Diethanolamide derivatives as a potential enhanced oil recovery agent from indonesian castor oil and used frying oil: isolation, synthesis, and evaluation as nonionic biosurfactants

Mono-, di- and trihydroxy fatty acids were reacted in a free-solvent amidation reaction with diethanolamine to obtain the derivative of dialkanolamides.The ricinoleic acid and 9,10,12-trihydroxystearic acid were isolated and prepared from Indonesian castor oil, while 9,10-dihydroxystearic acid was synthesized from the used frying oil. Amidation reaction of methyl ricinoleate, 9,10-dihydroxystearic acid and 9,10,12-trihydroxystearic acid gave the dialkanolamide derivatives in 93.10, 49.66, and 38.78% yield, respectively. The chemical structure of the synthesized products was elucidated by FTIR and GC-MS through silylation method. The prepared compounds were evaluated for their physicochemical properties as nonionic biosurfactant such as Hydrophilic-Lyophilic Balance value, interfacial tension, critical micelle concentration, foam stability, emulsification index, and emulsion stability. It was found that more hydroxyl groups contribute to a higher Hydrophilic-Lyophilic Balance value, foam stability, emulsification index, and lower interfacial tension

Antalogi puisi: karya anak-anak bangsa dari Papua

Antalogi puisi karya anak-anak bangsa dari Papua merupakan salah satu bentuk apresiasi Balai Bahasa Papua, Badan Pengembangan dan Pembinaan Bahasa, Kementerian Pendidikan dan Kebudayaan terhadap anak-anak bangsa yang memiliki keterampilan menulis sastra, terutama puisi. Hal ini dilakukan mengingat pemahaman masyarakat terhadap konsep estetika masih kurang. Puisi dalam buku ini merupakan hasil karya sastra siswa-siswi sekolah dasar dan sekolah menengah pertama yang mengikuti sayembara penulisan puisi bagi siwa SD dan SMP se-Papua dan Papua Barat

The Effect of Exogenous Nitrate on LCO Signalling, Cytokinin Accumulation, and Nodule Initiation in Medicago truncatula

Nitrogen fixation by rhizobia is a highly energy-demanding process. Therefore, nodule initiation in legumes is tightly regulated. Environmental nitrate is a potent inhibitor of nodulation. However, the precise mechanism by which this agent (co)regulates the inhibition of nodulation is not fully understood. Here, we demonstrate that in Medicago truncatula the lipo-chitooligosaccharide-induced accumulation of cytokinins is reduced in response to the application of exogenous nitrate. Under permissive nitrate conditions, perception of rhizobia-secreted signalling molecules leads to an increase in the level of four cytokinins (i.e., iP, iPR, tZ, and tZR). However, under high-nitrate conditions, this increase in cytokinins is reduced. The ethylene-insensitive mutant Mtein2/sickle, as well as wild-type plants grown in the presence of the ethylene biosynthesis inhibitor 2-aminoethoxyvinyl glycine (AVG), is resistant to the inhibition of nodulation by nitrate. This demonstrates that ethylene biosynthesis and perception are required to inhibit nodule organogenesis under high-nitrate conditions

Data_Sheet_1.PDF

<p>Parasponia represents five fast-growing tropical tree species in the Cannabaceae and is the only plant lineage besides legumes that can establish nitrogen-fixing nodules with rhizobium. Comparative analyses between legumes and Parasponia allows identification of conserved genetic networks controlling this symbiosis. However, such studies are hampered due to the absence of powerful reverse genetic tools for Parasponia. Here, we present a fast and efficient protocol for Agrobacterium tumefaciens-mediated transformation and CRISPR/Cas9 mutagenesis of Parasponia andersonii. Using this protocol, knockout mutants are obtained within 3 months. Due to efficient micro-propagation, bi-allelic mutants can be studied in the T₀ generation, allowing phenotypic evaluation within 6 months after transformation. We mutated four genes – PanHK4, PanEIN2, PanNSP1, and PanNSP2 – that control cytokinin, ethylene, or strigolactone hormonal networks and that in legumes commit essential symbiotic functions. Knockout mutants in Panhk4 and Panein2 displayed developmental phenotypes, namely reduced procambium activity in Panhk4 and disturbed sex differentiation in Panein2 mutants. The symbiotic phenotypes of Panhk4 and Panein2 mutant lines differ from those in legumes. In contrast, PanNSP1 and PanNSP2 are essential for nodule formation, a phenotype similar as reported for legumes. This indicates a conserved role for these GRAS-type transcriptional regulators in rhizobium symbiosis, illustrating the value of Parasponia trees as a research model for reverse genetic studies.</p