1,701 research outputs found
Stochastic modeling of cargo transport by teams of molecular motors
Many different types of cellular cargos are transported bidirectionally along
microtubules by teams of molecular motors. The motion of this cargo-motors
system has been experimentally characterized in vivo as processive with rather
persistent directionality. Different theoretical approaches have been suggested
in order to explore the origin of this kind of motion. An effective theoretical
approach, introduced by M\"uller et al., describes the cargo dynamics as a
tug-of-war between different kinds of motors. An alternative approach has been
suggested recently by Kunwar et al., who considered the coupling between motor
and cargo in more detail. Based on this framework we introduce a model
considering single motor positions which we propagate in continuous time.
Furthermore, we analyze the possible influence of the discrete time update
schemes used in previous publications on the system's dynamic.Comment: Cenference proceedings - Traffic and Granular Flow 1
The role of microtubule movement in bidirectional organelle transport
We study the role of microtubule movement in bidirectional organelle
transport in Drosophila S2 cells and show that EGFP-tagged peroxisomes in cells
serve as sensitive probes of motor induced, noisy cytoskeletal motions.
Multiple peroxisomes move in unison over large time windows and show
correlations with microtubule tip positions, indicating rapid microtubule
fluctuations in the longitudinal direction. We report the first high-resolution
measurement of longitudinal microtubule fluctuations performed by tracing such
pairs of co-moving peroxisomes. The resulting picture shows that
motor-dependent longitudinal microtubule oscillations contribute significantly
to cargo movement along microtubules. Thus, contrary to the conventional view,
organelle transport cannot be described solely in terms of cargo movement along
stationary microtubule tracks, but instead includes a strong contribution from
the movement of the tracks.Comment: 24 pages, 5 figure
Advances in the prevention, management, and treatment of community-acquired pneumonia
Despite the availability of powerful antibiotics, community-acquired pneumonia (CAP) remains one of the leading reasons for morbidity and mortality worldwide, and despite the availability of powerful antibiotics, there has been only little improvement in case fatality rates for many years. Consequently, it cannot be expected that novel antibiotics will substantially improve outcomes in CAP. Therefore, this review focuses on novel approaches that may reduce CAP-related mortality: the impact of immunomodulation by macrolides and fluoroquinolones and the prevention of CAP by pneumococcal vaccines
Current thinking and new paradigm for COPD
AbstractDuring the 2015 European Respiratory Society Congress, a symposium was held on ‘Current thinking and new paradigm for COPD’. Through a combination of plenary lectures and interactive panel discussions, experts discussed the recent evidence for chronic obstructive pulmonary disease (COPD) treatment and how this evidence can be applied in clinical practice
Tuberculosis in a South African prison – a transmission modelling analysis
Background. Prisons are recognised internationally as institutions with very high tuberculosis (TB) burdens where transmission is predominantly determined by contact between infectious and susceptible prisoners. A recent South African court case described the conditions under which prisoners awaiting trial were kept. With the use of these data, a mathematical model was developed to explore the interactions between incarceration conditions and TB control measures.Methods. Cell dimensions, cell occupancy, lock-up time, TB incidence and treatment delays were derived from court evidence and judicial reports. Using the Wells-Riley equation and probability analyses of contact between prisoners, we estimated the current TB transmission probability within prison cells, and estimated transmission probabilities of improved levels of case finding in combination with implementation of national and international minimum standards for incarceration.Results. Levels of overcrowding (230%) in communal cells and poor TB case finding result in annual TB transmission risks of 90% per annum. Implementing current national or international cell occupancy recommendations would reduce TB transmission probabilities by 30% and 50%, respectively. Improved passive case finding, modest ventilation increase or decreased lock-up time would minimally impact on transmission if introduced individually. However, active case finding together with implementation of minimum national and international standards of incarceration could reduce transmission by 50% and 94%, respectively.Conclusions. Current conditions of detention for awaiting trial prisoners are highly conducive for spread of drug-sensitive and drug-resistant TB. Combinations of simple well-established scientific control measures should be implemented urgently.S Afr Med J 2011;101:809-813
Cancer stem cells in solid tumors: elusive or illusive?
During the past years in vivo transplantation experiments and in vitro colony-forming assays indicated that tumors arise only from rare cells. These cells were shown to bear self-renewal capacities and the ability to recapitulate all cell types within an individual tumor. Due to their phenotypic resemblance to normal stem cells, the term "cancer stem cells" is used. However, some pieces of the puzzle are missing: (a) a stringent definition of cancer stem cells in solid tumors (b) specific markers that only target cells that meet the criteria for a cancer stem cell in a certain type of tumor. These missing parts started an ongoing debate about which is the best method to identify and characterize cancer stem cells, or even if their mere existence is just an artifact caused by the experimental procedures. Recent findings query the cancer stem cell hypothesis for solid tumors itself since it was shown in xenograft transplantation experiments that under appropriate conditions tumor-initiating cells are not rare
Tuberculosis in a South African prison - a transmission modelling analysis
Background. Prisons are recognised internationally as institutions with very high tuberculosis (TB) burdens where transmission is predominantly determined by contact between infectious and susceptible prisoners. A. recent South African court case described the conditions under which prisoners awaiting trial were kept. With the use of these data, a mathematical model was developed to explore the interactions between incarceration conditions and TB control measures. Methods. Cell dimensions, cell occupancy, lock-up time, TB incidence and treatment delays were derived from court evidence and judicial reports. Using the Wells-Riley equation and probability analyses of contact between prisoners, we estimated the current TB transmission probability within prison cells, and estimated transmission probabilities of improved levels of case finding in combination with implementation of national and international minimum standards for incarceration. Results. Levels of overcrowding (230%) in communal cells and Poor TB case finding result in annual TB transmission risks of 90% per annum. Implementing current national or international cell occupancy recommendations would reduce TB transmission probabilities by 30% and 50%, respectively. Improved passive case finding, modest ventilation increase or decreased lock-up time would minimally impact on transmission if introduced individually. However, active case finding together with implementation of minimum national and international standards of incarceration could reduce transmission by 50% and 94%, respectively. Conclusions. Current conditions of detention for awaiting-trial prisoners are highly conducive for spread of drug-sensitive and drug-resistant TB. Combinations of simple well-established scientific control measures should be implemented urgently
Oncogenic Foxl2 is a chromatin-remodeling pioneer transcription factor in adult-type ovarian granulosa cell tumors
View full abstracthttps://openworks.mdanderson.org/leading-edge/1008/thumbnail.jp
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