Resistance to intercompartmental mass transfer limits β2-microglobulin removal by post-dilution hemodiafiltration

Although clearance of β2-microglobulin is greater with hemodiafiltration than with high-flux hemodialysis, β2-microglobulin concentrations after long-term hemodiafiltration are only slightly less than those obtained with high-flux hemodialysis. Resistance to β2-microglobulin transfer between body compartments could explain this observation. β2-Microglobulin kinetics were determined in patients receiving on-line post-dilution hemodiafiltration for 4 h with 18 l of filtration. Plasma β2-microglobulin concentrations were measured during and for 2 h following hemodiafiltration and immediately before the next treatment. The filter clearance of β2-microglobulin was determined from arterial and venous concentrations. The β2-microglobulin generation rate was calculated from the change in the plasma concentration between treatments. The intercompartmental clearance was obtained by fitting the observed concentrations to a two-compartment, variable volume model. The plasma clearance of β2-microglobulin by the filter was 73±2 ml/min. Plasma β2-microglobulin concentrations decreased by 68±2% from pre- to post-treatment (27.1±2.2–8.5±0.7 mg/l), but rebounded by 32±3% over the next 90 min. The generation rate of β2-microglobulin was 0.136±0.008 mg/min. The model fit yielded an intercompartmental clearance of 82±7 ml/min and a volume of distribution of 10.2±0.6 l, corresponding to 14.3±0.7% of body weight. Hemodiafiltration provides a β2-microglobulin clearance of similar magnitude to the intercompartmental clearance within the body. As a result, intercompartmental mass transfer limits β2-microglobulin removal by hemodiafiltration. This finding suggests that alternative strategies, such as increased treatment times or frequency of treatment, are needed to further reduce plasma β2-microglobulin concentrations

Analyses of Value-added for Case-ready Beef, with Special Emphasis on Texas.

66 p

A baseline survey of dissolved methane in aquifers in Great Britain

Interest in dissolved methane (CH4) concentrations in aquifers in England, Scotland and Wales (‘Great Britain’ or GB) has grown concurrently with interest in the exploitation of unconventional gas sources (UGS). Experience, mainly from North America, has shown the importance of a pre-production baseline against which changes possibly due to UGS extraction can be compared. The British Geological Survey, aided by water utilities, private users and regulators, has compiled a unique dataset for CH4 in groundwaters of GB. This focuses principally on areas where UGS exploration is considered more likely, as indicated by the underlying geology. All the main water supply aquifers (Principal aquifers) were targeted, plus Secondary aquifers where locally important. The average dissolved CH4 concentration across GB in the aquifers sampled was 45 μg/l. Out of a total of 343 sites, 96% showed dissolved CH4 concentrations b100 μg/l, 80% b10 μg/l, and 43% b 1 μg/l. No site had a CH4 concentration above the US Department of the Interior suggested risk action level of 10,000 μg/l. While most sites were sampled only once, a sub set was monitored quarterly to determine the magnitude of seasonal or other variations. Generally these variations were minor, with 84% of sites showing variations within the range 0.5–37 μg/l, but some aquifers where the porosity was primarily fracture-related showed larger changes (0.5–264 μg/l). This may have been due to the nature of sampling at these sites which, unlike the others, did not have installed pumps. Since the regulatory compliance monitoring attending UGS operations will include the measurement of parameters such as dissolved CH4, it is essential that sampling methods are tested to ensure that reliable and comparable datasets can be obtained

Validity of a pictorial perceived exertion scale for effort estimation and effort production during stepping exercise in adolescent children

This is the author's PDF version of an article published in European Physical Education Review ©2002. The definitive version is available at http://epe.sagepub.com.Recent developments in the study of paediatric effort perception have continued to emphasise the importance of child-specific rating scales. The purpose of this study was to examine the validity of an illustrated 1 – 10 perceived exertion scale; the Pictorial Children’s Effort Rating Table (PCERT). 4 class groups comprising 104 children; 27 boys and 29 girls, aged 12.1±0.3 years and 26 boys, 22 girls, aged 15.3±0.2 years were selected from two schools and participated in the initial development of the PCERT. Subsequently, 48 of these children, 12 boys and 12 girls from each age group were randomly selected to participate in the PCERT validation study. Exercise trials were divided into 2 phases and took place 7 to 10 days apart. During phase 1, children completed 5 x 3-minute incremental stepping exercise bouts interspersed with 2-minute recovery periods. Heart rate (HR) and ratings of exertion were recorded during the final 15 s of each exercise bout. In phase 2 the children were asked to regulate their exercising effort during 4 x 4-minute bouts of stepping so that it matched randomly prescribed PCERT levels (3, 5, 7 and 9). Analysis of data from Phase 1 yielded significant (P<0.01) relationships between perceived and objective (HR) effort measures for girls. In addition, the main effects of exercise intensity on perceived exertion and HR were significant (P<0.01); perceived exertion increased as exercise intensity increased and this was reflected in simultaneous significant rises in HR. During phase 2, HR and estimated power output (POapprox) produced at each of the four prescribed effort levels were significantly different (P<0.01). The children in this study were able to discriminate between 4 different exercise intensities and regulate their exercise intensity according to 4 prescribed levels of perceived exertion. In seeking to contribute towards children’s recommended physical activity levels and helping them understand how to self-regulate their activity, the application of the PCERT within the context of physical education is a desirable direction for future research

A relational database of post-harvest fish losses

The article describes FISHLOSS, a database of post-harvest fish losses devised by the Natural Resources Institute (NRI), UK. The database contains 450 records of post-harvest fish losses from 150 sources. The majority of the estimates are shelf-life estimates. Designed to be a reference for people studying post-harvest fish losses, it draws attention to areas requiring future research to identify significant losses and the factors which cause them. All researchers and users are encouraged to send NRI their own estimates for inclusion in revised versions of FISHLOSS

Exploring the relation between flapping behaviour and accelerometer signal during ascending flight, and a new approach to calibration

We understand little about the energetic costs of flight in free-ranging birds; in part since current techniques for estimating flight energetics in the wild are limited. Accelerometry is known to estimate energy expenditure through body movement in terrestrial animals, once calibrated using a treadmill with chamber respirometry. The flight equivalent, a wind tunnel with mask respirometry, is particularly difficult to instigate, and has not been applied to calibrate accelerometry. We take the first steps in exploring a novel method for calibrating accelerometers with flight energy expenditure. We collected accelerometry data for Harris’s Hawks Parabuteo unicinctus flying to varying heights up to 4.1 m over a small horizontal distance; the mechanical energy expended to gain height can be estimated from physical first principles. The relationship between accelerometry and mechanical energy expenditure was strong, and while a simple wing flapping model confirmed that accelerometry is sensitive to both changes in wing beat amplitude and frequency, the relationship was explained predominately by changes in wing beat frequency, and less so by changes in amplitude. Our study provides initial, positive evidence that accelerometry can be calibrated with body power using climbing flights, potentially providing a basis for estimating flapping flight metabolic rate at least in situations of altitude gain

Yield conditions for deformation of amorphous polymer glasses

Shear yielding of glassy polymers is usually described in terms of the pressure-dependent Tresca or von Mises yield criteria. We test these criteria against molecular dynamics simulations of deformation in amorphous polymer glasses under triaxial loading conditions that are difficult to realize in experiments. Difficulties and ambiguities in extending several standard definitions of the yield point to triaxial loads are described. Two definitions, the maximum and offset octahedral stresses, are then used to evaluate the yield stress for a wide range of model parameters. In all cases, the onset of shear is consistent with the pressure-modified von Mises criterion, and the pressure coefficient is nearly independent of many parameters. Under triaxial tensile loading, the mode of failure changes to cavitation.Comment: 9 pages, 8 figures, revte

Forage quality, mineral constituents, and performance of beef yearlings grazing two crested wheatgrasses

In the central Great Plains, crested wheatgrasses (Agropyron cristatum (L.) Gaetner and A. desertorium (Fischer ex Link) Schultes] are best utilized for early spring and late fall grazing. The principal objective of this study was to determine if beef (Bos taurus L.) yearlings grazing 'Ruff' (A. cristatum) during the spring grazing season had higher average daily gains and gains per hectare than cattle grazing `Nordan' (A. desertorum). These cultivars were evaluated in grazing trials (four replications) in eastern Nebraska in 1985, 1986, and 1987. The 0.8-ha pastures were seeded in the fall of 1983 on a Typic Argiudoll soil and were fertilized annually with 68 to 90 kg N ha-1. Grazing was for 6 wk each spring by yearling steers with a beginning average weight of 250 kg. Averaged over 3 yr, Ruff produced higher gains per hectare than Nordan (272 vs 245 kg ha-1) probably because it produced more herbage because of its better persistence. At the end of the trial, the averge basal cover of Ruff and Nordan were 21 and 6%, respectively. Three-year mean average daily gains were Ruff = 1.28 vs. Nordan = 1.34 kg d-1, which were unexpected, because Ruff generally had higher forage quality as measured by an array of parameters. Ruff forage had a higher, less desirable grass tetany ratio [K/(Mg +Ca)) than Nordan (2.6 vs. 2.3) averaged over 3 yr. Cattle grazing Ruff had lower blood serum Mg levels than cattle grazing Nordan (15.4 vs. 16.2 mg L-1, both of which were below the hypomagnesemia threshold of 18 mg L-1. This condition may have reduced intake and animal gains. These results indicate the need for evaluating pasture and range grass cultivars under grazing conditions