THE EFFECTS OF FUNGAL MEDIUM ON HATCHING RATE OF BITING MIDGE

The little black mosquitos, Forcipomyia taiwana, bite human and become one of the most annoying pests in Taiwan. Recently, the population of F. taiwana increases and invades urban and countryside. In order to effectively prevent the harassment of F. taiwana, develop control strategy is urgent and necessary. Our study found that the fungal medium influenced the length and width of the F. taiwana eggs. It also significantly decreased the hatching rate of F. taiwana. Besides, this artificial diet was more non-toxic and environment-friendly than general chemical pesticides. Thus, this study provided critical information to develop potentially useful bait of F. taiwana in the future

THE CHANGE OF KNEE KINEMATICS AFTER ANTERIOR CRUCIATE LIGAMENT DEFICIENCY AND RECONSTRUCTION DURING LANDING

The purpose of this study were to evaluate the different of knee kinematics analysis after ACLD and ACLR during landing performances. The participants were instructed to finish counter moment jump (CMJ) with arms free 5 times as hard as possible with Vicon motion system and two force platforms. The ACLD showed a significant less knee flexion degree at the peak vertical GRF compared with others. Our founding was similar to the present studies; the impulse during landing among three groups was almost the same, but the RF EMG showed lower after two ACL groups, especially in ACLD

Continuous purification of monoclonal antibody using periodic counter-current chromatography

Integrated and continuous processing of antibody drugs offers several advantages over traditional batch processing in the biotechnology industry. The flexibility of periodic counter-current (PCC) design is performed in the selection of residence time and column numbers on the capture process. In this study, we investigated the association of residence time and product recovery in the downstream PCC purification. A practical operation of PCC as a continuous capture purification step has been applied to 5L perfusion culture, 5L concentrated fed-batch culture, and 50L fed-bath culture. Using an empirical model for the protein breakthrough curve, residence time (RT) was evaluated and the loading flow rate was adjusted to achieve a target RT of 2.25 minutes for monoclonal antibody (mAb). The sample load volume for each column switching was set on 50 and 58% breakthrough curves, mAb recovery was 88 .4% and 88.9%, and buffer consumption was decreased to under half that of the batch process. Overall, more than 40 grams of purified antibody is obtained in 24 hours using a PCC purification system. Comparison of qualities of mAb analyzed by UPLC and reverse phase chromatography show that glycan profiles and purity are quite similar between antibodies obtained from PCC and batch purification, whereas the acidic variants of mAb purified by PCC is higher than that purified by batch mode. The advantages of a continuous downstream capture step are highlighted for our case study in comparison with the existing batch chromatography processes

A Comparison of Thermal Deformation of Scroll Profiles inside Oil-free Scroll Vacuum Pump and Compressor via CAE/CFD Analysis

Scroll machine is simply constructed by fixed and orbit scrolls, rotary shaft, and some mechanical components. It can impressively operate at low noise level with high reliability and high efficiency. Scroll machine achieves oil-free application through reasonable clearance control, cooling solution, and the tip seal application, and has been designed and applied as vacuum pump or compressor. In order to compactly design structure and optimize the gaps or clearances of a scroll machine, the issue of heat deformation must be considered. Deformation inside a scroll machine is not easy to be discovered, but is the necessary information for scroll profile design. In this study, the internal flow fields of oil-free scroll vacuum pump and compressor are obtained by CFD analysis. Based on the results of flow fields, this study shows the basic performance of a scroll machine, including loading on structures, gas torque, volume flow rate, and the pulsation of outlet pressure. The fluid phenomena under sub-atmospheric and positive pressure are quite different. The difference would cause different heat transfer and heat deformation. Therefore, the fluid-thermal-solid coupling analysis is also carried out. The temperature distribution of scroll structures, the thermal deformation, and gap changes are also discussed in this study

Development of an indirect method of microalgal lipid quantification using a lysochrome dye, Nile red

Earlier studies showed that the lipophilic dye, Nile red (9-diethylamino 5H-benzo[α]phenoxazine-5-one) can be used to measure the lipid content of microalgae by cellular in vivo fluorescence. It was observed that a higher amount of lipid present in lipid droplets of microalgal cells would result in higher degree of emitted fluorescent light. In this present study, the feasibility of using Nile red, a fluorescent dye specific for intracellular lipid droplets, as an indirect method of lipid quantifications was investigated. Following cellular staining of different microalgal species with nile red, the in vivo fluorescence of the whole cell was visualized by fluorescence microscopy (excitation: 450 to 590 nm and emission: 520 nm). Intensity of the relative in vivo fluorescence was measured using a fluorescence spectrophotometer at excitation and emission wavelengths of 485 and 590 nm, respectively. Lipid content was determined gravimetrically and the fluorescence of the extract was measured using the microemulsion method at emission and excitation wavelengths of 540 and 617 nm. The equivalent oil content of the extracted lipid was correlated to the fluorescence of pure olive oil using the microemulsion method. Cellular in vivo fluorescence of stained cells (ex: 485 nm and em: 590 nm), fluorescence of extracted lipid (ex: 540 nm and em: 617 nm) and gravimetrically determined lipid were linearly correlated. This suggests that Nile red can serve as a vital stain which allows a relatively rapid method of determining the lipid content of microalgal samples and is as good as the gravimetric method used for lipid determination, eliminating the requirement for the toxic solvents and timeconsuming manipulations.Keywords: Nile red, microalgae, lipid, fluorescenc

Engineering of Escherichia coli protein expression process development

It almost 30% protein drugs are expression by Escherichia coli, because of rapid growth and high production yield. We have developed E.coli base system for recombinant protein expression, scFv, Fab and vaccine. In this study we introduce example about process development for nutrient components selection. Shaker flasks were used for different nitrogen and carbon components screening by DoE. Seven media formulations for E. coli fermentation were used in this study. By changing nitrogen and carbon source ratio, product titer of target protein could be optimized, at least 1.4 folds increased. The best result from shaker flask was used in 250 mL parallel fermenter and pH, dissolved oxygen, feeding/induction strategy were evaluated. The processes from seed culture to harvest only require 64 hours. The optimized time was reduced to 32 hours. The result showed that both target protein expression and cell density value were comparable, but the total process time was significantly reduced by half Please click Additional Files below to see the full abstract