2,872 research outputs found
Ariel - Volume 9 Number 6
Executive Editor
Seth B. Paul
Associate Editor
Warren J. Ventriglia
Business Manager
Fredric Jay Matlin
University News
John Patrick Welch
World News
George Robert Coar
Editorials Editor
Steve Levine
Features
Mark Rubin
Sports Editor
Eli Saleeby
Photo Editor
Ken Buckwalter
Circulation
Victor Onufreiczuk
Lee Wugofski
Graphics and Art
Steve Hulkower
Commons Editor
Brenda Peterso
Graphene-Nanodiamond Heterostructures and their application to High Current Devices
Graphene on hydrogen terminated monolayer nanodiamond heterostructures provides a new way to improve carrier transport characteristics of the graphene, offering up to 60% improvement when compared with similar graphene on SiO2/Si substrates. These heterostructures offers excellent current-carrying abilities whilst offering the prospect of a fast, low cost and easy methodology for device applications. The use of ND monolayers is also a compatible technology for the support of large area graphene films. The nature of the C-H bonds between graphene and H-terminated NDs strongly influences the electronic character of the heterostructure, creating effective charge redistribution within the system. Field effect transistors (FETs) have been fabricated based on this novel herterostructure to demonstrate device characteristics and the potential of this approach
Structural and Functional Characterization of Malate Synthase G from Opportunistic Pathogen Pseudomonas aeruginosa
Pseudomonas aeruginosa is an opportunistic human pathogen recognized as a critical threat by the World Health Organization due to the dwindling number of effective therapies available to treat infections. Over the last decade, it has become apparent that the glyoxylate shunt plays a vital role in sustaining P. aeruginosa during infection scenarios. The glyoxylate shunt comprises two enzymes: isocitrate lyase and malate synthase isoform G. Inactivation of these enzymes has been reported to abolish the ability of P. aeruginosa to establish infection in a mammalian model system, yet we still lack the structural information to support drug design efforts. In this work, we describe the first X-ray crystal structure of P. aeruginosa malate synthase G in the apo form at 1.62 Å resolution. The enzyme is a monomer composed of four domains and is highly conserved with homologs found in other clinically-relevant microorganisms. It is also dependent on Mg2+ for catalysis. Metal ion binding led to a change in the intrinsic fluorescence of the protein, allowing us to quantitate its affinity for Mg2+. We also identified putative drug binding sites in malate synthase G using computational analysis and, because of the high resolution of the experimental data, were further able to characterize its hydration properties. Our data reveal two promising binding pockets in malate synthase G that may be exploited for drug design.This work was supported by the European Commission’s Horizon 2020 Grant 642620 to M.W. and A.P. and BBSRC Grant BB/M019411/1 to M.W
Dynamic calorimetry and XRD studies of the nematic and twist-bend nematic phase transitions in a series of dimers with increasing spacer length
A modulated and conventional DSC study of the transitions between the twist-bend nematic (Ntb), regular nematic (N) and isotropic liquid (Iso) phases was performed on a series of difluoroterphenyl-based dimers with (CH2)n spacers; n = 5, 7, 9, 11. The enthalpy of Ntb–N transition decreases steeply with increasing n, while that of the N–Iso transition increases with n; hence, the greatest effect of increasing n is a lowering N phase enthalpy. Based on past and present X-ray scattering experiments, we estimate the average molecular conformation in the Ntb phase and perform torsion energy calculations on the spacer. From this, the lowering enthalpy of the N phase is attributed to the decreasing torsional energy cost of bringing the two terphenyls from an inclined twisted conformation in the Ntb phase, to almost parallel in the N phase. With increasing n the C–C bonds of the spacers twist less away from their trans conformation, thereby reducing the overall torsion energy of the N phase. It is speculated that the nearly continuous nature of the Ntb–N transition in n = 11 dimer is associated with the divergence of the helical pitch toward infinity which is intercepted by a final jump at the very weak (0.01 J g−1) first-order transition. Small-angle X-ray scattering results suggest similar local cybotactic layering in both nematic phases, with four sublayers, i.e. tails, mesogens, spacers, mesogens
P-rex1 cooperates with PDGFRβ to drive cellular migration in 3D microenvironments
Expression of the Rac-guanine nucleotide exchange factor (RacGEF), P-Rex1 is a key determinant of progression to metastasis in a number of human cancers. In accordance with this proposed role in cancer cell invasion and metastasis, we find that ectopic expression of P-Rex1 in an immortalised human fibroblast cell line is sufficient to drive multiple migratory and invasive phenotypes. The invasive phenotype is greatly enhanced by the presence of a gradient of serum or platelet-derived growth factor, and is dependent upon the expression of functional PDGF receptor β. Consistently, the invasiveness of WM852 melanoma cells, which endogenously express P-Rex1 and PDGFRβ, is opposed by siRNA of either of these proteins. Furthermore, the current model of P-Rex1 activation is advanced through demonstration of P-Rex1 and PDGFRβ as components of the same macromolecular complex. These data suggest that P-Rex1 has an influence on physiological migratory processes, such as invasion of cancer cells, both through effects upon classical Rac1-driven motility and a novel association with RTK signalling complexes
Visual Similarity Perception of Directed Acyclic Graphs: A Study on Influencing Factors
While visual comparison of directed acyclic graphs (DAGs) is commonly
encountered in various disciplines (e.g., finance, biology), knowledge about
humans' perception of graph similarity is currently quite limited. By graph
similarity perception we mean how humans perceive commonalities and differences
in graphs and herewith come to a similarity judgment. As a step toward filling
this gap the study reported in this paper strives to identify factors which
influence the similarity perception of DAGs. In particular, we conducted a
card-sorting study employing a qualitative and quantitative analysis approach
to identify 1) groups of DAGs that are perceived as similar by the participants
and 2) the reasons behind their choice of groups. Our results suggest that
similarity is mainly influenced by the number of levels, the number of nodes on
a level, and the overall shape of the graph.Comment: Graph Drawing 2017 - arXiv Version; Keywords: Graphs, Perception,
Similarity, Comparison, Visualizatio
Computational Model for Urban Growth Using Socioeconomic Latent Parameters
Land use land cover changes (LULCC) are generally modeled using multi-scale
spatio-temporal variables. Recently, Markov Chain (MC) has been used to model
LULCC. However, the model is derived from the proportion of LULCC observed over
a given period and it does not account for temporal factors such as
macro-economic, socio-economic, etc. In this paper, we present a richer model
based on Hidden Markov Model (HMM), grounded in the common knowledge that
economic, social and LULCC processes are tightly coupled. We propose a HMM
where LULCC classes represent hidden states and temporal fac-tors represent
emissions that are conditioned on the hidden states. To our knowledge, HMM has
not been used in LULCC models in the past. We further demonstrate its
integration with other spatio-temporal models such as Logistic Regression. The
integrated model is applied on the LULCC data of Pune district in the state of
Maharashtra (India) to predict and visualize urban LULCC over the past 14
years. We observe that the HMM integrated model has improved prediction
accuracy as compared to the corresponding MC integrated modelComment: 12 page
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