Powdery mildew responsive genes of resistant grapevine cultivar 'Regent'

The ascomycete Erysiphe necator causes powdery mildew disease of grapevine, a disastrous infection which is commonly defeated with multiple fungicide applications in viticulture. Breeding for natural resistance of quality grapes (Vitis vinifera) is thus a major aim of current efforts. The cultivar 'Regent' is resistant to powdery mildew due to an introgression from an American Vitis sp. resistance donor. To identify key regulatory elements in defense responses of 'Regent' we performed transcript analyses after challenging with E. necator inoculation in comparison with a susceptible grapevine. A set of genes selected from preliminary microarray hybridization results were investigated by RT-qPCR. The data indicate an important role of transcription factors MYB15, WRKY75, WRKY33, WRKY7, ethylene responsive transcription factors ERF2 and ERF5 as well as a CZF1/ZFAR transcripton factor in regulating the early defense when the fungus starts the interaction with its host by the formation of haustoria

In-flight scale/distortion calibration of the Hubble Space Telescope fixed-head star trackers

This paper describes an in-flight scale and distortion calibration procedure that has been developed for the Ball Aerospace Systems Division Fixed-Head Star Trackers (FHST's) used on the Hubble Space Telescope (HST). The FHST is a magnetically focused and deflected imaging sensor that is designed to track stars as faint as m(sub v) = 5.7 over an 8 degree by 8 degree field of view. Raw FHST position measurements are accurate to approximately 200 arcseconds, but this can be improved to 10-15 arcseconds by processing the raw measurements through calibration polynomials that correct for flat field, temperature intensity, and magnetic field effects. The coefficients for these polynomials were initially determined using ground test data. On HST the use of three FHST's is an integral part of the preliminary attitude update procedures required before the acquisition of guide stars for science observations. To this end, FHST-based attitude determination having single-axis errors no worse than 22 arcseconds (1 sigma) is required. In early 1991 it became evident that one of the HST FHST's was experiencing a significant change in its optical scale. By mid-1993 the size of this error had grown to a point that, if not corrected, it would correspond to a maximum position error on the order of 100 arcseconds. Subsequent investigations demonstrated that substantial, uncompensated cubic distortion effects had also developed, the maximum contribution to position errors from the cubic terms being on the order of 30 arcseconds. To ensure accurate FHST-based attitude updates, procedures have been developed to redetermine the FHST scale and distortion calibration coefficients based on in-flight data gathered during normal HST operations. These scale and distortion calibrations have proven very effective operationally, and procedures are in place to monitor FHST calibration changes on a continuing basis

Anthracnose susceptibility for grapevines with resistance loci to downy and powdery mildew in Southern Brazil

Anthracnose, downy and powdery mildew are the principal fungal diseases of grapes in tropical and subtropical regions. The pesticide active ingredients that control downy and powdery mildew diseases provide some protection against anthracnose attack. The release of varieties with resistance alleles to downy and powdery mildew results in less pesticide use that can increase anthracnose attack. Thus, the present work aimed to evaluate anthracnose susceptibility of genotypes with resistance loci to downy and powdery mildew under Southern Brazilian conditions. Genotype susceptibility was tested, as well as the influence of the environment (location and crop season) on increased susceptibility to anthracnose infection. To accomplish the objective, a trifactorial design was established that included 20 genotypes, two locations, and two crop seasons. Anthracnose incidence and severity were evaluated under natural infection in the field. Temperature around 16 °C and relative humidity at 84 % increased susceptibility to anthracnose infection compared to temperature around 20 °C and relative humidity at 75 %. All tested genotypes with resistance alleles to downy and powdery mildew presented symptoms of anthracnose. 'Baron', 'Cabernet Cortis' and 'Calardis Blanc' showed the least susceptibility to anthracnose, whereas 'Aromera', 'Felicia', 'Gf.2004-043-0004' and 'Gf.2004-043-0021' were the most susceptible and bore symptoms of anthracnose. Other genotypes showed variable susceptibility during the evaluation period, depending on environmental conditions. Overall, all interactions among the three tested factors were highly significant

Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells

Changes in the wall of seminiferous tubules in men with impaired spermatogenesis imply sterile inflammation of the testis. We tested the hypothesis that the cells forming the wall of seminiferous tubules, human testicular peritubular cells (HTPCs), orchestrate inflammatory events and that Toll like receptors (TLRs) and danger signals from the extracellular matrix (ECM) of this wall are involved. In cultured HTPCs we detected TLRs, including TLR2. A TLR-2 ligand (PAM) augmented interleukin 6 (IL-6), monocyte chemo-attractant protein-1 (MCP-1) and pentraxin 3 (PTX3) in HTPCs. The ECM-derived proteoglycan biglycan (BGN) is secreted by HTPCs and may be a TLR2-ligand at HTPCs. In support, recombinant human BGN increased PTX3, MCP-1 and IL-6 in HTPCs. Variable endogenous BGN levels in HTPCs derived from different men and differences in BGN levels in the tubular wall in infertile men were observed. In testes of a systemic mouse model for male infertility, testicular sterile inflammation and elevated estradiol (E2) levels, BGN was also elevated. Hence we studied the role of E2 in HTPCs and observed that E2 elevated the levels of BGN. The anti-estrogen ICI 182,780 blocked this action. We conclude that TLR2 and BGN contribute to sterile inflammation and infertility in man

Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells

Changes in the wall of seminiferous tubules in men with impaired spermatogenesis imply sterile inflammation of the testis. We tested the hypothesis that the cells forming the wall of seminiferous tubules, human testicular peritubular cells (HTPCs), orchestrate inflammatory events and that Toll like receptors (TLRs) and danger signals from the extracellular matrix (ECM) of this wall are involved. In cultured HTPCs we detected TLRs, including TLR2. A TLR-2 ligand (PAM) augmented interleukin 6 (IL-6), monocyte chemo-attractant protein-1 (MCP-1) and pentraxin 3 (PTX3) in HTPCs. The ECM-derived proteoglycan biglycan (BGN) is secreted by HTPCs and may be a TLR2-ligand at HTPCs. In support, recombinant human BGN increased PTX3, MCP-1 and IL-6 in HTPCs. Variable endogenous BGN levels in HTPCs derived from different men and differences in BGN levels in the tubular wall in infertile men were observed. In testes of a systemic mouse model for male infertility, testicular sterile inflammation and elevated estradiol (E2) levels, BGN was also elevated. Hence we studied the role of E2 in HTPCs and observed that E2 elevated the levels of BGN. The anti-estrogen ICI 182,780 blocked this action. We conclude that TLR2 and BGN contribute to sterile inflammation and infertility in man

Productivity and quality of 'Fuji Suprema' apple fruit in different rootstocks and growing conditions.

Planting density increasing with the use of dwarf rootstocks it is a fact that has changed the scenario of the pome culture in Brazil. The objective of this work was to evaluate the plant growth, productivity and fruit quality of the Fuji Suprema cultivar grafted on the rootstocks G.213 and M.9 in a new area (virgin soil) and in the replanting area of apple trees in Vacaria City in Rio Grande do Sul state, Brazil. The cultivar Fuji Suprema grafted on the rootstocks G.213 and M.9 was used, being the experiment 1 in new area and the experiment 2 in area of replanting of apple trees. In both experiments, the orchard was implanted in 2014. The spacing adopted was 4.0 m between rows and 0.9 m between plants, totaling a density of 2,777 plants per hectare. Vegetative/productive evaluations were carried out in the 2014, 2015, 2016, 2017, 2018 and 2019 harvests in commercial orchards of the company Rasip Agro Pastoril S/A. In the experiment 1, the G.213 rootstock was highlighted for the variables accumulated productivity, accumulated productive efficiency, ASTT, number of branches and scion volume. In this same experiment, the M.9 rootstock was overestimated in some crops for the variables fruit mass and pulp firmness. In the experiment 2, the G.213 rootstock was highlighted as to the accumulated productivity, accumulated productive efficiency, ASTT, number of branches and scion volume. In the 2016 crop, the rootstock M.9, stood out for the diameter and mass of fruits and soluble solids and in the 2017 harvest for pulp firmness. In order to cultivate Fuji Suprema, both in the new area and in the replanting area of apple trees, the G.213 rootstock is a new dwarf rootstock option for the southern region of Brazil

The interplay between hormone signaling and defense gene expression in grapevine genotypes carrying genetic resistance against Plasmopara viticola

The present study aimed to investigate plant defense related pathways during Plasmopara viticola infection in Vitis vinifera varieties. Plant material consisted of 'Chardonnay' (no Rpv), 'Regent' (Rpv3-1), 'Bronner' (Rpv3-3+Rpv10), 'Calardis Blanc' (Rpv3-1+Rpv3-2), and the breeding selection GF15 (Rpv1+Rpv3-1). Gene expression analysis was carried out for the varieties 'Regent', GF15, 'Bronner', and 'Chardonnay'. Hormonal quantification was performed for jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA), indole-3-acetic acid (IAA), and trans-zeatin-ribose (tZR). The samples were collected from plants cultivated in vitro inoculated with Plasmopara viticola sporangia, and collected at 0, 1-, 3-, 5-, and 7-days post inoculation (DPI) for gene expression; and 0, 3, 5, and 7 DPI for hormonal quantification. The results showed an interaction between genotype and time post inoculation in gene expression and hormonal pathways linked with pathogen recognition. Both jasmonate and salicylic acids were involved in the resistance response. The role of stilbenes acting against the pathogen at different times was also confirmed. Changes in the expression of genes linked to cell defense were observed in all evaluated genotypes; however, genotypes with R-loci responded more quickly than the variety without R-loci, activating mechanisms of cell death, resulting in symptoms of hypersensitivity

Possible pseudogap behavior of electron doped high-temperature superconductors

We have measured the low-energy quasiparticle excitation spectrum of the electron doped high-temperature superconductors (HTS) Nd(1.85)Ce(0.15)CuO(4-y) and Pr(1.85)Ce(0.15)CuO(4-y) as a function of temperature and applied magnetic field using tunneling spectroscopy. At zero magnetic field, for these optimum doped samples no excitation gap is observed in the tunneling spectra above the transition temperature Tc. In contrast, below Tc for applied magnetic fields well above the resistively determined upper critical field, a clear excitation gap at the Fermi level is found which is comparable to the superconducting energy gap below Tc. Possible interpretations of this observation are the existence of a normal state pseudogap in the electron doped HTS or the existence of a spatially non-uniform superconducting state.Comment: 4 pages, 4 ps-figures included, to be published in Phys. Rev. B, Rapid Com