The Dynamic Nature of Eukaryotic Genomes

Analyses of diverse eukaryotes reveal that genomes are dynamic, sometimes dramatically so. In numerous lineages across the eukaryotic tree of life, DNA content varies within individuals throughout life cycles and among individuals within species. Discovery of examples of genome dynamism is accelerating as genome sequences are completed from diverse eukaryotes. Though much is known about genomes in animals, fungi, and plants, these lineages represent only 3 of the 60-200 lineages of eukaryotes. Here, we discuss diverse genomic strategies in exemplar eukaryotic lineages, including numerous microbial eukaryotes, to reveal dramatic variation that challenges established views of genome evolution. For example, in the life cycle of some members of the radiolaria, ploidy increases from haploid (N) to approximately 1,000N, whereas intrapopulation variability of the enteric parasite Entamoeba ranges from 4N to 40N. Variation has also been found within our own species, with substantial differences in both gene content and chromosome lengths between individuals. Data on the dynamic nature of genomes shift the perception of the genome from being fixed and characteristic of a species (typological) to plastic due to variation within and between species

Multi-core Interference-Sensitive WCET Analysis Leveraging Runtime Resource Capacity Enforcement

The performance and power efficiency of multi-core processors are attractive features for safety-critical applications, as in avionics. But increased integration and average-case performance optimizations pose challenges when deploying them for such domains. In this paper we propose a novel approach to compute a interference-sensitive Worst-Case Execution Time (isWCET) considering variable accesses delays due to the concurrent use of shared resources in multi-core processors. Thereby we tackle the problem of temporal partitioning as it is required by safety-critical applications. In particular, we introduce additional phases to state-of-the-art timing analysis techniques to analyse an applications resource usage and compute an interference delay. We further complement the offline analysis with a runtime monitoring concept to enforce resource usage guarantees. The concepts are evaluated on Freescale's P4080 multi-core processor in combination with SYSGO's commercial real-time operating system PikeOS and AbsInt's timing analysis framework aiT. We abstract real applications' behavior using a representative task set of the EEMBC Autobench benchmark suite. Our results show a reduction of up to 75% of the multi-core Worst-Case Execution Time (WCET), while implementing full transparency to the temporal and functional behavior of applications, enabling the seamless integration of legacy applications

Postoperative Radiotherapy of Prostate Cancer: Adjuvant versus Early Salvage.

Results of three randomized clinical trials (RCTs) comparing adjuvant radiotherapy (ART) and early salvage radiotherapy (eSRT) of prostate carcinoma and a subsequent meta-analysis of the individual patient data from these RCTs were recently published. The results suggest that early eSRT is as effective and potentially less toxic than ART. Therefore, eSRT should be considered the standard of care. However, due to limitations in the RCTs, ART remains a valid treatment option in patients with the combination of high-risk features such as Gleason Score (GS) 8-10, positive surgical margins (R1) and pathological T-stage 3 or 4 (pT3/4). This article provides a critical appraisal of the RCTs and the rationale for recommendations adopted in the current national guidelines regarding patients with high-risk features after radical prostatectomy (RP): ART should be offered in case of pT3/pT4 and R1 and Gleason Score 8-10; ART can be offered in case of pT3/pT4 and R0 and Gleason Score 8-10 as well as in case of multifocal R1 (including pT2) and Gleason Score 8-10. In any case, the alternative treatment option of eSRT in case of rising PSA should be discussed with the patient

Unique coding for authentication and anti-counterfeiting by controlled and random process variation in L-PBF and L-DED

Additive manufacturing technologies enable various possibilities to create and modify the material composition and structure on a local level, but are often prone to undesired defects and inhomogeneities. This contribution makes use of such flaws to generate material-inherent, hidden codes and watermarks in metals for authentication and anti-counterfeiting applications. By controlled and random process variation, unique codes that can be read and authenticated by an eddy current device were produced with the processes of laser powder bed fusion (L-PBF) and laser directed energy deposition (L-DED). Two approaches are presented: First, volumetric, porous structures with a defined shape are manufactured with L-PBF. Second, coatings are fabricated by L-DED with alternating process parameters, leading to local deviations of the magnetic permeability. This non-deterministic coding approach generates a distinctive material structure that triggers high signal amplitudes in the eddy current measurement. Counterfeiting becomes impossible due to the irreproducible melt pool dynamics. Statistical hypothesis testing proves that the system is able to prevent false acceptance or rejection of a code with a certainty of 500 million to one. A low-cost setup for a novel locking system demonstrates that a code can be sensed reliably within one second

Renormalization group flows for gauge theories in axial gauges

Gauge theories in axial gauges are studied using Exact Renormalisation Group flows. We introduce a background field in the infrared regulator, but not in the gauge fixing, in contrast to the usual background field gauge. It is shown how heat-kernel methods can be used to obtain approximate solutions to the flow and the corresponding Ward identities. Expansion schemes are discussed, which are not applicable in covariant gauges. As an application, we derive the one-loop effective action for covariantly constant field strength, and the one-loop beta-function for arbitrary regulator

Air-stable aryl derivatives of pentafluoroorthotellurate

We report on two different sets of air-stable derivatives of pentafluoroorthotellurate containing fluorinated and non-fluorinated aryl groups. The acid cis-PhTeF4OH was obtained in gram scale and further transformed to Ag[cis-PhTeF4O], which was used as a cis-PhTeF4O transfer reagent to obtain [PPh4][cis-PhTeF4O]. Furthermore, the synthesis of trans-(C6F5)2TeF3OH was achieved by a selective hydrolysis of trans-(C6F5)2TeF4 in the presence of KF and subsequent protonation by aHF. Quantum-chemical calculations show a higher acidity and robustness against fluoride abstraction for trans-(C6F5)2TeF3OH compared to cis-PhTeF4OH

Monitoring of central corneal thickness after phacoemulsification—comparison of statical and rotating Scheimpflug pachymetry, and spectral-domain OCT

AIM: To explore the possibility of deploying three contactless devices (static and rotating Scheimpflug technology, spectral domain optical coherence tomography) for measuring central corneal thickness (CCT) in preoperative and postoperative examinations of cataract patients. METHODS: Totally 72 patients who had undergone surgery without complications were selected. The CCT was measured prior to the operation, as well as on the first, 5th-7th and 28th day following the operation using the Nidek NT 530-P, Sirius®, and Topcon OCT-2000 devices. RESULTS: A significant postoperative increase and subsequent decrease in CCT was identified with all three devices. The correlations were highly significant and thus reflect a very good degree of comparability at all times with the exception of the rotating Scheimpflug camera. The postoperative results from the latter differed significantly from the other devices. The correlations were Sirius/Topcon (P=0.010) and Sirius/Nidek (P<0.0005). No statistically significant difference could be identified in the comparison between Topcon and Nidek (P=0.056). CONCLUSION: All three devices are suitable for postoperative monitoring of CCT. The measurement results are only comparable to a limited extent and not interchangeable in the course of treating a single patient. This is due to the different imaging technology used in the devices and the resulting modalities for conducting the measurements