The anti-sepsis activity of the components of Huanglian Jiedu Decoction with high lipid A-binding affinity

Huanglian Jiedu Decoction (HJD), one of the classic recipes for relieving toxicity and fever, is a common method for treating sepsis in China. However, the effective components of HJD have not yet been identified. This experiment was carried out to elucidate the effective components of HJD against sepsis. Thus, seven fractions from HJD were tested using a biosensor to test their affinity for lipid A. The components obtained that had high lipid A-binding fractions were further separated, and their affinities to lipid A were assessed with the aid of a biosensor. The levels of LPS in the blood were measured, and pathology experiments were conducted. The LPS levels and mRNA expression analysis of TNF-α and IL-6 of the cell supernatant and animal tissue were evaluated to investigate the molecular mechanisms. Palmatine showed the highest affinity to lipid A and was evaluated by in vitro and in vivo experiments. The results of the in vitro and in vivo experiments indicated that the levels of LPS, TNF-α and IL-6 of the palmatine group were significantly lower than those of the sepsis model group (p \u3c 0.01). The group treated with palmatine showed strong neutralizing LPS activity in vivo. The palmatine group exhibited stronger protective activity on vital organs compared to the LPS-induced animal model. This verifies that HJD is a viable treatment option for sepsis given that there are multiple components in HJD that neutralize LPS, decrease the release of IL-6 and TNF-α induced by LPS, and protect vital organs

Review of Aircraft Vibration Environment Prediction Methods

AbstractAircraft vibration response environment prediction, which is adopted in aircraft initial vehicle development, has not been got enough attention and wide application yet. This paper briefly reviews theoretical and engineering significance of aircraft vibration response environment prediction firstly. Then the paper summarizes the main aircraft vibration response environment prediction methods and indicates their advantages, disadvantages and applicability scopes, including extrapolation of similar structure, theory analysis and analytical solution of differential dynamical equation, statistical parameter modeling, simulation calculation modeling and machine learning. Finally, the paper points out that uncertainty and non-linear structures, nonstationary signal analysis and complex vibration environment response prediction are major problems for aircraft vibration response prediction and directions for future research work

Circumferential defect detection using ultrasonic guided waves: An efficient quantitative technique for pipeline inspection

Purpose: Quantitatively detecting surface defects in a circular annulus with high levels of accuracy and efficiency has been paid more attention by researchers. The purpose of this study is to investigate the theoretical dispersion equations for circumferential guided waves and then develop an efficient technique for accurate reconstruction of defects in pipes. Design/methodology/approach: The methodology applied to determine defects in pipelines includes four steps. First, the theoretical work is carried out by developing the appropriate dispersion equations for circumferential guided waves in a pipe. In this phase, formulations of strain-displacement relations are derived in a general equidistant surface coordinate. Following that, a semi-analytical finite element method (SAFEM) is applied to solve the dispersion equations. Then, the scattered fields in a circular annulus are calculated using the developed hybrid finite element method and simulation results are in accord with the law of conservation of energy. Finally, the quantitative detection of Fourier transform (QDFT) approach is further enhanced to efficiently reconstruct the defects in the circular annuli, which have been widely used for engineering applications. Findings: Results obtained from four numerical examples of flaw detection problems demonstrate the correctness of the developed QDFT approach in terms of accuracy and efficiency. Reconstruction of circumferential surface defects using the extended QDFT method can be performed without involving the analytical formulations. Therefore, the streamlined process of inspecting surface defects is well established and this leads to the reduced time in practical engineering tests. Originality/value: In this paper, the general dispersion equations for circumferential ultrasonic guided waves have been derived using an equidistant surface coordinate and solved by the SAFEM technique to discover the relationship between wavenumber of a wave and its frequency. To reconstruct defects with high levels of accuracy and efficiency, the QDFT approach has been further enhanced to inspect defects in the annular structure

A novel approach to surface defect detection

Defects or flaws in highly loaded structures have a significant impact on the structural integrity. Early inspection of faults can reduce the likelihood of occurrence of potential disasters and limit the damaging effects of destructions. According to our previous work, a novel approach called as Quantitative Detection of Fourier Transform (QDFT) using guided ultrasonic waves is developed in this paper for efficiently detecting defects in pipeline structures. Details of this fast method consist of three steps: First, an in-house finite element code has been developed to calculate reflection coefficients of guided waves travelling in the pipe. Then, based on boundary integral equations and Fourier transform of space-wavenumber domain, theoretical formulations of the quantitative detection are derived as a function of wavenumber using Born approximation. This lays a solid foundation for QDFT method, in which a reference model in a problem with a known defect is utilized to effectively evaluate the unknown defects. Finally, the location and shape of the unknown defect are reconstructed using signal processing for noise removal. Several examples are presented to demonstrate the correctness and efficiency of the proposed methodology. It is concluded that the general two-dimensional surface defects can be detected with high level of accuracy by this fast approach

Soluble olfactory proteins in insects

Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are regarded as carriers of pheromones and odorants in insect chemoreception. These proteins are typically located in antennae, mouth organs and other chemosensory structures; however, members of both classes of proteins have been detected recently in other parts of the body and various functions have been proposed. The best studied of these non-sensory tasks is performed in pheromone glands, where OBPs and CSPs solubilise hydrophobic semiochemicals and assist their controlled release into the environment. In some cases the same proteins are expressed in antennae and pheromone glands, thus performing a dual role in receiving and broadcasting the same chemical message. Several reports have described OBPs and CSPs in reproductive organs. Some of these proteins are male specific and are transferred to females during mating. They likely carry semiochemicals with different proposed roles, from inhibiting other males from approaching mated females, to marking fertilized eggs, but further experimental evidence is still needed. Before being discovered in insects, the presence of binding proteins in pheromone glands and reproductive organs was widely reported in mammals, where vertebrate OBPs, structurally different from OBPs of insects and belonging to the lipocalin superfamily, are abundant in rodent urine, pig saliva and vaginal discharge of the hamster, as well as in the seminal fluid of rabbits. In at least four cases CSPs have been reported to promote development and regeneration: in embryo maturation in the honeybee, limb regeneration in the cockroach, ecdysis in larvae of fire ants and in promoting phase shift in locusts. Both OBPs and CSPs are also important in nutrition as solubilisers of lipids and other essential components of the diet. Particularly interesting is the affinity for carotenoids of CSPs abundantly secreted in the proboscis of moths and butterflies and the occurrence of the same (or very similar CSPs) in the eyes of the same insects. A role as a carrier of visual pigments for these proteins in insects parallels that of retinol-binding protein in vertebrates, a lipocalin structurally related to OBPs of vertebrates. Other functions of OBPs and CSPs include anti-inflammatory action in haematophagous insects, resistance to insecticides and eggshell formation. Such multiplicity of roles and the high success of both classes of proteins in being adapted to different situations is likely related to their stable scaffolding determining excellent stability to temperature, proteolysis and denaturing agents. The wide versatility of both OBPs and CSPs in nature has suggested several different uses for these proteins in biotechnological applications, from biosensors for odours to scavengers for pollutants and controlled releasers of chemicals in the environment

Draft Genome Sequence of \u3cem\u3eCercospora sojina\u3c/em\u3e Isolate S9, a Fungus Causing Frogeye Leaf Spot (FLS) Disease of Soybean

Fungi are the causal agents of many of the world\u27s most serious plant diseases causing disastrous consequences for large-scale agricultural production. Pathogenicity genomic basis is complex in fungi as multicellular eukaryotic pathogens. The fungus Cercospora sojina is a plant pathogen that threatens global soybean supplies. Here, we report the genome sequence of C. sojina strain S9 and detect genome features and predicted genomic elements. The genome sequence of C. sojina is a valuable resource with potential in studying the fungal pathogenicity and soybean host resistance to frogeye leaf spot (FLS), which is caused by C. sojina. The C. sojina genome sequence has been deposited and available at DDBJ/EMBL/GenBank under the project accession number AHPQ00000000