A new modification of the chiron ACS assay for total prostate-specific antigen achieves equimolar response characteristics and improves the detection of prostate cancer

Nonequimolar-response assays for prostate-specific antigen (PSA) are criticized for overestimating total PSA in some men without prostate cancer (PCA), and underestimating total PSA in some men with PCA. We recently studied three nonequimolar-response PSA assays that had undergone modifications. While two of the studied assays achieved equimolar-response characteristics with improved areas under receiver operating characteristic (ROC) curves (AUC), the modification of the Chiron ACS PSA assay (ACS PSA2, Chiron) failed to achieve this. Recently, the ACS assay underwent another modification (ACS PSA, Bayer), which we investigated. Sera from 305 men (155 without and 150 with PCA, PSA greater than or equal to2 and less than or equal to30 mug/l, TandemE) were measured using both modifications of the ACS assay and equimolar-response reference methods (TandemR free and Tandem E, Hybritech). Molar response relative to the reference method and clinical performance (comparison of AUCs) between the previous and new ACS assay modifications were studied. The new modification of the ACS assay (ACS PSA, Bayer) achieved equimolar-response characteristics but reported lower values (average 10%) than the Tandem E assay. Compared to the previous modification (ACS PSA2, Chiron), a 3% improvement in AUC (p=0.01) was found. Using results of the redesigned equimolar-response assay (ACS PSA, Bayer), we calculated that 6 of 155 men without PCA in this sample set could be spared unnecessary biopsy compared with the previous nonequimolar-response assay (ACS PSA2, Chiron) without missing additional PCA (90% sensitivity). These data provide additional evidence for clinical advantages of equimolar-response over nonequimolar-response PSA assay formats

Audience synchronies in live concerts illustrate the embodiment of music experience

A study of 132 audience members of three classical public concerts (all three staged the same chamber music pieces by Ludwig van Beethoven, Brett Dean, and Johannes Brahms) had the goal of analyzing the physiological and motor responses of audiences. It was assumed that the music would induce synchronous physiology and movement in listeners (induction synchrony). In addition to hypothesizing that such synchronies would be present, we expected that they were linked to participants' aesthetic experiences, their affect and personality traits, which were assessed by questionnaires before and after the concerts. Clear evidence was found of physiological synchrony (heart rate, respiration rate, skin conductance response) as well as movement synchrony of the audiences, whereas breathing behavior was not synchronized. Thus the audiences of the three concerts resonated with the music, their music perception was embodied. There were links between the bodily synchrony and aesthetic experiences: synchrony, especially heart-rate synchrony, was higher when listeners felt moved emotionally and inspired by a piece, and were immersed in the music. Personality traits were also associated with the individual contributions to induction synchrony

Biobjective Optimization of Vibration Performance of Steel-Spring Floating Slab Tracks by Four-Pole Parameter Method Coupled with Ant Colony Optimization

Steel-spring floating slab tracks are one of the most effective methods to reduce vibrations from underground railways, which has drawn more and more attention in scientific communities. In this paper, the steel-spring floating slab track located in Track Vibration Abatement and Control Laboratory was modeled with four-pole parameter method. The influences of the fastener damping ratio, the fastener stiffness, the steel-spring damping ratio, and the steel-spring stiffness were researched for the rail displacement and the foundation acceleration. Results show that the rail displacement and the foundation acceleration will decrease with the increase of the fastener stiffness or the steel-spring damping ratio. However, the rail displacement and the foundation acceleration have the opposite variation tendency for the fastener damping ratio and the steel-spring stiffness. In order to optimize the rail displacement and the foundation acceleration affected by the fastener damping ratio and the steel-spring stiffness at the same time, a multiobjective ant colony optimization (ACO) was employed. Eventually, Pareto optimal frontier of the rail displacement and the foundation acceleration was derived. Furthermore, the desirable values of the fastener damping ratio and the steel-spring stiffness can be obtained according to the corresponding Pareto optimal solution set

Integrating GeoDesign with Landscape Sustainability Science

With an increasing world population and accelerated urbanization, the development of landscape sustainability remains a challenge for scientists, designers, and multiple stakeholders. Landscape sustainability science (LSS) studies dynamic relationships among landscape pattern, ecosystem services, and human well-being with spatially explicit methods. The design of a sustainable landscape needs both landscape sustainability–related disciplines and digital technologies that have been rapidly developing. GeoDesign is a new design method based on a new generation of information technology, especially spatial information technology, to design land systems. This paper discusses the suitability of GeoDesign for LSS to help design sustainable landscapes. Building on a review of LSS and GeoDesign, we conclude that LSS can utilize GeoDesign as a research method and the designed landscape as a research object to enrich and empower the spatially explicit methodology of LSS. To move forward, we suggest to integrate GeoDesign with LSS from six perspectives: strong/weak sustainability, multiple scales, ecosystem services, sustainability indicators, big data application, and the sense of place. Toward this end, we propose a LSS-based GeoDesign framework that links the six perspectives. We expect that this integration between GeoDesign and LSS will help advance the science and practice of sustainability and bring together many disciplines across natural, social, and design sciences

Mutations of the SLIT2–ROBO2 pathway genes SLIT2 and SRGAP1 confer risk for congenital anomalies of the kidney and urinary tract

Congenital anomalies of the kidney and urinary tract (CAKUT) account for 40–50 % of chronic kidney disease that manifests in the first two decades of life. Thus far, 31 monogenic causes of isolated CAKUT have been described, explaining ~12 % of cases. To identify additional CAKUT-causing genes, we performed whole-exome sequencing followed by a genetic burden analysis in 26 genetically unsolved families with CAKUT. We identified two heterozygous mutations in SRGAP1 in 2 unrelated families. SRGAP1 is a small GTPase-activating protein in the SLIT2–ROBO2 signaling pathway, which is essential for development of the metanephric kidney. We then examined the pathway-derived candidate gene SLIT2 for mutations in cohort of 749 individuals with CAKUT and we identified 3 unrelated individuals with heterozygous mutations. The clinical phenotypes of individuals with mutations in SLIT2 or SRGAP1 were cystic dysplastic kidneys, unilateral renal agenesis, and duplicated collecting system. We show that SRGAP1 is expressed in early mouse nephrogenic mesenchyme and that it is coexpressed with ROBO2 in SIX2-positive nephron progenitor cells of the cap mesenchyme in developing rat kidney. We demonstrate that the newly identified mutations in SRGAP1 lead to an augmented inhibition of RAC1 in cultured human embryonic kidney cells and that the SLIT2 mutations compromise the ability of the SLIT2 ligand to inhibit cell migration. Thus, we report on two novel candidate genes for causing monogenic isolated CAKUT in humans