601 research outputs found
Immunoadsorbents - Preparation and use of cellulose derivatives
Antibody isolation from blood by linking antigens to insoluble cellulose derivative
The chemistry and use of cellulose derivatives for the study of biological systems
Cellulose chemistry and applications as carrier for proteins, polymers, and organic molecule
Metered oxygen supply aids treatment of domestic sewage
Microbiological fixed-bed process was developed in which supplementary oxygen required by microbial species is supplied by electrochemical device. Rate of addition of oxygen to waste treatment process is controlled to maintain aerobic metabolism and prevent anaerobic metabolisms which produce odorous or toxic products
Reusable chelating resins concentrate metal ions from highly dilute solutions
Column chromatographic method uses new metal chelating resins for recovering heavy-metal ions from highly dilute solutions. The absorbed heavy-metal cations may be removed from the chelating resins by acid or base washes. The resins are reusable after the washes are completed
A New Understanding of Antibiotic Action via Solid-State NMR of Cells with Uniform Isotopic Labeling
Kinetic and Dynamic Delaunay tetrahedralizations in three dimensions
We describe the implementation of algorithms to construct and maintain
three-dimensional dynamic Delaunay triangulations with kinetic vertices using a
three-simplex data structure. The code is capable of constructing the geometric
dual, the Voronoi or Dirichlet tessellation. Initially, a given list of points
is triangulated. Time evolution of the triangulation is not only governed by
kinetic vertices but also by a changing number of vertices. We use
three-dimensional simplex flip algorithms, a stochastic visibility walk
algorithm for point location and in addition, we propose a new simple method of
deleting vertices from an existing three-dimensional Delaunay triangulation
while maintaining the Delaunay property. The dual Dirichlet tessellation can be
used to solve differential equations on an irregular grid, to define partitions
in cell tissue simulations, for collision detection etc.Comment: 29 pg (preprint), 12 figures, 1 table Title changed (mainly
nomenclature), referee suggestions included, typos corrected, bibliography
update
A Mammalian Homolog of Drosophila melanogaster Transcriptional Coactivator Intersex Is a Subunit of the Mammalian Mediator Complex
The multiprotein Mediator complex is a coactivator required for transcriptional activation of RNA polymerase II transcribed genes by DNA binding transcription factors. We previously partially purified a Med8-containing Mediator complex from rat liver nuclei (Brower, C. S., Sato, S., Tomomori-Sato, C., Kamura, T., Pause, A., Stearman, R., Klausner, R. D., Malik, S., Lane, W. S., Sorokina, I., Roeder, R. G., Conaway, J. W., and Conaway, R. C. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 10353–10358). Analysis of proteins present in the most highly enriched Mediator fractions by tandem mass spectrometry led to the identification of several new mammalian Mediator subunits, as well as several potential Mediator subunits. Here we identify one of these proteins, encoded by the previously uncharacterized AK000411 open reading frame, as a new subunit of the mammalian Mediator complex. The AK000411 protein, which we designate hIntersex (human Intersex), shares significant sequence similarity with the Drosophila melanogaster intersex protein, which has functional properties expected of a transcriptional coactivator specific for the Drosophila doublesex transactivator. In addition, we show that hIntersex assembles into a subcomplex with Mediator subunits p28b and TRFP. Taken together, our findings identify a new subunit of the mammalian Mediator and shed new light on the architecture of the mammalian Mediator complex
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