Research Progress on the Effect of 5-Methyltetrahydrofolic Acid on Hyperhomocysteinemia in Pregnancy

As the association of hyperhomocysteinemia in pregnancy with preterm birth, abortion, preeclampsia and other pregnancy compli-cations has attracted increasing attention, 5-methyltetrahydrofolic acid has attracted much attention as a potential treatment. In this paper, the biological mechanism of 5-methyltetrahydrofolic acid, its relationship with hyperhomocysteinemia in pregnancy and clinical studies were reviewed to review the research progress of 5-methyltetrahydrofolic acid in regulating hyperhomocysteine levels in pregnancy. The purpose of this paper is to explore the role and infuence of 5-methyltetrahydrofolic acid in the management of hyperhomocysteinemia during preg-nancy

Ideological and Political Work Should Pay Attention to the Cultivation and Enhancement of the Quality of Educators and Educatees: Based on the Study of Chinese Communist Party’s Ideological and Political Work During the Anti-Japanese War

Ideological and political work is human-oriented work and it is an interactive process of educators and educatees. During the anti-Japanese war, no matter of anti-Japanese bases or the rear areas, the Chinese Communist Party not only emphasized the enhancement of educators’ quality, but also enhanced the quality of educatees through appropriate approaches to ensure the efficiency of ideological and political work. The practice of CCP’s ideological and political work during the anti-Japanese war tells us that, we must pay same attention to the cultivation and enhancement of the quality of educators and educatees in ideological and political work and either can be neglected

Wave In in auditory brainstem response suggests a high possibility of a high jugular bulb

BackgroundWave In, which refers to the negativity between waves I and II in auditory brainstem response (ABR), is an electrophysiological phenomenon observed in previous studies. The term “high jugular bulb” (HJB) describes a jugular bulb that is located in a high position in the posterior aspect of the internal acoustic canal. The present study aimed to explore the correlation between wave In and the possibility of a HJB.MethodsThis retrospective study included a cohort of pediatric patients diagnosed with profound hearing loss who were enrolled in a government-sponsored cochlear implantation program at an academic medical center between January 2019 and December 2022. The analysis involved examining the results obtained from the ABR test and high-resolution computed tomography (HRCT) of the temporal bone in the patients. The position of the jugular bulb was classified according to the Manjila and Semaan classification.ResultsA total of 221 pediatric patients were included in the study. Twenty-four patients, with a median age of 3 years and a range of 1–7 years, showed significant bilateral (n = 21) or unilateral (n = 3) wave In (mean latency: right ear, 2.16 ms ± 0.22 ms; left ear, 2.20 ms ± 0.22 ms). The remaining 197 patients showed an absence of ABR. The HRCT images revealed that 18 of the 24 patients (75%) had HJB, but only 41 of the 197 patients who lacked ABR (20.8%) showed signs of HJB. The ratio difference was considered statistically significant based on the chi-squared test (χ2 = 32.10, p < 0.01). More than 50% of the HJBs were categorized as type 4 jugular bulbs, which are located above the inferior margin of the internal auditory canal.ConclusionABR wave In in pediatric patients with profound hearing loss suggests a high possibility of HJB. The physiological mechanism underlying this correlation needs further investigation

Case report: A novel 10.8-kb deletion identified in the β-globin gene through the long-read sequencing technology in a Chinese family with abnormal hemoglobin testing results

BackgroundThalassemia is a common inherited hemoglobin disorder caused by a deficiency of one or more globin subunits. Substitution variants and deletions in the HBB gene are the major causes of β-thalassemia, of which large fragment deletions are rare and difficult to be detected by conventional polymerase chain reaction (PCR)-based methods.Case reportIn this study, we reported a 26-year-old Han Chinese man, whose routine blood parameters were found to be abnormal. Hemoglobin testing was performed on the proband and his family members, of whom only the proband's mother had normal parameters. The comprehensive analysis of thalassemia alleles (CATSA, a long-read sequencing-based approach) was performed to identify the causative variants. We finally found a novel 10.8-kb deletion including the β-globin (HBB) gene (Chr11:5216601-5227407, GRch38/hg38) of the proband and his father and brother, which were consistent with their hemoglobin testing results. The copy number and exact breakpoints of the deletion were confirmed by multiplex ligation-dependent probe amplification (MLPA) and gap-polymerase chain reaction (Gap-PCR) as well as Sanger sequencing, respectively.ConclusionWith this novel large deletion found in the HBB gene in China, we expand the genotype spectrum of β-thalassemia and show the advantages of long-read sequencing (LRS) for comprehensive and precise detection of thalassemia variants

Dual function filtration and catalytic breakdown of organic pollutants in wastewater using ozonation with titania and alumina membranes

Water recycling via treatment from industrial and/or municipal waste sources is one of the key strategies for resolving water shortages worldwide. Polymer membranes are effective at improving the water quality essential for recycling, but depend on regular cleaning and replacement. Pure ceramic membranes can reduce the cleaning need and last significantly longer in the same applications while possessing the possibility of operating in more aggressive environments not suitable for polymers. In the current work, filtration using a tubular ceramic membrane (�-Al2O3 or TiO2) was combined with ozonation to remove organic compounds present in a secondary effluent to enhance key quality features of the water (colour and total organic carbon, TOC) for its potential reuse. ‘Bare’ commercial �-Al2O3 filters (pore size ∼0.58 �m) were tested as a microfiltration membrane and compared with the more advanced catalytically active TiO2 layer that was formed by the sol–gel method. The presence of anatase with a 4 nm pore size at the membrane surface was confirmed by X-ray diffraction (XRD) and N2 adsorption. Filtration of the effluent over a 2 h period led to a reduction in flux to 45% and 60% of the initial values for the �-alumina and TiO2 membrane, respectively. However, a brief dose (2 min) of ozone at the start of the run resulted in reductions to only 70% of the initial flux for both membranes. It is likely that the oxide’s functional property facilitated the formation of hydroxyl (OH•) or other radicals on the membrane surface from ozone decomposition which targeted the breakdown of organic foulants thus inhibiting their deposition. Interestingly, the porous structure therefore acted in a synergistic, dual function mode to physically separate the particulates while also catalytically breaking down organic matter. The system also greatly improved the efficiency of membrane filtration for the reduction of colour, A254 (organics absorption at the wavelength of 254 nm) and TOC. The best performance came from combined ozonation (2 min ozonation time with an estimated applied ozone dose of 8 mg L−1) with the TiO2 membrane, which was able to reduce colour by 88%, A254 by 75% and TOC by 43%. It is clearly evident that a synergistic effect occurs with the process combination of ozonation and ceramic membrane filtration demonstrating the practical benefit of combining ceramic membrane filtration with conventional water ozonation

In-situ secondary growth of nanocube-based Prussian-blue film as an ultrasensitive biosensor

A regular nanostructure has been widely confirmed to result ina marked improvement in material performance in biosensing applications. In the present study, a regular nanostructured Prussian blue (PB) film with two heterogeneous crystal layers was synthesized in-situ using a secondary growth method. A PB seed layer was first controlled to form uniform cube-like crystal nuclei through an ultrasonic reaction with a single reactant. Then, well-defined 100 nm PB nanocubes were further crystallized on this seed layer using a self-assembly approach. In order to accelerate the electron transfer rate during the enzyme reaction for glucose detection, the graphene was used as the main cross-linker to immobilize glucose oxidase on the PB film. The as-prepared biosensor exhibited high electrocatalysis and electron conductivity for the detection of trace glucose with a sensitivity of 141.5 µA mM-1 cm-2, as well as excellent anti-interference ability in the presence of ascorbic acid and uric acid under a low operation potential of -0.05 V

Combined TiO2 Membrane Filtration and Ozonation for Efficient Water Treatment to Enhance the Reuse of Wastewater

The application of membrane filtration processes in wastewater treatment is gaining polularity in the world today as there is increasing demand for waste reclamation. Compared to conventional treatment methods, membrane filtration is an effective method to remove particles, microorganisms and organic matter from real wastewater, consistently achieving high quality treated water. However, the most critical limitation of current membrane filtration is membrane fowling. Improvements to membrane surfaces are investigated to reduce permeation flux

Direct Purification of Digestate Using Polymeric Ultrafiltration Membranes: Influence of Materials on Filtration Behavior and Fouling Characteristics

In-depth exploration of filtration behavior and fouling characteristics of polymeric ultrafiltration (UF) membranes can provide guidance for the selection of materials and the control of membrane fouling during the purification of digestate. In this study, four types of polymeric membranes, (polyethersulfone (PES), polysulfone (PS), polyvinylidene fluoride (PVDF), and polyacrylonitrile (PAN)), were employed to filter digestate from swine manure. The results showed that the viscosity of the digestate dropped from 45.0 ± 11.3 mPa·s to 18.0 ± 9.8 mPa·s, with an increase in temperature from 30.0 °C to 45.0 °C. The four membrane fluxes all increased by more than 30%, with the cross flow velocity increasing from 1.0 m s−1 to 2.0 m s−1. During the batch experiments, the flux maintenance abilities of the membranes were in the order: PAN > PS > PVDF > PES. There were no significant differences in the effects of membrane materials on the removal of COD, TN, and TP (p 254 removal efficiency, PS showed the highest efficiency (68.6%), while PVDF showed the lowest efficiency (63.4%). The major fouling type was irreversible hydraulic fouling, and the main elements of scaling were C, O, S, and Ca. Pseudomonadales were the dominant bacteria in the PS (26.2%) and in the PVDF (51.4%) fouling layers, while Bacteroidales were the dominant bacteria in the PES (26.8%) and in the PAN (14.7%) fouling layers. The flux recovery rates (FRRs) of the cleaning methods can be arranged as follows: NaClO > NaOH > Citric acid ≈ Tap water. After NaClO cleaning, the PVDF membrance showed the highest FRR (73.1%), and the PAN membrane showed the lowest FRR (30.1%)

Anxiety and depression levels of the general population during the rapid progressing stage in the coronavirus disease 2019 outbreak: a cross-sectional online investigation in China

Objective The outbreak of COVID-19 has major impacts on the psychological health of the public. This study aimed to investigate the anxiety and depression levels of the general population during the rapid progressing stage of COVID-19 pandemic in China and to explore the associated factors.Design and setting A cross-sectional online survey.Participants 2651 Chinese people.Measures The Hospital Anxiety and Depression Scale was used to measure their psychological health. A structured questionnaire collected possible associated factors, including sociodemographic characteristics, health information, contact history-related information, experience and perceptions, knowledge and education and adopted precautions. Multiple linear regression was conducted to explore the factors associated with anxiety and depression.Results The mean score of anxiety and depression was 4.35 and 4.38, respectively. The rates of people with anxiety and depressive symptoms (with >7 score in the subscale) were 14.15% and 17.35%, respectively. Participants without political party membership, with contact history of COVID-19, going out or gathering, taking Chinese medicine herbs, being unsatisfied with current precautions, perceiving higher risks of infection, lower knowledge and poorer health presented higher anxiety and depression levels. Moreover, those who were females, married, lived alone and wore mask were more anxious; whereas people who were younger, experienced public health crisis, did not take precautions (regular work-rest, exercise) had higher depression level.Conclusions During the rapid progressing stage of COVID-19 pandemic in China, one-seventh and one-sixth respondents presented anxiety and depression symptoms, respectively. The risk factors for anxiety and depression included the following: without political party membership, with contact history of COVID-19, going out or gathering, wearing masks, taking Chinese medicine herbs, being unsatisfied with current precautions, perceiving higher susceptibility, lower knowledge and poorer health status. Extensive information and psychological support should be provided to improve the mental health of the general population