FLAG3D: A 3D Fitness Activity Dataset with Language Instruction

With the continuously thriving popularity around the world, fitness activity analytic has become an emerging research topic in computer vision. While a variety of new tasks and algorithms have been proposed recently, there are growing hunger for data resources involved in high-quality data, fine-grained labels, and diverse environments. In this paper, we present FLAG3D, a large-scale 3D fitness activity dataset with language instruction containing 180K sequences of 60 categories. FLAG3D features the following three aspects: 1) accurate and dense 3D human pose captured from advanced MoCap system to handle the complex activity and large movement, 2) detailed and professional language instruction to describe how to perform a specific activity, 3) versatile video resources from a high-tech MoCap system, rendering software, and cost-effective smartphones in natural environments. Extensive experiments and in-depth analysis show that FLAG3D contributes great research value for various challenges, such as cross-domain human action recognition, dynamic human mesh recovery, and language-guided human action generation. Our dataset and source code will be publicly available at https://andytang15.github.io/FLAG3D

MicroRNA-322 inhibits inflammatory cytokine expression and promotes cell proliferation in LPS-stimulated murine macrophages by targeting NF-κB1 (p50)

Correspondence : Hanchuan Dai ([email protected]) Inflammation is the body's normal self-protection mechanism to eliminate pathogens and resist pathogen invasion. The excessive inflammatory response may lead to inflammatory lesions. The mechanisms accounting for inflammation remain hazy. miRNAs have been proposed to have crucial effects on inflammation. In the present study, we reported that lipopolysaccharide (LPS)-stimulation increased the expression levels of inflammatory cytokines and the cell-cycle progression was suppressed in RAW264.7 cells. Meanwhile, the expression of miR-322 was significantly down-regulated after LPS treatment. Bioinformatics predictions revealed a potential binding site of miR-322 in 3 -UTR of NF-κB1 (p50) and it was further confirmed by luciferase assay. Moreover, both the mRNA and protein levels of NF-κB1 (p50) were down-regulated by miR-322 in RAW264.7 cells. Subsequently, we demonstrated that miR-322 mimics decrease in the expression levels of inflammatory cytokines and cell-cycle repression can be rescued following LPS treatment in RAW264.7 cells. The anti-inflammatory cytokines expression including IL-4 and IL-10 were significantly up-regulated. Furthermore, miR-322 could also promote RAW264.7 cells proliferation. These results demonstrate that miR-322 is a negative regulator of inflammatory response by targeting NF-κB1 (p50)

Genetic Diversity and Mating System of Two Mangrove Species (<i>Rhizophora apiculata</i> and <i>Avicennia marina</i>) in a Heavily Disturbed Area of China

Mangrove forests are distributed in the intertidal zones of tropical and subtropical regions, and have been severely damaged by anthropogenic activities, climate change, and stochastic events. Although much progress has been made in the conservation and restoration of mangroves in China, studies of the genetic diversity of mangroves are lacking, especially for isolated populations, yet such studies are essential for guiding conservation and restoration efforts. Here, we evaluated the genetic diversity, spatial genetic structure, and mating system of two mangrove species, Rhizophora apiculata and Avicennia marina, in a heavily disturbed area in Tielu Harbor, Sanya City, Hainan Island, China, using 18 nuclear microsatellite markers. We found that the genetic diversity of R. apiculata, which is classified as ‘Vulnerable’ in the China Red List categories, was high and similar compared with the genetic diversity estimates of other populations reported in previous studies. In contrast, the genetic diversity of A. marina, which is classified as a species of ‘Least Concern’, was low compared with the genetic diversity estimates of other populations. We then evaluated the presence of genetic bottlenecks, spatial genetic structure, and the mating system to determine the effects that habitat destruction has had on these two species. Our findings indicate that distinct conservation and restoration approaches are needed for these two species. Generally, our results provide valuable information that will aid the development of conservation and restoration strategies for the mangroves of Tielu Harbor

Genetic Diversity and Mating System of Two Mangrove Species (Rhizophora apiculata and Avicennia marina) in a Heavily Disturbed Area of China

Mangrove forests are distributed in the intertidal zones of tropical and subtropical regions, and have been severely damaged by anthropogenic activities, climate change, and stochastic events. Although much progress has been made in the conservation and restoration of mangroves in China, studies of the genetic diversity of mangroves are lacking, especially for isolated populations, yet such studies are essential for guiding conservation and restoration efforts. Here, we evaluated the genetic diversity, spatial genetic structure, and mating system of two mangrove species, Rhizophora apiculata and Avicennia marina, in a heavily disturbed area in Tielu Harbor, Sanya City, Hainan Island, China, using 18 nuclear microsatellite markers. We found that the genetic diversity of R. apiculata, which is classified as &lsquo;Vulnerable&rsquo; in the China Red List categories, was high and similar compared with the genetic diversity estimates of other populations reported in previous studies. In contrast, the genetic diversity of A. marina, which is classified as a species of &lsquo;Least Concern&rsquo;, was low compared with the genetic diversity estimates of other populations. We then evaluated the presence of genetic bottlenecks, spatial genetic structure, and the mating system to determine the effects that habitat destruction has had on these two species. Our findings indicate that distinct conservation and restoration approaches are needed for these two species. Generally, our results provide valuable information that will aid the development of conservation and restoration strategies for the mangroves of Tielu Harbor

ACCEPTED MANUSCRIPT MicroRNA-322 inhibits inflammatory cytokine expression and promotes cell proliferation in LPS-stimulated murine macrophages by targeting NF-κB1 (P50) BIOSCIENCE REPORTS MicroRNA-322 inhibits inflammatory cytokine expression and promote

Inflammation is the body&apos;s normal self-protection mechanism to eliminate pathogens and resist pathogen invasion. The excessive inflammatory response may lead to inflammatory lesions. The mechanisms accounting for inflammation remain hazy. MicroRNAs (miRNAs) have been proposed to have crucial effects on inflammation. In the current study, we reported that lipopolysaccharide (LPS) stimulation increases the expression levels of inflammatory cytokines and the cell cycle progression was suppressed in RAW264.7 cells. Meanwhile, the expression of miR-322 was significantly downregulated after LPS treated. Bioinformatics predictions revealed a potential binding site of miR-322 in 3′-untranslated region (3′-UTR) of and it was further confirmed by luciferase assay. Moreover, both the mRNA and protein levels of NF-κB1 (P50) were downregulated by miR-322 in RAW264.7. Subsequently, we demonstrate that miR-322 mimics decrease the expression levels of inflammatory cytokines and cell cycle repression can be rescued following LPS treated in RAW264.7 cells. The anti-inflammatory cytokines expression including IL-4 and IL-10 were significant up-regulation. Furthermore, miR-322 also could promote RAW264.7 cells proliferation. These results demonstrate that miR-322 is a negative regulator of inflammatory response by targeting NF-κB1 (P50). Abstract Inflammation is the body&apos;s normal self-protection mechanism to eliminate pathogens and resist pathogen invasion. The excessive inflammatory response may lead to inflammatory lesions. The mechanisms accounting for inflammation remain hazy. MicroRNAs (miRNAs) have been proposed to have crucial effects on inflammation. In the current study, we reported that lipopolysaccharide (LPS) stimulation increases the expression levels of inflammatory cytokines and the cell cycle progression was suppressed in RAW264.7 cells. Meanwhile, the expression of miR-322 was significantly downregulated after LPS treated. Bioinformatics predictions revealed a potential binding site of miR-322 in 3′-untranslated region (3′-UTR) of NF-κB1 (P50) and it was further confirmed by luciferase assay. Moreover, both the mRNA and protein levels of NF-κB1 (P50) were downregulated by miR-322 in RAW264.7. Subsequently, we demonstrate that miR-322 mimics decrease the expression levels of inflammatory cytokines and cell cycle repression can be rescued following LPS treated in RAW264.7 cells. The anti-inflammatory cytokines expression including IL-4 and IL-10 were significant up-regulation. Furthermore, miR-322 also could promote RAW264.7 cells proliferation. These results demonstrate that miR-322 is a negative regulator of inflammatory response by targeting NF-κB1 (P50)