Effects of post-treatment electroacupuncture on ventricular monophasic action potential and cardiac function in a rat model of ischemia/reperfusion injury

Background: To determine the effects of post-treatment electroacupuncture (EA) on the electrophysiological properties of ventricular muscle in rats with ischemia/reperfusion (IR) injury. Methods: Male Sprague–Dawley (SD) rats were randomly assigned into sham-operated (SH), IR and IR + EA groups (n = 8 each). The IR model was generated by ligation of the left anterior descending (LAD) coronary artery for 30 min. After establishing the IR model, EA was administered at PC6 for 30 min while opening the coronary artery and allowing reperfusion for 30 min. Heart rate (HR), mean arterial pressure and monophasic action potential (MAP)of cardiac muscle in the outer membrane of the antetheca of the left ventricle before coronary artery ligation (T0),after coronary artery ligation for 30 min (T1) and after reperfusion for 30 min (T2) were recorded. At the same time, ventricular electrophysiological parameters including ventricular effective refractory period (ERP), conduction velocity (CV) and ventricular fibrillation threshold (VFT) were measured. Then, the cardiac function and the levels of creatine kinase-muscle/brain (CK-MB) and cardiac troponin I (cTnI) were monitored. Based on these data,monophasic action potential amplitude (MAPA), the maximum depolarization velocity (Vmax) and the MAP durations at 50% and 90% repolarization (MAPD50 and MAPD90) were calculated to determine the incidence of arrhythmia during reperfusion. Results: Compared with the SH group, the IR group showed an obviously decreased HR as well as reduced mean arterial pressure, Vmax, CV, ERP and MAPA. All indices of cardiac function except left ventricular end-diastolic pressure (LVEDP) decreased (i.e. ventricular systolic pressure (LVSP), left ventricular ejection fraction (LVEF), fractional shortening (FS) and rate of the ventricular pressure rise/drop (±dp/dt)). Furthermore, the MAPD50 and MAPD90 were prolonged, and the levels of CK-MB and cTnI increased (p 0.05). Conclusion: EA post-treatment can relieve prolongation of repolarization and slowed depolarization of ventricular muscle during IR, thus decreasing the rate of incidence of reperfusion arrhythmia

Study on elastohydrodynamic lubrication performance of double-layer composite water-lubricated bearings

Double-layer composite water-lubricated bearing is a new type of water-lubricated bearing which can integrate the good damping performance of low elastic under-layer bush and good tribological performance of plastic layer bush. This paper analyzes its elastohydrodynamic lubrication performance by fluid–structure interaction (FSI) method, and studies the effects of eccentricity ratio, rotational speed, elastic modulus distribution and thickness distribution of bearing bush on its lubrication performance. Results show that the lubrication performance of double-layer bearing is more like that of plastic bearing. As rotational speed and eccentricity ratio increase, the maximum water film pressure, the load carrying capacity and the maximum bush deformation increase significantly. As the elastic modulus of the low elastic under-layer bush decreases, the total bush deformation increases significantly, but the load carrying capacity decreases slightly. The bush thickness distribution influences the deformation distribution of both low elastic under-layer bush and plastic layer bush, but have little impact on the total bush deformation and bearing lubrication performance

Raw rehmannia radix polysaccharide can effectively release peroxidative injury induced by duck hepatitis A virus

Background: Duck viral hepatitis (DVH), caused by duck hepatitis A virus (DHAV), is a fatal contagious infectious disease which spreads rapidly with high morbidity and high mortality, and there is no effective clinical drug against DVH.Materials and Methods: Raw Rehmannia Radix Polysaccharide (RRRP), Lycii Fructus polysaccharides and Astragalus Radix polysaccharides were experimented in vitro and in vivo. Mortality rate, livers change, liver lesion scoring, peroxidative injury evaluation indexes in vitro and in vivo, and hepatic injury evaluation indexes of optimal one were detected and observed in this experiment.Results: RRRP could reduce mortality with the protection rate about 20.0% compared with that of the viral control (VC) group, finding that RRRP was the most effective against DHAV. The average liver scoring of the VC, blank control (BC), RRRP groups were 3.5, 0, 2.1. Significant difference (P<0.05) appeared between any two groups, demonstrating that it can alleviate liver pathological change. RRRP could make the hepatic injury evaluation indexes similar to BC group while the levels of the VC group were higher than other two groups in general. The levels of SOD, GSH-Px, CAT of RRRP group showed significant higher than that of VC group while the levels of NOS and MDA showed the opposite tendency, thus, RRRP could release peroxidative injury.Conclusion: RRRP was the most effective against duck hepatitis A virus (DHAV). RRRP could reduce mortality, alleviate liver pathological change, down-regulate liver lesion score, release peroxidative injury and hepatic injury. The antiviral and peroxidative injury releasing activity of RRRP for DHAV provided a platform to test novel drug strategies for hepatitis A virus in human beings.Keywords: Raw Rehmannia Radix Polysaccharide; duck hepatitis A virus; peroxidative injury; hepatic injur

Cupid: Automatic Fuzzer Selection for Collaborative Fuzzing

Combining the strengths of individual fuzzing methods is an appealing idea to find software faults more efficiently, especially when the computing budget is limited. In prior work, EnFuzz introduced the idea of ensemble fuzzing and devised three heuristics to classify properties of fuzzers in terms of diversity. Based on these heuristics, the authors manually picked a combination of different fuzzers that collaborate. In this paper, we generalize this idea by collecting and applying empirical data from single, isolated fuzzer runs to automatically identify a set of fuzzers that complement each other when executed collaboratively. To this end, we present Cupid, a collaborative fuzzing framework allowing automated, data-driven selection of multiple complementary fuzzers for parallelized and distributed fuzzing. We evaluate the automatically selected target-independent combination of fuzzers by Cupid on Google's fuzzer-test-suite, a collection of real-world binaries, as well as on the synthetic Lava-M dataset. We find that Cupid outperforms two expert-guided, target-specific and hand-picked combinations on Google's fuzzer-test-suite in terms of branch coverage, and improves bug finding on Lava-M by 10%. Most importantly, we improve the latency for obtaining 95% and 99% of the coverage by 90% and 64%, respectively. Furthermore, Cupid reduces the amount of CPU hours needed to find a high-performing combination of fuzzers by multiple orders of magnitude compared to an exhaustive evaluation

Biomaterials delivery strategies to repair degenerated intervertebral discs by regulating the inflammatory microenvironment

Intervertebral disc degeneration (IVDD) is one of the leading causes of lower back pain. Although IVDD cannot directly cause death, it can cause pain, psychological burdens, and economic burdens to patients. Current conservative treatments for IVDD can relieve pain but cannot reverse the disease. Patients who cannot tolerate pain usually resort to a strategy of surgical resection of the degenerated disc. However, the surgical removal of IVDD can affect the stability of adjacent discs. Furthermore, the probability of the reherniation of the intervertebral disc (IVD) after surgery is as high as 21.2%. Strategies based on tissue engineering to deliver stem cells for the regeneration of nucleus purposes (NP) and annulus fibrosus (AF) have been extensively studied. The developed biomaterials not only locally withstand the pressure of the IVD but also lay the foundation for the survival of stem cells. However, the structure of IVDs does not provide sufficient nutrients for delivered stem cells. The role of immune mechanisms in IVDD has recently become clear. In IVDD, the IVD that was originally in immune privilege prevents the attack of immune cells (mainly effector T cells and macrophages) and aggravates the disease. Immune regulatory and inflammatory factors released by effector T cells, macrophages, and the IVD further aggravate IVDD. Reversing IVDD by regulating the inflammatory microenvironment is a potential approach for the treatment of the disease. However, the biological factors modulating the inflammatory microenvironment easily degrade in vivo. It makes it possible for different biomaterials to modulate the inflammatory microenvironment to repair IVDD. In this review, we have discussed the structures of IVDs and the immune mechanisms underlying IVDD. We have described the immune mechanisms elicited by different biological factors, including tumor necrosis factors, interleukins, transforming growth factors, hypoxia-inducible factors, and reactive oxygen species in IVDs. Finally, we have discussed the biomaterials used to modulate the inflammatory microenvironment to repair IVDD and their development