Field Effect Transistor Nanosensor for Breast Cancer Diagnostics

Silicon nanochannel field effect transistor (FET) biosensors are one of the most promising technologies in the development of highly sensitive and label-free analyte detection for cancer diagnostics. With their exceptional electrical properties and small dimensions, silicon nanochannels are ideally suited for extraordinarily high sensitivity. In fact, the high surface-to-volume ratios of these systems make single molecule detection possible. Further, FET biosensors offer the benefits of high speed, low cost, and high yield manufacturing, without sacrificing the sensitivity typical for traditional optical methods in diagnostics. Top down manufacturing methods leverage advantages in Complementary Metal Oxide Semiconductor (CMOS) technologies, making richly multiplexed sensor arrays a reality. Here, we discuss the fabrication and use of silicon nanochannel FET devices as biosensors for breast cancer diagnosis and monitoring

Development and Survivorship of Immature Angoumois Grain Moth (Lepidoptera: Gelechiidae) on Stored Corn

Life history of immature Angoumois grain moths, Sitotroga cerealella (Olivier), was studied on dent corn (Pioneer 3320) at 10, 15, 20, 25, 30, 35, and 40°C and at 43, 53-61, 75-76, and 82-87% RH under laboratory conditions. At 10 and 40°C, none of the stages survived at any relative humidity. Temperature was the main factor affecting egg incubation period, larval-pupal development time, and egg and larval-pupal survivorship. The shortest egg development times occurred at temperatures of 30°C and higher, but they increased sharply as temperature decreased. Larval-pupal development time was shortest at 30°C. Survivorship was optimal at 20-30°C for eggs and larvae-pupae, but larval-pupal survivorship decreased sharply at 15 and 35°C. Duration of larval-pupal development did not vary with sex. Newly emerged females were twofold heavier than males, and temperature and relative humidity did not affect weight. Sex ratio of emerging adults did not differ from 1:1 at any temperature or relative humidity. The optimum conditions for development of Angoumois grain moth on corn were 30°C and 75% RH. The data will be useful for determining safe storage conditions for corn and for developing a computer model for simulating population dynamics of immature S. cerealella

Synthesis of Chiral Nonracemic Tertiary α-Thio and α-Sulfonyl Acetic Esters via SN2 Reactions of Tertiary Mesylates

Syntheses of enantioenriched sulfides and sulfones via substitution of tertiary mesylate with thiolate nucleophile were achieved with modest to excellent success

Fungal Colonists of Maize Grain Conditioned at Constant Temperatures and Humidities

Fungal colonization of shelled maize (Pioneer 3320) harvested from a field near Furman, South Carolina, in 1992 was determined after 348 and 751 days of continuous storage at each of seven temperatures (10, 15, 20, 25, 30, 35, or 40°C) and four constant relative humidities, giving equilibrium grain moisture contents ranging from 9.4% to 17.5% m.c. in 28 grain conditioning environments. Twenty fungal species infected surface sterilized seeds and were recorded from these conditioned grain treatments, including species commonly found in preharvest maize [e.g. Acremonium zeae, Aspergillus flavus, Fusarium moniliforme (syn. F. verticillioides), Penicillium pinophilum (syn. P. funiculosum), etc.]. Eupenicillium cinnamopurpureum and Monascus ruber were recorded only from conditioned grain treatments. Eurotium chevalieri colonized 50-96% of the kernels from grain conditioning treatments with the highest moisture content for each incubation temperature. Grain samples with \u3e33% E. chevalieri infection had a decreased occurrence of F. moniliforme and A. zeae, and no kernels from these samples germinated. No fungi colonized more than 50% of the kernels conditioned at 30-40°C and 9.4-14.2% m.c. The results of this study indicate that individual patterns of fungal colonization during grain conditioning were a function of the survival rates for preharvest fungal colonists and their potential replacement by E. chevalieri

Extranuclear X-ray Emission in the Edge-on Seyfert Galaxy NGC 2992

We found several extranuclear (r >~ 3") X-ray nebulae within 40" (6.3 kpc at 32.5 Mpc) of the nucleus of the Seyfert galaxy NGC 2992. The net X-ray luminosity from the extranuclear sources is ~2-3 E39 erg/s (0.3-8.0 keV). The X-ray core itself (r <~ 1") is positioned at 9:45:41.95 -14:19:34.8 (J2000) and has a remarkably simple power-law spectrum with photon index Gamma=1.86 and Nh=7E21 /cm2. The near-nuclear (3" <~ r <~ 18") Chandra spectrum is best modelled by three components: (1) a direct AGN component with Gamma fixed at 1.86, (2) cold Compton reflection of the AGN component, and (3) a 0.5 keV low-abundance (Z < 0.03 Zsolar) "thermal plasma," with ~10% of the flux of either of the first two components. The X-ray luminosity of the 3rd component (the "soft excess") is ~1.4E40 erg/s, or ~5X that of all of the detected extranuclear X-ray sources. We suggest that most (~75-80%) of the soft excess emission originates from 1" < r < 3", which is not imaged in our observation due to severe CCD pile-up. We also require the cold reflector to be positioned at least 1" (158 pc) from the nucleus, since there is no reflection component in the X-ray core spectrum. Much of the extranuclear X-ray emission is coincident with radio structures (nuclear radio bubbles and large-scale radio features), and its soft X-ray luminosity is generally consistent with luminosities expected from a starburst-driven wind (with the starburst scaled from L_FIR). However, the AGN in NGC 2992 seems equally likely to power the galactic wind in that object. Furthermore, AGN photoionization and photoexcitation processes could dominate the soft excess, especially the \~75-80% which is not imaged by our observations.Comment: 34 pages AASTEX, 9 (low-res) PS figures, ApJ, in press. For full-resolution postscript file, visit http://www.pha.jhu.edu/~colbert/n2992_chandra.ps.g

The Forest and the Perfect City

I wanted the performance to deal with memory and how we experience memories of growing up. Like the paths in a land ridden with weekly earthquakes, an individual's experiences are changing constantly; sometimes intersecting, sometimes parallel, sometimes never to meet but always in flux. Memory may alter these paths of experience to the point where what really happened and what "should have" happened are indistinguishable. I hoped The Forest and The Perfect City would be a place that reminded people of a time when the monsters in the closet and under the bed were just as real as the blanket used to hide under, a time when you could be a fireman, an Olympic athlete and a doctor - all at once. I wanted to build an odd wondrous place where friendly schizophrenic dreaming was comfortable and where the REAL WORLD still had rich veins of magic; a place where physical reality and fantasy were indiscernible, where a single identity was not necessary, and where the elemental, generational building blocks we accumulate in forming the Self(s) were spread out before us. A sacred place.&nbsp; In the performance I tried to embody these notions about identity with the three people that make up the performance: myself (25 years old), a young boy (about 8 years old - my past), and an older man about my father&rsquo;s age (between 40 and 60 - my future). The boy -&nbsp; who never left the environment - spent most of the performance playing with toy blocks. The older man, buried in a pile of leaves until he emerged from the pile midway through the performance, helped form small piles of earth before burying me in earth at the end of the performance. I saw the older man&rsquo;s emergence from the leaves and my burial as a transformation from adolescence to adulthood. Yet I also wanted it to be a &ldquo;passing of the torch&rdquo; - handing over all my responsibilities to this Body 1 would become but not really having to deal with the responsibilities because I had frozen time, frozen myself, in a space of time/place/mind where I was very content. This other Body (my future - the older man) would contend with the world where time moves and people grow older. I wanted the environment, the story, and the performance to be comforting; a place of refuge; five years old forever. &nbsp;</p

Old and Young X-ray Point Source Populations in Nearby Galaxies

We analyzed 1441 Chandra X-ray point sources in 32 nearby galaxies. The total point-source X-ray luminosity L_XP is well correlated with B, K, and FIR+UV luminosities of spiral host galaxies, and with the B and K luminosities for ellipticals. This suggests an intimate connection between L_XP and both the old and young stellar populations, for which K and FIR+UV luminosities are proxies for the galaxy mass M and star-formation rate SFR. We derive proportionality constants 1.3E29 erg/s/Msol and 0.7E39 erg/s/(Msol/yr), which can be used to estimate the old and young components from M and SFR, respectively. The cumulative X-ray luminosity functions for the point sources have quite different slopes for the spirals (gamma ~= 0.5-0.8) and ellipticals (gamma ~= 1.4), implying *the most luminous point sources dominate L_XP* for the spirals. Most of the point sources have X-ray colors that are consistent with either LMXBs or Ultraluminous X-ray sources (ULXs a.k.a. IXOs) and we rule out classical HMXBs (e.g. neutron-star X-ray pulsars) as contributing much to L_XP. Thus, for spirals, the ULXs dominate L_XP. We estimate that >~20% of all ULXs found in spirals originate from the older (pop II) stellar populations, indicating that many of the ULXs that have been found in spiral galaxies are in fact pop II ULXs, like those in elliptical galaxies. The linear dependence of L_XP on the SFR argues for either a steepening in the X-ray luminosity function of the young (pop I) X-ray source population at L_X >~10^(38.5-39) erg/s, or a decreasing efficiency for producing all types of young X-ray point sources as the galaxy SFR increases.Comment: 33 pages AASTEX, ApJ accepted. Please download full version with figures from http://www.pha.jhu.edu/~colbert/chps_accepted.p

IP3 receptor isoforms differently regulate ER-mitochondrial contacts and local calcium transfer

Contact sites of endoplasmic reticulum (ER) and mitochondria locally convey calcium signals between the IP3 receptors (IP3R) and the mitochondrial calcium uniporter, and are central to cell survival. It remains unclear whether IP3Rs also have a structural role in contact formation and whether the different IP3R isoforms have redundant functions. Using an IP3R-deficient cell model rescued with each of the three IP3R isoforms and an array of super-resolution and ultrastructural approaches we demonstrate that IP3Rs are required for maintaining ER-mitochondrial contacts. This role is independent of calcium fluxes. We also show that, while each isoform can support contacts, type 2 IP3R is the most effective in delivering calcium to the mitochondria. Thus, these studies reveal a non-canonical, structural role for the IP3Rs and direct attention towards the type 2 IP3R that was previously neglected in the context of ER-mitochondrial calcium signaling

Pupillometric Assessment of Sleepiness in Narcolepsy

Purpose: Excessive daytime sleepiness is highly prevalent in the general population, is the hallmark of narcolepsy, and is linked to significant morbidity. Clinical assessment of sleepiness remains challenging and the common objective multiple sleep latency test (MSLT) and subjective Epworth sleepiness scale (ESS) methods correlate poorly. We examined the relative utility of pupillary unrest index (PUI) as an objective measure of sleepiness in a group of unmedicated narcoleptics and healthy controls in a prospective, observational pilot study. Methods: Narcolepsy (n = 20; untreated for >2 weeks) and control (n = 56) participants were tested under the same experimental conditions; overnight polysomnography was performed on all participants, followed by a daytime testing protocol including: MSLT, PUI, sleepiness visual analog scale (VAS), ESS, and the psychomotor vigilance test (PVT). Results: The narcolepsy and control groups differed significantly on psychomotor performance and each measure of objective and subjective sleepiness, including PUI. Across the entire sample, PUI correlated significantly with objective (mean sleep latency, SL) and subjective (ESS and VAS) sleepiness, but none of the sleepiness measures correlated with performance (PVT). Among narcoleptics, VAS correlated with PVT measures. Within the control group, mean PUI was the only objective sleepiness measure that correlated with subjective sleepiness. Finally, in an ANCOVA model, SL and ESS were significantly predictive of PUI as measure of sleepiness. Conclusion: The role of PUI in quantifying and distinguishing sleepiness of narcolepsy from sleep-satiated healthy controls merits further investigation as it is a portable, brief, and objective test