156 research outputs found
Symbionts protect aphids from parasitic wasps by attenuating herbivore-induced plant volatiles
Plants respond to insect attack by releasing blends of volatile chemicals that attract their herbivores' specific natural enemies, while insect herbivores may carry endosymbiotic microorganisms that directly improve herbivore survival after natural enemy attack. Here we demonstrate that the two phenomena can be linked. Plants fed upon by pea aphids release volatiles that attract parasitic wasps, and the pea aphid can carry facultative endosymbiotic bacteria that prevent the development of the parasitic wasp larva and thus markedly improve aphid survival after wasp attack. We show that these endosymbionts also attenuate the systemic release of volatiles by plants after aphid attack, reducing parasitic wasp recruitment and increasing aphid fitness. Our results reveal a novel mechanism through which symbionts can benefit their hosts and emphasise the importance of considering the microbiome in understanding insect ecological interactions. (Résumé d'auteur
Limited effects of plant-beneficial fungi on plant volatile composition and host-choice behavior of Nesidiocoris tenuis
Biological control using plant-beneficial fungi has gained considerable interest as a sustainable method for pest management, by priming the plant for enhanced defense against pathogens and insect herbivores. However, despite promising outcomes, little is known about how different fungal strains mediate these beneficial effects. In this study, we evaluated whether inoculation of tomato seeds with the plant-beneficial fungi Beauveria bassiana ARSEF 3097, Metarhizium brunneum ARSEF 1095 and Trichoderma harzianum T22 affected the plant’s volatile organic compound (VOC) profile and the host-choice behavior of Nesidiocoris tenuis, an emerging pest species in NW-European tomato cultivation, and the related zoophytophagous biocontrol agent Macrolophus pygmaeus. Results indicated that fungal inoculation did not significantly alter the VOC composition of tomato plants. However, in a two-choice cage assay where female insects were given the option to select between control plants and fungus-inoculated plants, N. tenuis preferred control plants over M. brunneum-inoculated plants. Nearly 72% of all N. tenuis individuals tested chose the control treatment. In all other combinations tested, no significant differences were found for none of the insects. We conclude that inoculation of tomato with plant-beneficial fungi had limited effects on plant volatile composition and host-choice behavior of insects. However, the observation that N. tenuis was deterred from the crop when inoculated with M. brunneum and attracted to non-inoculated plants may provide new opportunities for future biocontrol based on a push-pull strategy
Virulence Factors of Geminivirus Interact with MYC2 to Subvert Plant Resistance and Promote Vector Performance
Herbivore-Mediated Effects of Glucosinolates on Different Natural Enemies of a Specialist Aphid
The cabbage aphid Brevicoryne brassicae is a specialist herbivore that sequesters glucosinolates from its host plant as a defense against its predators. It is unknown to what extent parasitoids are affected by this sequestration. We investigated herbivore-mediated effects of glucosinolates on the parasitoid wasp Diaeretiella rapae and the predator Episyrphus balteatus. We reared B. brassicae on three ecotypes of Arabidopsis thaliana that differ in glucosinolate content and on one genetically transformed line with modified concentrations of aliphatic glucosinolates. We tested aphid performance and the performance and behavior of both natural enemies. We correlated this with phloem and aphid glucosinolate concentrations and emission of volatiles. Brevicoryne brassicae performance correlated positively with concentrations of both aliphatic and indole glucosinolates in the phloem. Aphids selectively sequestered glucosinolates. Glucosinolate concentration in B. brassicae correlated negatively with performance of the predator, but positively with performance of the parasitoid, possibly because the aphids with the highest glucosinolate concentrations had a higher body weight. Both natural enemies showed a positive performance-preference correlation. The predator preferred the ecotype with the lowest emission of volatile glucosinolate breakdown products in each test combination, whereas the parasitoid wasp preferred the A. thaliana ecotype with the highest emission of these volatiles. The study shows that there are differential herbivore-mediated effects of glucosinolates on a predator and a parasitoid of a specialist aphid that selectively sequesters glucosinolates from its host plant
Optimization of the HS-SPME-GC/MS technique for determining volatile compounds in red wines made from Isabel grapes (Vitis labrusca)
Does Aphid Infestation Interfere with Indirect Plant Defense against Lepidopteran Caterpillars in Wild Cabbage?
How plants handle multiple stresses: hormonal interactions underlying responses to abiotic stress and insect herbivory
Analysis of organochloro-pesticides in Eritrean water and sediment samples
Thesis (MSc)--Stellenbosch University, 2004.ENGLISH ABSTRACT: The choice of the sample preparation technique used in combination with a
chromatographic technique in environmental analysis strongly influences the
performance of the method as a whole. The main aim of this work was to evaluate
methods like: micro liquid-liquid extraction (цLLE)and solid phase micro extraction
(SPME) in combination with gas chromatography-electron capture detector (GCECD)
and a recently developed technique named stir bar sorptive extraction (SBSE)
combined with thermal desorption system-programmable temperature vaporizationgas
chromatography-mass spectrometer (TDS-PTV-GC-MS) for the analysis of
organochloro-pesticides (OCPs) in natural water and to make a comparison between
them. The most suitable method was then selected for the analysis of water samples
collected from Eritrea. An additional goal was to develop a sensitive method for the
analysis ofOCPs in soil and sediment samples. цLLE-GC-ECD and SPME-GC-ECD methods were evaluated for the analysis of
OCPs in water. The former allowed achieving a sensitivity of less than 100 ppt and
the latter was able to detect down to the 1 ppt level. Both methods were found to be
repeatable with %RSD of3% to 19% and 5% to 12% as well as linear with a range of
1:103 and 1:5x103 respectivey. SBSE-TDS-GC-MS was also investigated for the
analysis of OCPs in water samples. With this technique a good sensitivity down to 1
ppt was also obtained. The less sensitive nature of the MS compared to ECD was
hereby compensated for by the large amount of polydimethylsiloxane (PDMS) phase
on the stir bar. The method was repeatable and linear with a range of 1:5000, which is
similar to the one obtained for the SPME-GC-ECD method. All three methods were
also tested with real contaminated water samples and a comparison of the three
techniques in terms of sensitivity, linearity, repeatability, availability and cost
effectiveness was done. As a method of choice in terms of the above criteria, SPMEGC-
ECD was applied to the water samples collected from Eritrea. The presence of
some oePs such as a-BBC (benzenehexachloride), heptachlor, heptachlor epoxide,
endosulfan I, p,p'-DDE (dichlorodiphenylchloroethylene), endosulfan II, p,p'-DDD
(dichlorodiphenyldichloroethane), endosu!fan sulfate and p,p'-DDT
(dichlorodiphenyltrichloroethane) in some of the Eritrean water samples was
demonstrated The SPME-GC-ECD technique for the analysis OCPs in soil and sediment samples
was investigated. It was shown to be able to detect down to 1 pg/g (lppt) with good
linearity and repeatability. The method was also evaluated for authentic soil samples.
Due to a lack of time the method could not be applied for the analysis of the soil and
sediment samples collected from Eritrea.AFRIKAANSE OPSOMMING: Die keuse van 'n gepaste monster-voorbereidingsmetode wat gebruik word tesame
met 'n chromatografiese skeidingtegniek in omgewings-analise het 'n beduidende
effek op die algehele metode. Die doel van hierdie werk was om metodes soos mikrovloeistof-
vloeistof ekstraksie (цLLE) en soliede fase mikro ekstraksie (SPME) in
kombinasie met gaschromatografie met elektronvangs deteksie (GC-ECD) te
ontwikkel vir die analise van organochloor-pestisiede (OCPs) in natuurlike water.
Vervolgens is hierdie metodes vergelyk met die onlangs-ontwikkelde roerstaaf
sorptiewe ekstraksie tegniek (SBSE) in kombinasie met termiese desorbsie-GCmassaspektrometriese
deteksie (TDS-GC-MSD). Die mees geskikte metode is
vervolgens gebruik vir die analise van Eritreaanse watermonsters. Daarbenewens was
die doelook om 'n sensitiewe metode te ontwikkel vir die analise van OCPs in gronden
sedimentmonsters.
Beide цLLE en SPME-GC-ECD metodes is gekarakteriseer deur goeie
herhaalbaarheid en lineariteit. Die sensitiwiteit van bogenoemde metodes was minder
as 100 dele per triljoen (ppt) en 1 ppt, respektiewelik. Ook met SBSE-TDS-GC-MSD
kon 'n deteksielimiet van 1 ppt bereik word. In hierdie geval is vir die verlies van
sensitiwiteit van die MSD in vergelyking met die ECD, gekompenseer deur die groter
hoeveelheid PDMS op die roerstaaf in vergelyking met 'n SPME apparaat. Die
herhaalbaarheid en lineariteit van die metode is vergelykbaar met dié van die SPMEGC-
ECD metode.
Al drie metodes is vergelyk vir die analise van gekontamineerde watermonsters in
terme van sensitiwiteit, liniêre bereik, herhaalbaarheid, toeganklikheid en kosteeffektiwiteit.
SPME-GC-ECD is vervolgens gekies as metode van voorkeur vir die
analise van Eritreaanse watermonsters. Die teenwoordigheid van sekere OCPs soos
a-BHC, heptachloor, heptachloorepoksied, endosulfan I, p,p'-DDE, endosulfan II,
p,p' -DDD, endosulfan sulfaat en p,p' -DDT in die monsters is gedemonstreer.
Die toepasbaarheid van die SPME-GC-ECD metode vir die analise van OCPs in
grond-en sedimentmonsters is ook geëvalueer, en dit was moontlik om so min as 1
pg/g waar te neem. Weens tydbeperkings was dit nie moontlik om Eritreaanse
grondmonsters met die ontwikkelde metode te analiseer nie
Analysis of volatiles in Pinotage wines by stir bar sorptive extraction and chemometric profiling
A fast, simple, cost-effective, and reliable method based on stir bar sorptive extraction (SBSE) in the headspace mode was used for the analysis of 39 volatile components in Pinotage wines. The method was sensitive, with LODs ranging from 50.0 pg/L to 281 ng/L and LOQs between 180 pg/L and 938 ng/L. Precision was between 6 and 20%. The intermediate precision was within the acceptable range. Moreover, good calibration curves with R2 > 0.99 for all compounds were achieved. The method was successfully applied for the analysis of 87 young Pinotage wines of vintages 2005 and 2006 collected from various South African regions. To characterize the results based on vintage and origin, the obtained concentrations of the compounds were subjected to chemometric analysis. Exploratory factor analysis (FA), principal component analysis (PCA), and analysis of variance (one-way ANOVA) were consecutively done. The chemometrics approach revealed a reasonable correlation among the volatile components of these wines, as well as with respect to their year of production. © 2008 American Chemical Society.Articl
Analysis of volatiles in Pinotage wines by stir bar sorptive extraction and chemometric profiling
A fast, simple, cost-effective, and reliable method based on stir bar sorptive extraction (SBSE) in the headspace mode was used for the analysis of 39 volatile components in Pinotage wines. The method was sensitive, with LODs ranging from 50.0 pg/L to 281 ng/L and LOQs between 180 pg/L and 938 ng/L. Precision was between 6 and 20%. The intermediate precision was within the acceptable range. Moreover, good calibration curves with R2 > 0.99 for all compounds were achieved. The method was successfully applied for the analysis of 87 young Pinotage wines of vintages 2005 and 2006 collected from various South African regions. To characterize the results based on vintage and origin, the obtained concentrations of the compounds were subjected to chemometric analysis. Exploratory factor analysis (FA), principal component analysis (PCA), and analysis of variance (one-way ANOVA) were consecutively done. The chemometrics approach revealed a reasonable correlation among the volatile components of these wines, as well as with respect to their year of production. © 2008 American Chemical Society.Articl
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