Isolation and characterization of Nylanderia fulva virus 1, a positive-sense, single-stranded RNA virus infecting the tawny crazy ant, Nylanderia fulva.

We report the discovery of Nylanderia fulva virus 1 (NfV-1), the first virus identified and characterized from the ant, Nylanderia fulva. The NfV-1 genome (GenBank accession KX024775) is 10,881 nucleotides in length, encoding one large open reading frame (ORF). Helicase, protease, RNA-dependent RNA polymerase, and jelly-roll capsid protein domains were recognized within the polyprotein. Phylogenetic analysis placed NfV-1 in an unclassified clade of viruses. Electron microscopic examination of negatively stained samples revealed particles with icosahedral symmetry with a diameter of 28.7±1.1nm. The virus was detected by RT-PCR in larval, pupal, worker and queen developmental stages. However, the replicative strand of NfV-1 was only detected in larvae. Vertical transmission did not appear to occur, but horizontal transmission was facile. The inter-colonial field prevalence of NfV-1 was 52±35% with some local infections reaching 100%. NfV-1 was not detected in limited samples of other Nylanderia species or closely related ant species.JSL was supported by the National Science Foundation under Grant DEB-0743542. AEF is supported by the Wellcome Trust (Grant no. [106207]) and the European Research Council (ERC) under the European Union׳s Horizon 2020 research and innovation programme (Grant agreement no. [646891]).This is the final version of the article. It first appeared from Elsevier at https://doi.org/10.1016/j.virol.2016.06.014

The eventization of leisure and the strange death of alternative Leeds

The communicative potential of city spaces as leisure spaces is a central assumption of political activism and the creation of alternative, counter-cultural and subcultural scenes. However, such potential for city spaces is limited by the gentrification, privatization and eventization of city centres in the wake of wider societal and cultural struggles over leisure, work and identity formation. In this paper, we present research on alternative scenes in the city of Leeds to argue that the eventization of the city centre has led to a marginalization and of alternative scenes on the fringes of the city. Such marginalization has not caused the death of alternative Leeds or political activism associated with those scenes—but it has changed the leisure spaces (physical, political and social) in which alternative scenes contest the mainstream

Substructure revealed by RR Lyraes in SDSS Stripe 82

We present an analysis of the substructure revealed by 407 RR Lyraes in Sloan Digital Sky Survey (SDSS) Stripe 82. Period estimates are determined to high accuracy using a string-length method. A subset of 178 RR Lyraes with spectrally derived metallicities are employed to derive metallicity-period-amplitude relations, which are then used to find metallicities and distances for the entire sample. The RR Lyraes lie between 5 and 115 kpc from the Galactic center. They are divided into subsets of 316 RRab types and 91 RRc types based on their period, colour and metallicity. The density distribution is not smooth, but dominated by clumps and substructure. Samples of 55 and 237 RR Lyraes associated with the Sagittarius Stream and the Hercules-Aquila Cloud respectively are identified. Hence, ~ 70 % of the RR Lyraes in Stripe 82 belong to known substructure. There is a sharp break in the density distribution at Galactocentric radii of 40 kpc, reflecting the fact that the dominant substructure in Stripe 82 - the Hercules-Aquila Cloud and the Sagittarius Stream - lies within 40 kpc. In fact, almost 60 % of all the RR Lyraes in Stripe 82 are associated with the Hercules-Aquila Cloud alone, which emphasises its pre-eminence. Additionally, evidence of a new and distant substructure - the Pisces Overdensity - is found, consisting of 28 faint RR Lyraes centered on Galactic coordinates (80 deg, -55 deg) and with distances of ~ 80 kpc. The total stellar mass in the Pisces Overdensity is ~10000 solar masses and its metallicity is [Fe/H] ~ -1.5.Comment: 15 pages, submitted to MNRA

Why do we need (another) special issue on gender and VET?

The Journal of Vocational Education and Training last had a special issue on gender and vocational education and training (VET) in 2006. In the intervening 8 years, the journal has published 264 research papers, of which just 10 - that is, less than 4% - addressed the issue of gender in any substantive way. This is something of a disappointment, as editors always hope that a special issue will provoke increased discussion and debate in the journal about its theme as well as providing a focused resource. Is the problem that, compared to the effects of global labour migration flows, constantly rising youth unemployment figures or emerging skills gaps in an aging society, gender issues have lost their relevance as just one category of social inequalities among many others? This can hardly be the case, since it is obviously a most thorough-going and persistent one. Despite over 30 years of gender studies, and numerous policy initiatives to address gender inequalities, gender segregation persists in VET as well as in the labour market. Women still earn less, hold the majority of part-time contracts, tend to be stereotyped into caring and personal service work, and are largely under-represented in leadership positions. Is the problem then that there is nothing new to be said about gender and VET? We do not agree that that is the case either: gender injustice remains a central issue in VET and the labour market; and as the social, political and economic landscape changes rapidly in today's world, it is inevitable that gender inequalities are produced and reproduced in new and different ways. So we felt the need to call once again for a collection of papers dedicated specifically to this topic, and one that would reflect this evolution

Genetic Structure, Nestmate Recognition and Behaviour of Two Cryptic Species of the Invasive Big-Headed Ant Pheidole megacephala

info:eu-repo/semantics/publishe

All You Can Eat: High Performance Capacity and Plasticity in the Common Big-Eared Bat, Micronycteris microtis (Chiroptera: Phyllostomidae)

Ecological specialization and resource partitioning are expected to be particularly high in the species-rich communities of tropical vertebrates, yet many species have broader ecological niches than expected. In Neotropical ecosystems, Neotropical leaf-nosed bats (Phyllostomidae) are one of the most ecologically and functionally diverse vertebrate clades. Resource partitioning in phyllostomids might be achieved through differences in the ability to find and process food. We selected Micronycteris microtis, a very small (5–7 g) animalivorous phyllostomid, to explore whether broad resource use is associated with specific morphological, behavioral and performance traits within the phyllostomid radiation. We documented processing of natural prey and measured bite force in free-ranging M. microtis and other sympatric phyllostomids. We found that M. microtis had a remarkably broad diet for prey size and hardness. For the first time, we also report the consumption of vertebrates (lizards), which makes M. microtis the smallest carnivorous bat reported to date. Compared to other phyllostomids, M. microtis had the highest bite force for its size and cranial shape and high performance plasticity. Bite force and cranial shape appear to have evolved rapidly in the M. microtis lineage. High performance capacity and high efficiency in finding motionless prey might be key traits that allow M. microtis, and perhaps other species, to successfully co-exist with other gleaning bats

Metatranscriptomics and Pyrosequencing Facilitate Discovery of Potential Viral Natural Enemies of the Invasive Caribbean Crazy Ant, Nylanderia pubens

BACKGROUND: Nylanderia pubens (Forel) is an invasive ant species that in recent years has developed into a serious nuisance problem in the Caribbean and United States. A rapidly expanding range, explosive localized population growth, and control difficulties have elevated this ant to pest status. Professional entomologists and the pest control industry in the United States are urgently trying to understand its biology and develop effective control methods. Currently, no known biological-based control agents are available for use in controlling N. pubens. METHODOLOGY AND PRINCIPAL FINDINGS: Metagenomics and pyrosequencing techniques were employed to examine the transcriptome of field-collected N. pubens colonies in an effort to identify virus infections with potential to serve as control agents against this pest ant. Pyrosequencing (454-platform) of a non-normalized N. pubens expression library generated 1,306,177 raw sequence reads comprising 450 Mbp. Assembly resulted in generation of 59,017 non-redundant sequences, including 27,348 contigs and 31,669 singlets. BLAST analysis of these non-redundant sequences identified 51 of potential viral origin. Additional analyses winnowed this list of potential viruses to three that appear to replicate in N. pubens. CONCLUSIONS: Pyrosequencing the transcriptome of field-collected samples of N. pubens has identified at least three sequences that are likely of viral origin and, in which, N. pubens serves as host. In addition, the N. pubens transcriptome provides a genetic resource for the scientific community which is especially important at this early stage of developing a knowledgebase for this new pest

Paleodistributions and Comparative Molecular Phylogeography of Leafcutter Ants (Atta spp.) Provide New Insight into the Origins of Amazonian Diversity

The evolutionary basis for high species diversity in tropical regions of the world remains unresolved. Much research has focused on the biogeography of speciation in the Amazon Basin, which harbors the greatest diversity of terrestrial life. The leading hypotheses on allopatric diversification of Amazonian taxa are the Pleistocene refugia, marine incursion, and riverine barrier hypotheses. Recent advances in the fields of phylogeography and species-distribution modeling permit a modern re-evaluation of these hypotheses. Our approach combines comparative, molecular phylogeographic analyses using mitochondrial DNA sequence data with paleodistribution modeling of species ranges at the last glacial maximum (LGM) to test these hypotheses for three co-distributed species of leafcutter ants (Atta spp.). The cumulative results of all tests reject every prediction of the riverine barrier hypothesis, but are unable to reject several predictions of the Pleistocene refugia and marine incursion hypotheses. Coalescent dating analyses suggest that population structure formed recently (Pleistocene-Pliocene), but are unable to reject the possibility that Miocene events may be responsible for structuring populations in two of the three species examined. The available data therefore suggest that either marine incursions in the Miocene or climate changes during the Pleistocene—or both—have shaped the population structure of the three species examined. Our results also reconceptualize the traditional Pleistocene refugia hypothesis, and offer a novel framework for future research into the area

Intersexuality and Trans-Identities within the Diversity Management Discourse

Within both the scientific discourse on workforce diversity, and diversity management practice, intersexuality and transgender issues have hitherto remained marginalized topics. This chapter gives an overview of the discourses on both phenomena, and proposes starting points for more inclusive organizational diversity management initiatives. It is shown that both topics represent different aspects of the category of "gender". The common practice of conceptually lumping together intersexuality, transgenderism, and sexual orientation can be seen as one important reason that intersexuality and transgenderism are rarely considered in organizational diversity management programs in terms of concrete action. Against this background, a modified, and more integrated approach to structuring the workforce alongside the different dimensions of diversity is proposed. It is shown that the categories of "biological sex and gender", "gender identity", and "sexual orientation" cannot be regarded as being separate from each other. They represent, rather, an interrelated organizational field of action that should be considered as being one interrelated topic for organizational diversity practices. This chapter derives this claim theoretically and discusses the consequences for organizational diversity management practices. For most organizations, this would mean a fundamental rethinking of their goals, in terms of workforce diversity, and the shaping of their diversity management programs