Egg clutch dehydration induces early hatching in red-eyed treefrogs, Agalychnis callidryas

Terrestrial eggs have evolved repeatedly in tropical anurans exposing embryos to the new threat of dehydration. Red-eyed treefrogs, Agalychnis callidryas, lay eggs on plants over water. Maternally provided water allows shaded eggs in humid sites to develop to hatching without rainfall, but unshaded eggs and those in less humid sites can die from dehydration. Hatching responses of amphibian eggs to dry conditions are known from two lineages with independent origins of terrestrial eggs. Here, we experimentally tested for dehydration-induced early hatching in another lineage (Agalychnis callidryas, Phyllomedusidae), representing a third independent origin of terrestrial eggs. We also investigated how dehydration affected egg and clutch structure, and egg mortality. We collected clutches from a pond in Gamboa, Panama, and randomly allocated them to wet or dry treatments at age 1 day. Embryos hatched earlier from dry clutches than from wet clutches, accelerating hatching by ∼11%. Clutch thickness and egg diameter were affected by dehydration, diverging between treatments over time. Meanwhile, mortality in dry clutches was six-fold higher than in control clutches. With this study, early hatching responses to escape mortality from egg dehydration are now known from three anuran lineages with independent origins of terrestrial eggs, suggesting they may be widespread. Further studies are needed to understand how terrestrial amphibian eggs can respond to, or will be affected by, rapid changes in climate over the next decades.Fil: Salica, María José. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias. Instituto de Ecorregiones Andinas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET - Salta. San Salvador de Jujuy; ArgentinaFil: Vonesh, James R.. Virginia Commonwealth University; Estados UnidosFil: Warkentin, Karen M.. Smithsonian Tropical Research Institute; Panamá. Boston University; Estados Unido

Scaling of polymers in aligned rods

We study the behavior of self avoiding polymers in a background of vertically aligned rods that are either frozen into random positions or free to move horizontally. We find that in both cases the polymer chains are highly elongated, with vertical and horizontal size exponents that differ by a factor of 3. Though these results are different than previous predictions, our results are confirmed by detailed computer simulations.Comment: 4 pages, 4 figure

Effects of hatching timing on red-eyed treefrog tadpoles: relative vulnerability varies among predators but not with hatchling age-structure, growth varies with the presence of more vulnerable tadpoles [poster]

In Gamboa, Panama, undisturbed red-eyed treefrog embryos typically hatch at age 6 days, but they can hatch as early as 4 days if attacked by egg predators. Early hatchlings are less developed and more vulnerable to predatory shrimp and fish. Here we assess the effect of hatching timing on risk of predation by three common insects, with different foraging styles, that prey on red-eyed treefrog larvae. Further, we examine whether the consequences of hatching early vary with the presence of later-hatched tadpoles. We induced hatching at 4 and 6 days and exposed tadpoles to giant water bugs or aeshnid or libellulid dragonfly larvae over 24 hrs, quantifying tadpole behavior and mortality. We used substitutive designs with three treatments: early hatched, late hatched and mixed hatching ages. In no case did presence of the other age class alter mortality. Hatching age did not affect libelullid predation, which was low in both cases. Early-hatched tadpoles had higher mortality than late hatchlings with water bugs, which move throughout the water column. In contrast, early hatchlings had lower mortality than late hatchlings with aeshnids, which cue strongly on movement. Late-hatched tadpoles were more active than early hatchlings, and movement was reduced in the presence of aeshnids. Combining across this and previously published studies, early hatchlings are more vulnerable than late hatchlings to most (3/5) predators. Variation in relative, age-specific hatchling vulnerability to predators with different foraging styles is consistent with developmental changes in tadpole behavior

The Cost of Hatching Early: Vulnerability and Exposure to Predators [poster]

Arboreal red-eyed treefrog embryos can hatch prematurely in response to egg predators. Induced early hatchlings face aquatic predators when less developed and for longer than do later hatchlings; both factors may alter survival through the high-risk early larval period. Prior work focused on developmental effects of induced hatching. In 24 h trials, less developed hatchlings were more vulnerable to three aquatic predators (shrimp, fish, water bugs) but had similar or better survival than full-term hatchlings with libellulid and aeschnid dragonfly nymphs. However, it is unknown how developmental effects and duration of exposure to predators combine to create the net effect of early hatching. Here we measured costs of early hatching over a 72 h period, from first hatching competence past peak spontaneous hatching. Embryos hatched at age 4 or 6 days were exposed to water bugs, aeshnids, or libellulids. Early-hatched tadpoles suffered higher mortality than late hatchlings with all three predators. The longer exposure of early hatchlings to aquatic predators reversed their initial phenotypic advantage with aeshnids and revealed a cost of early hatching with libellulids where none was apparent from phenotypes alone. In other experiments, early-hatched tadpoles reared without predators grew more quickly than those reared with predators cues, which grew more quickly than age-matched embryos, revealing phenotypic effects of both predator cues and the egg vs. aquatic environment. Nonetheless, any potential benefits of predator-induced phenotypes on tadpole survival were insufficient to compensate for the increased duration of predator exposure that is a consequence of hatching early. From the Conference Program at http://www.sicb.org/meetings/2011/schedule/abstractdetails.php3?id=23

Patterns of Eating Behavior among 13-Year-Old Adolescents and Associated Factors: Findings from the Generation XXI Birth Cohort

Eating behavior adopted during adolescence may persist into adulthood. The aims of this study were to identify eating behavior patterns among Portuguese adolescents and to explore whether groups differ in terms of early life and family characteristics, severity of depressive symptoms, and body mass index (BMI) z-score. Participants were 3601 13-year-olds enrolled in the birth cohort Generation XXI. Eating behavior was assessed using the self-reported Adult Eating Behavior Questionnaire (AEBQ), validated in this sample. The severity of depressive symptoms was measured through the Beck Depression Inventory (BDI-II), and data on sociodemographic and anthropometrics were collected at birth and 13-years-old. Latent class analysis was conducted, and associations were estimated using multinomial logistic regression models. Five patterns of individuals were identified: Picky eating, Disinterest towards food, Food neophilia, Emotional eating, and Food attractiveness. The adolescents' sex, maternal education, BMI z-score, and severity of depressive symptoms were significantly associated with the identified patterns. In particular, adolescents with a higher BMI z-score were more likely in Food neophilia while individuals with more severe depressive symptoms were in the Picky eating, Emotional eating, and Food attractiveness patterns. These findings suggest a starting point for the development and planning of targeted public health interventions

Evaluating the competitive ability of semi-leafless field pea cultivars

Non-Peer ReviewedField pea (Pisum sativum L.) is an important grain legume in Western Canada. Growers can, however, be reluctant to include pulse crops in their rotation because they are poor competitors with weeds. Developing more competitive field pea cultivars is important to ameliorate weed competition. The identification of competitive cultivars and the traits conferring competitive ability should lead to the development of more competitive field pea cultivars. The objective of this research was to evaluate the ability of semi-leafless field pea cultivars to suppress and withstand weed competition and to identify traits that may confer competitive ability in field pea. Field experiments were conducted in 2012 at Floral, Saskatchewan and St. Albert, Alberta. Fourteen semi-leafless field pea cultivars with divergent pedigree, vine length, seed size, and market classes were seeded at a target density of 75 plants m-2 under weedy and weed-free conditions. Imidazolinone-tolerant wheat (c.v. CDC Imagine) and canola (c.v. 45H73) were planted as pseudo weeds at a target density of 20 plants m-2 in the weedy plots. Variables measured were leaf area index, plant height, pea biomass, weed biomass, pea yield, and weed seed production. Data were subjected to ANOVA using the mixed model procedure in SAS. There was no cultivar by treatment interaction for pea yield at Floral, so cultivars did not differ under treatments. CDC Dakota produced the greatest pea yield and Reward produced the poorest pea yield. CDC Dakota was among the best for pea biomass production at both sites, compared to CDC Leroy, which was among the worst at both sites. CDC Dakota was also among the best for the low weed seed production at Floral. CDC Mozart, CDC Patrick, and Cutlass were among the best at Floral for ability to withstand competition at Floral. While, CDC Dakota, CDC Meadow, and CDC Patrick were among the best for their ability to compete at Floral. At both sites, no correlations were strong enough to show which traits are conferring competitiveness in semi-leafless field pea cultivars

Evaluating the competitive ability of semi-leafless field pea cultivars

Non-Peer ReviewedField pea (Pisum sativum L.) is an important grain legume in Western Canada. Growers can, however, be reluctant to include pulse crops in their rotation because they are poor competitors with weeds. Developing more competitive field pea cultivars is important to mitigate weed competition. The identification of competitive cultivars and the traits conferring competitive ability should lead to the development of more competitive field pea cultivars. The objective of this research was to evaluate the ability of semi-leafless field pea cultivars to suppress and withstand weed competition and to identify traits that may confer competitive ability in field pea. Field experiments were conducted in 2012 and 2013 at Floral, Saskatchewan, Saskatoon, Saskatchewan and St. Albert, Alberta. Fourteen semi-leafless field pea cultivars were seeded at a target density of 75 plants m-2 under weedy and weed-free conditions. Imidazolinone-tolerant wheat (c.v. CDC Imagine) and canola (c.v. 45H73) were planted as pseudo weeds in the weedy plots. There was no cultivar by treatment interaction for all of the measured variables thus, cultivars did not differ in the presence or absence of weed competition. CDC Dakota produced the greatest pea yield and Reward produced the poorest pea yield at Saskatchewan. CDC Dakota and CDC Striker were among the best for pea biomass production at Saskatchewan, compared to Reward, which was among the worst. CDC Centennial and CDC Mozart were significantly better at Saskatchewan for their ability to withstand competition, while CDC Dakota, CDC Patrick, and CDC Meadow were statistically the best in their ability to compete with the pseudo weeds. At Alberta, CDC Striker and CDC Dakota were statistically best in their ability to compete with the pseudo weeds, versus Cooper and Stratus, who were among the poorest. At both Saskatchewan and Alberta, no correlations were strong enough to show which traits are conferring competitiveness in semi-leafless field pea cultivars

Body image dissatisfaction and experimental pressure pain sensitivity in a cohort of 13-year-old adolescents

Objective: We aimed to quantify the associations between body image (dis)satisfaction and pressure pain thresholds in adolescents, using data from Generation XXI, a population-based cohort study in Portugal. Methods: We assessed 1785 13-year old adolescents cross-sectionally. Body image satisfaction was measured using the Children's Figure Rating Scale. Pain detection and tolerance thresholds were assessed using cuff pressure algometry. We quantified the associations between body image categories (satisfied, prefers slightly thinner, prefers much thinner, and prefers heavier) and pain detection and tolerance thresholds using linear and logistic regression for continuous and binary (odds of achieving the highest distribution quarter) outcomes, respectively. Models were adjusted to pubertal stage and body mass index. Results: Adolescents who desired a heavier silhouette had lower pressure pain tolerance thresholds when compared to those who were satisfied (linear regression coefficient: −3.95; 95% confidence interval: −6.68, −1.21), which was more precise in boys (−3.51; −7.17, −0.08). Those adolescents also had lower odds of achieving the highest quarter of pressure pain tolerance threshold (odds ratio: 0.66; 0.48, 0.90), especially girls (0.58; 0.35, 0.98). Adolescents who desired much thinner silhouettes had lower odds of achieving the highest quarter of pressure pain tolerance (0.68; 0.46, 1.00), and this was clearer in girls (0.66; 0.48, 0.90). Pain detection thresholds did not show robust associations with body image dissatisfaction. Conclusion: Our study suggests an association between satisfaction with one's silhouette and pain tolerance in adolescents from the general population, arguing for an integrated approach to the assessment of body image and pain sensitivity. © 2022 The AuthorsThis study was funded by the European Regional Development Fund (ERDF) , through COMPETE 2020 Operational Programme ‘Competitiveness and Internationalization’ together with national funding from the Foundation for Science and Technology (FCT) - Portuguese Ministry of Science, Technology and Higher Education - through the projects “ STEPACHE - The pediatric roots of amplified pain: from contextual influences to risk stratification ” ( POCI-01-0145-FEDER-029087 , PTDC/SAU-EPI/29087/2017 ), “ Appetite regulation and obesity in childhood: a comprehensive approach towards understanding genetic and behavioural influences ” ( PTDC/SAU-EPI/30334/2017/ POCI-01-0145- FEDER-030334) , and by the Epidemiology Research Unit - Instituto de Saúde Pública, Universidade do Porto (EPIUnit) ( POCI-01-0145-FEDER-006862 ; UID/DTP/04750/2019 ; LA/P/0064/2020 ), Administração Regional de Saúde Norte (Regional Department of the Portuguese Ministry of Health) and Calouste Gulbenkian Foundation . This work was also supported by a research grant from FOREUM Foundation for Research in Rheumatology (Career Research Grant). Funding sources were not involved in the design, conduct, analysis or writing of the present results