71 research outputs found

    Consistent modelling of heterogeneous lexical structures

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    International audienceThe case of lexical data as presented in a dictionary offers an interesting experimental setting for studying interoperability in the context of standardisation. It is complex enough to reflect the variability which is intrinsic to the TEI Guidelines while providing a limited observational setting for studying the granular structure of lexical entries as well as the rather high internal coherence that one specific lexical source usually has. Lexical resources also reflect the variety of analytical points of view that one may have on linguistic information ranging from quite descriptive and verbose objects in the domain of standard human-oriented dictionaries to fully structured databases like developed in the natural language processing domain

    Robust Light Driven Enzymatic Oxyfunctionalization via Immobilization of Unspecific Peroxygenase

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    Unspecific peroxygenases have attracted interest in synthetic chemistry, especially for the oxidative activation of C−H bonds, as they only require hydrogen peroxide (H2O2) instead of a cofactor. Due to their instability in even small amounts of H2O2, different strategies like enzyme immobilization or in situ H2O2 production have been developed to improve the stability of these enzymes. While most strategies have been studied separately, a combination of photocatalysis with immobilized enzymes was only recently reported. To show the advantages and limiting factors of immobilized enzyme in a photobiocatalytic reaction, a comparison is made between free and immobilized enzymes. Adjustment of critical parameters such as (i) enzyme and substrate concentration, (ii) illumination wavelength and (iii) light intensity results in significantly increased enzyme stabilities of the immobilized variant. Moreover, under optimized conditions a turnover number of 334,500 was reached

    Multi-Site N-glycan mapping study 1: Capillary electrophoresis – laser induced fluorescence

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    An international team that included 20 independent laboratories from biopharmaceutical companies, universities, analytical contract laboratories and national authorities in the United States, Europe and Asia was formed to evaluate the reproducibility of sample preparation and analysis of N-glycans using capillary electrophoresis of 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled glycans with laser induced fluorescence (CE-LIF) detection (16 sites) and ultra highperformance liquid chromatography (UHPLC, 12 sites; results to be reported in a subsequent publication). All participants used the same lot of chemicals, samples, reagents, and columns/capillaries to run their assays. Migration time, peak area and peak area percent values were determined for all peaks with >0.1% peak area. Our results demonstrated low variability and high reproducibility, both, within any given site as well across all sites, which indicates that a standard N-glycan analysis platform appropriate for general use (clone selection, process development, lot release, etc.) within the industry can be established

    Zeichenerkennung und elektronische Texterfassung

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