63 research outputs found
High versus low protein diets to mink:postprandial plasma urea and creatinine response, osmotic load and pattern of nitrogen and electrolyte excretion
High versus low protein diets to mink:postprandial plasma urea and creatinine response, osmotic load and pattern of nitrogen and electrolyte excretion
Preservation of active incretin hormones by inhibition of dipeptidyl peptidase IV suppresses meal-induced incretin secretion in dogs
Effects of substitution of dietary protein with carbohydrate on lactation performance in the mink (Mustela vison)
Effects of substitution of dietary protein with carbohydrate on lactation performance in the mink (Mustela vison)
Epicardial adipose tissue inflammation is related to vitamin D deficiency in patients affected by coronary artery disease
Synthesis of Lipophilic Guanine N‑9 Derivatives: Membrane Anchoring of Nucleobases Tailored to Fatty Acid Vesicles
Covalent
or noncovalent surface functionalization of soft-matter
structures is an important tool for tailoring their function and stability.
Functionalized surfaces and nanoparticles have found numerous applications
in drug delivery and diagnostics, and new functionalization chemistry
is continuously being developed in the discipline of bottom-up systems
chemistry. The association of polar functional molecules, e.g., molecular
recognition agents, with soft-matter structures can be achieved by
derivatization with alkyl chains, allowing noncovalent anchoring into
amphiphilic membranes. We report the synthesis of five new guanine–N9
derivatives bearing alkyl chains with different attachment chemistries,
exploiting a synthesis pathway that allows a flexible choice of hydrophobic
anchor moiety. In this study, these guanine derivatives were functionalized
with C<sub>10</sub> chains for insertion into decanoic acid bilayer
structures, in which both alkyl chain length and attachment chemistry
determined their interaction with the membrane. Incubation of these
guanine conjugates, as solids, with a decanoic acid vesicle suspension,
showed that ether- and triazole-linked C<sub>10</sub> anchors yielded
an increased partitioning of the guanine derivative into the membranous
phase compared to directly N-9-linked saturated alkyl anchors. Decanoic
acid vesicle membranes could be loaded with up to 5.5 mol % guanine
derivative, a 6-fold increase over previous limits. Thus, anchor chemistries
exhibiting favorable interactions with a bilayer’s hydrophilic
surface can significantly increase the degree of structure functionalization
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