Transcriptome Data Revealed the circRNA–miRNA–mRNA Regulatory Network during the Proliferation and Differentiation of Myoblasts in Shitou Goose

CircRNA, a recently characterized non-coding RNA (ncRNA) variant, functions as a molecular sponge, exerting regulatory control by binding to microRNA (miRNA) and modulating the expression of downstream proteins, either promoting or inhibiting their expression. Among poultry species, geese hold significant importance, prized by consumers for their delectable taste and rich nutritional content. Despite the prominence of geese, research on the growth and development of goose muscle, particularly the regulatory role of circRNAs in goose muscle formation, remains insufficiently explored. In this study, we constructed comprehensive expression profiles of circRNAs and messenger RNAs (mRNAs) within the myoblasts and myotubes of Shitou geese. We identified a total of 96 differentially expressed circRNAs (DEcircRNAs) and 880 differentially expressed mRNAs (DEmRNAs). Notably, the parental genes of DEcircRNAs and DEmRNAs exhibited enrichment in the Wnt signaling pathway, highlighting its potential impact on the proliferation and differentiation of goose myoblasts. Employing RNAhybrid and miRDB, we identified circRNA-miRNA pairs and mRNA-miRNA pairs that may play a role in regulating myogenic differentiation or muscle growth. Subsequently, utilizing Cytoscape, we constructed a circRNA–miRNA–mRNA interaction network aimed at unraveling the intricate regulatory mechanisms involved in goose muscle growth and development, which comprises 93 circRNAs, 351 miRNAs, and 305 mRNAs. Moreover, the identification of 10 hub genes (ACTB, ACTN1, BDNF, PDGFRA, MYL1, EFNA5, MYSM1, THBS1, ITGA8, and ELN) potentially linked to myogenesis, along with the exploration of their circRNA–miRNA–hub gene regulatory axis, was also conducted. These competitive endogenous RNA (ceRNA) regulatory networks elucidate the molecular regulatory mechanisms associated with muscle growth in Shitou geese, providing deeper insights into the reciprocal regulation of circRNA, miRNA, and mRNA in the context of goose muscle formation

Tuzoia specimens studied in this paper

Tuzoia specimens studied in this pape

L-H

Bivariate graph showing size distribution of Tuzoia spp. from the Balang and Kaili biotas (T. sinensis Pan, 1957 in purple; T. lazizhaiensis sp. nov. in blue, and T. bispinosa Yuan & Zhao, 1999 in red); valve length (L) on x-axis, and valve height (H) on y-axis

Land use change on the surface area and the influence on carbon

China has diversified landforms, the three-dimensional space area check is more accurate to help determine China’s land use change and the caused carbon variations. This study explored a new method to check China’s surface area and examine the terrestrial carbon changes for the period of 2000–2020. The results show that China’s surface area increased by 13.9% compared with the planar area, with the increased area measuring 133 × 104 km2. The south and the west, especially the southwest, usually have a high area increasing rate. Woodland has the highest area increasing rate for all the provinces. 10% of the land had its land use type changed. Cropland, grassland and unused show total land area decrease, woodland, water, and impervious all increased. The mean increasing rate of land transfer on surface area varied between 1.39% and 38.84%. The total amount of land use-type change caused carbon loss reached −5907.44 × 104 t, of −3168.97 × 104 t from vegetation storage loss, −2738.77 × 104 t from NPP and water. There were only seven provinces show carbon increase, which were more located in the west. Per unit of woodland loss will cause higher carbon release than other land use types. Land use control need to be further strengthened, especially for the protection of woodland at mountain regions

The contribution of qualitative CEUS to the determination of malignancy in adnexal masses, indeterminate on conventional US - a multicenter study.

The aim of this study is to evaluate the efficacy of qualitative analysis of contrast-enhanced ultrasound (CEUS) in discrimination of adnexal masses which were undetermined by conventional ultrasound (US). A total of 120 patients underwent transabdominal CEUS. The initial enhancement time and intensity compared with the uterine myometrium, contrast agent distribution patterns and dynamic changes of enhancement were assessed. The sensitivity (Sen), specificity (Spe), positive predictive value (PPV), negative predictive value (NPV), accuracy (ACC) and Youden's index were calculated for contrast variables. The gold standard was the histological diagnosis. There were 48 malignant tumors and 72 benign tumors. The enhancement features of malignant masses were different from benign ones. Earlier or simultaneous enhancement with inhomogeneous enhancement yielded the highest capability in differential diagnosis, and Sen, Spe, PPV, NPV, ACC, Youden's index was 89.6%, 97.2%, 93.2%, 95.6%, 93.3%, and 0.88, respectively. The qualitative evaluation of CEUS is useful in the differential diagnosis of adnexal masses where conventional US is indeterminate

Long-term efficacy and safety of microwave ablation for hepatocellular carcinoma adjacent to the gallbladder with a diameter ≤ 5 cm: a multicenter, propensity score matching study

AbstractObjective To compare the long-term efficacy and safety of microwave ablation (MWA) as first-line therapy for hepatocellular carcinoma (HCC) adjacent versus nonadjacent to the gallbladder.Materials and Methods From 2006 to 2018, 657 patients with ≤5 cm HCC who underwent percutaneous ultrasound-guided MWA as first-line therapy from 5 hospitals were enrolled in this retrospective study. Patients were grouped into the adjacent group (n = 49) and the nonadjacent group (n = 608) according to whether the tumor was adjacent to the gallbladder. Propensity score matching (PSM) was used to balance baseline variables between the two groups.Results Forty-eight patient pairs were matched after PSM. For the PSM cohort, during a median follow-up time of 60 months, there were no differences in PFS (hazard ratio [HR], 1.011; 95% confidence interval [CI], 0.647-1.578; p = 0.963) or OS (HR 0.925; 95% CI 0.522-1.639; p = 0.789) between the adjacent and nonadjacent groups. Univariate and multivariate analyses revealed that the tumor adjacent to the gallbladder was not an independent risk factor for PFS or OS (all p > 0.05). Subgroup analysis showed comparable PFS and OS between the two groups in the  0.05). In addition to more use of assistive technology (p  0.05) to the nonadjacent group.Conclusion There were comparable long-term efficacy and complications between patients with HCC adjacent and nonadjacent to the gallbladder treated with MWA

The diagnostic capability of CEUS in differential diagnosis of adnexal masses.

<p>A Earlier or simultaneous beginning of enhancement; B Hyper- or iso-enhancment; C Heterogeneous enhancement.</p

A 22-year-old woman with serous cystadenocarcinoma.

<p>A: Gray-scale sonogram shows an 5.2-cm echogenic mass (arrow) with solid projection at the left of uterus. B: Color Doppler shows minimal-moderate vascularization. C: CEUS scan at 12 s shows earlier enhancement (arrow) in comparison with myometrium. D: CEUS scan at 15 s shows inhomogeneous hyper-enhancement with agent perfusion inside (arrow).</p

The contrast agent distribution patterns of the adnexal masses on CEUS.

<p>The contrast agent distribution patterns of the adnexal masses on CEUS.</p

The dynamic changes of enhancement of the adnexal masses on CEUS.

<p>The dynamic changes of enhancement of the adnexal masses on CEUS.</p