تحضير وتوصيف وتقييم النشاط البيولوجي للمركب (E)- N’-(2,5-dimethoxybenzalidene) naphthalene-2-sulfonohydrazide

Condensation reaction of naphthalene -2-Sulfonylhydrazide, as starting material with 2,5-dimethoxy benzaldehydewas used to produce (E)-N’-(2,5-dimethoxybenzalidene) naphthalene-2-sulfonohydrazide. The Schiff base product was isolated, purified and then spectrally characterized via UV-Vis, GC/MS, FT-IR, 1H and 13C NMR analysis, where strong evidences confirmed the formation of the desired product. Antimicrobial activity of Schiff base product was evaluated in vitro against several types of bacteria such as: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and MRSA by Minimum Inhibitory Concentration (MIC) test using tetracycline (TE) as a standard antibiotic. The tests showed a promising bacteriostatic effect of this compound against gram negative bacteria such as P. aeruginosa and K. pneumoniae, such character is valuable for biological applications

Crystal structure of 4,4-dimethyl-2-(trifluoromethyl)-4,5-dihydro-1H-imidazole, C6H9F3N2

C6H9F3N2, monoclinic, P21/n (no. 14), a = 10.6224(9) Å, b = 11.8639(9) Å, c = 13.3139(11) Å, β = 105.903(3)°, V = 1613.6(2) Å3, Z = 8, Rgt(F) = 0.0618, wRref(F2) = 0.1629, T = 102(2) K [1–3]

Post-operative complications and visual outcome in eye camp patients undergoing sutureless cataract surgery at a Base Hospital in Vijayapura District, South India

Background: Cataract is the principal cause of blindness in India, accounting for 62.6%. This problem can be tackled, and the backlog of cataract blind can be reduced by performing cataract operations on a large scale. Base hospital approach with screening camps has shown good visual outcome with lesser complications.Aim and Objectives: To study the profile of postoperative complications and visual outcome in eye camp patients undergoing cataract surgery at the base hospital.Materials and Methods: In this cross‑sectional study, 413 patients were included following screening at community screening camps. Patients underwent sutureless small‑incision cataract extraction with intraocular lens implantation under local anesthesia at the base hospital. Postoperative complications and visual outcome were recorded.Results: Immediate postoperative complications were corneal edema (9.44%), striate keratitis (6.77%), expected uveitis (5.56%), secondary glaucoma (2.66%), residual lens matter (2.66%), iris prolapsed (0.48%) and shallow anterior chamber (0.24%), hyphema (0.24%) and iris incarceration (0.24%). Endophthalmitis was reported in 0.48% (one grew citrobacter, rarely reported). Late complications included posterior capsular opacification (3.87%) noted and retinal detachment (0.48%) later. Majority 83.39% of the patients had postoperative best corrected visual acuity (BCVA) between 6/6 and 6/18, 13.42% had BCVA between 6/18 and 6/60, 3.19% had BCVA < 6/60 as at 6 months follow‑up visit (overall percentage is 100%).Conclusions: The overall vision‑threatening complications after cataract extraction in the base hospital are low. Our study reflects the efficacy of the base hospital approach, in terms of visual and surgical outcome, as an important aspect from the public health point of view, which can help in clearing the cataract backlog in developing countries like India.Keywords: Cataract surgery, postoperative complication, postoperative visual outcom

ANTIMICROBIAL ACTIVITY OF CALLYSPONGIA DIFFUSA (MARINE SPONGE) ASSOCIATED ENDOPHYTIC BACTERIA L STRAINS

Objective: To screen the antimicrobial activity Of Callyspongia Diffusa (Marine Sponge) Associated Endophytic Bacterial Strains.Methods: We have isolated endophytic bacterias CDB-1 and CDB-2 from marine sponge Callyspongia diffusa and identified as Pseudomonas taiwanensis strain and Lysinibacillussphaericus strain respectively by the phylogenetic analysis. Fractions of CDB-1 and CDB-2 were screened for in vitro and in vivo antimicrobial activities against pathogenic bacteria and mycobacterium tuberculosis H37 RV strain by Minimum Inhibitory Concentration (MIC) method.Results: The lowest MIC against Kleibesella pnumoniae, Escherichia coli and Enterococcus feacalis was found to be 0.2 µg/ml and 0.4 µg/ml respectively for CDB-2. A significant antifungal activity was observed against Candida albicans (0.2-0.8 µg/ml) and Aspergillus niger (0.2-0.4 µg/ml). Further, Chloroform fraction of CDB-1 and ethyl acetate fraction of CDB-2 have shown significant anti-tubercular activity against the tested organism with MIC of 6.25µg/ml. This was supported by in vivo antimicrobial activity against K. Pneumonia infection in mice and least haemolytic activity against erythrocytes was observed. Compared to chloramphenicol.Conclusion: In this study, we have reported the marine natural species offer a rich source of bioactive metabolites that can exploit to develop novel, useful and potential therapeutic agents

تخليق وتقييم إمكانات مضادات الليباز والالتحام الجزيئي لـلمركب الجديد N \u27- (2-Hydroxy-5-nitrobenzylidene) naphthalene-2-sulfonohydrazide

N’-(2-Hydroxy-5-nitrobenzylidene) naphthalene-2-sulfonohydrazide (SB) was prepared by condensation reaction, of naphthalene-2-sulfonylchloride with 2-Hydroxy-5-nitrobenzaldehyde. The Schiff base product (SB) was isolated, purified and then spectrally characterized via UV-Vis, FT-IR, 1H and 13C NMR analysis, where strong evidences confirmed the formation of the desired product. Pancreatic porcine lipase inhibition of the Schiff base product was evaluated and compared with the reference “Orlistat”. The product was an active as a lipase enzyme inhibitor with IC50 42.65±0.97 mcg/ml. The molecular docking of the compound with porcine pancreatic lipase was investigating, the results of theoretical docking explained the experimental one since several hydrogen bonds between the Schiff base compound and amino acids in lipase were detected. Antimicrobial activity of SB product was also evaluated in vitro against several types of bacteria such as: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia and MRSA by Minimum Inhibitory Concentration (MIC) test using tetracycline (TE) as a standard antibiotic. Results showed a bacteriostatic effect of this compound against bacteria such as MRSA, P. aeruginosa and K. pneumoniae

trans-Dichlorido(2,2-dimethyl­propane-1,3-diamine)­bis­(triphenyl­phosphane)ruthenium(II)

In the title compound, [RuCl2(C5H14N2)(C18H15P)2], the RuII atom is six-coordinated, forming a slightly distorted octa­hedral geometry, with two chloride ions in an axial arrangement, and two P atoms of two triphenyl­phosphane and two chelating N atoms of the bidentate 2,2-dimethyl­propane-1,3-diamine ligand located in the equatorial plane. The average Ru—P, Ru—N and Ru—Cl bond lengths are 2.325 (18), 2.1845 (7) and 2.4123 (12) Å, respectively

Adsorption of diclofenac from aqueous solution using Cyclamen persicum tubers based activated carbon (CTAC)

AbstractThis study aims to use the tissues of Cyclamen persicum tubers to prepare activated carbon (CTAC) by different methods then to set up a thermodynamic study of the pharmaceutical diclofenac sodium (DCF) adsorption from aqueous solution onto this activated carbon. Optimum percent of DCF removal was 72% when CTAC dosage was 0.25g and DCF concentration 50mg/L. Percentage removal of DCF increases when the concentration of DCF increases as the maximum percentage removal reached 81% when DCF concentration was 70mg/L and 0.7g CTAC and pH ranging from 6 to 2.Freundlich model describes efficiently adsorption isotherm of DCF onto CTAC with n equal to 1.398 whose value indicates a favorable adsorption. This finding validates the assumption of multilayer physical adsorption process of DCF. The results showed that DCF was physically adsorbed onto CTAC, as confirmed by the values of ΔH° minor than 40kJ/mol. As ΔG° had negative charge, the adsorption process is exothermic, and the adsorption process of the DCF onto CTAC is spontaneous, depending on temperature