Opposites Do Not Attract: The Impact of Domestic Institutions, Power, and Prior Commitments on Alignment Choices

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/146950/1/0020-8833.00055.pd

Vortices within vortices: hierarchical nature of vortex tubes in turbulence

The JHU turbulence database [1] can be used with a state of the art visualisation tool [2] to generate high quality fluid dynamics videos. In this work we investigate the classical idea that smaller structures in turbulent flows, while engaged in their own internal dynamics, are advected by the larger structures. They are not advected undistorted, however. We see instead that the small scale structures are sheared and twisted by the larger scales. This illuminates the basic mechanisms of the turbulent cascade.Comment: 2 pages, 1 low quality video, 1 high quality vide

Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II

The Rpb4 and Rpb7 subunits of eukaryotic RNA polymerase II (RNAP(II)) form a heterodimer that protrudes from the 10-subunit core of the enzyme. We have obtained crystals of the human Rpb4/Rpb7 heterodimer and determined the structure to 2.7 Å resolution. The presence of putative RNA-binding domains on the Rpb7 subunit and the position of the heterodimer close to the RNA exit groove in the 12 subunit yeast polymerase complex strongly suggests a role for the heterodimer in binding and stabilizing the nascent RNA transcript. We have complemented the structural analysis with biochemical studies directed at dissecting the RNA-binding properties of the human Rpb4/Rpb7 complex and that of the homologous E/F complex from Methanocaldococcus jannaschii. A number of conserved, solvent-exposed residues in both the human Rpb7 subunit and the archaeal E subunit have been modified by site-directed mutagenesis and the mutants tested for RNA binding by performing electrophoretic mobility shift assays. These studies have identified an elongated surface region on the corresponding face of both subunit E and Rpb7 that is involved in RNA binding. The area spans the nucleic acid binding face of the OB fold, including the B4–B5 loop, but also extends towards the N-terminal domain

Increasing Tumor Volume Is Predictive of Poor Overall and Progression-Free Survival: Secondary Analysis of the Radiation Therapy Oncology Group 93-11 Phase I-II Radiation Dose-Escalation Study In Patients With Inoperable Non-Small-Cell Lung Cancer

Purpose Patients with non–small-cell lung cancer (NSCLC) in the Radiation Therapy Oncology Group (RTOG) 93-11 trial received radiation doses of 70.9, 77.4, 83.8, or 90.3 Gy. The locoregional control and survival rates were similar among the various dose levels.We investigated the effect of the gross tumor volume (GTV) on the outcome. Methods and Materials The GTV was defined as the sum of the volumes of the primary tumor and involved lymph nodes. The tumor response, median survival time (MST), and progression-free survival (PFS) were analyzed separately for smaller (≤45 cm3) vs. larger (\u3e45 cm3) tumors. Results The distribution of the GTV was as follows: ≤45 cm3 in 79 (49%) and \u3e45 cm3 in 82 (51%) of 161 patients. The median GTV was 47.3 cm3. N0 status and female gender were associated with better tumor responses. Patients with smaller (≤45 cm3) tumors achieved a longer MST and better PFS than did patients with larger (\u3e45 cm3) tumors (29.7 vs. 13.3 months, p \u3c 0.0001; and 15.8 vs. 8.3 months, p \u3c 0.0001, respectively). Increasing the radiation dose had no effect on the MST or PFS. On multivariate analysis, only a smaller GTV was a significant prognostic factor for improved MST and PFS (hazard ratio [HR], 2.12, p = 0.0002; and HR, 2.0, p = 0.0002, respectively). The GTV as a continuous variable was also significantly associated with the MST and PFS (HR, 1.59, p \u3c 0.0001; and HR, 1.39, p \u3c 0.0001, respectively). Conclusions Radiation dose escalation up to 90.3 Gy did not result in improved MST or PFS. The tumor responses were greater in node-negative patients and women. An increasing GTV was strongly associated with decreased MST and PFS. Future radiotherapy trials patients might need to use stratification by tumor volume. Int. J. Radiation Oncology Biol. Physics, Volume 70, No. 2, pp. 385-390, 200

Spitzer Infrared Observations and Independent Validation of the Transiting Super-Earth CoRoT-7b

The detection and characterization of the first transiting super-Earth, CoRoT-7 b, has required an unprecedented effort in terms of telescope time and analysis. Although the star does display a radial velocity signal at the period of the planet, this has been difficult to disentangle from the intrinsic stellar variability, and pinning down the velocity amplitude has been very challenging. As a result, the precise value of the mass of the planet - and even the extent to which it can be considered to be confirmed - have been debated in the recent literature, with six mass measurements published so far based on the same spectroscopic observations, ranging from about 2 to 8 Earth masses. Here we report on an independent validation of the planet discovery, using one of the fundamental properties of a transit signal: its achromaticity. We observed four transits of CoRoT-7 b with Spitzer, in order to determine whether the depth of the transit signal in the near-infrared is consistent with that observed in the CoRoT bandpass, as expected for a planet. We detected the transit and found an average depth of 0.426 {\pm} 0.115 mmag at 4.5 {\mu}m, which is in good agreement with the depth of 0.350 {\pm} 0.011 mmag found by CoRoT. These observations place important constraints on the kinds of astrophysical false positives that could mimic the signal. Combining this with additional constraints reported earlier, we performed an exhaustive exploration of possible blends scenarios for CoRoT-7 b using the BLENDER technique. We are able to rule out the vast majority of false positives, and the remaining ones are found to be much less likely than a true transiting planet. We thus validate CoRoT-7 b as a bona-fide planet with a very high degree of confidence, independently of any radial-velocity information. Our Spitzer observations have additionally allowed us to significantly improve the ephemeris of the planet.Comment: Accepted by Ap

A screening instrument for side dominance in competitive adolescent alpine skiers

Previous research has shown that high school ski students injure their left anterior cruciate ligament (ACL) more often than their right ACL, and that a prevention program focusing on equal load to the right and left ski turns prevents ACL injuries. Whether the injuries were in the dominant or non-dominant side of ski students was not determined but may be important knowledge to ski coaches for future design of ski-specific training programs. There is no gold standard on how to investigate the dominant side of alpine skiers. Therefore, the aim of this study was to develop a screening instrument consisting of five questions for identifying side dominance and to evaluate side dominance in competitive adolescent alpine skiers. First, 121 competitive adolescent alpine skiers answered the questions on side dominance using a test-retest design. The questions were: which hand/arm (left/right) or foot/leg (left/right) one uses as the first choice when writing, throwing, kicking a ball, jumping over a fence and stair-climbing. A question about safer/better ski turn to the left or to the right was also added. Second, 274 skiers answered the questions at one occasion. A very good agreement was shown in writing and throwing and kicking a ball, and a fair agreement was shown in jumping over a fence and stair climbing. A total of 243 skiers reported right-sided dominance, and seven skiers reported left-sided dominance. One hundred and nineteen of the 121 skiers who took part in the test-retest design answered the question safer/better ski turn, and of those 70 (59%) reported that they had a safer/better ski turn to one side than to the other side. However, the side was not consistent between the two test occasions, and the question did not correlate with side dominance. A combination of the three questions “What hand/arm do you use as first choice when writing?” “What hand/arm do you use as first choice when throwing?” and “What foot/leg do you use as first choice when kicking a ball?”, may be used to decide side dominance in adolescent alpine skiers. Most adolescent alpine skiers reported right-sided dominance