4,480 research outputs found
Intra-articular injection of the cyclooxygenase-2 inhibitor parecoxib attenuates osteoarthritis progression in anterior cruciate ligament-transected knee in rats: role of excitatory amino acids
SummaryObjectiveOur present study examined the effect of intra-articular cyclooxygenase-2 (COX-2) inhibitor parecoxib on osteoarthritis (OA) progression and the concomitant changes in excitatory amino acids' (EAAs) levels of the anterior cruciate ligament-transected (ACLT) knee joint dialysates.MethodsOA was induced in Wistar rats by anterior cruciate ligament transection of the knee of one hindlimb, the other was left unoperated and untreated. Rats were placed into four groups: Group ACLT/P received intra-articular parecoxib injection (100ÎŒg) in the ACLT knee once a week for 5 consecutive weeks starting at 8 weeks after surgery. Group ACLT/S received the same procedure as group ACLT/P with saline injection instead. NaĂŻve (NaĂŻve/P) rats received only intra-articular parecoxib injection in one knee once a week for 5 consecutive weeks without surgery. The sham-operated rats underwent arthrotomy only without treatment. Twenty weeks after surgery, knee joint dialysates were collected and EAAs' concentration was assayed by high-performance liquid chromatography, and gross morphology and histopathology (Mankin and synovitis grading) were examined on the medial femoral condyles and synovia.ResultsParecoxib alone had no effect on cartilage and synovium of normal knees in NaĂŻve/P rats. In ACLT/P rats, parecoxib treatment showed a significant inhibition of cartilage degeneration of the medial femoral condyle at both the macroscopic level (1.15±0.17 vs 2.55±0.12, P<0.05) and the Mankin scores (3.03±0.28 vs 8.82±0.43, P<0.05). Intra-articular parecoxib injection also suppressed the synovial inflammation of ACLT joint compared to the ACLT/S group (3.92±0.41 vs 9.25±0.32, P<0.05). Moreover, glutamate and aspartate levels were also significantly reduced in the ACLT/P group compared to the ACLT/S group by parecoxib treatment (91.2±9.4% vs 189.5±17.0%, P<0.05 and 98.2±11.6% vs 175.3±12.4%, P<0.05, respectively).ConclusionThis study shows that intra-articular injection of COX-2 inhibitor parecoxib inhibits the ACLT-induced OA progression; it was accompanied by a reduction of glutamate and aspartate concentration in the ACLT joint dialysates. From our present results, we suggested that intra-articular parecoxib injection, in addition to the anti-inflammatory effect, inhibiting the EAAs' release, may also play a role in inhibiting the traumatic knee injury induced OA progression
Undecidable properties of self-affine sets and multi-tape automata
We study the decidability of the topological properties of some objects
coming from fractal geometry. We prove that having empty interior is
undecidable for the sets defined by two-dimensional graph-directed iterated
function systems. These results are obtained by studying a particular class of
self-affine sets associated with multi-tape automata. We first establish the
undecidability of some language-theoretical properties of such automata, which
then translate into undecidability results about their associated self-affine
sets.Comment: 10 pages, v2 includes some corrections to match the published versio
Mass and dust in the disk of a spiral lens galaxy
Gravitational lensing is a potentially important probe of spiral galaxy
structure, but only a few cases of lensing by spiral galaxies are known. We
present Hubble Space Telescope and Magellan observations of the two-image
quasar PMN J2004-1349, revealing that the lens galaxy is a spiral galaxy. One
of the quasar images passes through a spiral arm of the galaxy and suffers 3
magnitudes of V-band extinction. Using simple lens models, we show that the
mass quadrupole is well-aligned with the observed galaxy disk. A more detailed
model with components representing the bulge and disk gives a bulge-to-disk
mass ratio of 0.16 +/- 0.05. The addition of a spherical dark halo, tailored to
produce an overall flat rotation curve, does not change this conclusion.Comment: ApJ, in press [9pp, 7 figs
The influence of feature selection methods on accuracy, stability and interpretability of molecular signatures
Motivation: Biomarker discovery from high-dimensional data is a crucial
problem with enormous applications in biology and medicine. It is also
extremely challenging from a statistical viewpoint, but surprisingly few
studies have investigated the relative strengths and weaknesses of the plethora
of existing feature selection methods. Methods: We compare 32 feature selection
methods on 4 public gene expression datasets for breast cancer prognosis, in
terms of predictive performance, stability and functional interpretability of
the signatures they produce. Results: We observe that the feature selection
method has a significant influence on the accuracy, stability and
interpretability of signatures. Simple filter methods generally outperform more
complex embedded or wrapper methods, and ensemble feature selection has
generally no positive effect. Overall a simple Student's t-test seems to
provide the best results. Availability: Code and data are publicly available at
http://cbio.ensmp.fr/~ahaury/
Black Hole and Neutron Star Transients in Quiescence
We consider the X-ray luminosity difference between neutron star and black
hole soft X-ray transients (NS and BH SXTs) in quiescence. The current
observational data suggest that BH SXTs are significantly fainter than NS SXTs.
The luminosities of quiescent BH SXTs are consistent with the predictions of
binary evolution models for the mass transfer rate if (1) accretion occurs via
an ADAF in these systems and (2) the accreting compact objects have event
horizons. The luminosities of quiescent NS SXTs are not consistent with the
predictions of ADAF models when combined with binary evolution models, unless
most of the mass accreted in the ADAF is prevented from reaching the neutron
star surface. We consider the possibility that mass accretion is reduced in
quiescent NS SXTs because of an efficient propeller and develop a model of the
propeller effect that accounts for the observed luminosities. We argue that
modest winds from ADAFs are consistent with the observations while strong winds
are probably not.Comment: LateX, 37 pages, 7 figures; Accepted for publication in The
Astrophysical Journa
Photodynamic drug delivery enhancement in tumours does not depend on leukocyte-endothelial interaction in a human mesothelioma xenograft modelâ
OBJECTIVES The pre-treatment of tumour neovessels by low-level photodynamic therapy (PDT) improves the distribution of concomitantly administered systemic chemotherapy. The mechanism by which PDT permeabilizes the tumour vessel wall is only partially known. We have recently shown that leukocyte-endothelial cell interaction is essential for photodynamic drug delivery to normal tissue. The present study investigates whether PDT enhances drug delivery in malignant mesothelioma and whether it involves comparable mechanisms of actions. METHODS Human mesothelioma xenografts (H-meso-1) were grown in the dorsal skinfold chambers of 28 nude mice. By intravital microscopy, the rolling and recruitment of leukocytes were assessed in tumour vessels following PDT (VisudyneÂź 400ÎŒg/kg, fluence rate 200mW/cm2and fluence 60J/cm2) using intravital microscopy. Likewise, the distribution of fluorescently labelled macromolecular dextran (FITC-dextran, MW 2000kDa) was determined after PDT. Study groups included no PDT, PDT, PDT plus a functionally blocking anti-pan-selectin antibody cocktail and PDT plus isotype control antibody. RESULTS PDT significantly enhanced the extravascular accumulation of FITC-dextran in mesothelioma xenografts, but not in normal tissue. PDT significantly increased leukocyte-endothelial cell interaction in tumour. While PDT-induced leukocyte recruitment was significantly blunted by the anti-pan-selectin antibodies in the tumour xenograft, this manipulation did not affect the PDT-induced extravasation of FITC-dextran. CONCLUSIONS Low-level PDT pre-treatment selectively enhances the uptake of systemically circulating macromolecular drugs in malignant mesothelioma, but not in normal tissue. Leukocyte-endothelial cell interaction is not required for PDT-induced drug delivery to malignant mesotheliom
The multiple solutions of self-consistency condition in Walecka model and the validity of the Brown-Rho scaling law
We investigate the self-consistency condition (SCC) of mean-field theory in
Walecka model and find that the solutions of the SCC are multiple at high
temperature and chemical potential. Using the effective Lagrangian approach, we
study medium effects on the meson mass by taking into account of
vacuum effects. We show that the meson mass decreases with both
temperature and chemical potential with a general tendency, while near the
critical point several meson masses become degenerate due to the
multiple solutions of the SCC. We check the validity of Brown-Rho scaling law
in this case. Finally, we calculate the thermodynamic potential and prove that
the multiple solutions of the SCC result from a first-order phase transition of
nuclear matter in the Walecka model at high temperature and chemical potential.Comment: 10 pages in Revtex with 9 figure
Investigating electron interacting dark matter
Some extensions of the Standard Model provide Dark Matter candidate particles
which can have a dominant coupling with the lepton sector of the ordinary
matter. Thus, such Dark Matter candidate particles () can be directly
detected only through their interaction with electrons in the detectors of a
suitable experiment, while they are lost by experiments based on the rejection
of the electromagnetic component of the experimental counting rate. These
candidates can also offer a possible source of the 511 keV photons observed
from the galactic bulge. In this paper this scenario is investigated. Some
theoretical arguments are developed and related phenomenological aspects are
discussed. Allowed intervals and regions for the characteristic
phenomenological parameters of the considered model and of the possible
mediator of the interaction are also derived considering the DAMA/NaI data.Comment: 16 pages, 6 figures. Accepted for publication in PRD. One typo
correcte
Transition Between Ground State and Metastable States in Classical 2D Atoms
Structural and static properties of a classical two-dimensional (2D) system
consisting of a finite number of charged particles which are laterally confined
by a parabolic potential are investigated by Monte Carlo (MC) simulations and
the Newton optimization technique. This system is the classical analog of the
well-known quantum dot problem. The energies and configurations of the ground
and all metastable states are obtained. In order to investigate the barriers
and the transitions between the ground and all metastable states we first
locate the saddle points between them, then by walking downhill from the saddle
point to the different minima, we find the path in configurational space from
the ground state to the metastable states, from which the geometric properties
of the energy landscape are obtained. The sensitivity of the ground-state
configuration on the functional form of the inter-particle interaction and on
the confinement potential is also investigated
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