Discovery of an extra hard spectral component in the high-energy afterglow emission of GRB 130427A

The extended high-energy gamma-ray (>100 MeV) emission occurred after the prompt gamma-ray bursts (GRBs) is usually characterized by a single power-law spectrum, which has been explained as the afterglow synchrotron radiation. The afterglow inverse-Compton emission has long been predicted to be able to produce a high-energy component as well, but previous observations have not revealed such a signature clearly, probably due to the small number of >10 GeV photons even for the brightest GRBs known so far. In this Letter, we report on the Fermi Large Area Telescope (LAT) observations of the >100 MeV emission from the very bright and nearby GRB 130427A. We characterize the time-resolved spectra of the GeV emission from the GRB onset to the afterglow phase. By performing time-resolved spectral fits of GRB 130427A, we found a strong evidence of an extra hard spectral component that exists in the extended high-energy emission of this GRB. We argue that this hard component may arise from the afterglow inverse Compton emission.Comment: 5 pages, 2 figures, 2 tables, ApJL, in pres

Numerical simulation analysis of multi-physical coupling field with abrasive flow precision polishing variable-aperture tube

In the fields of aerospace, precision machinery and energy, there are variable-diameter tubes for many key components. Abrasive flow polishing technology is an effective precision polishing technology for finishing the workpiece with an internal cavity structure. This technology can improve the quality of part finishing, reduce stress concentration, and reduce the surface roughness of the workpiece, and ultimately can improve the performance of the workpiece. In this paper, variable-aperture tube is taken as the research object, and the characteristics of abrasive flow in the multi-physics coupling field are studied by numerical simulation, and the collision removal effect between the abrasive particle and the wall surface is analyzed. We also discussed the control law of the abrasive grain flow processing quality and provided theoretical guidance for abrasive grain flow polishing of this type of part

Allograft function predicts mortality in kidney transplant recipients with severe COVID-19: a paradoxical risk factor

IntroductionKidney transplant recipients (KTRs) are at a higher risk of severe coronavirus disease (COVID-19) because of their immunocompromised status. However, the effect of allograft function on the prognosis of severe COVID-19 in KTRs is unclear. In this study, we aimed to analyze the correlation between pre-infection allograft function and the prognosis of severe COVID-19 in KTRs.MethodsThis retrospective cohort study included 82 patients who underwent kidney transplantation at the Sichuan Provincial Peoples Hospital between October 1, 2014 and December 1, 2022 and were diagnosed with severe COVID-19. The patients were divided into decreased eGFR and normal eGFR groups based on the allograft function before COVID-19 diagnosis (n=32 [decreased eGFR group], mean age: 43.00 years; n=50 [normal eGFR group, mean age: 41.88 years). We performed logistic regression analysis to identify risk factors for death in patients with severe COVID-19. The nomogram was used to visualize the logistic regression model results.ResultsThe mortality rate of KTRs with pre-infection allograft function insufficiency in the decreased eGFR group was significantly higher than that of KTRs in the normal eGFR group (31.25% [10/32] vs. 8.00% [4/50], P=0.006). Pre-infection allograft function insufficiency (OR=6.96, 95% CI: 1.4633.18, P=0.015) and maintenance of a mycophenolic acid dose >1500 mg/day before infection (OR=7.59, 95% CI: 1.0853.20, P=0.041) were independent risk factors, and the use of nirmatrelvir/ritonavir before severe COVID-19 (OR=0.15, 95% CI: 0.030.72, P=0.018) was a protective factor against death in severe COVID-19.ConclusionsPre-infection allograft function is a good predictor of death in patients with severe COVID-19. Allograft function was improved after treatment for severe COVID-19, which was not observed in patients with non-severe COVID-19

Identification of two novel host proteins interacting with Toxoplasma gondii 14-3-3 protein by yeast two-hybrid system

Toxoplasma gondii deploys many effector proteins in order to hijack and manipulate host cell signaling pathways, allowing parasite colonization, subversion of immune responses, and disease progression. T. gondii effector protein 14-3-3 (Tg14-3-3) promotes parasite dissemination inside the body, by enhancing the migratory ability of infected microglia and dendritic cells. Understanding both the mechanism of action and the host targets of Tg14-3-3 effector is important because of their importance to the parasite’s virulence. The aim of the present study was to explore the function of Tg14-3-3 by utilizing the yeast two-hybrid system (Y2HS) to identify novel Tg14-3-3 interactors/substrates in host cells. A human cDNA library was screened using Tg14-3-3 as the bait. Tg14-3-3 (RH strain, Type I) was cloned into the pGBKT7 vector and expressed in the Y2HGold yeast strain. The bait protein expression was validated by Western blotting analysis, auto-activation, and toxicity investigation compared with control (Y2HGold yeast strain transformed with empty pGBKT7 vector). Two positive Tg14-3-3 interactors identified by this screening, hCG1821272 and eIF5B (eukaryotic translation initiation factor 5B), were isolated and characterized. This approach made it possible to gain a better understanding of the function of Tg14-3-3 in regulating host proteins involved in key cellular processes, such as translational initiation and cell migration

Numerical simulation analysis of multi-physical coupling field with abrasive flow precision polishing variable-aperture tube

In the fields of aerospace, precision machinery and energy, there are variable-diameter tubes for many key components. Abrasive flow polishing technology is an effective precision polishing technology for finishing the workpiece with an internal cavity structure. This technology can improve the quality of part finishing, reduce stress concentration, and reduce the surface roughness of the workpiece, and ultimately can improve the performance of the workpiece. In this paper, variable-aperture tube is taken as the research object, and the characteristics of abrasive flow in the multi-physics coupling field are studied by numerical simulation, and the collision removal effect between the abrasive particle and the wall surface is analyzed. We also discussed the control law of the abrasive grain flow processing quality and provided theoretical guidance for abrasive grain flow polishing of this type of part