False vacuum decay in Jordan-Brans-Dicke cosmologies

The bubble nucleation rate in a first-order phase transition taking place in a background Jordan-Brans-Dicke cosmology is examined. The leading order terms in the nucleation rate when the Jordan-Brans-Dicke field is large (i.e., late times) are computed by means of a Weyl rescaling of the fields in the theory. It is found that despite the fact that the Jordan-Brans-Dicke field (hence the effective gravitational constant) has a time dependence in the false vacuum at late times the nucleation rate is time independent

Cosmology With Many Light Scalar Fields: Stochastic Inflation and Loop Corrections

We explore the consequences of the existence of a very large number of light scalar degrees of freedom in the early universe. We distinguish between participator and spectator fields. The former have a small mass, and can contribute to the inflationary dynamics; the latter are either strictly massless or have a negligible VEV. In N-flation and generic assisted inflation scenarios, inflation is a co-operative phenomenon driven by N participator fields, none of which could drive inflation on their own. We review upper bounds on N, as a function of the inflationary Hubble scale H. We then consider stochastic and eternal inflation in models with N participator fields showing that individual fields may evolve stochastically while the whole ensemble behaves deterministically, and that a wide range of eternal inflationary scenarios are possible in this regime. We then compute one-loop quantum corrections to the inflationary power spectrum. These are largest with N spectator fields and a single participator field, and the resulting bound on N is always weaker than those obtained in other ways. We find that loop corrections to the N-flation power spectrum do not scale with N, and thus place no upper bound on the number of participator fields. This result also implies that, at least to leading order, the theory behaves like a composite single scalar field. In order to perform this calculation, we address a number of issues associated with loop calculations in the Schwinger-Keldysh "in-in" formalism.Comment: Typos corrected. Matches published versio

The periodic standing-wave approximation: post-Minkowski computation

The periodic standing wave method studies circular orbits of compact objects coupled to helically symmetric standing wave gravitational fields. From this solution an approximation is extracted for the strong field, slowly inspiralling motion of black holes and binary stars. Previous work on this model has dealt with nonlinear scalar models, and with linearized general relativity. Here we present the results of the method for the post-Minkowski (PM) approximation to general relativity, the first step beyond linearized gravity. We compute the PM approximation in two ways: first, via the standard approach of computing linearized gravitational fields and constructing from them quadratic driving sources for second-order fields, and second, by solving the second-order equations as an ``exact'' nonlinear system. The results of these computations have two distinct applications: (i) The computational infrastructure for the ``exact'' PM solution will be directly applicable to full general relativity. (ii) The results will allow us to begin supplying initial data to collaborators running general relativistic evolution codes.Comment: 19 pages, 3 figures, 1 table, RevTe

Two loop stress-energy tensor for inflationary scalar electrodynamics

We calculate the expectation value of the coincident product of two field strength tensors at two loop order in scalar electrodynamics on de Sitter background. The result agrees with the stochastic formulation which we have developed in a companion paper [2] for the nonperturbative resummation of leading logarithms of the scale factor. When combined with a previous computation of scalar bilinears [1], our current result also gives the two loop stress-energy tensor for inflationary scalar electrodynamics. This shows a secular decrease in the vacuum energy which derives from the vacuum polarization induced by the inflationary production of charged scalars.Comment: 62 pages, 1 eps figur

The organization of AMPA receptor subunits at the postsynaptic membrane: AMPARs IN CA1

AMPA receptors are the principal mediators of excitatory synaptic transmission in the mammalian central nervous system. The subunit composition of these tetrameric receptors helps to define their functional properties, and may also influence the synaptic trafficking implicated in long-term synaptic plasticity. However, the organization of AMPAR subunits within the synapse remains unclear. Here, we use postembedding immunogold electron microscopy to study the synaptic organization of AMPAR subunits in stratum radiatum of CA1 hippocampus in the adult rat. We find that GluA1 concentrates away from the center of the synapse, extending at least 25 nm beyond the synaptic specialization; in contrast, GluA3 is uniformly distributed along the synapse, and seldom extends beyond its lateral border. The fraction of extrasynaptic GluA1 is markedly higher in small than in large synapses; no such effect is seen for GluA3. These observations imply that different kinds of AMPARs are differently trafficked to and/or anchored at the synapse

When grassroots innovation movements encounter mainstream institutions: implications for models of inclusive innovation

Grassroots innovation movements (GIMs) can be regarded as initiators or advocates of alternative pathways of innovation. Sometimes these movements engage with more established science, technology and innovation (STI) institutions and development agencies in pursuit of their goals. In this paper, we argue that an important aspect to encounters between GIMs and mainstream STI institutions is the negotiation of different framings of grassroots innovation and development of policy models for inclusive innovation. These encounters can result in two different modes of engagement by GIMs; what we call insertion and mobilization. We illustrate and discuss these interrelated notions of framings and modes of engagement by drawing on three case studies of GIMs: the Social Technologies Network in Brazil, and the Honey Bee Network and People's Science Movements in India. The cases highlight that inclusion in the context of GIMs is not an unproblematic, smooth endeavour, and involves diverse interpretations and framings, which shape what and who gets included or excluded. Within the context of increasing policy interest, the analysis of encounters between GIMs and STI institutions can offer important lessons for the design of models of inclusive innovation and development

Distinctive Structural and Molecular Features of Myelinated Inhibitory Axons in Human Neocortex.

Numerous types of inhibitory neurons sculpt the performance of human neocortical circuits, with each type exhibiting a constellation of subcellular phenotypic features in support of its specialized functions. Axonal myelination has been absent among the characteristics used to distinguish inhibitory neuron types; in fact, very little is known about myelinated inhibitory axons in human neocortex. Here, using array tomography to analyze samples of neurosurgically excised human neocortex, we show that inhibitory myelinated axons originate predominantly from parvalbumin-containing interneurons. Compared to myelinated excitatory axons, they have higher neurofilament and lower microtubule content, shorter nodes of Ranvier, and more myelin basic protein (MBP) in their myelin sheath. Furthermore, these inhibitory axons have more mitochondria, likely to sustain the high energy demands of parvalbumin interneurons, as well as more 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP), a protein enriched in the myelin cytoplasmic channels that are thought to facilitate the delivery of nutrients from ensheathing oligodendrocytes. Our results demonstrate that myelinated axons of parvalbumin inhibitory interneurons exhibit distinctive features that may support the specialized functions of this neuron type in human neocortical circuits

Perineuronal Nets Suppress Plasticity of Excitatory Synapses on CA2 Pyramidal Neurons

Long-term potentiation of excitatory synapses on pyramidal neurons in the stratum radiatum rarely occurs in hippocampal area CA2. Here, we present evidence that perineuronal nets (PNNs), a specialized extracellular matrix typically localized around inhibitory neurons, also surround mouse CA2 pyramidal neurons and envelop their excitatory synapses. CA2 pyramidal neurons express mRNA transcripts for the major PNN component aggrecan, identifying these neurons as a novel source for PNNs in the hippocampus. We also found that disruption of PNNs allows synaptic potentiation of normally plasticity-resistant excitatory CA2 synapses; thus, PNNs play a role in restricting synaptic plasticity in area CA2. Finally, we found that postnatal development of PNNs on CA2 pyramidal neurons is modified by early-life enrichment, suggesting that the development of circuits containing CA2 excitatory synapses are sensitive to manipulations of the rearing environment

Polarized Secretory Trafficking Directs Cargo for Asymmetric Dendrite Growth and Morphogenesis

SummaryProper growth of dendrites is critical to the formation of neuronal circuits, but the cellular machinery that directs the addition of membrane components to generate dendritic architecture remains obscure. Here, we demonstrate that post-Golgi membrane trafficking is polarized toward longer dendrites of hippocampal pyramidal neurons in vitro and toward apical dendrites in vivo. Small Golgi outposts partition selectively into longer dendrites and are excluded from axons. In dendrites, Golgi outposts concentrate at branchpoints where they engage in post-Golgi trafficking. Within the cell body, the Golgi apparatus orients toward the longest dendrite, and this Golgi polarity precedes asymmetric dendrite growth. Manipulations that selectively block post-Golgi trafficking halt dendrite growth in developing neurons and cause a shrinkage of dendrites in mature pyramidal neurons. Further, disruption of Golgi polarity produces neurons with symmetric dendritic arbors lacking a single longest principal dendrite. These results define a novel polarized organization of neuronal secretory trafficking and demonstrate a mechanistic link between directed membrane trafficking and asymmetric dendrite growth

The organization of amyloid-β protein precursor intracellular domain-associated protein-1 in the rat forebrain

Sustained activity-dependent synaptic modifications require protein synthesis. Although proteins can be synthesized locally in dendrites, long-term changes also require nuclear signaling. Amyloid-β protein precursor intracellular domain-associated protein-1 (AIDA-1), an abundant component of the biochemical postsynaptic density fraction, contains a nuclear localization sequence, making it a plausible candidate for synapse-to-nucleus signaling. We used immunohistochemistry to study the regional, cellular, and subcellular distribution of AIDA-1. Immunostaining was prominent in the hippocampus, cerebral cortex, and neostriatum. Along with diffuse staining of neuropil, fluorescence microscopy revealed immunostaining of excitatory synapses throughout the forebrain, and immunoreactive puncta within and directly outside the nucleus. Presynaptic staining was conspicuous in hippocampal mossy fibers. Electron microscopic analysis of material processed for postembedding immunogold revealed AIDA-1 label within postsynaptic densities in both hippocampus and cortex. Together with previous work, these data suggest that AIDA-1 serves as a direct signaling link between synapses and the nucleus in adult rat brain