Relationship, Loyalty, and Marketing -- a Correlation Study of Taiwan Hotel Customers' Perspectives

The purpose of the study was to validate customer equity theory by relating the marketing drivers to the generation of true customer loyalty among the patrons of a five-star hotel in Taipei, Taiwan. The study was guided by three objectives: (a) to determine the relationship between marketing drivers related to customer equity theory and customer loyalty, (b) to determine the profiles of customers according to the antecedents and behavioral outcomes of customer loyalty, and (c) to determine the association between the customer loyalty and the customer demographic profiles. The survey method would use self-administered questionnaires. A two-stage sampling approach including convenient sampling and systematic random sampling method was conducted. Paired samples t-test, regression analysis, cluster analysis, multiple variances of analysis in connected with discriminate analysis, one way of variance analysis, and important-performance analysis (IPA) were employed in the data analysis. The findings of empirical tests supported the conclusion that fundamental and marketing strategies related to customer equity theory increased attitudinal and behavioral loyalty. There was no linear relationship on proportion of stay from the fundamental marketing strategy and the progressive marketing strategy. Customers in the hotel could be classified in terms of attitudinal loyalty (trust, commitment, and switching cost) and behavioral loyalty (word-of-mouth endorsement, cooperation, and proportion of visit) into four segments of customer loyalty: latent (27.5%), true (48.8%), low (19.1%), and spurious (4.6%). The customers' demographic variables could not be associated with the segments of customer loyalty, attitudinal loyalty and behavioral loyalty. The managerial implication is that profiling customer developed the marketing strategies that further influence the shifts of the segments of markets. The theoretical implications indicated that the empirical results was not completely supported by customer equity theory and might be an example of dissonance theory.School of Hotel and Restaurant Administratio

Dynamic Change of Gut Microbiota During Porcine Epidemic Diarrhea Virus Infection in Suckling Piglets

Porcine epidemic diarrhea (PED) is a disease that has a devastating effect on livestock. Currently, most studies are focused on comparing gut microbiota of healthy piglets and piglets with PED, resulting in gut microbial populations related to dynamic change in diarrheal piglets being poorly understood. The current study analyzed the characteristics of gut microbiota in porcine epidemic diarrhea virus (PEDV)-infected piglets during the suckling transition stage. Fresh fecal samples were collected from 1 to 3-week-old healthy piglets (n = 20) and PEDV infected piglets (n = 18) from the same swine farm. Total DNA was extracted from each sample and the V3–V4 hypervariable region of the 16S rRNA gene was amplified and sequenced using the Illumina MiSeq platform. Statistically significant differences were observed in bacterial diversity and richness between the healthy and diarrheal piglets. Principal coordinates analysis (PCoA) showed structural segregation between diseased and healthy groups, as well as among 3 different age groups. The abundance of Escherichia-Shigella, Enterococcus, Fusobacterium, and Veillonella increased due to dysbiosis induced by PEDV infection. Notably, there was a remarkable age-related increase in Fusobacterium and Veillonella in diarrheal piglets. Certain SCFA-producing bacteria, such as Ruminococcaceae_UCG-002, Butyricimonas, and Alistipes, were shared by all healthy piglets, but were not identified in various age groups of diarrheal piglets. In addition, significant differences were observed between clusters of orthologous groups (COG) functional categories of healthy and PEDV-infected piglets. Our findings demonstrated that PEDV infection caused severe perturbations in porcine gut microbiota. Therefore, regulating gut microbiota in an age-related manner may be a promising method for the prevention or treatment of PEDV

Stable oxygen and carbon isotopes from the planktonic foraminifera Neogloboquadrina pachyderma in the Western Arctic surface sediments: Implications for water mass distribution

Neogloboquadrina pachyderma is the most abundant planktonic foraminifera species found in the modern polar oceans. The δ18O and δ13C of N. pachyderma from the Western Arctic Ocean sediments were analyzed to reveal the implications of the proxies to environmental changes. The ±18O from N. pachyderma in the Chukchi Sea reflect the water mass distribution in this area. Heavier ±18O values were found along the Anadyr Current (AC) and lighter values in the central and eastern Chukchi Sea. These may reflect the freshwater signal from the Alaska Coastal Current (ACC) and Bering Sea Shelf Water (BSSW). The light δ18O signature in the high Arctic basin comes from the freshwater stored in the Arctic surface layer. The δ13C distribution pattern in the Chukchi Sea is also influenced by the current system. High primary productivity along the AC results in heavy δ13C. The relatively low primary productivity and the freshwater component from the BSSW and ACC may be the reason for this light δ13C signal in the central and eastern Chukchi Sea. Our data reveal the importance of well ventilated Pacific Water through the Chukchi Sea into the Arctic Ocean

Late Quaternary terrigenous sedimentation in the Western Arctic Ocean as exemplified by a sedimentary record from the Alpha Ridge

Terrigenous components in sediment core B84A from the Alpha Ridge, Western Arctic Ocean, have been investigated to reconstruct Mid to Late Quaternary variations in sedimentation, provenance, and related climate changes. The core stratigraphy, evaluated by a combination of variations in Mn content, color cycles, foraminiferal abundance, and lithological correlation, extends back to estimated Marine Isotope Stage 12. Twelve Ice Rafted Detritus (IRD, >250 ¹m) events were identified and interpreted to mostly occur during deglaciation. The Canadian Arctic, which was covered by ice sheets during glacial periods, is suggested to be the major source region. The IRD events likely indicate the collapses of ice sheets, possibly in response to abrupt climate changes. Grain size analysis of B84A indicates sedimentologically sensitive components in core B84A in the 4{9 ¹m and 19{53 ¹m silt subfractions, which are inferred to be mainly transported by currents and sea ice, respectively. Down core variability of these two fractions may indicate changes in ice drift and current strength. In accordance with previous studies in the central Arctic Ocean, the average sedimentation rate in core B84A is about 0.4 cm*ka(-1). Compared with the relatively high sedimentation rates on the margins, sedimentation in the central Arctic Ocean is limited by sea ice cover and the correspondingly low bioproductivity, as well as the long distance from source regions of terrigenous sediment

Comparison of Oropharyngeal Microbiota in Healthy Piglets and Piglets With Respiratory Disease

Porcine respiratory disease (PRD) is responsible for severe economic losses in the swine industry worldwide. Our objective was to characterize the oropharyngeal microbiota of suckling piglets and compare the microbiota of healthy piglets and piglets with PRD. Oropharyngeal swabs were collected from healthy (Healthy_A, n = 6; Healthy_B, n = 4) and diseased (PRD_A, n = 18; PRD_B, n = 5) piglets at 2–3 weeks of age from two swine farms in Guangdong province, China. Total DNA was extracted from each sample and the V3-V4 hypervariable region of the 16S rRNA gene was amplified and sequenced using the Illumina MiSeq platform. No statistically significant differences were observed in bacterial diversity and richness between the healthy and PRD groups in the two farms except for Shannon index in farm A. Principal coordinates analysis (PCoA) showed structural segregation between diseased and healthy groups and between groups of different farms. Among all samples, the phyla Firmicutes, Proteobacteria, and Bacteroidetes were predominant. At the genus level, Streptococcus, Lactobacillus, and Actinobacillus were the core genera in the oropharynx of healthy piglets from the two farms. Significant differences in bacterial taxa were found when the microbiota was compared regarding the health status. In farm A, the percentages of Moraxella and Veillonella were higher in the PRD group, while only Porphyromonas was significantly increased in the PRD group in farm B (p < 0.05). Compared to PRD groups, statistically significant predominance of Lactobacillus was observed in the healthy groups from both farms (p < 0.05). Our findings revealed that Moraxella, Veillonella, and Porphyromonas may play a potential role in PRD and Lactobacillus may have a protective role against respiratory diseases

ICIs-Related Cardiotoxicity in Different Types of Cancer

Immune checkpoint inhibitors (ICIs) are rapidly developing immunotherapy cancer drugs that have prolonged patient survival. However, ICIs-related cardiotoxicity has been recognized as a rare, but fatal, consequence. Although there has been extensive research based on different types of ICIs, these studies have not indicated whether cardiotoxicity is specific to a type of cancer. Therefore, we conducted a systematic review to analyze a variety of ICIs-related cardiotoxicity, focusing on different types of cancer. We found that the incidence of ICIs-related cardiac adverse events (CAEs) and common cardiotoxic manifestations vary with cancer type. This inspired us to explore the underlying mechanisms to formulate targeted clinical strategies for maintaining the cardiovascular health of cancer patients

An expanded database of Southern Hemisphere surface sediment dinoflagellate cyst assemblages and their oceanographic affinities

Dinoflagellate cyst assemblages present a valuable proxy to infer paleoceanographic conditions, yet factors influencing geographic distributions of species remain largely unknown, especially in the Southern Ocean. Strong lateral transport, sea-ice dynamics, and a sparse and uneven geographic distribution of surface sediment samples have limited the use of dinocyst assemblages as a quantitative proxy for paleo-environmental conditions such as sea surface temperature (SST), nutrient concentrations, salinity, and sea ice (presence). In this study we present a new set of surface sediment samples (nCombining double low line66) from around Antarctica, doubling the number of Antarctic-proximal samples to 100 (dataset wsi_100) and increasing the total number of Southern Hemisphere samples to 655 (dataset sh_655). Additionally, we use modelled ocean conditions and apply Lagrangian techniques to all Southern Hemisphere sample stations to quantify and evaluate the influence of lateral transport on the sinking trajectory of microplankton and, with that, to the inferred ocean conditions. k-means cluster analysis on the wsi_100 dataset demonstrates the strong affinity of Selenopemphix antarctica with sea-ice presence and of Islandinium spp. with low-salinity conditions. For the entire Southern Hemisphere, the k-means cluster analysis identifies nine clusters with a characteristic assemblage. In most clusters a single dinocyst species dominates the assemblage. These clusters correspond to well-defined oceanic conditions in specific Southern Ocean zones or along the ocean fronts. We find that, when lateral transport is predominantly zonal, the environmental parameters inferred from the sea floor assemblages mostly correspond to those of the overlying ocean surface. In this case, the transport factor can thus be neglected and will not represent a bias in the reconstructions. Yet, for some individual sites, e.g. deep-water sites or sites under strong-current regimes, lateral transport can play a large role. The results of our study further constrain environmental conditions represented by dinocyst assemblages and the location of Southern Ocean frontal systems

Pseudorabies gD protein protects mice and piglets against lethal doses of pseudorabies virus

IntroductionPseudorabies (PR) is a highly contagious viral disease caused by the pseudorabies virus (PRV), which can cause disease in a wide range of domestic and wild animals. Studies have shown that new mutant strains have emerged in pig farms in many regions and that commercial inactivated and live attenuated vaccines are becoming less effective at protecting pigs.MethodsPorcine pseudorabies glycoprotein D (gD) gene (GenBank: QEY95774.1) with hexa-His tag to the C terminus for further purification processes was cloned into the lentiviral expression plasmid pLV-CMV-eGFP by restriction enzyme, the resulting plasmid was designated as pLV-CMV-gD. HEK-293T cells with robust and stable expression of recombinant gD protein was established by infection with recombinant lentivirus vector pLV-CMV-gD. We expressed porcine pseudorabies virus gD protein using HEK-293T cells.ResultsWe describe in this study that individual gD proteins produced by a mammalian cell expression system are well immunogenic and stimulate high levels of PRV-specific and neutralizing antibodies in mice and piglets. All mice and piglets survived lethal doses of PRV, significantly reducing the amount of PRV virus in piglets’ lymph nodes, lungs, spleen, and other tissues. It also significantly reduced the time cycle and amount of viral excretion from piglets to the environment through the nasal and anal cavities.DiscussionThe results suggest that PRV gD protein is expected to be a potential candidate for the preparation of genetically engineered PR vaccines for the prevention of PRV infection and the control of PR epidemics

Foraminifer datums, opal content and opal accumulation rate of ODP Site 184-1143 sediments

Since the 1970s, Ocean Drilling Program (ODP) and Deep Sea Drilling Program (DSDP) studies have documented high accumulations of biogenic silica and carbonate in the late Miocene-early Pliocene Indian-Pacific Ocean. This high biogenic productivity event, or the "Biogenic Bloom Event," has been dated from 9.0 to 3.5 Ma (Leinen, 1979, doi:10.1130/0016-7606(1979)902.0.CO;2; Theyer et al., 1985, doi:10.2973/dsdp.proc.85.133.1985; Farrell et al., 1995, doi:10.2973/odp.proc.sr.138.143.1995; Dickens and Owen, 1996, doi:10.1016/0377-8398(95)00054-2, 1999, doi:10.1016/S0025-3227(99)00057-2; Dickens and Barron, 1997, doi:10.1016/S0377-8398(97)00003-0; Berger et al., 1993, doi:10.2973/odp.proc.sr.130.051.1993). It is unknown, however, whether the Biogenic Bloom Event existed in the South China Sea (SCS). High-quality Cenozoic sediment cores taken from the SCS during ODP Leg 184 provide an opportunity to investigate this question. The purpose of this study is to trace and illustrate the change in biogenic productivity in the southern SCS since the late Miocene and the Biogenic Bloom Event in terms of the content and accumulation rate of opal and carbonate at Site 1143