpful in quantifying the indispensable amino acid requirements for these fish. Bluegill is a major forage fish for the largemouth bass (Micropterus samoides) and an important recreational fish throughout the US. The demand for bleugill as a food fish has increased recently. However, the information on the amino acid profiles of its eggs and tissues is lacking. The objectve of this study was to determine the amino acid profiles of the muscles and whole body of the 1-2 g wild and the commercially fed bluegill. Wild fish were obtained from the ponds that relied on the natural food source. Commercially fed fish were grown indoors. Skinless muscles were obtained by dissected both sides of the 1-2 g bluegill. The results showed the lysine was the most abundant indispensable amino acids in muscles and hwole body of both the wild and the farm-raised fish. The proportion of an individual indispensable amino acid to the sum of all detectable amino acids amount was similar for whole body and muscles in wild or raised fish. For whole body or muscle tissue, wild fish contained significantly higher crude protein but lower crude lipid levels than farm-raised bluegill (p\u3c0.05). This study suggested that nutritional history affected tissue proximate compositions.https://bluetigercommons.lincolnu.edu/lucer-pubs/1003/thumbnail.jp

Communication and signal exchange in the Rhizobium bradyrhizobium legume system

A new comprehensive communication concept in the Rhizobium/Bradyrhizobium legume symbiosis was developed. It includes a root zone specific flavonoid exudation, the differential activity of phenylpropane/acetate pathway derivatives on chemotaxis, nod-gene inducing activity and phytoalexin resistance induction on the microsymbiont side (Bradyrhizobium). Nod factor production from the microsymbiont affects the host plant in root hair curling and meristem induction. Phytoalexin production in the host plant is also an early response, however repressed to a low level after a few hours. Another strategy of the microsymbiont to overcome phytoalexin effects is degradation of phytoalexins in Rhizobium leguminosarum bv. vicieae. Competitiveness within the same infection group of the microsymbiont was studied with gus-gene fusion, using the blue coloured nodules to easily discriminate marked strains from unmarked competitors. New exopolysaccharide (EPS) mutants of Bradyrhizobium japonicum were reconstructed homologous with a DNA region to exoB gene of Rhizobium meliloti. Their clearly reduced competitiveness of nodulation, demonstrates that exopolysaccharides of Bradyrhizohium japonicum also have an important function during the early stages of this symbiotic interaction

Comparison of Pond Production of Phase-III Sunshine Bass Fed 32-, 36-, and 40%-Crude-Protein Diets with Fixed Energy : Protein Ratios

We stocked phase-III sunshine bass (white bass Morone chrysops ♀ × striped bass M. saxatilis ♂) at a rate of 6,188 fingerlings/ha into twelve 0.04-ha earthen ponds supplied with continuous aeration. Three dietary treatments were randomly assigned to quadruplicate ponds. Sunshine bass were fed to apparent satiation once daily after average initial weight (mean ± SE = 214 ± 5 g) and total length (245 ± 1.6 mm) were determined. Diets were formulated to conserve the estimated digestible energy:crude protein (CP) ratio (9.3 kcal/g protein) and represented the following CP and energy values fed to fish: 32% CP (3,000 kcal/kg), 36% CP (3,360 kcal/kg), and 40% CP (3,760 kcal/kg). Harvest data suggest that nutrient density is a variable that can be manipulated to optimize production and reduce production costs. Production rates (mean ± SE) were 2,851 ± 600 kg/ha for the 32%-CP diet, 2,895 ± 341 kg/ha for the 36%-CP diet, and 2,953 ± 142 kg/ha for the 40%-CP diet; production rates were not significantly different among dietary treatments. Survival was excellent and did not appear to be related to dietary treatment. Dressed (gilled and gutted) fish averaged 80% of whole-fish weight, and the dressed percentage did not vary as a function of nutrient density. Feed conversion ratios of 3.0 ± 0.4, 2.8 ± 0.2, and 2.6 ± 0.1 were obtained for the fish fed 32-, 36-, and 40%-CP diets, respectively. Protein conversion ratios (mean = 1.0) were not significantly influenced by dietary treatment. Feed cost increased with increasing dietary CP level; costs were US$0.447 per kilogram for the 32$0.493 per kilogram for the 36%-CP diet, and $0.541 per kilogram for the 40$1.34, $1.38, and$1.41 per kilogram of gain for the 32-, 36-, and 40%-CP diets, respectively. A savings of $0.16 per kilogram produced, or approximately$450 per hectare, was realized as a result of feeding either of the two lower-CP, lower-energy diets. Accordingly, we suggest that phase-III sunshine bass can be more economically produced by feeding diets as low as 32% CP with a minimum energy:protein ratio of 9.3 kcal/g CP

Stars made in outflows may populate the stellar halo of the Milky Way

We study stellar-halo formation using six Milky-Way-mass galaxies in FIRE-2 cosmological zoom simulations. We find that 5−40 per cent of the outer (50–300 kpc) stellar halo in each system consists of in-situ stars that were born in outflows from the main galaxy. Outflow stars originate from gas accelerated by superbubble winds, which can be compressed, cool, and form co-moving stars. The majority of these stars remain bound to the halo and fall back with orbital properties similar to the rest of the stellar halo at z = 0. In the outer halo, outflow stars are more spatially homogeneous, metal-rich, and alpha-element-enhanced than the accreted stellar halo. At the solar location, up to ∼10 per cent of our kinematically identified halo stars were born in outflows; the fraction rises to as high as ∼40 per cent for the most metal-rich local halo stars ([Fe/H] >−0.5). Such stars can be retrograde and create features similar to the recently discovered Milky Way ‘Splash’ in phase space. We conclude that the Milky Way stellar halo could contain local counterparts to stars that are observed to form in molecular outflows in distant galaxies. Searches for such a population may provide a new, near-field approach to constraining feedback and outflow physics. A stellar halo contribution from outflows is a phase-reversal of the classic halo formation scenario of Eggen, Lynden-Bell & Sandange, who suggested that halo stars formed in rapidly infalling gas clouds. Stellar outflows may be observable in direct imaging of external galaxies and could provide a source for metal-rich, extreme-velocity stars in the Milky Way

Astro2020 APC White Paper: Theoretical Astrophysics 2020-2030

The past two decades have seen a tremendous investment in observational facilities that promise to reveal new and unprecedented discoveries about the universe. In comparison, the investment in theoretical work is completely dwarfed, even though theory plays a crucial role in the interpretation of these observations, predicting new types of phenomena, and informing observing strategies. In this white paper, we argue that in order to reach the promised critical breakthroughs in astrophysics over the next decade and well beyond, the national agencies must take a serious approach to investment in theoretical astrophysics research. We discuss the role of theory in shaping our understanding of the universe, and then we provide a multi-level strategy, from the grassroots to the national, to address the current underinvestment in theory relative to observational work

The origin of the diverse morphologies and kinematics of Milky Way-mass galaxies in the FIRE-2 simulations

We use hydrodynamic cosmological zoom-in simulations from the Feedback in Realistic Environments project to explore the morphologies and kinematics of 15 Milky Way (MW)-mass galaxies. Our sample ranges from compact, bulge-dominated systems with 90 per cent of their stellar mass within 2.5 kpc to well-ordered discs that reach ≳15 kpc. The gas in our galaxies always forms a thin, rotation-supported disc at z = 0, with sizes primarily determined by the gas mass. For stars, we quantify kinematics and morphology both via the fraction of stars on disc-like orbits and with the radial extent of the stellar disc. In this mass range, stellar morphology and kinematics are poorly correlated with the properties of the halo available from dark matter-only simulations (halo merger history, spin, or formation time). They more strongly correlate with the gaseous histories of the galaxies: those that maintain a high gas mass in the disc after z ∼ 1 develop well-ordered stellar discs. The best predictor of morphology we identify is the spin of the gas in the halo at the time the galaxy formed 1/2 of its stars (i.e. the gas that builds the galaxy). High-z mergers, before a hot halo emerges, produce some of the most massive bulges in the sample (from compact discs in gas-rich mergers), while later-forming bulges typically originate from internal processes, as satellites are stripped of gas before the galaxies merge. Moreover, most stars in z = 0 MW-mass galaxies (even z = 0 bulge stars) form in a disc: ≳60--90 per cent of stars begin their lives rotationally supported