Mahaska County livestock manure/crop nutrient management demonstration project

The project was developed from the requests of Mahaska County producers for information related to management of their livestock systems. Through surveys, sampling, and demonstrations, it was learned that there is a continuing need for individualized consulting linking manure and nutrient management. Producers also want more guidance about residue management and consistency of manure nutrient content

Crystal structure of the yellow 1:2 molecular complex lumiflavin–bisnaphthalene-2,3-diol

In the first molecular complex of the physiologically active neutral form of isoalloxazine studied, lumiflavin–bisnaphthalene-2,3-diol, each flavin is sandwiched between two naphthalenediol molecules with extensive overlap but a moderately large (3·44 Å) spacing, indicating at most weak charge-transfer interaction and in agreement with the yellow colour of the complex, nearly the same as that of the parent lumiflavin

Prospectus, December 15, 1977

LETTERS TO THE EDITORS: SLACK ANNOUNCES FOR STATE REP., DO THE STUDENTS KNOW?; Finals schedule revised; Star Wars cashes in on Sci-Fi movie craze; Nutcracker danced by National Ballet; Pre-school kids know why they have Christmas; Close encounters of a red and green kind; What else is new: Drivers bad?; Accidents up with sales: Experts: beware of chain saw dangers; ...and speaking of chain saws: America finds new fuel...wood; U. of I. art at krannert; A Prairie Winter: \u27Whose woods these are...\u27; Carl Sandburg: 100 new poems published; Guest artist\u27s choreography entertaining, full of surprises; Bach backers have cause to rejoice; Dirty heating equipment can make your gas bill skyrocket; Physical Science Division entices hi-schoolers with freebees, rap sessions, counseling; Santa\u27s psyche probed by his errant elves; Who was that Santa I saw you with last night?; Settlers thought Illinois land was worthless; New cases for C-U city police: parent abuse; Goodbye, yellow notched card: Computerized circulation at UI Library; Humane Society still has puppies, kittens for gifts; Life doesn\u27t end at 40, either: Seniors Center fights \u27mope at home syndrome\u27; Thimbles and Threads: home-made surprises; Book review: Elvis-What Happened?; New studies of cholesterol: Eggs not villainous after all??; Warfield, Madrigals in UI holiday broadcast; Poet\u27s Corner: The Flute; Allerton Park: As the leaves fall, the people leave; Some assistants please: The Legends of Lawrenceville; Meanwhile, back in the Cobra\u27s camp...; Greatest names of golf fame ready themselves for tourney; Former Parkland student now high school assistant coach; Home run hero hits twin towns; Classifieds; Can Illinois challenge Northwestern?: Mad challenge for NCAA supremacy; Snow stalls area basketball but not for long in Illinois; Weather, Freddy bury Bob in snow; Cobras to play tough teams during break; \u27He doesn\u27t seem big to me\u27: Student has special Cowboy Additional material: Edition 3 of the Parkland literary magazinehttps://spark.parkland.edu/prospectus_1977/1000/thumbnail.jp

Rapid cryopreservation of five mammalian and one mosquito cell line at -80°C while attached to flasks in a serum free cryopreservative

Cell culturing, and the requisite storage of cell lines at ultra-low temperatures, is used in most laboratories studying or using eukaryotic proteomics, genomics, microarray, and RNA technologies. In this study we have observed that A72(dog), CRFK(cat), NB324K(human), MCF7(human), WI38(human), and C636(mosquito) cells were effectively cryopreserved at -80°C while attached to the substratum of 25cm(2 )tissue culture flasks. This was accomplished using a serum free crypreservative recently developed by Corsini and co-workers. The technique allows for significant savings of time and money in laboratories that rapidly process numerous cell lines

Sound and Complete Typing for lambda-mu

In this paper we define intersection and union type assignment for Parigot's calculus lambda-mu. We show that this notion is complete (i.e. closed under subject-expansion), and show also that it is sound (i.e. closed under subject-reduction). This implies that this notion of intersection-union type assignment is suitable to define a semantics.Comment: In Proceedings ITRS 2010, arXiv:1101.410

The Long-Baseline Neutrino Experiment: Exploring Fundamental Symmetries of the Universe

The preponderance of matter over antimatter in the early Universe, the dynamics of the supernova bursts that produced the heavy elements necessary for life and whether protons eventually decay --- these mysteries at the forefront of particle physics and astrophysics are key to understanding the early evolution of our Universe, its current state and its eventual fate. The Long-Baseline Neutrino Experiment (LBNE) represents an extensively developed plan for a world-class experiment dedicated to addressing these questions. LBNE is conceived around three central components: (1) a new, high-intensity neutrino source generated from a megawatt-class proton accelerator at Fermi National Accelerator Laboratory, (2) a near neutrino detector just downstream of the source, and (3) a massive liquid argon time-projection chamber deployed as a far detector deep underground at the Sanford Underground Research Facility. This facility, located at the site of the former Homestake Mine in Lead, South Dakota, is approximately 1,300 km from the neutrino source at Fermilab -- a distance (baseline) that delivers optimal sensitivity to neutrino charge-parity symmetry violation and mass ordering effects. This ambitious yet cost-effective design incorporates scalability and flexibility and can accommodate a variety of upgrades and contributions. With its exceptional combination of experimental configuration, technical capabilities, and potential for transformative discoveries, LBNE promises to be a vital facility for the field of particle physics worldwide, providing physicists from around the globe with opportunities to collaborate in a twenty to thirty year program of exciting science. In this document we provide a comprehensive overview of LBNE's scientific objectives, its place in the landscape of neutrino physics worldwide, the technologies it will incorporate and the capabilities it will possess.Comment: Major update of previous version. This is the reference document for LBNE science program and current status. Chapters 1, 3, and 9 provide a comprehensive overview of LBNE's scientific objectives, its place in the landscape of neutrino physics worldwide, the technologies it will incorporate and the capabilities it will possess. 288 pages, 116 figure

Calculating total health service utilisation and costs from routinely collected electronic health records using the example of patients with irritable bowel syndrome before and after their first gastroenterology appointment

INTRODUCTION: Health economic models are increasingly important in funding decisions but most are based on data, which may therefore not represent the general population. We sought to establish the potential of real-world data available within the Clinical Practice Research Datalink (CPRD) and linked Hospital Episode Statistics (HES) to determine comprehensive healthcare utilisation and costs as input variables for economic modelling. METHODS: A cohort of patients with irritable bowel syndrome (IBS) who first saw a gastroenterologist in 2008 or 2009, and with 3 years of data before and after their appointment, was created in the CPRD. Primary care, outpatient, inpatient, prescription and colonoscopy data were extracted from the linked CPRD and HES. The appropriate cost to the NHS was attached to each event. Total and stratified annual healthcare utilisation rates and costs were calculated before and after the gastroenterology appointment with distribution parameters. Absolute differences were calculated with 95 % confidence intervals. RESULTS: Total annual healthcare costs over 3 years increase by £935 (95 % CI £928–941) following a gastroenterology appointment for IBS. We derived utilisation and cost data with parameter distributions stratified by demographics and time. Women, older patients, smokers and patients with greater comorbidity utilised more healthcare resources, which generated higher costs. CONCLUSIONS: These linked datasets provide comprehensive primary and secondary care data for large numbers of patients, which allows stratification of outcomes. It is possible to derive input parameters appropriate for economic models and their distributions directly from the population of interest

Curcumin-Arteether Combination Therapy of Plasmodium berghei-Infected Mice Prevents Recrudescence Through Immunomodulation

Earlier studies in this laboratory have shown the potential of artemisinin-curcumin combination therapy in experimental malaria. In a parasite recrudescence model in mice infected with Plasmodium berghei (ANKA), a single dose of alpha,beta-arteether (ART) with three oral doses of curcumin prevented recrudescence, providing almost 95% protection. The parasites were completely cleared in blood with ART-alone (AE) or ART+curcumin (AC) treatments in the short-term, although the clearance was faster in the latter case involving increased ROS generation. But, parasites in liver and spleen were not cleared in AE or AC treatments, perhaps, serving as a reservoir for recrudescence. Parasitemia in blood reached up to 60% in AE-treated mice during the recrudescence phase, leading to death of animals. A transient increase of up to 2–3% parasitemia was observed in AC-treatment, leading to protection and reversal of splenomegaly. A striking increase in spleen mRNA levels for TLR2, IL-10 and IgG-subclass antibodies but a decrease in those for INFγ and IL-12 was observed in AC-treatment. There was a striking increase in IL-10 and IgG subclass antibody levels but a decrease in INFγ levels in sera leading to protection against recrudescence. AC-treatment failed to protect against recrudescence in TLR2−/− and IL-10−/− animals. IL-10 injection to AE-treated wild type mice and AC-treated TLR2−/− mice was able to prolong survival. Blood from the recrudescence phase in AE-treatment, but not from AC-treatment, was able to reinfect and kill naïve animals. Sera from the recrudescence phase of AC-treated animals reacted with several parasite proteins compared to that from AE-treated animals. It is proposed that activation of TLR2-mediated innate immune response leading to enhanced IL-10 production and generation of anti-parasite antibodies contribute to protective immunity in AC-treated mice. These results indicate a potential for curcumin-based combination therapy to be tested for prevention of recrudescence in falciparum and relapse in vivax malaria

Molecular Predictors of 3D Morphogenesis by Breast Cancer Cell Lines in 3D Culture

Correlative analysis of molecular markers with phenotypic signatures is the simplest model for hypothesis generation. In this paper, a panel of 24 breast cell lines was grown in 3D culture, their morphology was imaged through phase contrast microscopy, and computational methods were developed to segment and represent each colony at multiple dimensions. Subsequently, subpopulations from these morphological responses were identified through consensus clustering to reveal three clusters of round, grape-like, and stellate phenotypes. In some cases, cell lines with particular pathobiological phenotypes clustered together (e.g., ERBB2 amplified cell lines sharing the same morphometric properties as the grape-like phenotype). Next, associations with molecular features were realized through (i) differential analysis within each morphological cluster, and (ii) regression analysis across the entire panel of cell lines. In both cases, the dominant genes that are predictive of the morphological signatures were identified. Specifically, PPARγ has been associated with the invasive stellate morphological phenotype, which corresponds to triple-negative pathobiology. PPARγ has been validated through two supporting biological assays