Regulation of the formation and water permeability of endosomes from toad bladder granular cells.

Osmotic water permeability (Pf) in toad bladder is regulated by the vasopressin (VP)-dependent movement of vesicles containing water channels between the cytoplasm and apical membrane of granular cells. Apical endosomes formed in the presence of serosal VP have the highest Pf of any biological or artificial membrane (Shi and Verkman. 1989. J. Gen. Physiol. 94:1101-1115). We examine here: (a) the influence of protein kinase A and C effectors on transepithelial Pf (Pfte) in intact bladders and on the number and Pf of labeled endosomes, and (b) whether endosome Pf can be modified physically or biochemically. In paired hemibladder studies, Pfte induced by maximal serosal VP (50 mU/ml, 0.03 cm/s) was not different than that induced by 8-Br-cAMP (1 mM), forskolin (50 microM), VP + 8-Br-cAMP, or VP + forskolin. Pf was measured in endosomes labeled in intact bladders with carboxyfluorescein by a stopped-flow, fluorescence-quenching assay using an isolated microsomal suspension; the number and Pf (0.08-0.11 cm/s, 18 degrees C) of labeled endosomes was not different in bladders treated with VP, forskolin, and 8-Br-cAMP. Protein kinase C activation by 1 microM mucosal phorbol myristate acetate (PMA) induced submaximal bladder Pfte (0.015 cm/s) and endosome Pf (0.022 cm/s) in the absence of VP, but had little effect on maximal Pfte and endosome Pf induced by VP. However, PMA increased by threefold the number of apical endosomes with high Pf formed in response to serosal VP. Pf of endosomes containing the VP-sensitive water channel decreased fourfold by increasing membrane fluidity with hexanol or chloroform (0-75 mM); Pf of phosphatidylcholine liposomes (0.002 cm/s) increased 2.5-fold under the same conditions. Endosome Pf was mildly pH dependent, strongly inhibited by HgCl2, but not significantly altered by GTP gamma S, Ca, ATP + protein kinase A, and phosphatase action. We conclude that: (a) water channels cycled in endocytic vesicles are functional and not subject to physiological regulation, (b) VP and forskolin do not have cAMP-independent cellular actions, (c) activation of protein kinase C stimulates trafficking of water channels, but does not increase the number of apical membrane water channels induced by maximal VP, and (d) water channel function is sensitive to membrane fluidity. By using VP and PMA together, large quantities of endosomes containing the VP-sensitive water channel are labeled with fluid-phase endocytic markers

Novel Z2 Topological Metals and Semimetals

© 2016 American Physical Society.We report two theoretical discoveries for Z2 topological metals and semimetals. It is shown first that any dimensional Z2 Fermi surface is topologically equivalent to a Fermi point. Then the famous conventional no-go theorem, which was merely proven before for Z Fermi points in a periodic system without any discrete symmetry, is generalized so that the total topological charge is zero for all cases. Most remarkably, we find and prove an unconventional strong no-go theorem: all Z2 Fermi points have the same topological charge νZ2=1 or 0 for periodic systems. Moreover, we also establish all six topological types of Z2 models for realistic physical dimensions.postprin

Modelling of sediment nutrient fluxes for a pulsed organic load

A sediment model has been developed to simulate the Sediment Oxygen Demand (SOD) and sediment nutrient fluxes after a sudden input of Particulate Organic Matter (POM). Compared with previous sediment models, the main advances are the use of a continuous function to represent the kinetics of POM diagenesis and inclusion of the biomass content in POM diagenesis. Laboratory experiments were also carried out to investigate the SOD dynamics and sediment nutrient fluxes after an input of fish food pellets into the sediment. The simulation results of SOD and nutrient fluxes compared fairly well with the experimental measurements. Copyright © 2011 Inderscience Enterprises Ltd.postprin

Organizing the innovation process : complementarities in innovation networking

This paper contributes to the developing literature on complementarities in organizational design. We test for the existence of complementarities in the use of external networking between stages of the innovation process in a sample of UK and German manufacturing plants. Our evidence suggests some differences between the UK and Germany in terms of the optimal combination of innovation activities in which to implement external networking. Broadly, there is more evidence of complementarities in the case of Germany, with the exception of the product engineering stage. By contrast, the UK exhibits generally strong evidence of substitutability in external networking in different stages, except between the identification of new products and product design and development stages. These findings suggest that previous studies indicating strong complementarity between internal and external knowledge sources have provided only part of the picture of the strategic dilemmas facing firms

Withaferin A Suppresses Liver Tumor Growth in a Nude Mouse Model by Downregulation of Cell Signaling Pathway Leading to Invasion and Angiogenesis

Purpose: To investigate the effect of withaferin A on tumor growth and metastasis in liver in a nude mouse model.Methods: Withaferin A was injected through a portal vein to the orthotopic liver tumor in a nude mice model. Xenogen in vivo imaging system was used to monitor tumor growth and metastasis. The effect of withaferin A on tumor volume, invasive growth pattern, expression of Pyk2, upregulation of BAX/P53, apoptotic signaling and ROCK/IP10/VEGF pathway along with cytoskeletal protein actin projection formation was studied. Tumor/non-tumor margin was examined under electron microscopy. In addition, the direct effect of withaferin A on liver cancer cells and endothelial cells was further investigated.Results: A significant inhibition of tumor growth and lower incidence of lung metastasis was observed after withaferin A treatment. Withaferin A treatment led to a decrease in the incidence of intrahepatic metastasis from 90 (9 of 10) to 10 % (1 of 10, p = 0.041). There was decrease in macrophage infiltration in the liver tumors and vessels. Western blot analysis revealed inhibition of expression of Pyk2, ROCK1 protein and VEGF. Electron microscopy showed tumor vascular endothelial cell damage and significant necrosis of tumor tissues. It also suppressed formation of cytoskeletal protein actin projection involved in cell migration.Conclusion: Withaferin A inhibits liver tumor invasion and angiogenesis by downregulation of cell signalling pathway leading to invasion and angiogenesis. Therefore, withaferin A is a promising candidate for the treatment of liver tumor invasion and angiogenesis.Keywords: Withaferin A, Macrophage, Lung metastasis, Angiogenesis, Vascular endothelial growth factor, Rho kinase, Withania somnifer

Spin pump in the presence of a superconducting lead

We present a theoretical analysis of a spin pump in the presence of a superconducting lead. The spin pump is facilitated by a rotating magnetic field which provides a spin flip mechanism and hence can generate a spin current without an accompanying charge current. Using a nonequilibrium Green's function method, we obtain a general solution of the pumped charge current and spin current in both the adiabatic and non-adiabatic regimes. The numerical results for the charge current and spin current are presented as we vary different system parameters such as the gate voltage, the external magnetic field, and the pumping frequency. We find that for a quantum dot with a single resonant level in line with the Fermi energy of the left normal lead, a pure spin current is generated by a rotating magnetic field at any frequency. We have identified two kinds of photon-assisted processes which dominate at low pumping frequencies and high pumping frequencies, respectively.published_or_final_versio

Oscillatory thermopower of carbon chains: First-principles calculations

We investigate the thermoelectric transport through carbon chains connected by two Al leads. Using a Landauer-Buttiker-like formula, we calculate the thermopower and thermoconductance of Al-Cn-Al from first principles. We find that the charge transfer plays an important role in the thermoelectric transport. Because of the charge transfer, the thermopower changes sign for even-odd number of carbon atoms. The thermopower and electric conductance as a function of the gate voltage also exhibit oscillatory behaviors with a phase difference of pi/2.published_or_final_versio

Numerical Investigation of Transonic Flow-Induced Spontaneous Condensation in Micro-Ejector Nozzles

Micro-cooling systems are compact refrigeration systems widely applicable in microchemical analysis, biomedicine, and microelectromechanical systems (MEMS). These systems rely on the use of micro-ejectors to achieve precise, fast, and reliable flow and temperature control. However, the efficiency of micro-cooling systems is hindered by spontaneous condensation occurring downstream of the nozzle throat and within the nozzle itself, impacting the performance of the micro-ejector. A micro-scale ejector mathematical model describing wet steam flow was simulated to investigate the steam condensation phenomenon and its influence on flow, incorporating equations for liquid phase mass fraction and droplet number density transfer. The simulation results of wet vapor flow and ideal gas flow were compared and analyzed. The findings revealed that the pressure at the micro-nozzle outlet exceeded predictions based on the ideal gas assumption, while the velocity fell below it. These discrepancies indicated that condensation of the working fluid reduces the pumping capacity and the efficiency of the micro-cooling system. Furthermore, simulations explored the impact of inlet pressure and temperature conditions on spontaneous condensation within the nozzle. The results demonstrated that the properties of the working fluid directly influence transonic flow condensation, underscoring the importance of selecting appropriate working fluid parameters for nozzle design to ensure nozzle stability and optimal micro-ejector operation

Exploring the "energy-saving personality traits" in the office and household situation: An empirical study

Behavior-driven energy conservation has been a promising strategy for reducing building energy consumption as well as carbon emissions. With the intention of revealing the impacts of an individual’s personality basis on energy conservation behavioral attitudes and intentions in households and offices, the present study proposes and conducts an experiment in Xi’an, China with two groups for the investigation of such attitudes towards household energy-saving behavior (HESB) and office energy-saving behavior (OESB), respectively. The research adopts structural equation modeling for experiment data analysis. The analysis results suggest that the two personality traits, Agreeableness and Neuroticism, are significantly related to both HESB and OESB attitudes. Especially, agreeable people tend to present stronger energy-saving attitudes, while individuals with higher Neuroticism are less likely to do so. The results indicate that the impacts of these two traits on energy-saving attitude are found to be less influenced by different environment settings. Further, the results find that Extraversion positively influences energy-saving attitude in the office environment, while Openness only significantly works in the household environment. It is hoped that the findings of the present study can provide informative references to energy-saving intervention design as well as further studies on the spillover of pro-environmental behaviors.</jats:p