Impacts of Rice Field Winter Planting on Soil Organic Carbon and Carbon Management Index

To tackle with the problem of prevailing farmland abandonment in winter, 5 treatments includes Chinese milk vetch-double cropping rice (CRR), rape-double cropping rice (RRR), garlic-double cropping rice (GRR), winter crop multiple cropping rotation (ROT), winter fallow control (WRR) were set up. By measuring soil total organic carbon, active organic carbon and its components and calculating the soil carbon pool management index in 0~15 cm and 15~30 cm soil layers in the early and late rice ripening stage. The effects of different winter planting patterns on the changes of soil organic carbon and carbon pool management index were discussed. In order to provide theoretical basis for the optimization and adjustment of winter planting pattern of double cropping rice field in the middle reaches of Yangtze River. The results showed that soil total organic carbon, active organic carbon and its components in different winter cropping patterns were increased, and ROT and CRR treatments were more beneficial to the accumulation of soil total organic carbon, active organic carbon and its components as well as the improvement of soil carbon pool management index, which should be preferred in the adjustment of cropping patterns

OR-023 Physical evaluation of 6-7 years old female preselected tennis players

Objective Through testing and analysis the characteristics of body shape，body composition，bone growth and physical fitness，hemoglobin, testosterone of 6-7 years old female preselected tennis players，the study aim was to provide reference bases for the early selection of female tennis players. Methods A total of 75 female preselected tennis players(initial selection by the coaches) aged from 6 to 7 years were came from Hebei, Hubei, Qinghai and Inner Mongolia province, who came to Research Center for Heath related Physical Fitness Evaluation of Guangzhou Sport University for physical fitness test from July 2016 to July 2018. The height, weight, length of upper limbs, length of lower limbs, iliac width, shoulder width, body fat, muscle mass, bone age，bone mass density(BMD), anaerobic power and PWC170, reaction time, vertical jump, grip strength, hemoglobin, testosterone were measured using related instruments and methods, and calculated derived indicators BMI, iliac width/shoulder width. Data were compared with the national standard of physical health of students and/or evaluated by deviation method，and correlation had been analysed among physical parameters. Results 1)The 75 female preselected tennis players’ aged from 6 to 7 years height and weight were 128.10±5.32cm and 25.70±3.87kg，and there are 47 girls height upper medium grade level, 60% of which weight was at a moderate level, their BMI were 15.48±1.50kg/m2，and all in the normal range, iliac width/shoulder width ×100 was 76.52±7.00, 70.7% 0f which was above medium grade level, the upper and lower limbs were 54.28±3.60cm and 71.68±5.26cm, girls’ PBF were 21.03±6.44, muscle weight were 18.94±3.00kg, BMD were 2.04±2.20, and no low bone strength were fund; Anaerobic power of all female preselected tennis players were 135.93±31.65kg.cm, and the values of the PWC170 relative weight were 10.79±2.56kg.m/min.kg, reaction time were 0.628±0.128s, vertical jump were 21.13±4.95cm, the grip of right and left hand were 10.36±2.15kg and 10.06±2.40kg, the physical fitness parameters above in the upper middle class were more girls than the lower middle class; The hemoglobin content was 132.15±8.70g/L, which was above the normal level (110 -160g/L), the serum testosterone concentration was 1.52±1.20umol/L, which was much higher than that of normal girls (0-0.7umol/L). 2) When age was controlled, there was negative correlation between T and PFB, vertical jump and body weight, PWC170 and reaction time(P＜0.05), and there was positive correlation between hemoglobin and muscle weight(P＜0.05), height, and vertical jump(P＜0.01), muscle weight and anaerobic power(P<0.01)，anaerobic power and height，weight，BMI，upper and lower limbs(P<0.01, P<0.01, P<0.01, P<0.05, P<0.01)，PWC170 and vertical jump，the grip of right and left hand(P<0.01, P<0.05, P<0.05), vertical jump and upper, lower limbs, iliac width/shoulder width(P<0.01, P<0.01, P<0.01). Conclusions 75 female aged from 6 to 7 years old preselected tennis players’ body shape, physical fitness, physiological and biochemical function were superior to peers, and in those parameters，there were more people in upper middle grade than  lower middle grade. There is a certain correlation between body composition, shape and fitness of female preselected tennis players’ aged from 6 to 7 years

Gene expression profile based classification models of psoriasis

AbstractPsoriasis is an autoimmune disease, which symptoms can significantly impair the patient's life quality. It is mainly diagnosed through the visual inspection of the lesion skin by experienced dermatologists. Currently no cure for psoriasis is available due to limited knowledge about its pathogenesis and development mechanisms. Previous studies have profiled hundreds of differentially expressed genes related to psoriasis, however with no robust psoriasis prediction model available. This study integrated the knowledge of three feature selection algorithms that revealed 21 features belonging to 18 genes as candidate markers. The final psoriasis classification model was established using the novel Incremental Feature Selection algorithm that utilizes only 3 features from 2 unique genes, IGFL1 and C10orf99. This model has demonstrated highly stable prediction accuracy (averaged at 99.81%) over three independent validation strategies. The two marker genes, IGFL1 and C10orf99, were revealed as the upstream components of growth signal transduction pathway of psoriatic pathogenesis

The goose genome sequence leads to insights into the evolution of waterfowl and susceptibility to fatty liver

BACKGROUND: Geese were domesticated over 6,000 years ago, making them one of the first domesticated poultry. Geese are capable of rapid growth, disease resistance, and high liver lipid storage capacity, and can be easily fed coarse fodder. Here, we sequence and analyze the whole-genome sequence of an economically important goose breed in China and compare it with that of terrestrial bird species. RESULTS: A draft sequence of the whole-goose genome was obtained by shotgun sequencing, and 16,150 protein-coding genes were predicted. Comparative genomics indicate that significant differences occur between the goose genome and that of other terrestrial bird species, particularly regarding major histocompatibility complex, Myxovirus resistance, Retinoic acid-inducible gene I, and other genes related to disease resistance in geese. In addition, analysis of transcriptome data further reveals a potential molecular mechanism involved in the susceptibility of geese to fatty liver disease and its associated symptoms, including high levels of unsaturated fatty acids and low levels of cholesterol. The results of this study show that deletion of the goose lep gene might be the result of positive selection, thus allowing the liver to adopt energy storage mechanisms for long-distance migration. CONCLUSIONS: This is the first report describing the complete goose genome sequence and contributes to genomic resources available for studying aquatic birds. The findings in this study are useful not only for genetic breeding programs, but also for studying lipid metabolism disorders. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13059-015-0652-y) contains supplementary material, which is available to authorized users

Metagenomic Insights Into a Cellulose-Rich Niche Reveal Microbial Cooperation in Cellulose Degradation

BackgroundCellulose is the most abundant organic polymer mainly produced by plants in nature. It is insoluble and highly resistant to enzymatic hydrolysis. Cellulolytic microorganisms that are capable of producing a battery of related enzymes play an important role in recycling cellulose-rich plant biomass. Effective cellulose degradation by multiple synergic microorganisms has been observed within a defined microbial consortium in the lab culture. Metagenomic analysis may enable us to understand how microbes cooperate in cellulose degradation in a more complex microbial free-living ecosystem in nature.ResultsHere we investigated a typical cellulose-rich and alkaline niche where constituent microbes survive through inter-genera cooperation in cellulose utilization. The niche has been generated in an ancient paper-making plant, which has served as an isolated habitat for over 7 centuries. Combined amplicon-based sequencing of 16S rRNA genes and metagenomic sequencing, our analyses showed a microbial composition with 6 dominant genera including Cloacibacterium, Paludibacter, Exiguobacterium, Acetivibrio, Tolumonas, and Clostridium in this cellulose-rich niche; the composition is distinct from other cellulose-rich niches including a modern paper mill, bamboo soil, wild giant panda guts, and termite hindguts. In total, 11,676 genes of 96 glucoside hydrolase (GH) families, as well as 1,744 genes of carbohydrate transporters were identified, and modeling analysis of two representative genes suggested that these glucoside hydrolases likely evolved to adapt to alkaline environments. Further reconstruction of the microbial draft genomes by binning the assembled contigs predicted a mutualistic interaction between the dominant microbes regarding the cellulolytic process in the niche, with Paludibacter and Clostridium acting as helpers that produce endoglucanases, and Cloacibacterium, Exiguobacterium, Acetivibrio, and Tolumonas being beneficiaries that cross-feed on the cellodextrins by oligosaccharide uptake.ConclusionThe analysis of the key genes involved in cellulose degradation and reconstruction of the microbial draft genomes by binning the assembled contigs predicted a mutualistic interaction based on public goods regarding the cellulolytic process in the niche, suggesting that in the studied microbial consortium, free-living bacteria likely survive on each other by acquisition and exchange of metabolites. Knowledge gained from this study will facilitate the design of complex microbial communities with a better performance in industrial bioprocesses

Formation of TiC via interface reaction between diamond grits and Sn-Ti alloys at relatively low temperatures

In this paper, interfacial reaction between diamond grit and Sn-6Ti alloy was systematically studied at brazing temperatures from 600 to 1030 °C. A thin and uniform layer of scallop-like nano-sized TiC grains was formed after brazing for 30 min at 600 °C, and interfacial TiC grains subsequently coarsened as brazing temperature increased to 740 and 880 °C. Strip-like columnar TiC grains in a bilayer structure was further grown as brazing temperature increased to 930 °C. After brazing at 1030 °C, a dense layer of columnar TiC grains were formed. Based on the TEM micrographs of interfacial TiC, the formation and evolution of the growth morphologies of interfacial TiC was believed to be controlled by the diffusion of C flux from diamond grits, which is dependent on the brazing temperatures

Neuraminidase and Hemagglutinin Matching Patterns of a Highly Pathogenic Avian and Two Pandemic H1N1 Influenza A Viruses

BACKGROUND: Influenza A virus displays strong reassortment characteristics, which enable it to achieve adaptation in human infection. Surveying the reassortment and virulence of novel viruses is important in the prevention and control of an influenza pandemic. Meanwhile, studying the mechanism of reassortment may accelerate the development of anti-influenza strategies. METHODOLOGY/PRINCIPAL FINDINGS: The hemagglutinin (HA) and neuraminidase (NA) matching patterns of two pandemic H1N1 viruses (the 1918 and current 2009 strains) and a highly pathogenic avian influenza A virus (H5N1) were studied using a pseudotyped particle (pp) system. Our data showed that four of the six chimeric HA/NA combinations could produce infectious pps, and that some of the chimeric pps had greater infectivity than did their ancestors, raising the possibility of reassortment among these viruses. The NA of H5N1 (A/Anhui/1/2005) could hardly reassort with the HAs of the two H1N1 viruses. Many biological characteristics of HA and NA, including infectivity, hemagglutinating ability, and NA activity, are dependent on their matching pattern. CONCLUSIONS/SIGNIFICANCE: Our data suggest the existence of an interaction between HA and NA, and the HA NA matching pattern is critical for valid viral reassortment

Variasi Temperatur Pencampuran Terhadap Parameter Marshall Pada Campuran Lapis Aspal Beton

This study was conducted to determine the effect of temperature variations on the mixing processof the asphalt concrete AC-WC (Asphalt Concrete-Wearing Course) subtle gradations in themiddle limit and lower limit of the Marshall parameters with reference to specifications of BinaMarga, 2010.From the results of experiments conducted that the optimum asphalt content is used to middle limitusing a asphalt content of 5,7% and 6,8% for the lower limit after that mixing was done usingtemperature variation of 120 o C, 130 o C, 140 o C, 150 o C, and 160 o C.To a mixture of Laston AC-WC subtle gradations middle limit grading 5,7% asphalt contentmixing temperature using a temperature of 120 o C, 130 o C, 140 o C, 150 o C, 160 o C and still meet allstandards of marshall parameters. Ideal mixing temperature variations in the middle limit ofmixing temperature 150 o C-160 o C. While the lower limit to the level of 6,8% asphalt contentmixing temperatures between 120 o C-160 o C did not meet the specifications, because the MQ valuebelow the minimum value of 250 kg / mm