Volatiles from biofumigant plants have a direct effect on carpogenic germination of sclerotia and mycelial growth of Sclerotinia sclerotiorum

Aims Sclerotia of Sclerotinia sclerotiorum survive in soil and germinate to produce apothecia which release airborne ascospores. Current control methods rely predominantly on the use of fungicides to kill ascospores. The aim of this research was to identify potential biofumigation treatments which suppress sclerotial germination, providing a potential alternative and long-term approach to disease management. Methods Microcosm and in vitro experiments were conducted using dried and milled plant material from six different biofumigant crop plants to determine effects on carpogenic germination of sclerotia and mycelial growth of S. sclerotiorum. Results All biofumigant plants significantly reduced germination of S. sclerotiorum sclerotia in the microcosm experiments, but were less effective against larger sclerotia. In vitro experiments showed a direct effect of biofumigant volatiles on both the mycelial growth of S. sclerotiorum, and carpogenic germination of sclerotia, where the most effective treatment was B. juncea ‘Vittasso’. Conclusions It was clear from this study that biofumigant crop plants have potential as part of an integrated disease management system for control of S. sclerotiorum. The microcosm experiments described here provide a straightforward and reliable screening method for evaluating different biofumigants for activity

Blurred Definitions and Imprecise Indicators: Rethinking Social Assistance for Children’s Work

This chapter argues that the design and delivery of social assistance does not take adequate account of the nuanced role of work in children’s lives and that current interventions are therefore ill-equipped to tackle children’s harmful work. This argument is developed against a background of increasing evidence that social assistance has the potential to reduce children’s engagement with work but limited understanding of its impact on children’s engagement with harmful work. The chapter reviews a set of evaluations of social assistance schemes, and shows that few studies look beyond prevalence or intensity of work. This results in a substantial knowledge gap about the extent to which, and how, social assistance may reduce harm through work. An alternative way of understanding benefits and harms of children’s work is proposed

Genome sequencing data for wild and cultivated bananas, plantains and abacá

We performed shotgun genome sequencing on a total of 19 different Musa genotypes including representatives of wild banana species Musa acuminata and M. balibisiana, allopolyploid bananas and plantains, Fe'i banana, pink banana (also known as hairy banana) and abacá (also known as hemp banana). We aligned sequence reads against a previously sequenced reference genome and assessed ploidy and, in the case of allopolyploids, the contributions of the A and B genomes; this provides important quality-assurance data about the taxonomic identities of the sequenced plant material. These data will be useful for phylogenetics, crop improvement, studies of the complex story of intergenomic recombination in AAB and ABB allotriploid bananas and plantains and can be integrated into resources such as the Banana Genome Hub

Country reviews of social assistance in crises: A compendium of rapid assessments of the nexus between social protection and humanitarian assistance in crisis settings

© 2021 Institute of Development Studies. This is an open access report available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://opendocs.ids.ac.uk/opendocs/handle/20.500.12413/16675This collection brings together brief overviews of the social assistance landscape in eight fragile and conflict-affected settings in sub-Saharan Africa and the Middle East: Iraq, Jordan, Lebanon, Mali, Niger, Nigeria, Somalia and Yemen. These overviews were prepared as part of Better Assistance in Crises (BASIC) Research, a multi-year programme (2020–24) supported by the Foreign, Commonwealth and Development Office (FCDO) of the UK government. BASIC Research aims to inform policy and programming on effective social assistance in situations of crisis, including for those who are experiencing climate-related shocks and stressors, protracted conflict and forced displacement.UKAi

The 13th Southern Hemisphere Conference on the Teaching and Learning of Undergraduate Mathematics and Statistics

Ngā mihi aroha ki ngā tangata katoa and warm greetings to you all. Welcome to Herenga Delta 2021, the Thirteenth Southern Hemisphere Conference on the Teaching and Learning of Undergraduate Mathematics and Statistics. It has been ten years since the Volcanic Delta Conference in Rotorua, and we are excited to have the Delta community return to Aotearoa New Zealand, if not in person, then by virtual means. Although the limits imposed by the pandemic mean that most of this year’s 2021 participants are unable to set foot in Tāmaki Makaurau Auckland, this has certainly not stopped interest in this event. Participants have been invited to draw on the concept of herenga, in Te Reo Māori usually a mooring place where people from afar come to share their knowledge and experiences. Although many of the participants are still some distance away, the submissions that have been sent in will continue to stimulate discussion on mathematics and statistics undergraduate education in the Delta tradition. The conference invited papers, abstracts and posters, working within the initial themes of Values and Variables. The range of submissions is diverse, and will provide participants with many opportunities to engage, discuss, and network with colleagues across the Delta community. The publications for this thirteenth Delta Conference include publications in the International Journal of Mathematical Education in Science and Technology, iJMEST, (available at https://www.tandfonline.com/journals/tmes20/collections/Herenga-Delta-2021), the Conference Proceedings, and the Programme (which has created some interesting challenges around time-zones), by the Local Organizing Committee. Papers in the iJMEST issue and the Proceedings were peer reviewed by at least two reviewers per paper. Of the ten submissions to the Proceedings, three were accepted. We are pleased to now be at the business end of the conference and hope that this event will carry on the special atmosphere of the many Deltas which have preceded this one. We hope that you will enjoy this conference, the virtual and social experiences that accompany it, and take the opportunity to contribute to further enhancing mathematics and statistics undergraduate education. Ngā manaakitanga, Phil Kane (The University of Auckland | Waipapa Taumata Rau) on behalf of the Local Organising Committ

Pathogen diversity, epidemiology and control of sclerotinia disease in vegetable crops

Sclerotinia sclerotiorum is a necrotrophic fungal pathogen with a worldwide distribution and a wide host range, including many economically important crops. The control strategies for this pathogen and related species include using fungicides, biological control agents and cultural practices such as crop rotations. However, the genetic diversity and the long term survival structures (sclerotia) of this pathogen, combined with the recent discovery of the related species S. subarctica in England and the need for growers to implement integrated disease management strategies means that new control measures need to be sought. Biofumigation, using green manures which are macerated and ploughed into the soil, may be a useful new control approach in an integrated programme. Microcosm and in vitro experiments clearly showed that volatiles released from biofumigation crops have a direct inhibitory effect on the mycelial growth and carpogenic germination of S. sclerotiorum sclerotia. The most effective biofumigation crop for inhibiting carpogenic germination varied depending on whether the volatiles released from the biofumigant crops were in direct contact with the sclerotia when the most effective crop was Raphanus sativus ‘Terranova’, or in the vapour phase when the most effective crop was B. juncea ‘Vittasso’. Carrot root inoculations showed that the number of sclerotia produced on carrot roots was significantly affected by S. sclerotiorum isolate. However, the results also showed that the weight of individual sclerotia produced by different isolates was influenced by carrot accession, but not by S. sclerotiorum isolate. Additionally, the carrot plant and detached leaf inoculations showed significant differences in the rate of lesion progression of S. sclerotiorum on different carrot accessions, indicating differences in susceptibility to the pathogen. S. subarctica microsatellite haplotypes identified in this research were shown to be shared between Scotland and Norway, and between crop plants and meadow buttercup. However, the English population did not share any microsatellite haplotypes with any other population, and analysis indicated that this S. subarctica population in England may be isolated and inbre

Re‐engaging disaffected youth through physical activity programmes

It is a cherished belief within physical education and sport communities that participation in sport/physical activity has the potential to offer young people a range of physical, psychological and social benefits. More recently in the UK, this belief has become prominent in government policies that, among other things, are seeking to re-engage disaffected young people in order to increase their life chances and minimise the impact of anti-social behaviours upon others. Yet, the link between physical activity interventions and developing pro-social behaviours is not straightforward, and there is a lack of credible research evidence to support many of the claims made for physical activity to or to inform decisions about effective intervention design. This paper reviews key literature, focusing particularly on disaffected young people and physical activity interventions in the school context, and identifies six key issues that, we would argue, warrant consideration when planning physical activity programmes to re-engage disaffected young people. In particular, it is argued that the unprecedented levels of public and private funding available for physical activity related programmes in the UK, and the high expectations placed upon them to deliver specific measurable outcomes, mean that the need for credible monitoring and evaluation is pressing

First report of Sclerotinia subarctica nom. prov. (Sclerotinia sp. 1) causing stem rot on turnip rape (Brassica rapa subsp. oleifera) in Norway

During August 2013, white-grayish lesions, typical of Sclerotinia stem rot, had developed around leaf axils on the stems of turnip rape ‘Pepita’ in a field at the NIBIO research station Apelsvoll in Oppland County, Norway. Sclerotia were collected from inside infected turnip rape stubble and from harvested seeds, surface sterilized, bisected, and placed onto potato dextrose agar (PDA). Following 1 to 2 days incubation at 20°C, fast-growing white mycelium characteristic of Sclerotinia was observed, and within 5 to 7 days, new sclerotia had started to develop. Sclerotia size and growing pattern although variable was characteristic of S. sclerotiorum. DNA extraction, PCR amplification, and sequencing of the ITS regions of the rDNA was then carried out for 20 isolates. BLASTn analysis of 475 bp amplicons showed that 15 isolates were S. sclerotiorum, while five were identified as S. subarctica (previously called Sclerotinia sp 1; Holst-Jensen et al. 1998; Winton et al. 2006, 2007), with 100% identity to a U.K. S. subarctica isolate (Clarkson et al. 2010). A representative ITS region sequence was deposited in GenBank (accession no. KX929095). The identity of the S. subarctica isolates was further confirmed by the lack of a 304-bp intron in the LSU rDNA compared with S. sclerotiorum (Holst-Jensen et al. 1998), which was visualized by PCR amplification and gel electrophoresis. Sclerotia of two S. subarctica isolates were placed on PDA and incubated for 7 days. Agar plugs of actively growing mycelium were used for the pathogenicity testing of spring oilseed rape plants (‘Mosaik’) in the greenhouse. Plants were inoculated at growth stage BBCH 57/59 (preflowering) and BBCH 64 (40% of flowers open) by attaching two PDA plugs of actively growing mycelium per main stems with small needles, using four plants per treatment. Noninoculated PDA agar plugs were attached to the control plants. The experiment was repeated three times. Symptoms typical of stem rot appeared after 1 to 2 weeks of incubation at 16 to 20°C, 100% relative humidity. Stems started to develop white lesions with fluffy mycelium around the inoculation sites. Control plants did not show the characteristic symptoms for Sclerotinia infection. After senescence of the plants, sclerotia were collected from inside the stems and cultured on PDA. White mycelium started to grow after 1 to 2 days and new sclerotia were formed within 7 days, similar to the ones used for producing the initial isolate. Brassica oil seed crops are cultivated as important break crops in the cereal-based production system in Norway and can be severely affected by Sclerotinia stem rot. The disease is observed in all regions where Brassica oil seed crops are grown, and in severe cases, a reduction in oilseed yield of 25% has been recorded in untreated control treatments of fungicide trials. Although S. subarctica has been previously reported on wild hosts (Holst-Jensen et al. 1998), this is the first report of the pathogen on a crop plant in Norway. In the United Kingdom, Clarkson et al. (2010) demonstrated pathogenicity of S. subarctica isolated from Ranunculus acris on oilseed rape. As symptoms for S. subarctica and S. sclerotiorum are indistinguishable, S. subarctica might be present undetected in many farmer fields

The 2009 BFA Graduating Class Department of Visual Arts

The bachelor of fine arts (visual arts) program is a professional program designed to educate and train students in the history, theory and practice of the visual arts. The curriculum has been devised to produce well-rounded generalists with a solid grounding in all aspects of visual arts. The curriculum illustrates the philosophy that artistic freedom and creative expression require technical skill, intellectual awareness and a personal vision, acquired through a disciplined application of effort and a critical understanding of artistic issues, past and present