Characteristics and risk factors for symptomatic Giardia lamblia infections in Germany

Background: In developed countries, giardiasis is considered a travel related disease. However, routine surveillance data from Germany indicate that >50% of infections were acquired indigenously. We studied the epidemiological characteristics of symptomatic Giardia infections acquired in Germany and abroad, and verified the proportion of cases acquired in Germany in order to investigate risk factors for sporadic autochthonous Giardia infections. Methods: We identified Giardia cases notified by 41 local health authorities between February 2007 and January 2008 and interviewed them on their clinical symptoms, underlying morbidities, travel abroad and potential risk factors for the disease. We conducted a case-control-study including laboratory-confirmed (microscopy or antigen-test) autochthonous Giardia cases with clinical manifestations (diarrhoea, cramps, bloating) and randomly selected controls from the local population registry matched by county of residence and age-group (0-5, 6-19, ≥20 years). Secondary cases, controls with diarrhoea and persons who had travelled outside Germany in the three weeks prior to disease onset (exposure period) were excluded. We calculated adjusted odds ratios (aOR) with 95% confidence intervals (CI) using conditional logistic regression. Results: Of 273 interviewed cases, 131 (48%) had not travelled abroad during the defined exposure period. Of these 131, 85 (65%) were male, 68 (54%) were living in communities with >100,000 inhabitants and 107 (83%) were aged 20 years or older. We included 120 cases and 240 controls in the case-control study. Cases were more likely to be male (aOR 2.5 CI 1.4-4.4), immunocompromised (aOR 15.3 CI 1.8-127) and daily consumers of green salad (aOR 2.9 CI 1.2-7.2). Contact with animals (pets/farm animals) and exposure to surface water (swimming/water sports) were not associated with symptomatic disease. Conclusions: A substantial proportion of Giardia lamblia cases in Germany are indigenously acquired. Symptomatic cases are significantly more likely to be immunocompromised than control persons from the general population. Physicians should consider Giardia infections among patients with no recent history of travel abroad, particularly if they have immune deficiencies. Green salads may be an important vehicle of infection. Information campaigns highlighting this food-borne risk should emphasise the risk to persons with immune deficiencies

Aktueller Nipahvirus- Ausbruch im Distrikt Kozhikode im Norden des Bundestaates Kerala, Südindien

Peer Reviewe

The zoonotic potential of Clostridium difficile from small companion animals and their owners

Background: Clostridium difficile infections (CDI) in humans range from asymptomatic carriage to life-threatening intestinal disease. Findings on C. difficile in various animal species and an overlap in ribotypes (RTs) suggest potential zoonotic transmission. However, the impact of animals for human CDI remains unclear. Methods: In a large-scale survey we collected 1,447 fecal samples to determine the occurrence of C. difficile in small companion animals (dogs and cats) and their owners and to assess potential epidemiological links within the community. The Germany-wide survey was conducted from July 2012-August 2013. PCR ribotyping, Multilocus VNTR Analysis (MLVA) and PCR detection of toxin genes were used to characterize isolated C. difficile strains. A database was defined and logistic regression used to identify putative factors associated with fecal shedding of C. difficile. Results: In total, 1,418 samples met the inclusion criteria. The isolation rates for small companion animals and their owners within the community were similarly low with 3.0% (25/840) and 2.9% (17/578), respectively. PCR ribotyping revealed eight and twelve different RTs in animals and humans, respectively, whereas three RTs were isolated in both, humans and animals. RT 014/0, a well-known human hospital-associated lineage, was predominantly detected in animal samples. Moreover, the potentially highly pathogenic RTs 027 and 078 were isolated from dogs. Even though, C. difficile did not occur simultaneously in animals and humans sharing the same household. The results of the epidemiological analysis of factors associated with fecal shedding of C. difficile support the hypothesis of a zoonotic potential. Conclusions: Molecular characterization and epidemiological analysis revealed that the zoonotic risk for C. difficile associated with dogs and cats within the community is low but cannot be excluded

Survey zur ärztlichen Verschreibung von Antibiotika: Ergebnisse zur akuten Zystitis in der ambulanten Versorgung

Hintergrund: Vor dem Hintergrund steigender Resistenzraten werden Einflussfaktoren auf die Verschreibung spezifischer Antibiotika bei unkomplizierter Zystitis in der ambulanten Versorgung untersucht. Methoden: Nationale Querschnitterhebung im Jahr 2008 bei niedergelassenen Ärzten (Innere Medizin und Allgemeinmedizin, Chirurgie, Frauenheilkunde und Geburtshilfe, Kinder- und Jugendmedizin, Hals-, Nasen- und Ohrenheilkunde, Dermatologie sowie Urologie), Stichprobenziehung auf Basis der Facharztregister. Ergebnisse: 60 % der befragten Ärzte (n = 1 810) treffen täglich Entscheidungen über die Einleitung einer antibiotischen Therapie, wobei „unkomplizierter Harnwegsinfekt“ die häufigste Diagnose ist (n = 715). Die Mehrzahl der Befragten gibt an, bei dieser Diagnose Cotrimoxazol (61 %) zu verschreiben, gefolgt von Fluorchinolonen (21 %). Die folgenden Faktoren weisen eine statistisch auffällige Assoziation mit der Präferenz für Fluorchinolone auf: Fachrichtung Gynäkologie (Odds Ratio [OR] 0,47; Konfidenzintervall [KI]: 0,27–0,80), Praxisstandort in den neuen Bundesländern (OR 2,01; KI: 1,16–3,46), ein Verordnungsverhalten, das eine Umstellung von einer empirischen Therapie auf eine gezielte Therapie beinhaltet (OR 1,72; KI: 1,02–2,90), sowie die Absicht, dem Patienten Unannehmlichkeiten ersparen zu wollen (OR 2,14; KI: 1,25–3,68). Diskussion: Obwohl Fluorchinolone aufgrund der Resistenzentwicklung nicht mehr als Mittel der ersten Wahl empfohlen werden, werden sie noch häufig verordnet. ARS – Antibiotika-Resistenz-Surveillance in Deutschland – veröffentlicht aktuelle regionale und Patientengruppen-spezifische Resistenzraten, um gute klinische Praxis zu fördern und die Qualität der Verordnungen zu verbessern.Background: In view of the currently increasing rates of antibiotic resistance, we studied the factors that affect the prescribing of specific antibiotics for uncomplicated cystitis in outpatient care. Methods: A nationwide cross-sectional survey of physicians in private practice in various specialties (internal medicine, general medicine, surgery, obstetrics/gynecology, child and adolescent medicine, otorhinolaryngology, dermatology, urology) was carried out in 2008. The sample was derived from the German state directories of medical specialists. Results: 1810 (60%) of the physicians surveyed reported that they made decisions about antibiotic treatment every day, with uncomplicated urinary tract infection as the most common diagnosis (715 physicians). The antibiotics that they prescribed most commonly for it were cotrimoxazole (61%) and fluoroquinolones (21%). The following factors were significantly associated with a preference for fluoroquinolones: being a gynecologist (odds ratio [OR] 0.47, 95% confidence interval [CI] 0.27–0.80), location of practice in the former East Germany (OR 2.01, CI 1.16–3.46), a treatment strategy incorporating a switch from empirical to targeted treatment (OR 1.72, CI 1.02–2.90), and the stated intention of avoiding inconvenience to the patient (OR 2.14, CI 1.25–3.68). Discussion: Fluoroquinolones are no longer recommended as the drug of first choice for uncomplicated urinary tract infections because of the development of resistance, but are still commonly prescribed for it. ARS (Antibiotic Resistance Surveillance in Germany) publishes current regional and patient-group-specific resistance rates to promote good clinical practice and improve prescribing behavior

Molecular phylogeny of a novel human adenovirus type 8 strain causing a prolonged, multi-state keratoconjunctivitis epidemic in Germany

The German infectious disease surveillance system revealed an increase of epidemic keratoconjunctivitis (EKC) from an average of 320 cases/year (2001 to 2010) up to 2146 and 1986 cases in 2012 and 2013, respectively. From November 2011 until December 2013 (epidemic period) 85% of typed isolates were human adenovirus type 8 (HAdV-D8), whereas only low level circulation (19%) of HAdV-D8 was observed outside the epidemic period. In order to investigate whether a novel monophyletic HAdV-D8 strain prevailed during the epidemic period, complete genomic sequences of 23 HAdV-D8 isolates were generated by deep sequencing and analyzed phylogenetically. For comparison, eight HAdV-D8 isolates from outside the epidemic period were sequenced. HAdV-D8 isolates of the epidemic period had a very high sequence identity of at least 99.9% and formed a monophyletic cluster with two subclusters. A single outlier was closely related to HAdV-D8 strains isolated prior to the epidemic period. Circulation of the epidemic strain was detected as early as 2010 but not after the epidemic period in 2014. In conclusion, molecular phylogeny of complete genomic sequences proved a monophyletic HAdV-D8 epidemic. However, co-circulation of other HAdV types as well as better reporting may have contributed to the huge increase of reported cases

Characteristics and risk factors for symptomatic <it>Giardia lamblia </it>infections in Germany

Abstract Background In developed countries, giardiasis is considered a travel related disease. However, routine surveillance data from Germany indicate that >50% of infections were acquired indigenously. We studied the epidemiological characteristics of symptomatic Giardia infections acquired in Germany and abroad, and verified the proportion of cases acquired in Germany in order to investigate risk factors for sporadic autochthonous Giardia infections. Methods We identified Giardia cases notified by 41 local health authorities between February 2007 and January 2008 and interviewed them on their clinical symptoms, underlying morbidities, travel abroad and potential risk factors for the disease. We conducted a case-control-study including laboratory-confirmed (microscopy or antigen-test) autochthonous Giardia cases with clinical manifestations (diarrhoea, cramps, bloating) and randomly selected controls from the local population registry matched by county of residence and age-group (0-5, 6-19, ≥20 years). Secondary cases, controls with diarrhoea and persons who had travelled outside Germany in the three weeks prior to disease onset (exposure period) were excluded. We calculated adjusted odds ratios (aOR) with 95% confidence intervals (CI) using conditional logistic regression. Results Of 273 interviewed cases, 131 (48%) had not travelled abroad during the defined exposure period. Of these 131, 85 (65%) were male, 68 (54%) were living in communities with >100,000 inhabitants and 107 (83%) were aged 20 years or older. We included 120 cases and 240 controls in the case-control study. Cases were more likely to be male (aOR 2.5 CI 1.4-4.4), immunocompromised (aOR 15.3 CI 1.8-127) and daily consumers of green salad (aOR 2.9 CI 1.2-7.2). Contact with animals (pets/farm animals) and exposure to surface water (swimming/water sports) were not associated with symptomatic disease. Conclusions A substantial proportion of Giardia lamblia cases in Germany are indigenously acquired. Symptomatic cases are significantly more likely to be immunocompromised than control persons from the general population. Physicians should consider Giardia infections among patients with no recent history of travel abroad, particularly if they have immune deficiencies. Green salads may be an important vehicle of infection. Information campaigns highlighting this food-borne risk should emphasise the risk to persons with immune deficiencies.</p

The global measles crisis—a diversity of causes from armed conflicts to vaccination skepticism

Hintergrund und Ziel: Obwohl seit vielen Jahren ein sicherer und effektiver Impfstoff verfügbar ist, nehmen Fälle von Masern seit 2018 weltweit wieder zu. Ziel dieses Berichts ist die Identifizierung möglicher Gründe für diese Entwicklung. Methode: Es erfolgte eine selektive Literaturrecherche sowie die Auswertung aktueller Berichte und Daten der Weltgesundheitsorganisation (WHO), des Kinderhilfswerkes der Vereinten Nationen (UNICEF) und der Weltbank. Ergebnisse: Madagaskar, die Ukraine und Israel wiesen laut WHO im Zeitraum vom 01.07.2018 bis 30.06.2019 die weltweit höchsten Inzidenzen für Masern auf. Masernausbrüche sind ein Zeichen unzureichender Impfquoten, die durch vielfältige strukturelle und psychologische Barrieren verursacht werden. Strukturelle Barrieren für Masernimpfungen, wie mangelnde Routineimpfprogramme, bestehen vor allem in fragilen Ländern u. a. durch bewaffnete Konflikte. Sie wurden jedoch auch in Subpopulationen einkommensstärkerer Länder als Hauptursachen für geringe Masernimpfquoten u. a. durch fehlende Ressourcen für Impfdienste identifiziert. Psychologische Barrieren und nachfolgende Impfskepsis waren hauptsächlich in entwickelten Ländern mit gut funktionierenden Gesundheitssystemen und hohem Lebensstandard verbreitet. Diskussion: Die Gründe für die globale Masernkrise sind vielfältig und existieren teilweise schon seit Jahrzehnten. Sie scheinen sich jedoch inzwischen zu akkumulieren und seit 2018 dramatisch auf die Fallzahlen auszuwirken. Das Ziel, die Masern zu eliminieren, und die Aufrechterhaltung der hierfür notwendigen Impfprogramme sind ständige Herausforderungen, welche die strikte und permanente Einhaltung der WHO-Empfehlungen erfordern. Auch in Deutschland liegt die Anzahl der übermittelten Masernfälle immer noch auf einem Niveau deutlich über dem im Nationalen Impfplan festgelegten Leitziel zur Eliminierung der Masern. Immer wieder kommt es zu zeitlich begrenzten regionalen bis bundesweiten Ausbrüchen. Da Infektionserreger grenzübergreifend übertragen werden können, ist die internationale Perspektive ein wesentlicher Bestandteil der nationalen Gesundheitspolitik in Deutschland

Resurgence of Field Fever in a Temperate Country: An Epidemic of Leptospirosis among Seasonal Strawberry Harvesters in Germany in 2007

Background: Although leptospirosis is a reemerging zoonosis of global importance, outbreaks related to agricultural exposures are primarily situated in tropical countries. In July 2007, a suspected leptospirosis outbreak was recognized among strawberry harvesters from Eastern Europe who were working in Germany. An investigation was initiated to identify the outbreak source and the risk factors for infection. Methods: We conducted a retrospective cohort study with use of a questionnaire administered to harvesters by health authorities in Romania, Slovakia, and Poland. Collected serum samples were tested by microscopic agglutination test and immunoglobulin M enzyme‐linked immunosorbent assay. A case patient was defined as a person who worked in the strawberry field during the period 5 June–8 September 2007 and had leptospirosis‐compatible symptoms and either an antibody titer 1:800 and a positive immunoglobulin M enzyme‐linked immunosorbent assay result (for a confirmed case) or no serological confirmation (for a suspected case). Local rodents were examined for leptospirosis. Results: Among 153 strawberry harvesters, we detected 13 confirmed case patients who had test results positive for antibodies against Leptospira species serogroup Grippotyphosa and 11 suspected case patients (attack rate, 16%). Risk of disease increased with each day that an individual worked in the rain with hand wounds (odds ratio, 1.1; 95% confidence interval, 1.04–1.14) and accidental rodent contact (odds ratio, 4.8; 95% confidence interval, 1.5–15.9). Leptospires of the serogroup Grippotyphosa were isolated from the kidneys of 7 (64%) of 11 voles. Conclusions: This is, to our knowledge, the largest leptospirosis epidemic to occur in Germany since the 1960s. Contact between hand lesions and contaminated water or soil and infected voles was the most likely outbreak source. The unusually warm winter of 2006–2007 supported vole population growth and contributed to this resurgence of leptospirosis in Germany. Because of ongoing climate change, heightened awareness of leptospirosis in temperate regions is warranted